Abstract:
:Mycoplasma suis and Mycoplasma parvum bind strongly to erythrocytes and may cause clinical hemoplasmosis in swine, affecting several age groups. Mycoplasma spp. infected animals may be asymptomatic carriers and/or show nonspecific clinical signs. In Brazil, information on genetic diversity associated with porcine hemoplasmas (PH) has not been described yet. Therefore, this study has aimed to detect, quantify and characterize the genetic diversity of PH in finishing pigs from technified farms in the state of Goiás, central-western Brazil. Ethylenediaminetetraacetic acid-blood samples from 450 swine belonging to 30 different farms from Goiás state were collected at the slaughterhouse. Quantitative real-time PCR (qPCR) assays were performed for the molecular detection and quantification of PH 16S rRNA gene fragments. Cloning and sequencing of 16S and 23S rRNA amplicons were performed to evaluate the genetic diversity. Moreover, a questionnaire was applied to each farm manager to obtain epidemiological information about the herd. The results on qPCR showed herd occurrence of 68.89% for PH. Quantification values (starting quantity [SQ]) ranged from 8.43 × 10-1 to 4.69 × 106 copies/µl, and 52.71% of the samples presented SQ values equal or lower than 1 × 103 copies/µl. Risk factors were not evaluated once all farms had at least one positive animal. However, Spearman's coefficient test revealed that the occurrence of PH was inversely associated with the number of farrows per week, weaned piglets per week, and weight at slaughter. Phylogenetic analysis based on maximum likelihood and Bayesian methods showed that the 16S rRNA and 23S rRNA gene sequences obtained from five samples formed a single cluster closely related to M. parvum. Genotype analysis using DNASP software confirmed seven and four different 16S and 23S rRNA genotypes among the cloned amplicons, indicating that there are several genotypes of M. parvum circulating in individual pigs and among pig farms in central-western Brazil.
journal_name
Transbound Emerg Disjournal_title
Transboundary and emerging diseasesauthors
Sonalio K,Perles L,Gatto IRH,do Amaral RB,Almeida HMS,Galdeano JVB,Vieira RFC,André MR,de Oliveira LGdoi
10.1111/tbed.13767subject
Has Abstractpub_date
2020-08-04 00:00:00eissn
1865-1674issn
1865-1682pub_type
杂志文章abstract::Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection in cattle has been largely correlated with the FMD virus (FMDV)-specific antibodies. Genetic control of cattle immune adaptive responses has...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12130
更新日期:2015-06-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is endemic in China and is predominantly due to foot-and-mouth disease virus (FMDV) serotype O Mya-98 lineage. In recent years, FMDV O/ME-SA/Ind2001 lineage has spread from the Indian subcontinent to South-East Asia, Middle East and Africa, which may pose potential threats for future trans...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12895
更新日期:2018-12-01 00:00:00
abstract::Schmallenberg virus (SBV) infections can cause congenital musculoskeletal and vertebral malformations as well as neurological failures in foetuses of several ruminant species if susceptible mother animals were infected during early gestation. Blood samples gained from 17 goat and 64 sheep flocks in Lower Saxony (LS), ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12161
更新日期:2015-08-01 00:00:00
abstract::The raw case fatality rate (CFR, the reported number of COVID-19 deaths divided by the total number of cases) is an important indicator to quantify the severity or treatment efficacy. In many countries, the pandemic had experienced two waves to date. To our knowledge, no studies have compared the CFR between the two w...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13819
更新日期:2020-09-06 00:00:00
abstract::Carbapenem-resistant Enterobacteriaceae (CRE) have been rapidly increasing among animals in many countries and have been a great threat to public health. Horse riding is becoming increasingly popular worldwide; however, reports of CRE producing NDM or KPC-2, two prevalent types of carbapenemases, from horses of equest...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13614
更新日期:2020-05-09 00:00:00
abstract::Wildlife and notably deer species--due to the increasing relevance of deer farming worldwide--may contribute to the maintenance of Coxiella burnetii, the causal agent of Q fever. Currently, there are no precedents linking exposure to deer species with human Q fever cases. However, a human case of Q fever was recently ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12179
更新日期:2015-10-01 00:00:00
abstract::We use swine shipping data from Manitoba to construct one-mode dynamic contact networks of swine locations and two-mode location-to-truck networks at four time scales: daily, weekly, monthly and for the entire two-year study period. We provide measures of graph evolution and graph characterization for each, useful in ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13220
更新日期:2019-09-01 00:00:00
abstract::Mycobacterium bovis is the causal agent of bovine tuberculosis (BTB), with a diverse host range, extending from livestock to domestic and captive wild animals as well as free-ranging wildlife species. In South Africa, BTB is endemic in the Kruger National Park (KNP) and the Hluluwe iMfolozi National Park (HiP), where ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12132
更新日期:2013-11-01 00:00:00
abstract::Sloths are genetically and physiologically divergent mammals. Phleboviruses are major arthropod-borne viruses (arboviruses) causing disease in humans and other animals globally. Sloths host arboviruses, but virus detections are scarce. A phlebovirus termed Anhanga virus (ANHV) was isolated from a Brazilian Linnaeus's ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13333
更新日期:2020-01-01 00:00:00
abstract::In recent decades, cases of autochthonous hepatitis E (HE) have sharply increased in European countries where foodborne transmission is considered the main route of HE virus (HEV) transmission. Although rabbits are considered the main reservoir of the zoonotic HEV-3ra subtype, information on the role of wild lagomorph...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13478
更新日期:2020-07-01 00:00:00
abstract::The aim of this study was to better understand the molecular epidemiology of Campylobacter coli isolated from multiple sources in Belgium, by studying the genotypic diversity and antimicrobial resistance phenotypes and resistance mechanisms of 59 C. coli isolates. Isolates from broiler carcasses and human cases were g...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13046
更新日期:2019-01-01 00:00:00
