Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.

Abstract:

:Porcine respiratory disease complex (PRDC) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. No simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with PRDC. Furthermore, the detection of most of the bacterial pathogens implicated in PRDC currently requires time-consuming culture-based methods that can take several days to obtain results. In this study, a novel prototype automated microarray that integrates and automates all steps of post-PCR microarray processing for the simultaneous detection and typing of eight bacteria and viruses commonly associated with PRDC is described along with associated multiplex reverse transcriptase PCR. The user-friendly assay detected and differentiated between four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella enterica serovar Choleraesuis, Streptococcus suis), and further differentiated between type 1 and type 2 PRRSV as well as toxigenic and non-toxigenic P. multocida. The assay accurately identified and typed a panel of 34 strains representing the eight targeted pathogens and was negative when tested with 34 relevant and/or closely related non-target bacterial and viral species. All targets were also identified singly or in combination in a panel of clinical lung samples and/or experimentally inoculated biological material.

journal_name

Transbound Emerg Dis

authors

Lung O,Ohene-Adjei S,Buchanan C,Joseph T,King R,Erickson A,Detmer S,Ambagala A

doi

10.1111/tbed.12449

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

834-848

issue

3

eissn

1865-1674

issn

1865-1682

journal_volume

64

pub_type

杂志文章
  • Static and dynamic systems in Rickettsia slovaca life cycle evaluated by quantitative real-time polymerase chain reaction.

    abstract::Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2010.01104.x

    authors: Spitalská E,Sparagano O,Boldis V

    更新日期:2010-04-01 00:00:00

  • Rhodotorula minuta fungemia in a ewe lamb.

    abstract::An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in mo...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12062

    authors: Chitko-McKown CG,Leymaster KA,Heaton MP,Griffin DD,Veatch JK,Jones SA,Clawson ML

    更新日期:2014-12-01 00:00:00

  • Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand.

    abstract::Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Flavivirus genus and Flaviviridae family. The transmission of DTUMV involves mosquito vectors; however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. This study was conducted to examine DTMUV detecti...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13474

    authors: Sanisuriwong J,Yurayart N,Thontiravong A,Tiawsirisup S

    更新日期:2020-05-01 00:00:00

  • Isolation and Characterization of a Moderately Virulent Classical Swine Fever Virus Emerging in China.

    abstract::Classical swine fever (CSF) is a devastating infectious disease of pigs caused by classical swine fever virus (CSFV). In China, CSF has been under control owing to extensive vaccination with the lapinized attenuated vaccine (C-strain) since 1950s, despite sporadic or endemic in many regions. However, recently, CSF out...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12581

    authors: Luo Y,Ji S,Liu Y,Lei JL,Xia SL,Wang Y,Du ML,Shao L,Meng XY,Zhou M,Sun Y,Qiu HJ

    更新日期:2017-12-01 00:00:00

  • A molecular epidemiological investigation of PEDV in China: Characterization of co-infection and genetic diversity of S1-based genes.

    abstract::Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging epizootic virus of swine that causes substantial economic losses to the pig industry in China and other countries. The variations in the virus, and its co-infections with other enteric viruses, have contributed to the poor control of PEDV infection...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13439

    authors: Su M,Li C,Qi S,Yang D,Jiang N,Yin B,Guo D,Kong F,Yuan D,Feng L,Sun D

    更新日期:2020-05-01 00:00:00

  • Identification and molecular characterization of Staphylococcus aureus and multi-drug resistant MRSA from monkey faeces in China.

    abstract::Staphylococcus aureus is a commensal bacterium and an important opportunistic pathogen in humans and animals. The increase in multi-drug resistant (MDR) strains of S. aureus is a growing concern due to their impact on animal health and potential for zoonotic transmission. Increasing evidence has shown that MRSA could ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13450

    authors: Li Y,Tang Y,Ren J,Huang J,Li Q,Ingmer H,Jiao X

    更新日期:2020-05-01 00:00:00

  • Genetic characterization of parvoviruses in domestic cats in Henan province, China.

    abstract::Feline panleukopenia virus (FPV) and canine parvovirus (CPV) infections are highly contagious and cause serious enteric diseases, with high fatality rates of cats and dogs. Given the importance of cats as a potential source of genetic diversity for parvoviruses, parvovirus strains detected in cats with gastroenteritis...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13014

    authors: Li X,Wu H,Wang L,Spibey N,Liu C,Ding H,Liu W,Liu Y,Tian K

    更新日期:2018-12-01 00:00:00

  • Structuring the passive surveillance network improves epizootic detection and control efficacy: a simulation study on foot-and-mouth disease in France.

