Development of a competitive ELISA for detection of Theileria annulata infection.

Abstract:

:In previous studies, Theileria annulata surface protein (TaSP) was identified as an immunodominant antigen and successfully used to develop and validate a recombinant-protein-based ELISA for the detection of circulating antibodies in serum of T. annulata-infected animals. In this study, the same antigen was used to develop a competitive ELISA (cELISA) using a monoclonal antibody that was found to bind to TaSP. The cELISA accurately differentiated T. annulata-infected from uninfected animals and demonstrated a satisfactory performance with a calculated sensitivity and specificity of 77.4% and 100%, respectively. Thus the test proved its suitability for the diagnosis of tropical theileriosis and has application for use in serological surveys to monitor the prevalence of the disease or identify carrier animals with high specificity.

journal_name

Transbound Emerg Dis

authors

Renneker S,Kullmann B,Gerber S,Dobschanski J,Bakheit MA,Geysen D,Shiels B,Tait A,Ahmed JS,Seitzer U

doi

10.1111/j.1865-1682.2008.01036.x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

249-56

issue

5-6

eissn

1865-1674

issn

1865-1682

pii

JVA1036

journal_volume

55

pub_type

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