Screening virulence factors of porcine extraintestinal pathogenic Escherichia coli (an emerging pathotype) required for optimal growth in swine blood.

Abstract:

:Porcine extraintestinal pathogenic Escherichia coli (ExPEC) is occurring with increasing frequency in China, which causes acute septicemia and sudden death in pigs leading to significant economic losses. Bacterial survival and even proliferation within host bloodstream are a common manifestation of a number of bacterial septicemias, including porcine ExPEC diseases. However, the underlying pathogenesis for this novel pathotype of ExPEC has not been explored deeply. Here, we used a conjunction with transposon mutagenesis to identify the mechanisms of bacterial fitness involved in optimal growth of porcine ExPEC in swine serum ex vivo under static culture. Our work identified 28 genes involved in nucleotide biosynthesis, extracellular polysaccharide biosynthesis, regulators Fur and FNR, acid/zinc resistance, and Deley-Douderoff carbon metabolism that are required for the serum fitness. Subsequent functional analyses revealed that either interruption of de novo nucleotide biosynthesis or blocking of several extracellular polysaccharide biosynthesis including O2-antigen, Lipid A-core, and ECA significantly affect porcine ExPEC's growth in swine serum and proliferation in host bloodstream. Furthermore, the reasonable regulations of iron and anaerobic metabolisms in response to host stimuli by global regulators Fur and FNR also play key roles during systemic infection of porcine ExPEC. These findings provide compelling evidences that de novo nucleotide biosynthesis may enable porcine ExPEC to adapt to swine blood-specific nutrient availability, and the effective assembly of O-antigen, lipid A-core, and ECA is required to resist the bactericidal activity of swine serum. These studies contribute to better understand the underlying mechanisms employed by porcine ExPEC to survive, grow in the swine bloodstream, and cause disease. These related factors may serve as therapeutic targets for countering or preventing ExPEC serum resistance in the clinic.

journal_name

Transbound Emerg Dis

authors

Ma J,Cheng Z,Bai Q,Zhao K,Pan Z,Yao H

doi

10.1111/tbed.13848

subject

Has Abstract

pub_date

2020-09-24 00:00:00

eissn

1865-1674

issn

1865-1682

pub_type

杂志文章
  • Emergence of Lineage IV Peste des Petits Ruminants Virus in Ethiopia: Complete Genome Sequence of an Ethiopian Isolate 2010.

    abstract::Isolates of peste des petits ruminants virus (PPRV) can be segregated genetically into four lineages. For decades, lineages I-III have been reported across Africa whilst lineage IV has predominantly circulated across Asia. However, the lineage distribution is currently changing in Africa. Importantly, full genome sequ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12287

    authors: Muniraju M,Mahapatra M,Ayelet G,Babu A,Olivier G,Munir M,Libeau G,Batten C,Banyard AC,Parida S

    更新日期:2016-08-01 00:00:00

  • HoBi-Like Pestivirus and Its Impact on Cattle Productivity.

    abstract::The clinical features and economic impact of the infection caused by an emerging group of pestiviruses, namely HoBi-like pestivirus, in a cattle herd of southern Italy are reported. In 2011, the virus was first associated with respiratory disease, causing an abortion storm after 1 year and apparently disappearing for ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12529

    authors: Decaro N,Lucente MS,Losurdo M,Larocca V,Elia G,Occhiogrosso L,Marino PA,Cirone F,Buonavoglia C

    更新日期:2016-10-01 00:00:00

  • Pathology and viral distributions of the porcinophilic foot-and-mouth disease virus strain (O/Taiwan/97) in experimentally infected pigs.

    abstract::Twenty-four specific pathogen free pigs were inoculated intradermally at the front-right heel bulb with 0.5 ml of viral suspension containing 10(6.0)tissue culture infectious dose (TCID(50)) with the porcinophillic strain (O/Taiwan/97) of foot-and-mouth disease virus (FMDV) isolated from the epizootic of FMD in Taiwan...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2009.01079.x

    authors: Lee SH,Jong MH,Huang TS,Lin YL,Wong ML,Liu CI,Chang TJ

    更新日期:2009-06-01 00:00:00

  • Coinfection of sheep with Anaplasma, Theileria and Babesia species in the Kurdistan Region, Iraq.

