Abstract:
BACKGROUND:Gram-negative bacteria (GNB) are a leading cause of nosocomial infection and sepsis. Increasing multi-antibiotic resistance has left clinicians with fewer therapeutic options. Antibodies to GNB lipopolysaccharide (LPS, or endotoxin) have reduced morbidity and mortality as a result of infection and are not subject to the resistance mechanisms deployed by bacteria against antibiotics. In this phase 1 study, we administered a vaccine that elicits antibodies against a highly conserved portion of LPS with and without a CpG oligodeoxynucleotide (ODN) TLR9 agonist as adjuvant. METHODS:A vaccine composed of the detoxified LPS (dLPS) from E. coli O111:B4 (J5 mutant) non-covalently complexed to group B meningococcal outer membrane protein (OMP). Twenty healthy adult subjects received three doses at 0, 29 and 59 days of antigen (10 μg dLPS) with or without CPG 7909 (250 or 500 μg). Subjects were evaluated for local and systemic adverse effects and laboratory findings. Anti-J5 LPS IgG and IgM antibody levels were measured by electrochemiluminesence. Due to premature study termination, not all subjects received all three doses. RESULTS:All vaccine formulations were well-tolerated with no local or systemic events of greater than moderate severity. The vaccine alone group achieved a ≥ 4-fold "responder" response in IgG and IgM antibody in only one of 6 subjects. In contrast, the vaccine plus CPG 7909 groups appeared to have earlier and more sustained (to 180 days) responses, greater mean-fold increases, and a higher proportion of "responders" achieving ≥ 4-fold increases over baseline. CONCLUSIONS:Although the study was halted before all enrolled subjects received all three doses, the J5dLPS/OMP vaccine, with or without CpG adjuvant, was safe and well-tolerated. The inclusion of CpG increased the number of subjects with a ≥ 4-fold antibody response, evident even after the second of three planned doses. A vaccine comprising J5dLPS/OMP antigen with CpG adjuvant merits further investigation. CLINICAL TRIALS REGISTRATION:ClinicalTrials.gov Identifier: NCT01164514.
journal_name
Vaccinejournal_title
Vaccineauthors
Cross AS,Greenberg N,Billington M,Zhang L,DeFilippi C,May RC,Bajwa KKdoi
10.1016/j.vaccine.2015.10.072subject
Has Abstractpub_date
2015-11-27 00:00:00pages
6719-26issue
48eissn
0264-410Xissn
1873-2518pii
S0264-410X(15)01501-7journal_volume
33pub_type
杂志文章相关文献
VACCINE文献大全abstract::The ability to elicit humoral and cell-mediated immune (CMI) responses from DNA immunization by combinational use of codon optimization and C3d component of complement was evaluated in this study. DNA vaccines that express either the wild type or the codon optimized gp120 gene coding for the envelope (Env) glycoprotei...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.09.054
更新日期:2004-04-16 00:00:00
abstract::The aim of this study was to characterize the adverse events of attenuated measles vaccine in mainland China. We carried out prospective follow-up of adverse events after mass measles immunization practices, categorizing them into two groups: allergic reactions and serious adverse events. The incidence of anaphylaxis ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.106
更新日期:2011-04-18 00:00:00
abstract::Although a highly effective vaccine against smallpox, vaccinia virus (VV) is not without adverse events, some of which can be life-threatening, particularly in immunocompromised individuals. We have recently demonstrated that the immunogenicity and protective efficacy of Dryvax(®) in immunocompetent mice is preserved ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.10.039
更新日期:2010-12-16 00:00:00
abstract:OBJECTIVE:To assess the efficacy and safety of whole-cell and acellular pertussis vaccines administered to children singly or within diphtheria, tetanus and pertussis (DTP) vaccines. DATA SOURCES:We searched the Cochrane Library, MEDLINE, EMBASE, Biological Abstracts and Science Citation Index to December 2001. Specia...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(02)00770-3
更新日期:2003-05-16 00:00:00
abstract:INTRODUCTION:Widespread introduction of pneumococcal conjugated vaccines (PCVs) impacted on invasive pneumococcal disease (IPD). However, IPD reduction may not be similar in all outcomes within IPD. We assessed PCV7/PCV13 impact on pneumococcal meningitis, bacteremic pneumonia (BP) and other (non-meningitis, non-pneumo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.05.059
更新日期:2018-08-28 00:00:00
abstract::Intranasal (i.n.) immunization is an effective regimen for the prophylaxis of respiratory diseases in early life. The aim of this study was to assess the need for nasal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in induction of protective immunity following mucosal vaccination of infant mice. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.01.027
更新日期:2008-03-17 00:00:00
