Abstract：
AIMS:The mechanisms underlying the fetal origin of renal disease remains unknown. This study aimed to investigate the profiles of ion channel and transporter proteins in the fetal kidney in fetal growth restriction (FGR)rats, and to explore their association with the fetal origin of renal disease. MAIN METHODS:An FGR rat model was developed by administration of a low-protein diet. Then 367 differentially expressed proteins (DEPs) from quantitative proteome analysis were subjected to Ingenuity Pathway Analysis. 22 DEPs associated with ion channels/transporters were evaluated in the fetal kidney. Na+/H+ exchanger1(NHE1) and its downstream unfolded protein response (UPR) pathway were investigated. Furthermore, overexpression of NHE1 were achieved via plasmid transfection to evaluate the potential influence on the UPR pathway and cell apoptosis in human proximal tubular epithelial cell line HK2 cells. KEY FINDINGS:Findings were as follows: 1) In the FGR fetal kidney, aquaporin 2/4, solute carrier (SLC) 8a1, 33a1, etc. were downregulated, whereas other transporters including SLC 2a1, 4a1, 9a1, 29a3, etc. were upregulated. 2) NHE1 mRNA levels were markedly elevated in the FGR fetus. Further investigation revealed an increase in the UPR pathway regulators. 3) In vitro study showed that NHE1 overexpression in HK2 cells significantly induced expression of the endoplasmic reticulum stress (ERS) regulators and led to a decrease in the anti-apoptotic potential. SIGNIFICANCE:We speculate that maternal protein malnutrition causes dysregulation of ion channels/transporters in the fetal kidney. Upregulated NHE1 may activate the UPR pathway and induce cell apoptosis thus leading to impairment of kidney function.
journal_name
Life Scijournal_title
Life sciencesauthors
Guo Y,Lu Y,Wang J,Zhu L,Liu Xdoi
10.1016/j.lfs.2020.118276subject
Has Abstractpub_date
2020-10-15 00:00:00pages
118276eissn
0024-3205issn
1879-0631pii
S0024-3205(20)31028-6journal_volume
259pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Nitric oxide (NO) is a highly reactive free radical that is involved in a variety of different biological process. In recent reports, the putative role of NO in the neuropathogenesis of brain inflammation has been demonstrated. And then the relation between neuronal NO and convulsive seizures induced by virus has been...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.08.014
更新日期：2004-01-30 00:00:00
abstract：:Serotonin (5HT) stimulates phosphoinositide turnover in a number of tissues, but it is not known whether this effect is due to activation of a 5HT receptor which is coupled to phosphoinositide hydrolysis or if the effect is secondary to 5HT stimulated arachidonate metabolism or to the release of another neurotransmitt...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90060-3
更新日期：1986-02-17 00:00:00
abstract：:Neuropeptide Y (NPY) is a major hypothalamic peptide which is implicated in the regulation of energy balance and in the activation of the hypothalamo-pituitary adrenal axis. This study aimed primarily to determine the effects on regional hypothalamic NPY levels, of catabolism and weight loss induced in rats by the syn...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90020-p
更新日期：1992-01-01 00:00:00
abstract：:We have examined factors that alter the effect of diazepam (DZ) on plasma corticosterone (CS) in rats. DZ had a biphasic effect on plasma CS levels: CS decreased with doses below 5 mg/kg and increased with higher doses. Peak response occurred 90 minutes post injection in both sexes. Plasma DZ levels were significantly...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90367-0
更新日期：1988-01-01 00:00:00
abstract：AIMS:Despite the advanced cancer treatments, there is increased resistance to chemotherapy and subsequent mortality. In lack of reliable data in monolayer cultures and animal models, researchers are shifting to 3D cancer spheroids, which represents the in vivo robust tumour morphology. Calcium is essential in cell sign...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118291
更新日期：2020-11-01 00:00:00
abstract：:We investigated the effects of VA-045, an apovincaminic acid derivative, on isolated blood vessels. VA-045 (10(-7)-10(-5) M) and vinpocetine (10(-7)-10(-5) M) inhibited the 64 mM KCl-induced and 10(-6)M norepinephrine (NE)-induced contraction of rat aortic strips. VA-045 (10(-7)-10(-4) M) and vinpocetine (10(-7)-10(-4...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90115-j
更新日期：1993-01-01 00:00:00
abstract：:We developed an experimental model to study the effect of daily cocaine administration on the mucosal immune system during murine acquired immune deficiency syndrome (MAIDS). Mice were infected with LP-BM5 murine leukemia virus, a retrovirus which causes immunosuppression with development of functional murine AIDS. Mi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00874-4
