Abstract:
:Numerous infectious diseases impacting livestock impose an important economic burden and in some cases also represent a threat to humans and are classified as zoonoses. Some zoonotic diseases are transmitted by vectors and, due to complex environmental and socio-economic factors, the distribution of many of these pathogens is changing, with increasing numbers being found in previously unaffected countries. Here, we developed a multiplex assay, based on a suspension microarray, able to detect specific antibodies to five important pathogens of livestock (three of them zoonotic) that are currently emerging in new geographical locations: Rift Valley fever virus (RVFV), Crimean-Congo haemorrhagic fever virus (CCHFV), Schmallenberg virus (SBV), Bluetongue virus (BTV) and the bacteria complex Mycobacterium tuberculosis. Using the Luminex platform, polystyrene microspheres were coated with recombinant proteins from each of the five pathogens. The mix of microspheres was used for the simultaneous detection of antibodies against the five corresponding diseases affecting ruminants. The following panel of sera was included in the study: 50 sera from sheep experimentally infected with RVFV, 74 sera from calves and lambs vaccinated with SBV, 26 sera from cattle vaccinated with Mycobacterium bovis, 30 field sera from different species of ruminants infected with CCHFV and 88 calf sera infected with BTV. Finally, to determine its diagnostic specificity 220 field sera from Spanish farms free of the five diseases were assessed. All the sera were classified using commercial ELISAs specific for each disease, used in this study as the reference technique. The results showed the multiplex assay exhibited good performance characteristics with values of sensitivity ranging from 93% to 100% and of specificity ranging from 96% to 99% depending on the pathogen. This new tool allows the simultaneous detection of antibodies against five important pathogens, reducing the volume of sample needed and the time of analysis where these pathogens are usually tested individually.
journal_name
Transbound Emerg Disjournal_title
Transboundary and emerging diseasesauthors
Hoste ACR,Ruiz T,Fernández-Pacheco P,Jiménez-Clavero MÁ,Djadjovski I,Moreno S,Brun A,Edwards TA,Barr JN,Rueda P,Sastre Pdoi
10.1111/tbed.13776subject
Has Abstractpub_date
2020-08-07 00:00:00eissn
1865-1674issn
1865-1682pub_type
杂志文章abstract::In 2011, a human brucellosis case with severe clinical symptoms was reported at the University Clinic for Infectious Diseases in Prishtina, Kosovo. A trace-back investigation was conducted to find the source of human infection. A total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised o...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12336
更新日期:2016-12-01 00:00:00
abstract::Staphylococcus aureus is a versatile and highly adaptable pathogen associated with a wide range of infectious diseases in humans and animals. In the last decades, concern has increased worldwide due to the emergence and spread of methicillin-resistant S. aureus (MRSA) strains shortly after this drug became a therapeut...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13771
更新日期:2020-08-06 00:00:00
abstract::Armadillos are specialist diggers and their burrows are used to find food, seek shelter and protect their pups. These burrows can also be shared with dozens of vertebrate and invertebrate species and; consequently, their parasites including the zoonotics. The aim of this study was to diagnose the presence of zoonotic ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13839
更新日期:2020-09-22 00:00:00
abstract::The aim of our study was to determine species and genotypes of Cryptosporidium in patients suffering from immunosuppressive illnesses, but also in immunocompetent patients suffering from diarrhoea. A total of 80 samples of faeces were collected from both immunosuppressed and immunocompetent patients. The immunosuppres...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13009
更新日期:2019-01-01 00:00:00
abstract::Hepatitis E is a significant liver disease caused by infection with hepatitis E virus (HEV). The risk factors for hepatitis E in developed countries include blood transfusion and ingestion of undercooked meat or meat products derived from HEV-infected animals. Since 2000, there has been increased human hepatitis E inc...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13977
更新日期:2021-01-06 00:00:00
abstract::Bluetongue (BT), caused by bluetongue virus (BTV), is a vector-borne disease of small ruminants that has the potential to spread across international borders. Despite large populations of susceptible animals and borders with BTV endemic countries, little is known of the disease burden and prevalent serotypes in the pr...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12871
