Abstract:
:Effects of Rapamycin on the proliferation and apoptosis of retinoblastoma cells through the phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) signaling pathway were studied. The retinoblastoma Y79 cells were selected and divided into negative control group (NC group), 0.2 µM Rapamycin group and 0.4 µM Rapamycin group. Then the proliferative activity of Y79 cells was detected using Cell Counting Kit-8 (CCK8) assay, the content of reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) in cells in each group was detected using enzyme-linked immunosorbent assay (ELISA), and the apoptosis of Y79 cells was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Moreover, the changes in Y79 cell cycle and apoptosis were determined through flow cytometry, and apoptosis and PI3K/AKT pathway were detected using reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. It was found that the number of cells and the proliferative activity were significantly reduced in 0.2 µM Rapamycin group and 0.4 µM Rapamycin group. In 0.2 µM Rapamycin group and 0.4 µM Rapamycin group, the content of ROS and MDA was significantly decreased, while that of SOD was notably increased. TUNEL assay and flow cytometry showed that in 0.2 µM Rapamycin group and 0.4 µM Rapamycin group, the number of apoptotic cells was obviously increased, and the cell cycle was basically arrested in S phase. The expression levels of Bcl-2, PI3K and AKT declined in 0.2 µM Rapamycin group and 0.4 µM Rapamycin group, whereas the expression of Caspase 8 increased. Similar results were also obtained in the protein assay. The above results were significantly superior in 0.4 µM Rapamycin group to those in 0.2 µM Rapamycin group. Rapamycin inhibits proliferation and promotes apoptosis of retinoblastoma cells through inhibiting the PI3K/AKT signaling pathway.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Yao J,Xu M,Liu Zdoi
10.3892/ol.2020.11363subject
Has Abstractpub_date
2020-04-01 00:00:00pages
2950-2956issue
4eissn
1792-1074issn
1792-1082pii
OL-0-0-11363journal_volume
19pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Gallbladder cancer (GBC) is a rare but highly aggressive cancer for which no well-accepted prognostic biomarkers have been identified. Thymus cell antigen 1 (Thy1), also known as cluster of differentiation (CD)90, and integrin α6 (ITGA6), also known as CD49f, are important molecules in cancer and putative markers of v...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5341
更新日期:2016-12-01 00:00:00
abstract::Local adjuvant treatment of giant cell tumours (GCTs) of the bone with phenol has led to a significant reduction in recurrence rates. In the current study, the optimal phenol concentration and duration of intralesional exposure were evaluated. Specimens of GCTs were exposed to various concentrations of phenol solution...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1244
更新日期:2013-05-01 00:00:00
abstract::The etiology of laryngeal squamous cell carcinoma (LSCC) has not yet been adequately examined. Therefore, the present study aimed to investigate the association between serum folate deficiency and abnormal expression of the cell adhesion molecule 1 (CADM1) protein in the progression of LSCC. Samples were collected fro...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5264
更新日期:2016-12-01 00:00:00
abstract::The present study aimed to investigate the feasibility of using ecto-mesenchymal stem cell (EMSC)-derived dendritic cells (DCs) for glioma immunotherapy following infection by a recombinant adenovirus containing the melanoma-associated antigen D4a (MAGE-D4a) gene. The ex vivo cultured EMSCs were infected by the adenov...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4306
更新日期:2016-04-01 00:00:00
abstract::The aim of this study was to evaluate the efficacy of multi-detector row helical computed tomography (MDCT) angiography in the detection of feeding arteries prior to multi-arterial infusion for lung cancer. A total of 59 consecutive patients (44 males and 15 females; age range, 27-86 years; median age, 62 years) with ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1000
更新日期:2013-01-01 00:00:00
abstract::The putative oncogenic role of ATP/GTP binding protein like 2 (AGBL2) in catalyzing α-tubulin detyrosination has recently been characterized in cancer. However, the status of AGBL2 expression in ovarian cancer and its potential clinical and prognostic significance remain unclear. In the present study, immunohistochemi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10829
更新日期:2019-11-01 00:00:00
abstract::Pendred syndrome is a rare genetic disease that causes a disturbance in thyroid hormone synthesis, which results in thyroid dysfunction and the development of goiter and sensorineural deafness. The present report describes the case of a young euthyroid male, who developed a large goiter and hearing impairment, despite...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2461
