Static and dynamic systems in Rickettsia slovaca life cycle evaluated by quantitative real-time polymerase chain reaction.

Abstract:

:Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points of bacterial multiplication and the time-spans between the inoculum and the maximum of rickettsial copies were increased in consecutive order from eukaryotic cells, I. ricinus to D. marginatus systems. In dynamic system, multiplication maximum of R. slovaca was achieved 9 days earlier in I. ricinus; however, the number of rickettsial DNA copies was approximately 3.6 x 10(6) more in D. marginatus.

journal_name

Transbound Emerg Dis

authors

Spitalská E,Sparagano O,Boldis V

doi

10.1111/j.1865-1682.2010.01104.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

70-1

issue

1-2

eissn

1865-1674

issn

1865-1682

pii

JVA1104

journal_volume

57

pub_type

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