更新日期：1994-01-01 00:00:00
abstract：:YM-09151-2(cis-N-(1-benzyl-2-methylpyrrolidin-3-yl) -5-chloro-2-methoxy-4-methylaminobenzamide) is a new benzamide which has been reported to be an antagonist of dopamine (DA) at D1-type DA receptors. In the present study, we have examined the extent of the interaction of YM-09151-2 with D2-type DA receptors in the an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90933-5
更新日期：1983-02-28 00:00:00
abstract：:Acute renal injury (AKI) is a risk factor for the development of hypertension, which involves oxidative stress, changes in Na+ handling, and the intrarenal renin-angiotensin-aldosterone system (RAAS) as underlying mechanisms. We investigated in rats whether renal ischemia-reperfusion (IR) leads to changes in the proxi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118879
更新日期：2021-02-01 00:00:00
abstract：AIMS:Data suggest pharmacological treatment of depression with selective serotonin reuptake inhibitors (SSRI) may impair bone health. Our group has previously modeled compromised craniofacial healing after treatment with sertraline, a commonly prescribed SSRI, and hypothesized potential culprits: alterations in bone ce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117827
更新日期：2020-08-15 00:00:00
abstract：AIMS:Excessive production of nitric oxide (NO) and reactive oxygen species (ROS) in sepsis modulates different cell functions. Since the sepsis severity is associated with the degree of platelet activation, we decided to investigate the role of systemic generation of NO and ROS in modulating the platelet adhesion of li...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.09.004
更新日期：2011-11-21 00:00:00
abstract：AIMS:3-Nitropropionic acid (3-NPA) is a naturally occurring fungal toxin that leads to ATP-depletion by inhibiting mitochondrial succinate dehydrogenase and produces chemical anoxia. The present study was conducted to identify the involvement of inhibitory system in 3-NPA-induced depression of spinal reflexes. METHODS...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.09.017
更新日期：2008-11-21 00:00:00
abstract：:The effects of three local anesthetics, lidocaine, dibucaine, and tetracaine, on Na+/H+ antiporter activity were examined in brush border membrane-reconstituted vesicles. Lidocaine at 10 microM inhibited H+ efflux in the presence of an inward Na+ gradient, suggesting that this anesthetic specifically inhibits the Na+/...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90172-n
更新日期：1990-01-01 00:00:00
abstract：:This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01276-0
更新日期：2001-09-07 00:00:00
abstract：AIMS:Septic shock, the severe form of sepsis, is associated with development of progressive damage in multiple organs. Kidney can be injured and its functions altered by activation of coagulation, vasoactive-peptide and inflammatory processes in sepsis. Endothelin (ET)-1, a potent vasoconstrictor, is implicated in the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.03.013
更新日期：2014-05-02 00:00:00
abstract：AIMS:Morus, a member of the family Moraceae and commonly known as the mulberry, comprises a pharmaceutically important plant group whose major constituents are the moracins. Moracin derivatives have received great attention because they exhibit a diverse range of biological functionalities. However, no studies have con...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.060
更新日期：2018-10-01 00:00:00
abstract：:The retina contains three catecholamine neurotransmitters: dopamine (DA); norepinephrine (NE); and epinephrine (EPI). DA and EPI appear to be associated with separate amacrine neurons that directly participate in the visual process. NE, in contrast, appears to be associated primarily with the sympathetic nerves that i...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(84)90184-x
更新日期：1984-09-10 00:00:00
abstract：AIMS:Dyslipidemia-associated diabetic retinopathy is featured by macular edema and retinal angiogenesis. This study investigated the in vitro lipotoxicity of free fatty acids and their modulatory roles in regulation of autophagy and angiogenic factor production in cultured human retinal pigment epithelium (RPE) ARPE-19...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118391
更新日期：2020-10-15 00:00:00
abstract：:Rats were made sensitive to the effects of the opioid antagonist naltrexone by treating them once weekly with cumulative doses of the drug (1, 3, 10, 30 and 100 mg/kg). Sensitization was monitored by measuring salivation following naltrexone administration. During the first week of treatment, no salivation was noted f...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90341-l
