Abstract:
:The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
journal_name
Sci Advjournal_title
Science advancesauthors
Carter SD,Hampton CM,Langlois R,Melero R,Farino ZJ,Calderon MJ,Li W,Wallace CT,Tran NH,Grassucci RA,Siegmund SE,Pemberton J,Morgenstern TJ,Eisenman L,Aguilar JI,Greenberg NL,Levy ES,Yi E,Mitchell WG,Rice WJ,Wiggedoi
10.1126/sciadv.aay9572subject
Has Abstractpub_date
2020-04-01 00:00:00pages
eaay9572issue
14issn
2375-2548pii
aay9572journal_volume
6pub_type
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