Abstract:
:Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points of bacterial multiplication and the time-spans between the inoculum and the maximum of rickettsial copies were increased in consecutive order from eukaryotic cells, I. ricinus to D. marginatus systems. In dynamic system, multiplication maximum of R. slovaca was achieved 9 days earlier in I. ricinus; however, the number of rickettsial DNA copies was approximately 3.6 x 10(6) more in D. marginatus.
journal_name
Transbound Emerg Disjournal_title
Transboundary and emerging diseasesauthors
Spitalská E,Sparagano O,Boldis Vdoi
10.1111/j.1865-1682.2010.01104.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
70-1issue
1-2eissn
1865-1674issn
1865-1682pii
JVA1104journal_volume
57pub_type
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