    abstract::Rapid detection of infection is critical to the containment and control of contagious pathogens. Passive surveillance, based on the detection of clinical signs through farmers' observations and subsequent veterinarian notification, is the primary means of initially detecting an epizootic and for implementing control m...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2011.01271.x

    authors: Rautureau S,Dufour B,Durand B

    更新日期:2012-08-01 00:00:00

  • Inter-serotype reassortment among epizootic haemorrhagic disease viruses in the United States.

    abstract::First described in 1955 in New Jersey, epizootic haemorrhagic disease (EHD) causes a severe clinical disease in wild and domestic ruminants worldwide. Epizootic haemorrhagic disease outbreaks occur in deer populations each year from summer to late autumn. The etiological agent is EHD virus (EHDV) which is a double-str...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13257

    authors: Wang L,Lanka S,Cassout D,Mateus-Pinilla NE,Li G,Wilson WC,Yoo D,Shelton P,Fredrickson RL

    更新日期:2019-09-01 00:00:00

  • The evolution of pigeon paramyxovirus type 1 (PPMV-1) in Great Britain: a molecular epidemiological study.

    abstract::Newcastle disease (ND), caused by virulent strains of avian paramyxovirus type 1 (APMV-1), is considered throughout the world as one of the most important animal diseases. For over three decades now, there has been a continuing panzootic caused by a variant virulent APMV-1 strain, so-called pigeon paramyxovirus type 1...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12006

    authors: Aldous EW,Fuller CM,Ridgeon JH,Irvine RM,Alexander DJ,Brown IH

    更新日期:2014-04-01 00:00:00

  • Experimental infection of common warthogs (Phacochoerus africanus) and bushpigs (Potamochoerus larvatus) with classical swine fever virus II: A comparative histopathological study.

    abstract::Wild African Suidae, the common warthog (Phacochoerus africanus) and bushpig (Potamochoerus larvatus), were experimentally infected with classical swine fever (CSF) virus following the diagnosis of CSF subtype 2.1 in domestic pigs in South Africa in 2005. No data regarding the susceptibility or potential lesions of th...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2010.01191.x

    authors: Gers S,Vosloo W,Drew T,Lubisi AB,Pardini A,Williams M

    更新日期:2011-04-01 00:00:00

  • Detection and genetic characterization of Porcine circovirus type 3 in Italy.

    abstract::Porcine circovirus type 3 (PCV3) was recently proposed as a new porcine circovirus. It has been described by researchers in the USA and China and associated with porcine dermatitis and nephropathy syndrome, reproductive failure and systemic inflammation disease. The study reports the occurrence of the new virus in Ita...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12714

    authors: Faccini S,Barbieri I,Gilioli A,Sala G,Gibelli LR,Moreno A,Sacchi C,Rosignoli C,Franzini G,Nigrelli A

    更新日期:2017-12-01 00:00:00

  • Avian metapneumovirus subtype C in Wild Waterfowl in Ontario, Canada.

    abstract::Avian metapneumovirus (aMPV) is an emerging poultry pathogen that has a significant economic impact on poultry production worldwide. The geographic range of the virus continues to expand, and wild birds have been implicated as reservoirs of aMPV that have the potential to spread the virus over long distances. Our obje...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12832

    authors: Jardine CM,Parmley EJ,Buchanan T,Nituch L,Ojkic D

    更新日期:2018-08-01 00:00:00

  • The role of land use patterns in limiting the spread of equine influenza in Queensland during the 2007 epidemic.

    abstract::In 2007, an epizootic of equine influenza (EI) occurred in Australia, involving parts of the states of Queensland and New South Wales. Following an extensive control program, the disease was eradicated within 4 months, after infecting more than 75,000 horses on over 10,000 properties. In Queensland, examination of lan...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2009.01080.x

    authors: East IJ

    更新日期:2009-10-01 00:00:00

  • Colostrum transfer of neutralizing antibodies against lumpy skin disease virus from vaccinated cows to their calves.

