Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization.

Abstract:

:Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides-borne arbovirus, the causative agent of bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind-blown dissemination of infected midges. Here, we report the first identification of BTV-3 in Sardinia, Italy. BTV-3 circulation was evidenced in sentinel animals located in the province of Sud Sardegna on September 19, 2018. Prototype strain BTV-3 SAR2018 was isolated on cell culture. BTV-3 SAR2018 sequence and partial sequences obtained by next-generation sequencing from nucleic acids purified from the isolate and blood samples, respectively, were demonstrated to be almost identical (99-100% of nucleotide identity) to BTV-3 TUN2016 identified in Tunisia in 2016 and 2017, a scenario already observed in past incursions of other BTV serotypes originating from Northern Africa.

journal_name

Transbound Emerg Dis

authors

Cappai S,Rolesu S,Loi F,Liciardi M,Leone A,Marcacci M,Teodori L,Mangone I,Sghaier S,Portanti O,Savini G,Lorusso A

doi

10.1111/tbed.13156

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

1426-1431

issue

3

eissn

1865-1674

issn

1865-1682

journal_volume

66

pub_type

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