abstract::A robust vaginal immune response is considered essential for an effective prophylactic vaccine that prevents transmission of HIV and other sexually acquired diseases. Considerable attention has recently focused on the potential of vaginally administered vaccines as a means to induce such local immunity. However, the p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.004
更新日期:2012-04-05 00:00:00
abstract:BACKGROUND:Increased travel to exotic destinations around the world is escalating the risk of exposure to animal-associated injuries with a risk of acquiring rabies. METHODS:We have examined data reported to GeoSentinel Surveillance Network to highlight characteristics of animal-associated injuries in travellers. RES...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.12.034
更新日期:2007-03-30 00:00:00
abstract::In Italy systematic mandatory tetanus immunization of children started in 1968. In a national sample of 241 young males born after 1968 the prevalence of non-immune subjects was 11.2% (5.5% in the North-Centre, 15.9% in the South-Islands; p < 0.02). Comparing these data with the corresponding figure obtained in a prev...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90381-7
更新日期:1993-01-01 00:00:00
abstract::Immunization protocols based on priming with plasmid DNA and boosting with recombinants of vaccinia virus (rVV) encoding the same antigen offer great promise for the prevention and treatment of many parasitic and viral infections for which conventional vaccination has little or no effect. To overcome some of the poten...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00450-4
更新日期:2002-12-13 00:00:00
abstract:INTRODUCTION:The burden of infection in communities determines the spread of rotavirus infection and disease in susceptible populations. This study reports rotavirus infection and disease in a community based birth cohort in Vellore. METHODS:Bimonthly surveillance and diarrheal stool were collected from 452 children e...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.03.039
更新日期:2014-08-11 00:00:00
abstract::Although concerns about safety of influenza vaccination during pregnancy have been raised in the past, vaccination of pregnant women was recommended in many countries during the 2009 A/H1N1 pandemic influenza. A retrospective cohort study was conducted to evaluate the risk of adverse maternal, fetal and neonatal outco...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.03.041
更新日期:2015-05-05 00:00:00
abstract::Immunization of hairless mice with a TK-, ACVr, non-pathogenic herpes simplex virus (HSV) type 1 mutant protected the mice against reinfection with lethal doses of the parental pathogenic HSV strain. The protection conferred by the mutant against the establishment of latency after reinfection with the parental strain ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(84)90089-6
更新日期:1984-09-01 00:00:00
abstract::To circumvent limitations of poor antigen presentation and immunogenicity of DNA vaccines that target induction of CD8(+) T cell immunity, we have generated single chain MHC I trimers (MHC I SCTs) composed of a single polypeptide chain with a linear composition of antigenic peptide, β2-microglobulin, and heavy chain o...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.01.012
更新日期:2012-03-09 00:00:00
abstract::Vaccination is considered the most cost-effective approach to preventing infectious diseases, yet better formulations and delivery methods for efficient distribution and administration of vaccines are needed, especially for low-resource settings. A fast-dissolving tablet (FDT) that could be packaged in a compact stack...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.08.010
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:In the Netherlands, a relatively low varicella disease burden compared to other European countries is observed within routine surveillance. To validate this, we estimated the varicella-related consultation rate using The Integrated Primary Care Information database. METHODS:In this retrospective cohort stud...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.034
更新日期:2014-06-12 00:00:00
abstract::Peptidoglycan monomer (PGM) is a natural compound of bacterial origin. It is a non-toxic, non-pyrogenic, water-soluble immunostimulator potentiating humoral immune response to ovalbumin (OVA) in mice. It is fast degraded and its metabolic products-the pentapeptide (PP) and the disaccharide (DS)-are excreted from the m...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00547-9
更新日期:2003-02-14 00:00:00
abstract:BACKGROUND:Surveillance data on invasive pneumococcal disease (IPD) in Denmark (1999-2014) was analysed regarding the incidence and age-distribution due to ten selected non-PCV serotypes (10-Non-PCV). The effect of PCV-7 and PCV-13 vaccines on the 10-Non-PCV IPD incidence was examined. METHODS:IPD cases caused by sero...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.056