    abstract::Infections of small ruminants with Anaplasma, Theileria and Babesia species are widely distributed in the old world and are of great economic impact. In Iraq, data on disease occurrence in sheep caused by above-mentioned infectious agents are scarce. This study provides information on various haemoparasitic agents inf...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12148

    authors: Renneker S,Abdo J,Bakheit MA,Kullmann B,Beyer D,Ahmed J,Seitzer U

    更新日期:2013-11-01 00:00:00

  • Myxoma virus jumps species to the Iberian hare.

    abstract::The study of myxoma virus (MYXV) infections in the European rabbit (Oryctolagus cuniculus) has produced one of the most accepted host-pathogen evolutionary models. To date, myxomatosis has been limited to the European rabbit with sporadic reports in hares. However, reports of widespread mortalities in the Iberian hare...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13296

    authors: Dalton KP,Martín JM,Nicieza I,Podadera A,de Llano D,Casais R,Gimenez S,Badiola I,Agüero M,Duran M,Buitrago D,Romero LJ,García E,Parra F

    更新日期:2019-11-01 00:00:00

  • Genetic characterization and pathogenicity of a divergent broiler-origin orthoreovirus causing arthritis in China.

    abstract::Since 2013, there have been an increasing number of cases of arthritis in broilers caused by avian orthoreovirus (ARV) in China, and the virus remains highly virulent in chicks with high-level maternal antibodies. However, little information is available about the complete gene analysis and pathogenicity of the epidem...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13961

    authors: Jiang X,Lin Y,Yang J,Wang H,Li C,Teng X,Tang Y,Diao Y

    更新日期:2020-12-20 00:00:00

  • Molecular characterization of Peste des petits ruminants viruses in the Marmara Region of Turkey.

    abstract::Recent outbreaks of Peste des petits ruminants (PPR) in the Marmara region of Turkey including the European part of Thrace is important due to its proximity to Europe (Greece and Bulgaria) and the potential threat of spread of PPR into mainland Europe. In order to investigate the circulation of PPRV in the region susp...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13095

    authors: Altan E,Parida S,Mahapatra M,Turan N,Yilmaz H

    更新日期:2019-03-01 00:00:00

  • Transmission of influenza A virus in pigs.

    abstract::Influenza A virus infections cause respiratory disease in pigs and are a risk to public health. The pig plays an important role in influenza ecology because of its ability to support replication of influenza viruses from avian, swine and human species. Influenza A virus is widespread in pigs worldwide, and influenza A...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章,评审

    doi:10.1111/j.1865-1682.2011.01300.x

    authors: Torremorell M,Allerson M,Corzo C,Diaz A,Gramer M

    更新日期:2012-03-01 00:00:00

  • Gap analysis of Mycoplasma bovis disease, diagnosis and control: An aid to identify future development requirements.

    abstract::There is a worldwide problem of disease caused by Mycoplasma (M.) bovis in cattle; it has a significant detrimental economic and animal welfare impact on cattle rearing. Infection can manifest as a plethora of clinical signs including mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disor...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章,评审

    doi:10.1111/tbed.12860

    authors: Calcutt MJ,Lysnyansky I,Sachse K,Fox LK,Nicholas RAJ,Ayling RD

    更新日期:2018-05-01 00:00:00

  • Seroprevalence evolution of selected pathogens in Iberian wild boar.

    abstract::A total of 1279 Eurasian wild boar (Sus scrofa) sera were collected from 2000 to 2011 in the Iberian Peninsula to reveal time changes in serum antibody prevalences against selected infectious agents (porcine circovirus type 2, PCV2; porcine reproductive and respiratory syndrome virus, PRRSV; hepatitis E virus, HEV; an...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2011.01285.x

    authors: Boadella M,Ruiz-Fons JF,Vicente J,Martín M,Segalés J,Gortazar C

    更新日期:2012-10-01 00:00:00

  • Shedding of clade 2.3.4.4 H5N8 and H5N2 highly pathogenic avian influenza viruses in peridomestic wild birds in the U.S.