abstract::European starlings (Sturnus vulgaris), house sparrows (Passer domesticus) and rock pigeons (Columba livia) are all wild birds commonly found in large numbers in and around human dwellings and domestic livestock operations. This study evaluated the susceptibility of these species to three strains of highly pathogenic a...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13147
更新日期:2019-05-01 00:00:00
abstract::The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen sample...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13545
更新日期:2020-03-11 00:00:00
abstract::An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in mo...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12062
更新日期:2014-12-01 00:00:00
abstract::Small- and medium-sized mammals play an important role in the life cycle of tick-borne pathogens in urban habitats. Our aim was to apply the general protocol, DAMA (documentation-assessment-monitoring-action), which is an integrated proposal to build a proactive capacity to understand, anticipate, and respond to the o...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13019
更新日期:2019-01-01 00:00:00
abstract::Brucella melitensis is a serious public health threat, with human infection exhibiting acute febrile illness and chronic health problems. The present study investigated the genetic diversity and epidemiological links of the important zoonotic bacterium B. melitensis in Egypt using multilocus variable-number tandem rep...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13581
更新日期:2020-11-01 00:00:00
abstract::Leishmaniosis is a tropical and subtropical vector-borne disease caused by hemoparasites of the genus Leishmania. The disease can infect humans, as well as domestic and wildlife animals. Dogs are the main reservoir for L. infantum, the aetiological agent of visceral leishmaniosis (VL) in America, and a domestic source...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13313
更新日期:2020-07-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that is caused by the FMD virus (FMDV). This disease affects both wild and domestic cloven-hoofed animals, and the latter of which includes cattle, swine, sheep and goats. FMD is endemic to Jordan and has a severe impact on the productivity of domes...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13372
更新日期:2020-01-01 00:00:00
abstract::In this study, the pathogenicity of porcine deltacoronavirus (PDCoV) strain NH (passage 10, P10) was evaluated. We found that PDCoV strain NH is enteropathogenic in 5-day-old pigs. Pathogenicity experiments provided a challenge model for studying the protection efficiency of passive immunity. In order to investigate t...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13369
更新日期:2020-03-01 00:00:00
abstract::Rift Valley fever virus (RVFV) causes consistently severe outbreaks with high public health impacts and economic losses in livestock in many African countries and has also been introduced to Saudi Arabia and Yemen. Egypt with its four large outbreaks in the last 40 years represents the northernmost endemic area of RVF...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12616
更新日期:2017-12-01 00:00:00
abstract::Poultry can become infected with avian influenza viruses (AIV) via (in) direct contact with infected wild birds. Free-range chicken farms in the Netherlands were shown to have a higher risk for introduction of low pathogenic avian influenza (LPAI) virus than indoor chicken farms. Therefore, during outbreaks of highly ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13649
更新日期:2020-06-07 00:00:00
abstract::Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides-borne arbovirus, the causative agent of bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind-blown dissemin...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13156
更新日期:2019-05-01 00:00:00
abstract::The role and importance of pigs and pork as sources of zoonotic hepatitis E virus (HEV) has been debated in Canada and abroad for over 20 years. To further investigate this question, we compiled data to populate a risk profile for HEV in pigs or pork in Canada. We organized the risk profile (RP) using the headings pre...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.12582
更新日期:2017-12-01 00:00:00
abstract::Senecavirus A (SVA) is the only member of genus Senecavirus that causes vesicular lesions in pigs. We have characterized seven SVA isolates from different swine farms in Guangdong, China. The most variable isolate, CH-DL-01-2016, contained a single amino acid insertion at position 219-220 and a 16 amino acid insertion...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12619
更新日期:2017-12-01 00:00:00
abstract::Using a hospital-based case-control study design, our aim was to identify risk factors for-and space-time clusters of-Peste des Petits Ruminants (PPR) in Mymensingh, Bangladesh. Three hundred and eighty PPR cases diagnosed between January 2005 and December 2014 at the Bangladesh Agricultural University Veterinary Teac...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12615
更新日期:2017-12-01 00:00:00
abstract::In 2011, a human brucellosis case with severe clinical symptoms was reported at the University Clinic for Infectious Diseases in Prishtina, Kosovo. A trace-back investigation was conducted to find the source of human infection. A total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised o...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12336
更新日期:2016-12-01 00:00:00
abstract::Fourteen European Union (EU) countries have been affected by highly pathogenic avian influenza (HPAI) of the subtype H5N1 since 2005. Many of the H5N1 HPAI cases have been identified in wild bird populations from aquatic habitats. The objective of this contribution was to assess the association between the H5N1 HPAI i...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2008.01046.x
更新日期:2008-12-01 00:00:00
abstract::Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Madagascar and the Middle East. The aim of this study was to investigate the potential role of ixodid (hard) ticks in the transmission of the disease. Cattle ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2010.01184.x
更新日期:2011-04-01 00:00:00
abstract::Prevalence, distribution and antibiotic resistance of Arcobacter spp. were investigated in cattle, goats, floor and treated water samples in this study. The prevalence of Arcobacter in adult and young was recorded as 8/110 (7.27%) and 4/83 (4.81%), respectively, which showed insignificant difference (P = 0.3503) in de...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2012.01311.x
更新日期:2013-02-01 00:00:00
abstract::The objective of the present study was to access the titres and duration of maternally derived neutralizing antibodies against lumpy skin disease virus (LSDV) in calves born to immunized dairy cows. The study was conducted in a Greek farm of 200 Holstein cows which were immunized with a homologous Neethling strain-bas...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12983
更新日期:2018-12-01 00:00:00