Isolation and Identification of Brucella melitensis Biovar 3 from Vaccinated Small Ruminants: A Public Health Threat in Kosovo.

Abstract:

:In 2011, a human brucellosis case with severe clinical symptoms was reported at the University Clinic for Infectious Diseases in Prishtina, Kosovo. A trace-back investigation was conducted to find the source of human infection. A total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised on the patient's farm were taken for serological and molecular analysis. Serology using RBT and CFT revealed 11 positive animals. Twelve milk samples were PCR positive. A Brucella strain isolated from a goat's milk sample was classified as Brucella melitensis biovar 3, indicating the first ever isolation and report in Kosovo. The use of the Bruce-ladder PCR provided differentiation between the field strain and the vaccine strain. Hence, the accidental transmission of the vaccine strain Rev 1 that was previously used for the vaccination of the farm animals could be excluded. The findings of this study show that brucellosis is still a public health threat in Kosovo despite control measures.

journal_name

Transbound Emerg Dis

authors

Hamidi A,Mayer-Scholl A,Dreshaj S,Robaj A,Sylejmani D,Ramadani N,Al Dahouk S,Nöckler K

doi

10.1111/tbed.12336

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

e296-e299

issue

6

eissn

1865-1674

issn

1865-1682

journal_volume

63

pub_type

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