    abstract::European starlings (Sturnus vulgaris), house sparrows (Passer domesticus) and rock pigeons (Columba livia) are all wild birds commonly found in large numbers in and around human dwellings and domestic livestock operations. This study evaluated the susceptibility of these species to three strains of highly pathogenic a...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13147

    authors: Bosco-Lauth AM,Marlenee NL,Hartwig AE,Bowen RA,Root JJ

    更新日期:2019-05-01 00:00:00

  • Identification of emerging trends in the prevalence of dermatophytoses in alpacas (Vicugna pacos) farmed in Poland.

    abstract::The increasing number of dermatophytoses among animals observed recently in developed countries may be connected with relocation of many exotic species outside their natural living environment. Moreover, an impact on this situation may also be exerted by relapses related to limited compliance with antifungal treatment...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13624

    authors: Gnat S,Łagowski D,Nowakiewicz A,Osińska M,Dyląg M

    更新日期:2020-11-01 00:00:00

  • A potential role for ixodid (hard) tick vectors in the transmission of lumpy skin disease virus in cattle.

    abstract::Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Madagascar and the Middle East. The aim of this study was to investigate the potential role of ixodid (hard) ticks in the transmission of the disease. Cattle ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2010.01184.x

    authors: Tuppurainen ES,Stoltsz WH,Troskie M,Wallace DB,Oura CA,Mellor PS,Coetzer JA,Venter EH

    更新日期:2011-04-01 00:00:00

  • A novel NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) plays a limited role in the pathogenicity of porcine circoviruses (PCV2 and PCV3) and PRRSV co-infection.

    abstract::Co-infection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCVs) is commonly observed under field conditions and elicits more severe diseases than any singular infection. In this study, the co-infection of PRRSV, PCV2 and PCV3 was analyzed in tissue samples collected from 150...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13026

    authors: Chen N,Li S,Ye M,Huang Y,Huang Y,Xiao Y,Yu X,Dong J,Tian K,Zhu J

    更新日期:2019-01-01 00:00:00

  • Molecular detection of myxoma virus in the environment of vaccinated rabbitries.

    abstract::Myxoma virus (MYXV) is the aetiological agent of myxomatosis, a systemic, mostly lethal disease that affects European rabbits. Vaccination against it, although widespread, has not been completely effective and disease outbreaks still take place on farms which carry out vaccination programmes. Since some of these cases...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13809

    authors: López-Lorenzo G,López-Novo C,Prieto A,Díaz JM,Gullón J,Arnal JL,Benito A,Díaz P,Panadero R,Díez-Baños P,Dalton KP,Parra F,Fernández G

    更新日期:2020-08-19 00:00:00

  • Mycobacteria and disease in southern Africa.

    abstract::The genus Mycobacterium consists of over 120 known species, some of which (e.g. M. bovis and M. tuberculosis) contribute extensively to the burden of infectious disease in humans and animals, whilst others are commonly found in the environment but may rarely if ever be disease-causing. This paper reviews the mycobacte...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章,评审

    doi:10.1111/tbed.12159

    authors: Botha L,Gey van Pittius NC,van Helden PD

    更新日期:2013-11-01 00:00:00

  • Prevalence, distribution and antibiotic resistance of emergent Arcobacter spp. from clinically healthy cattle and goats.

    abstract::Prevalence, distribution and antibiotic resistance of Arcobacter spp. were investigated in cattle, goats, floor and treated water samples in this study. The prevalence of Arcobacter in adult and young was recorded as 8/110 (7.27%) and 4/83 (4.81%), respectively, which showed insignificant difference (P = 0.3503) in de...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2012.01311.x

    authors: Shah AH,Saleha AA,Zunita Z,Murugaiyah M,Aliyu AB,Jafri N

    更新日期:2013-02-01 00:00:00

  • Molecular detection of hepatitis E virus in wild boar population in eastern Romania.