更新日期:2016-02-03 00:00:00
abstract::We examined potential risk factors on vaccine virus shedding and antibody seroresponse to human rotavirus vaccine (Rotarix) in Mexican infants. Two doses of Rotarix were administered to infants during the first two visits for their routine childhood immunization (∼8 and 15weeks of age) in Mexico City. Infant's charact...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.09.006
更新日期:2016-10-17 00:00:00
abstract::A novel triterpenoidal saponin, called pulcherrimasaponin (CP05), isolated from the leaves of Calliandra pulcherrima Benth. shows remarkable similarities to the previously described potent adjuvant, QS21 saponin (Quillaja saponaria Molina). On the basis of chemical and physicochemical evidence, its structure was estab...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.08.022
更新日期:2005-01-11 00:00:00
abstract::The present work was carried out to develop an effective vaccine to control infectious bursal disease (IBD). The very virulent infectious bursal disease virus (vvIBDV) was adapted to grow in Vero-cell line after three serial passages. The virus was then attenuated by further serial passages and pathogenicity of differ...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/j.vaccine.2006.01.044
更新日期:2006-04-05 00:00:00
abstract:CONTEXT:Dubious vaccination-related information on the Internet leads some parents to opt out of vaccinating their children. OBJECTIVES:To determine if negative, neutral and positive search terms retrieve vaccination information that differs in valence and confirms searchers' assumptions about vaccination. METHODS:A ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.08.042
更新日期:2014-10-07 00:00:00
abstract::Cytology-based screening has reduced cervical cancer mortality in countries able to implement, sustain and financially support organized programs that achieve broad coverage. These ongoing secondary prevention efforts considerably complicate the question of whether vaccination against human papillomavirus (HPV) types ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2008.06.009
更新日期:2008-08-19 00:00:00
abstract::A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with beta-propiolactone. In ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.06.014
更新日期:2004-12-09 00:00:00
abstract::A transplantable hepatocarcinoma of strain 2 guinea pigs was used as an experimental model for immunotherapy of cancer. 6,6'-Dideoxy-6,6'-bis-mycoloylamino-alpha,alpha- trehalose (TDNM) was found to be more effective in producing regression of transplantable line-10 tumours than 6,6'-di-O-mycoloyl-alpha,alpha-trehalos...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(88)90146-6
更新日期:1988-10-01 00:00:00
abstract:INTRODUCTION:Older adults living in nursing homes (NH) are at high risk of developing influenza and pneumococcal infections. The objectives of this study were to describe vaccination coverage for influenza and pneumococcal among French NH residents and to investigate which NH structure- and organisation-related aspects...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.12.023
更新日期:2014-02-07 00:00:00
abstract::Since the mid-1970s, the widespread establishment and implementation of the Expanded Programme on Immunization (EPI) has led to remarkable achievements in controlling vaccine preventable diseases worldwide. Today, more children than ever are being reached with immunization; interruption of poliomyelitis transmission h...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2012.01.080
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:Patients with inflammatory rheumatic diseases are at higher risk for influenza and current guidelines recommend vaccination for this group of patients. The aim of this study was to evaluate the vaccination coverage and predictors for influenza vaccination among patients with inflammatory rheumatic diseases. ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.06.065
更新日期:2018-08-06 00:00:00
abstract::Actinobacillus pleuropneumoniae is a gram negative pleiomorphic rod that is the causative agent of a severe, highly infectious and often fatal pleuropneumonia in swine. We have previously reported the construction of genetically-defined stable riboflavin auxotrophs by replacement of a portion of the APP riboflavin bio...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(00)00076-1
更新日期:2000-06-15 00:00:00
abstract:BACKGROUND:Following a recommendation by the World Health Organization, Madagascar introduced rotavirus vaccine in 2014. Though national rotavirus vaccine coverage has remained <80%, rotavirus hospitalizations declined by 78%. Gavi, the Vaccine Alliance, has provided financial support for rotavirus vaccine, however the...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.09.082
更新日期:2020-11-03 00:00:00