更新日期:2014-11-01 00:00:00
abstract::Spontaneous remission (SR) in acute lymphoblastic leukemia (ALL) is a rare phenomenon, but the disease course and its underlying processes are of basic and clinical interest. Herein is reported the case of a pregnant, 31-year-old patient who developed ALL, followed by septic shock and SR of ALL. Information is summari...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7288
更新日期:2018-01-01 00:00:00
abstract::Glutathione S-transferases (GSTs) participate in the detoxification and elimination of electrophilic carcinogens by conjugating them to glutathione. Previous studies have reported a potential association between GSTM1 polymorphism and the risk of developing nasopharyngeal cancer (NPC). However, those findings remain c...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3848
更新日期:2016-01-01 00:00:00
abstract::In previous studies, we observed that STAT1 and Survivin correlated negatively with gastric cancer tissues, and that the functions of the IFN-γ-STAT1 pathway and Survivin in gastric cancer are the same as those reported for other types of cancer. In this study, the SGC7901 gastric cancer cell line and 83 gastric cance...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.423
更新日期:2012-01-01 00:00:00
abstract::Extraventricular choroid plexus papillomas (CPPs) are rare. In this study, we reveal the imaging findings of CPPs located in extraventricular sites. The imaging findings of 11 masses [10 masses on magnetic resonance imaging (MRI) and one mass on computed tomography (CT)] of extraventricular CPP in 10 patients were ret...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5552
更新日期:2017-03-01 00:00:00
abstract::Ascorbic acid (A) has been demonstrated to exhibit anti-cancer activity in association with chemotherapeutic agents. Potassium (K) is a regulator of cellular proliferation. In the present study, the biological effects of A and K bicarbonate, alone or in combination (A+K), on breast cancer cell lines were evaluated. Th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4506
更新日期:2016-06-01 00:00:00
abstract::A promising nutraceutical, apigenin, was recently revealed to exhibit biological activity in inhibiting several types of cancer. The effects of apigenin on the growth inhibition and apoptosis of the cholangiocarcinoma HuCCA-1 cell line were investigated. Protein alterations subsequent to apigenin treatment were studie...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6705
更新日期:2017-10-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) promotes invasive and migratory activities by upregulating Forkhead box protein M1 (FoxM1) expression. The present study investigated whether modulation of MnSOD and FoxM1 expression was responsible for the antitumor effects of genistein on cancer stem-like cells (CSLCs) derived ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11802
更新日期:2020-09-01 00:00:00
abstract::Voltage-dependent anion channels (VDACs) are situated in the outer membrane of the mitochondria and serve as gatekeepers that control metabolite and ion exchange between the cytosol and mitochondria. VDAC1 is one of the most studied members of the VDAC protein family and is overexpressed in multiple types of cancer. H...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10534
更新日期:2019-09-01 00:00:00
abstract::Observational and experimental studies have produced inconsistent evidence about the association of serum levels of B-type natriuretic peptide (BNP) with anthracycline-induced cardiotoxicity (AIC). Therefore, the current meta-analysis examined the association between serum BNP levels and AIC by using data from high qu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4424
更新日期:2016-05-01 00:00:00
abstract::C-X-C chemokine receptor types 1/2 (CXCR1/2) may play multiple roles in the development and progression of a number of types of tumor. The abnormal expression of CXCR1/2 in various types of malignant tumors has been reported, but less is known with regard to gastric carcinoma. The present study was preliminarily condu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1043
更新日期:2013-02-01 00:00:00
abstract::Resistance to apoptosis is an characteristic of cancer cells that serves a critical function in tumor development and represents a target for antitumor therapy. Isoimperatorin (ISOIM), a coumarin compound, exhibits antitumor functions in multiple types of tumor cells. However, its antitumor effects and molecular mecha...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8303
更新日期:2018-05-01 00:00:00