    abstract::In industrialized countries, Hepatitis E is a recognized zoonosis, with wild boar and swine representing the main reservoirs for zoonotic genotype HEV-3 in Europe. Data related to HEV infection in wild boar population in Romania are restricted to serological surveys. Therefore, our main goal was to determine the HEV p...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12736

    authors: Porea D,Anita A,Demange A,Raileanu C,Oslobanu Ludu L,Anita D,Savuta G,Pavio N

    更新日期:2018-04-01 00:00:00

  • Detection of Bovine Viral Diarrhoea Virus 2 as the Cause of Abortion Outbreaks on Commercial Sheep Flocks.

    abstract::Outbreaks of abortions and lambs born with nervous clinical signs and/or congenital malformations affected different sheep farms in Spain. Initial diagnosis of 'border disease-like' was established, based on clinical signs, serology and/or RNA detection by a pan-pestivirus RT-PCR. However, further investigation using ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12599

    authors: Elvira Partida L,Fernández M,Gutiérrez J,Esnal A,Benavides J,Pérez V,de la Torre A,Álvarez M,Esperón F

    更新日期:2017-02-01 00:00:00

  • Identification and characterization of novel genotypes of psittacine beak and feather disease virus from budgerigar in China.

    abstract::Psittacine beak and feather disease (PBFD) is a common disease in psittacine bird that caused by beak and feather disease virus (BFDV). BFDV is widely spread and threatening psittacine birds worldwide. However, the BFDV infection in China remains largely unknown. In this study, a surveillance study of BFDV was conduct...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13274

    authors: Ma J,Tian Y,Zhang M,Wang W,Li Y,Tian F,Cheng Y,Yan Y,Sun J

    更新日期:2019-09-01 00:00:00

  • Analysis of surveillance systems in place in European Mediterranean countries for West Nile virus (WNV) and Rift Valley fever (RVF).

    abstract::West Nile virus (WNV) and Rift Valley fever virus (RVFV) represent an important group of viral agents responsible for vector-borne zoonotic diseases constituting an emerging sanitary threat for the Mediterranean Basin and the neighbouring countries. WNV infection is present in several Mediterranean countries, whereas ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章,多中心研究,评审

    doi:10.1111/tbed.12124

    authors: Cito F,Narcisi V,Danzetta ML,Iannetti S,Sabatino DD,Bruno R,Carvelli A,Atzeni M,Sauro F,Calistri P

    更新日期:2013-11-01 00:00:00

  • Rift Valley fever virus infections in Egyptian cattle and their prevention.

    abstract::Rift Valley fever virus (RVFV) causes consistently severe outbreaks with high public health impacts and economic losses in livestock in many African countries and has also been introduced to Saudi Arabia and Yemen. Egypt with its four large outbreaks in the last 40 years represents the northernmost endemic area of RVF...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12616

    authors: Mroz C,Gwida M,El-Ashker M,Ziegler U,Homeier-Bachmann T,Eiden M,Groschup MH

    更新日期:2017-12-01 00:00:00

  • Serosurvey of Schmallenberg Virus Infections in Sheep and Goat Flocks in Lower Saxony, Germany.

    abstract::Schmallenberg virus (SBV) infections can cause congenital musculoskeletal and vertebral malformations as well as neurological failures in foetuses of several ruminant species if susceptible mother animals were infected during early gestation. Blood samples gained from 17 goat and 64 sheep flocks in Lower Saxony (LS), ...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12161

    authors: Helmer C,Eibach R,Tegtmeyer PC,Humann-Ziehank E,Runge M,Ganter M

    更新日期:2015-08-01 00:00:00

  • Road-killed mammals provide insight into tick-borne bacterial pathogen communities within urban habitats.

