Abstract:
:Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantum from dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantum isolates within each zymodema. Model- and distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.
journal_name
Transbound Emerg Disjournal_title
Transboundary and emerging diseasesauthors
Reale S,Lupo T,Migliazzo A,Di Mauro C,Ciprì V,Calderone S,Manna L,Vitale Fdoi
10.1111/j.1865-1682.2010.01131.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
37-41issue
1-2eissn
1865-1674issn
1865-1682pii
JVA1131journal_volume
57pub_type
杂志文章abstract::From 2014 to 2015, four novel highly pathogenic PRRS virus (HP-PRRSV) strains named 14LY01-FJ, 14LY02-FJ 15LY01-FJ, and 15LY02-FJ were isolated from high morbidity (100%) and mortality (40%-80%) in piglets and sows in Fujian Province. To further our knowledge about these novel virus strains, we characterized their com...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12617
更新日期:2017-12-01 00:00:00
abstract::Previous research has identified a relationship between climate and occurrence of SARS-CoV and MERS-CoV cases, information that can be used to reduce the risk of infection. Using COVID-19 notification and postcode data from New South Wales, Australia during the exponential phase of the epidemic in 2020, we used time s...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13631
更新日期:2020-11-01 00:00:00
abstract::Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Flavivirus genus and Flaviviridae family. The transmission of DTUMV involves mosquito vectors; however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. This study was conducted to examine DTMUV detecti...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
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更新日期:2020-05-01 00:00:00
abstract::The role and importance of pigs and pork as sources of zoonotic hepatitis E virus (HEV) has been debated in Canada and abroad for over 20 years. To further investigate this question, we compiled data to populate a risk profile for HEV in pigs or pork in Canada. We organized the risk profile (RP) using the headings pre...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.12582
更新日期:2017-12-01 00:00:00
abstract::Isolates of peste des petits ruminants virus (PPRV) can be segregated genetically into four lineages. For decades, lineages I-III have been reported across Africa whilst lineage IV has predominantly circulated across Asia. However, the lineage distribution is currently changing in Africa. Importantly, full genome sequ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12287
更新日期:2016-08-01 00:00:00
abstract::Mycoplasma hyopneumoniae (M. hyopneumoniae) is the aetiologic agent of enzootic pneumonia in swine, a prevalent chronic respiratory disease worldwide. Mycoplasma hyopneumoniae is a small, self-replicating microorganism that possesses several characteristics allowing for limited biosynthetic abilities, resulting in the...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
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更新日期:2019-09-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/j.1865-1682.2011.01300.x
更新日期:2012-03-01 00:00:00
abstract::Canine parvovirus type 2 (CPV-2) is an aetiological agent that causes acute haemorrhagic enteritis and fatal myocarditis in dogs. Since CPV-2 first emerged in the late 1970s, its rapid evolution has resulted in three antigenic variants: CPV-2a, CPV-2b and CPV-2c. Here, we report, for the first time in Korea, two cases...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13501
更新日期:2020-07-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13418
更新日期:2020-03-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,多中心研究,评审
doi:10.1111/tbed.12124
更新日期:2013-11-01 00:00:00
abstract::The aim of this study was to better understand the molecular epidemiology of Campylobacter coli isolated from multiple sources in Belgium, by studying the genotypic diversity and antimicrobial resistance phenotypes and resistance mechanisms of 59 C. coli isolates. Isolates from broiler carcasses and human cases were g...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13046
更新日期:2019-01-01 00:00:00
abstract::Recent European guidelines facilitate the use of emergency vaccines during outbreaks of foot-and-mouth disease. Antiviral drugs could be used as a complementary measure. This study aimed at developing a small animal model to assess the in vivo activity of early antiviral lead molecules with anti-foot-and-mouth disease...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2010.01169.x
更新日期:2010-12-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13156
更新日期:2019-05-01 00:00:00
abstract::Leishmaniosis is a tropical and subtropical vector-borne disease caused by hemoparasites of the genus Leishmania. The disease can infect humans, as well as domestic and wildlife animals. Dogs are the main reservoir for L. infantum, the aetiological agent of visceral leishmaniosis (VL) in America, and a domestic source...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13313
更新日期:2020-07-01 00:00:00
