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Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control.

Abstract：

:African swine fever (ASF) re-entered in Europe in 2007 by Georgia rapidly affecting neighbouring countries. Since then, ASF has caused severe problems to the Russian Federation (RF) and spread to Northern and Western regions, including Ukraine (2012 and 2014) and Belarus (2013). At the beginning of 2014, dead wild boars were found in Lithuania and Poland. Several outbreaks have been later notified in the European Union(EU), affecting domestic pigs and wild boar of Latvia, Lithuania and Poland, and also wild boar in Estonia, causing major problems for the EU pig sector. Some studies have been performed with this ASFV isolate, revealing that it belongs to genotype II and causes an acute form of the disease. However, few data are available about the presence of antibodies in field and experimental samples from the affected area. This study analysed samples from experimental infections with ASFV isolated from the RF in 2013 (74 sera and 3 tissue exudates), and field samples from the RF from 2013 to 2014 (266 samples, including 32 and 7 tissue exudates from domestic pigs and wild boar, respectively). All samples were tested by a commercial ELISA and, some of them (79), also by immunochromatographic tests. Positive and doubtful samples were confirmed by immunoblotting test. Positive results were found in experimental and field samples, which confirm the presence of antibodies against ASFV in the RF. Antibodies were detected in animals inoculated with three different ASFV isolates, with some differences found among them. Only a small percentage of field samples was positive for ASF antibodies (3.7%), in agreement with other observations that reported a high virulence for the ASFV isolates in the area. These results confirm the potential presence of survivor animals that should be considered in affected areas to help design effective control and eradication plans against ASF.

journal_name

Transbound Emerg Dis

journal_title

Transboundary and emerging diseases

authors

Mur L,Igolkin A,Varentsova A,Pershin A,Remyga S,Shevchenko I,Zhukov I,Sánchez-Vizcaíno JM

doi

10.1111/tbed.12304

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

e436-40

issue

eissn

1865-1674

issn

1865-1682

journal_volume

pub_type

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Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control.