    abstract::The objective of the present study was to access the titres and duration of maternally derived neutralizing antibodies against lumpy skin disease virus (LSDV) in calves born to immunized dairy cows. The study was conducted in a Greek farm of 200 Holstein cows which were immunized with a homologous Neethling strain-bas...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12983

    authors: Agianniotaki EI,Babiuk S,Katsoulos PD,Chaintoutis SC,Praxitelous A,Quizon K,Boscos C,Polizopoulou ZS,Chondrokouki ED,Dovas CI

    更新日期:2018-12-01 00:00:00

  • Isolation and genomic characterization of gosling gout caused by a novel goose astrovirus.

    abstract::A severe infectious disease characterized with gout, haemorrhage and swellings of kidneys has affected goslings around the major goose-producing regions in China since November 2016. A Novel goose-origin astrovirus (AStV), designated as AStV/SDPY/Goose/1116/17 (AStV-SDPY) strain, was isolated from diseased goslings, a...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12928

    authors: Yang J,Tian J,Tang Y,Diao Y

    更新日期:2018-12-01 00:00:00

  • Genetic characterization and pathogenicity of a divergent broiler-origin orthoreovirus causing arthritis in China.

    abstract::Since 2013, there have been an increasing number of cases of arthritis in broilers caused by avian orthoreovirus (ARV) in China, and the virus remains highly virulent in chicks with high-level maternal antibodies. However, little information is available about the complete gene analysis and pathogenicity of the epidem...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13961

    authors: Jiang X,Lin Y,Yang J,Wang H,Li C,Teng X,Tang Y,Diao Y

    更新日期:2020-12-20 00:00:00

  • Serosurvey of Schmallenberg Virus Infections in Sheep and Goat Flocks in Lower Saxony, Germany.

    abstract::Schmallenberg virus (SBV) infections can cause congenital musculoskeletal and vertebral malformations as well as neurological failures in foetuses of several ruminant species if susceptible mother animals were infected during early gestation. Blood samples gained from 17 goat and 64 sheep flocks in Lower Saxony (LS), ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12161

    authors: Helmer C,Eibach R,Tegtmeyer PC,Humann-Ziehank E,Runge M,Ganter M

    更新日期:2015-08-01 00:00:00

  • Dynamic contact networks of swine movement in Manitoba, Canada: Characterization and implications for infectious disease spread.

    abstract::We use swine shipping data from Manitoba to construct one-mode dynamic contact networks of swine locations and two-mode location-to-truck networks at four time scales: daily, weekly, monthly and for the entire two-year study period. We provide measures of graph evolution and graph characterization for each, useful in ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13220

    authors: Augusta C,Taylor GW,Deardon R

    更新日期:2019-09-01 00:00:00

  • Genetic characterization of duck Tembusu virus in Thailand, 2015-2017: Identification of a novel cluster.

    abstract::Duck Tembusu virus (DTMUV) infected cases have increasingly been observed in several duck farms in Thailand since its first report in 2013. However, information on the genetic characteristic of DTMUVs recently circulating in ducks in Thailand is limited. In this study, we investigated the geographic distribution and g...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13230

    authors: Ninvilai P,Tunterak W,Oraveerakul K,Amonsin A,Thontiravong A

    更新日期:2019-09-01 00:00:00

  • Molecular and virological characterization of the first poultry outbreaks of Genotype VII.2 velogenic avian orthoavulavirus type 1 (NDV) in North-West Europe, BeNeLux, 2018.

    abstract::After two decades free of Newcastle disease, Belgium encountered a velogenic avian orthoavulavirus type 1 epizootic in 2018. In Belgium, 20 cases were diagnosed, of which 15 occurred in hobby flocks, 2 in professional poultry flocks and 3 in poultry retailers. The disease also disseminated from Belgium towards the Gra...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13863

    authors: Steensels M,Van Borm S,Mertens I,Houdart P,Rauw F,Roupie V,Snoeck CJ,Bourg M,Losch S,Beerens N,van den Berg T,Lambrecht B

    更新日期:2020-10-03 00:00:00

  • Network of contacts between cattle herds in a French area affected by bovine tuberculosis in 2010.

    abstract::France attained 'Officially Tuberculosis-Free' status in 2000. However, the Côte d'Or department (a French administrative unit) has since seen an increase in bovine tuberculosis (bTB) cases, with 35% of cases attributed to neighbourhood contamination. The aim of this study was to investigate the characteristics of nei...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2011.01269.x

    authors: Dommergues L,Rautureau S,Petit E,Dufour B

    更新日期:2012-08-01 00:00:00

  • First report of a novel goose astrovirus outbreak in Cherry Valley ducklings in China.

