Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily.

Abstract:

:Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantum from dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantum isolates within each zymodema. Model- and distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.

journal_name

Transbound Emerg Dis

authors

Reale S,Lupo T,Migliazzo A,Di Mauro C,Ciprì V,Calderone S,Manna L,Vitale F

doi

10.1111/j.1865-1682.2010.01131.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

37-41

issue

1-2

eissn

1865-1674

issn

1865-1682

pii

JVA1131

journal_volume

57

pub_type

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