abstract::Cervical cancer is the third most common malignancy worldwide, accounting for 250,000 mortalities annually. Notch1, an important regulator of cell-fate decisions and differentiation, has been found to be overexpressed in certain types of cancer. However, the role of Notch1 in cervical carcinogenesis remains unclear. I...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3143
更新日期:2015-06-01 00:00:00
abstract::For individualized bevacizumab-based therapy, non-invasive biomarkers are necessary. This study assessed the predictive value of plasma vascular endothelial growth factor (VEGF)-A, soluble VEGF receptor (sVEGFR)-1 and sVEGFR-2 levels as biomarkers for clinical response and survival in advanced colorectal cancer (CRC) ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000045
更新日期:2010-03-01 00:00:00
abstract::Lung cancer is one of the most common cancers, which is the leading cause of cancer-related death among various cancers worldwide. Flavokawain A (FKA), a chalcone found in the kava plant, exerts potent anticancer activity. However, the activity and mechanisms of FKA in inhibiting the viability of paclitaxel (PTX)-resi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11069
更新日期:2020-01-01 00:00:00
abstract::Death associated protein kinase 1 (DAPK1) is a notable serine/threonine kinase involved in the regulation of multiple cellular pathways, including apoptosis and autophagy. Although DAPK1 is usually considered to be a tumor suppressor, it was previously reported to promote the viability of p53 mutant cancer cell lines ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8439
更新日期:2018-06-01 00:00:00
abstract::Chylothorax is the accumulation of lymph fluid in the pleura. Gastric carcinoma with chylothorax and lymphedema as the initial manifestations has rarely been reported, with only 14 cases reported to date. The mechanisms of gastric carcinoma generating chylothorax have not yet been determined. The current study reports...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4285
更新日期:2016-04-01 00:00:00
abstract::An individual with a twin who has developed leukemia or non-Hodgkin lymphoma (NHL) has an increased risk of developing the same disease, particularly with monozygotic twins. The few reported pairs of twins who developed NHL had similar primary sites and pathological subtypes. Here, we present the first reported cases ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9723
更新日期:2019-01-01 00:00:00
abstract::A 20-year-old male presented to hospital with blood in the mucus, which had occurred for 6 months, and was diagnosed with nasopharyngeal carcinoma (NPC). Due to passive smoking, the patient developed a constant and violent cough, pain behind the right ear and swelling of the retropharynx. The results of a computed tom...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4473
更新日期:2016-06-01 00:00:00
abstract::Primary hemangioendotheliomas (HEs) of the spleen are rare, low-grade borderline-malignant vascular tumors. To date, only a few splenic HE cases have been reported in adults. In infants, one 9-year-old male patient has previously been reported, and the patient succumbed to the disease shortly following surgery. Curren...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2693
更新日期:2015-01-01 00:00:00
abstract::The present study aimed to investigate the clinical significance of paired-box 8 (PAX8) in primary epithelial ovarian cancer (PEOC). Using immunohistochemical (IHC) staining, the expression of PAX8 in 60 patients with PEOC, 20 patients with ovarian benign lesions and 10 patients with metastatic ovarian cancer (MOC), w...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6949
更新日期:2017-11-01 00:00:00
abstract::The aim of the present study was to investigate whether an increase in cyclin-dependent kinase 2 (CDK2) activity is involved in apoptosis of human bladder cancer T24 cells induced by isoliquiritigenin (ISL). The viability of T24 cells was estimated using a sulforhodamine B assay. Cell morphological changes were examin...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6159
更新日期:2017-07-01 00:00:00
abstract::We investigated the possible association between DLKI gene promoter region methylation and the increased invasion capacity of non-small cell lung cancer (NSCLC). Lung cancer cell line H1299, as well as the gene transfection and RNA interference technology were used to build DLK gene overexpression and knockdown cells....
journal_title:Oncology letters
pub_type: 杂志文章,收录出版
doi:10.3892/ol.2017.6019
更新日期:2017-06-01 00:00:00
abstract::The aim of the present study was to develop a water-soluble biomarker for the detection of breast cancer using quantum dots (QDs) conjugated to Ki-67, a nuclear protein associated with the cell cycle. Ki-67 is also a marker of cell proliferation, with expression levels categorizing good and poor prognosis in invasive ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7615
更新日期:2018-02-01 00:00:00