更新日期:2018-10-01 00:00:00
abstract::Pre-clinical responses to fast-moving infectious disease outbreaks heavily depend on choosing the best isolates for animal models that inform diagnostics, vaccines and treatments. Current approaches are driven by practical considerations (e.g. first available virus isolate) rather than a detailed analysis of the chara...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13588
更新日期:2020-07-01 00:00:00
abstract::Rift Valley fever (RVF) is a vector-borne zoonotic disease that has caused widespread outbreaks throughout Africa and the Arabian Peninsula, with serious consequences for livestock-based economies and public health. Although there have never been any reports of RVF in Morocco, Algeria, Tunisia and Libya, it is a prior...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12331
更新日期:2016-12-01 00:00:00
abstract::In 2014, a new bluetongue virus serotype 4 (BTV-4) strain was detected in southern Greece and spread rapidly throughout the Balkan Peninsula and adjacent countries. Within half a year, more than 7,068 outbreaks were reported in ruminants, particularly in sheep. However, the reported morbidity and case fatality rates i...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12746
更新日期:2018-04-01 00:00:00
abstract::Mycobacterium bovis is the causal agent of bovine tuberculosis (BTB), with a diverse host range, extending from livestock to domestic and captive wild animals as well as free-ranging wildlife species. In South Africa, BTB is endemic in the Kruger National Park (KNP) and the Hluluwe iMfolozi National Park (HiP), where ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12132
更新日期:2013-11-01 00:00:00
abstract::Wild African Suidae, the common warthog (Phacochoerus africanus) and bushpig (Potamochoerus larvatus), were experimentally infected with classical swine fever (CSF) virus following the diagnosis of CSF subtype 2.1 in domestic pigs in South Africa in 2005. No data regarding the susceptibility or potential lesions of th...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2010.01191.x
更新日期:2011-04-01 00:00:00
abstract::Although vaccination is the main measure to control influenza A virus (IAV) in swine, there is limited information on the efficacy of sow vaccination on reducing IAV infections in pigs at weaning. We assessed the effect of sow vaccination on IAV infection in pigs at weaning in a cohort of 52 breeding herds studied pro...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13688
更新日期:2020-07-11 00:00:00
abstract::Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides-borne arbovirus, the causative agent of bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind-blown dissemin...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13156
更新日期:2019-05-01 00:00:00
abstract::A total of 1279 Eurasian wild boar (Sus scrofa) sera were collected from 2000 to 2011 in the Iberian Peninsula to reveal time changes in serum antibody prevalences against selected infectious agents (porcine circovirus type 2, PCV2; porcine reproductive and respiratory syndrome virus, PRRSV; hepatitis E virus, HEV; an...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2011.01285.x
更新日期:2012-10-01 00:00:00
abstract::Mycoplasma suis and Mycoplasma parvum bind strongly to erythrocytes and may cause clinical hemoplasmosis in swine, affecting several age groups. Mycoplasma spp. infected animals may be asymptomatic carriers and/or show nonspecific clinical signs. In Brazil, information on genetic diversity associated with porcine hemo...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13767
更新日期:2020-08-04 00:00:00
abstract::The study evaluated the effectiveness of 'Mass Vaccination Campaign (MVC)' implemented against the contagious transboundary OIE notified Peste des petits ruminants (PPR) in sheep and goats on the lines of 'pulse polio campaign' for humans in Chhattisgarh state, India. The effectiveness was evaluated on the axes of ade...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13163
更新日期:2019-05-01 00:00:00
abstract::Foot and mouth disease (FMD) is a highly contagious disease of ruminants that causes huge economic losses around the globe. However, the prevalence of FMDV in small ruminants has been overlooked in Pakistan. A seroepidemiological study was conducted in Chakwal, Faisalabad and Khanewal districts of Punjab, Pakistan to ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12194
更新日期:2014-08-01 00:00:00
abstract::An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the i...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13544
更新日期:2020-03-12 00:00:00