abstract::Porcine brucellosis is a disease caused by Brucella suis, which is characterized by reproductive disorders in pigs. The number of cases of swine brucellosis has risen in many European countries, likely because of the presence of a wild reservoir of B. suis in wild boar. This study aimed at evaluating factors that may ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12060
更新日期:2014-12-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13803
更新日期:2020-08-18 00:00:00
abstract::During 2012-2017, a total of 1,144 highly pathogenic avian influenza (HPAI) H5 outbreaks were reported in Taiwan. We conjectured the current 3-km radius of the post-outbreak containment policy could fail to effectively alleviate the current ongoing epidemics of HPAI H5 in Taiwan. The high intensity of localized transm...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13991
更新日期:2021-01-16 00:00:00
abstract::Newcastle disease (ND) is an economically important, contagious poultry viral disease reported across the globe. In India, ND is endemic and episodes of ND outbreaks despite strict vaccinations are not uncommon. We isolated and characterized seven ND viruses from vaccinated commercial poultry farms during severe disea...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13030
更新日期:2019-01-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12083
更新日期:2015-02-01 00:00:00
abstract::Canine parvovirus type 2 (CPV-2) emerged suddenly in the late 1970s as pathogen of dogs, causing a severe and often fatal gastroenteric disease. The original CPV-2 was replaced by three antigenic variants, CPV-2a, CPV-2b and CPV-2c, which to date have gained a worldwide distribution with different relative proportions...
journal_title:Transboundary and emerging diseases
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
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更新日期:2018-08-01 00:00:00
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journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13691
更新日期:2020-06-21 00:00:00
abstract::Wildlife and notably deer species--due to the increasing relevance of deer farming worldwide--may contribute to the maintenance of Coxiella burnetii, the causal agent of Q fever. Currently, there are no precedents linking exposure to deer species with human Q fever cases. However, a human case of Q fever was recently ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12179
更新日期:2015-10-01 00:00:00
abstract::Rickettsial infection in dog-associated ticks in three rural communities of Yucatan, Mexico was investigated using qPCR and nested PCR assays. A total of 319 dogs were studied and ticks samples were collected. A total of 170 dogs were infested with ticks (frequency of 53.4%). Overall, 1,380 ticks representing seven sp...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12990
更新日期:2019-01-01 00:00:00
abstract::The aim of our study was to determine species and genotypes of Cryptosporidium in patients suffering from immunosuppressive illnesses, but also in immunocompetent patients suffering from diarrhoea. A total of 80 samples of faeces were collected from both immunosuppressed and immunocompetent patients. The immunosuppres...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13009
更新日期:2019-01-01 00:00:00
abstract::Soon after the emergence of 2009 pandemic H1N1, the first outbreaks in breeder turkey operations were reported that implicated human-to-turkey transmission. In the spring of 2016, the reoccurrence of 2009 pandemic H1N1 lineage viruses infecting breeder turkey flocks in Ontario and Manitoba, Canada, also implicated hum...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12566
更新日期:2016-12-01 00:00:00
abstract::In the wake of on-going successful programmes for global eradication of rinderpest and the current effort to contain the spread of avian influenza, the progressive world-wide control of FMD must be regarded as a major contribution to the international public good. FMD is the single most animal disease constraint to in...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2007.01016.x
更新日期:2008-01-01 00:00:00
abstract::Outbreaks of trembling and shaking were reported among pigs at two pig farms in Jiangsu Province, China. Serum and tissue samples tested positive for porcine circovirus-like virus P1 and negative for classical swine fever virus, porcine circovirus type 2, astrovirus and porcine pestivirus using PCR/RT-PCR and immunohi...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12772
更新日期:2018-04-01 00:00:00
abstract::Highly pathogenic avian influenza (HPAI) risk management requires efficient surveillance of the infection in wild birds for early warning purposes. In this study, our aim was to describe the spread of continent-wide infection cases using a fireworks model and therefore improve current surveillance systems. The firewor...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13342
更新日期:2020-01-01 00:00:00
abstract::Direct and indirect contacts among animal holdings are important in the spread of infectious diseases. The objectives of this study were to describe networks of pig movements and the sharing of trucks used for those movements between swine farms in four Canadian regions using network analysis tools and to obtain conta...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12225
更新日期:2016-02-01 00:00:00