更新日期：1992-01-01 00:00:00
abstract：:Plasma 7B2-immunoreactivity (7B2-IR) concentrations in umbilical artery (UA), umbilical vein (UV) and maternal vein (MV) were measured by RIA at the time of obstetrical delivery at term. Plasma 7B2-IR concentrations (Mean +/- SEM) in UA (N = 12), UV (N = 16) and MV (N = 16) were 725 +/- 69, 699 +/- 64 and 116 +/- 4.5 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90744-2
更新日期：1987-10-19 00:00:00
abstract：:The enkephalin analogs, [D-Pen2,L-Cys5]- and [D-Pen2,D-Cys5]-enkephalin are cyclic compounds, conformationally constrained by virtue of their 14-membered, disulfide containing rings and by the rigidizing effect of the beta, beta dimethyl substituents of the penicillamine side chain. The analogs exhibit profound delta ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90239-4
更新日期：1983-05-30 00:00:00
abstract：:Morphine and the enkephalin-like peptide FK 33-824 given i.p. to rats potently inhibit gastrointestinal transit of a charcoal test meal, the doses inhibiting transit by 50% being respectively about 10 and 1 microgram/kg. These intestinal effects are opiate-specific, locally elicited and presumably involve the same act...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90359-9
更新日期：1982-09-20 00:00:00
abstract：:Rheumatoid arthritis is a chronic, inflammatory joint disease leading to inflammation of synovial membrane that lines the joints. This inflammation further progresses and results in destruction of joints and surrounding cartilages. The underlying factors can be oxidative stress, pro-inflammatory mediators, imbalance a...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118459
更新日期：2020-11-15 00:00:00
abstract：AIMS:Previous investigations by our group have shown that prenatal treatment with lipopolysaccharide (LPS; 100 μg/kg, intraperitoneally) on gestation day (GD) 9.5 in rats, which mimics infections by Gram-negative bacteria, induces short- and long-term behavioral and neuroimmune changes in the offspring. Because LPS ind...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.10.019
更新日期：2015-01-01 00:00:00
abstract：:alpha-Difluoromethylornithine (DFMO) directly infused into a brain-lateral ventricle (12.5, 25 and 50 micrograms/rat) dose- and time-dependently inhibited brain ODC activity. While having no influence per se on pain threshold, DFMO significantly inhibited the analgesic activity of morphine (15 mg/kg i.p.), this effect...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90614-0
更新日期：1989-01-01 00:00:00
abstract：:To establish a simple and sensitive quantitation of leukotriene B4 (LTB4), we developed a radioreceptor assay (RRA) using a highly specific [3H]leukotriene B4[( 3H]LTB4) binding to a guinea pig spleen homogenate. The assay detected LTB4 levels as low as 0.12 pmol per tube. Fifty percent inhibition of bound [3H]LTB4 wa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90376-1
更新日期：1988-01-01 00:00:00
abstract：:Tifluadom, although structurally a 1,4 benzodiazepine, has no affinity for the 3H-flunitrazepam binding site, but is a potent displacer of 3H-bremazocine from its opioid binding site. Tifluadom is characterised as an opiate kappa-receptor agonist in vitro and in vivo with potent analgesic activity in animals and no de...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90346-0
更新日期：1982-09-20 00:00:00
abstract：:Malic enzyme activity in the soluble fraction of the neonatal brain of hypothyroid rats was observed to be lowered as compared to that of the control animals (p less than 0.01). Administration of triiodothyronine to the neonates of control animals resulted in significant enhancement (p less than 0.001) in the activity...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90428-0
更新日期：1987-12-28 00:00:00
abstract：AIMS:Hypertension is one of the leading causes of cardiovascular mortality and morbidity. It is associated with severe cardiac and vascular dysfunction. Double-stranded RNA-dependent protein kinase (PKR), is a known inducer of inflammation and apoptosis. However, no research has been done to elucidate the role of the P...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118436
更新日期：2020-12-01 00:00:00
abstract：:The aim of the present study was to investigate the existence of aging- and sex-related alterations in the permeability of the blood-brain barrier (BBB) in the rat, by calculating a unidirectional blood-to-brain transfer constant (Ki) for the circulating tracer [14C]-alpha-aminoisobutyric acid. We observed that: a) th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90157-m
更新日期：1990-01-01 00:00:00