    abstract::Small- and medium-sized mammals play an important role in the life cycle of tick-borne pathogens in urban habitats. Our aim was to apply the general protocol, DAMA (documentation-assessment-monitoring-action), which is an integrated proposal to build a proactive capacity to understand, anticipate, and respond to the o...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13019

    authors: Szekeres S,Docters van Leeuwen A,Tóth E,Majoros G,Sprong H,Földvári G

    更新日期:2019-01-01 00:00:00

  • Emergence of novel recombination lineage 3 of porcine reproductive and respiratory syndrome viruses in Southern China.

    abstract::Lineage 3 of porcine reproductive and respiratory syndrome viruses, which belong to North America type 2, has a long epidemic history in China. The novel lineage 3 viruses constantly emerging in recent years are characterized by a high detection rate and significant pathogenicity. In this study, we investigated the pr...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13067

    authors: Sun YK,Li Q,Yu ZQ,Han XL,Wei YF,Ji CH,Lu G,Ma CQ,Zhang GH,Wang H

    更新日期:2019-01-01 00:00:00

  • Highly Pathogenic Avian Influenza Virus A/H5N1 Infection in Vaccinated Meat Duck Flocks in the Mekong Delta of Vietnam.

    abstract::We investigated episodes of suspected highly pathogenic avian influenza (HPAI)-like illness among 12 meat duck flocks in two districts in Tien Giang province (Mekong Delta, Vietnam) in November 2013. In total, duck samples from 8 of 12 farms tested positive for HPAI virus subtype A/haemagglutinin 5 and neuraminidase 1...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12470

    authors: Cuong NV,Truc VN,Nhung NT,Thanh TT,Chieu TT,Hieu TQ,Men NT,Mai HH,Chi HT,Boni MF,van Doorn HR,Thwaites GE,Carrique-Mas JJ,Hoa NT

    更新日期:2016-04-01 00:00:00

  • First detection of foot-and-mouth disease virus O/ME-SA/ Ind2001e sublineage in Jordan.

    abstract::Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that is caused by the FMD virus (FMDV). This disease affects both wild and domestic cloven-hoofed animals, and the latter of which includes cattle, swine, sheep and goats. FMD is endemic to Jordan and has a severe impact on the productivity of domes...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13372

    authors: Ababneh MM,Hananeh W,Bani Ismail Z,Hawawsheh M,Al-Zghoul M,Knowles NJ,van Maanen K

    更新日期:2020-01-01 00:00:00

  • Analysis of Swine Movement in Four Canadian Regions: Network Structure and Implications for Disease Spread.

    abstract::Direct and indirect contacts among animal holdings are important in the spread of infectious diseases. The objectives of this study were to describe networks of pig movements and the sharing of trucks used for those movements between swine farms in four Canadian regions using network analysis tools and to obtain conta...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.12225

    authors: Thakur KK,Revie CW,Hurnik D,Poljak Z,Sanchez J

    更新日期:2016-02-01 00:00:00

  • Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily.

    abstract::Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the mic...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/j.1865-1682.2010.01131.x

    authors: Reale S,Lupo T,Migliazzo A,Di Mauro C,Ciprì V,Calderone S,Manna L,Vitale F

    更新日期:2010-04-01 00:00:00

  • Phylogeny, pathogenicity and transmissibility of a genotype XII Newcastle disease virus in chicken and goose.

    abstract::Although Newcastle disease virus (NDV) has a worldwide distribution, some NDV genotypes have more regional geographical ranges within continents. In this study, we isolated a subgenotype XIIb NDV strain, Goose/CH/GD/E115/2017 (E115), from geese in Guangdong province, Southern China, in 2017. Phylogenetic analysis show...

    journal_title:Transboundary and emerging diseases

    pub_type: 杂志文章

    doi:10.1111/tbed.13335

    authors: Xiang B,Chen R,Liang J,Chen L,Lin Q,Sun M,Kang Y,Ding C,Liao M,Xu C,Ren T

    更新日期:2020-01-01 00:00:00