abstract::Since the first report of bovine digital dermatitis (DD) in 1974, there is a large body of the literature published; however, effective prevention and control of the disease remain elusive. Although many aspects of the pathogenesis of DD have been investigated, even some of the most basic questions such as the aetiolo...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.12729
更新日期:2018-05-01 00:00:00
abstract::The role and importance of pigs and pork as sources of zoonotic hepatitis E virus (HEV) has been debated in Canada and abroad for over 20 years. To further investigate this question, we compiled data to populate a risk profile for HEV in pigs or pork in Canada. We organized the risk profile (RP) using the headings pre...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.12582
更新日期:2017-12-01 00:00:00
abstract::European starlings (Sturnus vulgaris), house sparrows (Passer domesticus) and rock pigeons (Columba livia) are all wild birds commonly found in large numbers in and around human dwellings and domestic livestock operations. This study evaluated the susceptibility of these species to three strains of highly pathogenic a...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13147
更新日期:2019-05-01 00:00:00
abstract::Ducks are the "Trojan Horses" for Asian H5N1 avian influenza viruses (AIV) and attain carrier status without displaying overt infection. These birds help in the spread of the virus among the poultry and human population through direct or indirect contact. Preen oil is the secretion of preen gland of water birds such a...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13148
更新日期:2019-05-01 00:00:00
abstract::Since African swine fever virus (ASFV) introduction into Vietnam in 2019, most ASFV strains detected in this country belong to the p72 genotype II and intergenic region (IGR) II variant. Further investigation of the intergenic region of ASFVs isolated in the Capital Hanoi region showed two different variants, IGR I an...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13996
更新日期:2021-01-20 00:00:00
abstract::Phylogenetic studies on foot-and-mouth disease viruses (FMDVs) circulating in the West Eurasian region have largely focused on the genomic sequences encoding the structural proteins that determine the serotype. The present study has compared near-complete genome sequences of FMDVs representative of the viruses that ci...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13433
更新日期:2020-03-01 00:00:00
abstract::Non-tuberculous mycobacteria (NTM) are widely distributed in the environment, particularly in wet soil, marshland, rivers or streams, but also are causative agents of a wide variety of infections in animals and humans. Little information is available regarding the NTM prevalence in wildlife and their effects or signif...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12083
更新日期:2015-02-01 00:00:00
abstract::In previous studies, Theileria annulata surface protein (TaSP) was identified as an immunodominant antigen and successfully used to develop and validate a recombinant-protein-based ELISA for the detection of circulating antibodies in serum of T. annulata-infected animals. In this study, the same antigen was used to de...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2008.01036.x
更新日期:2008-08-01 00:00:00
abstract::This literature review provides an overview of use of environmental samples (ES) such as faeces, water, air, mud and swabs of surfaces in avian influenza (AI) surveillance programs, focussing on effectiveness, advantages and gaps in knowledge. ES have been used effectively for AI surveillance since the 1970s. Results ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.13633
更新日期:2020-07-11 00:00:00
abstract::Swine enteric coronaviruses, including porcine epidemic diarrhoea virus (PEDV) and porcine deltacoronavirus (PDCoV), have emerged and spread throughout the North American swine industry over the last four years. These diseases cause significant losses within the pork industry and within the first year after PEDV intro...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12823
更新日期:2018-06-01 00:00:00
abstract::Since African swine fever (ASF) first appeared in the Caucasus region in 2007, it has spread rapidly and is now present in numerous European and Asian countries. In Europe, mainly wild boar populations are affected and pose a risk for domestic pigs. In Asia, domestic pigs are almost exclusively affected. An effective ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13678
更新日期:2020-11-01 00:00:00
abstract::Senecavirus A (SVA) is the only member of genus Senecavirus that causes vesicular lesions in pigs. We have characterized seven SVA isolates from different swine farms in Guangdong, China. The most variable isolate, CH-DL-01-2016, contained a single amino acid insertion at position 219-220 and a 16 amino acid insertion...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12619
更新日期:2017-12-01 00:00:00