    abstract::A highly acute disease broke out in ducklings in Shandong Province in March 2019. The disease was characterized as visceral gout, with a mortality rate of 30%. The causative agent, which has given rise to similar symptoms in goslings, has been confirmed to be a novel goose astrovirus. The novel goose astrovirus, which...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13418

    authors: Chen H,Zhang B,Yan M,Diao Y,Tang Y

    更新日期:2020-03-01 00:00:00

  • Detection of piscine orthoreoviruses (PRV-1 and PRV-3) in Atlantic salmon and rainbow trout farmed in Germany.

    abstract::Piscine orthoreoviruses (PRVs) are emerging pathogens causing circulatory disorders in salmonids. PRV-1 is the etiological cause of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), characterized by epicarditis, inflammation and necrosis of the myocardium, myositis and necrosis of ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13018

    authors: Adamek M,Hellmann J,Flamm A,Teitge F,Vendramin N,Fey D,Riße K,Blakey F,Rimstad E,Steinhagen D

    更新日期:2019-01-01 00:00:00

  • Leishmaniasis in cat shelters: A serological, molecular and entomological study.

    abstract::An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the i...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13544

    authors: Leonel JAF,Vioti G,Alves ML,Benassi JC,Silva DTD,Spada JCP,Ruiz VLA,Starke-Buzetti WA,Soares RM,Oliveira TMFS

    更新日期:2020-03-12 00:00:00

  • Global SNP analysis of 11,183 SARS-CoV-2 strains reveals high genetic diversity.

    abstract::Since first identified in December of 2019, COVID-19 has been quickly spreading to the world in few months and COVID-19 cases are still undergoing rapid surge in most countries worldwide. The causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), adapts and evolves rapidly in nature. With the a...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13931

    authors: Yuan F,Wang L,Fang Y,Wang L

    更新日期:2020-11-18 00:00:00

  • First report of the novel atypical porcine pestivirus in Spain and a retrospective study.

    abstract::This study aimed to provide information regarding viral pathogenesis and molecular epidemiology linked with recently reported atypical porcine pestivirus (APPV) strains and to determine the circulation of APPV in Spain from 1997 to 2016. Two-day-old piglets with moderate-severe congenital tremor (CT) from a Spanish fa...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12699

    authors: Muñoz-González S,Canturri A,Pérez-Simó M,Bohórquez JA,Rosell R,Cabezón O,Segalés J,Domingo M,Ganges L

    更新日期:2017-12-01 00:00:00

  • Risk factors for highly pathogenic avian influenza in commercial layer chicken farms in bangladesh during 2011.

    abstract::A case-control study conducted during 2011 involved 90 randomly selected commercial layer farms infected with highly pathogenic avian influenza type A subtype H5N1 (HPAI) and 175 control farms randomly selected from within 5 km of infected farms. A questionnaire was designed to obtain information about potential risk ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12071

    authors: Osmani MG,Thornton RN,Dhand NK,Hoque MA,Milon SM,Kalam MA,Hossain M,Yamage M

    更新日期:2014-12-01 00:00:00

  • 'La fiebre de Malta': An Interface of Farmers and Caprine Brucellosis Control Policies in the Bajío Region, Mexico.

    abstract::This article shows that socio-economic factors, defined here as practices, knowledge, interests, beliefs and experiences have a role in the adoption of brucellosis control strategies in the Bajío region, Mexico. We combined qualitative and quantitative methods to show that socio-economic factors with regard to goat hu...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12359

    authors: Oseguera Montiel D,Udo HM,Frankena K,van der Zijpp A

    更新日期:2017-02-01 00:00:00

  • Avian Influenza H5N1 Surveillance and its Dynamics in Poultry in Live Bird Markets, Egypt.

    abstract::H5N1, a highly pathogenic avian influenza (H5N1 HPAI), is an endemic disease that is significant for public health in Egypt. Live bird markets (LBMs) are widespread in Egypt and play an important role in HPAI disease dynamics. The aim of the study was to evaluate the H5N1 HPAI prevalence in representative LBMs from 20...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12440

    authors: ElMasry I,Elshiekh H,Abdlenabi A,Saad A,Arafa A,Fasina FO,Lubroth J,Jobre YM

    更新日期:2017-06-01 00:00:00