Growth inhibition of cultured human glioma cells by beta-interferon is not dependent on changes in ganglioside composition.


:This investigation tested the hypothesis that the growth inhibiting effects of human beta-interferon on cultured human glioma cells involves changes in the ganglioside composition of these cells. Four cell lines derived from human malignant gliomas (12-18, U-251 MG, I29-A, 7-24) and two lines from human fetal brain (CHI, CHII) were cultured in the presence and in the absence of human beta-interferon (HuIFN-beta), 1,000 units per ml medium for three days before harvesting. Human beta-interferon had an inhibitory effect on growth of glioma but not fetal brain cells. Total ganglioside sialic acid for all cell lines ranged between 3.5 and 13.8 micrograms/10(7) cells (0.6-3.9 micrograms/mg protein). No distinct difference in the amount of total ganglioside per cell was observed between neoplastic and non-neoplastic cells, but the latter had more ganglioside per mg total protein. All cell lines displayed different patterns of gangliosides determined by high performance thin layer chromatography, but there was no distinct difference between glioma and fetal brain cells. Human beta-interferon increased the total amount of ganglioside per cell in one fetal brain and two glioma lines, but on a protein basis in only one glioma cell line (I29-A); HuIFN-beta had only minor effects on ganglioside patterns. There was a slight shift towards a greater proportion of structurally simpler gangliosides in both fetal brain and two glioma cell lines exposed to HuIFN-beta, but the reverse occurred in glioma U-251 MG. None of these changes strongly correlated with the degree of growth inhibition due to HuIFN-beta.(ABSTRACT TRUNCATED AT 250 WORDS)


Yates AJ,Markowitz DL,Stephens RE,Pearl DK,Whisler RL




Has Abstract


1988-03-01 00:00:00












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    abstract::The abundant axonal microtubule-associated protein tau regulates microtubule and actin dynamics, thereby contributing to normal neuronal function. We examined whether mice deficient in tau (Tau(-/-)) or with high levels of human tau differ from wild-type (WT) mice in their susceptibility to neuroaxonal injury in exper...

    journal_title:Journal of neuropathology and experimental neurology

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    authors: Weinger JG,Davies P,Acker CM,Brosnan CF,Tsiperson V,Bayewitz A,Shafit-Zagardo B

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    abstract::Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic transmission in autonomic ganglia, which innervate and control the activity of most visceral organs. By combining ultrastructural, immunocytochemical, and pharmacological analyses, we characterized the nAChR subtypes in the rat superior cervical ganglion...

    journal_title:Journal of neuropathology and experimental neurology

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    authors: Del Signore A,Gotti C,Rizzo A,Moretti M,Paggi P

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Murray JM

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    journal_title:Journal of neuropathology and experimental neurology

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Tennyson VM,Kremzner LT,Brzin M

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  • Identification of inter-species transmission of prion strains.

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Baron T

    更新日期:2002-05-01 00:00:00

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Smith ME,Forno LS,Hofmann WW

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    journal_title:Journal of neuropathology and experimental neurology

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Koch S,Molchanova SM,Wright AK,Edwards A,Cooper JD,Taira T,Gillingwater TH,Tyynelä J

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Jang B,Shin HY,Choi JK,Nguyen du PT,Jeong BH,Ishigami A,Maruyama N,Carp RI,Kim YS,Choi EK

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    journal_title:Journal of neuropathology and experimental neurology

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Garcia KL,Yu G,Nicolini C,Michalski B,Garzon DJ,Chiu VS,Tongiorgi E,Szatmari P,Fahnestock M

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    journal_title:Journal of neuropathology and experimental neurology

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    authors: Mikolaenko I,Pletnikova O,Kawas CH,O'Brien R,Resnick SM,Crain B,Troncoso JC

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    journal_title:Journal of neuropathology and experimental neurology

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Kuusisto E,Parkkinen L,Alafuzoff I

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  • Remyelination After Cuprizone-Induced Demyelination Is Accelerated in Juvenile Mice.

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Pfeifenbring S,Nessler S,Wegner C,Stadelmann C,Brück W

    更新日期:2015-08-01 00:00:00

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Ontell M,Paul HS,Adibi SA,Martin JL

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Nakamura M,Inoue HK,Ono N,Kunimine H,Tamada J

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Londoño D,Carvajal J,Arguelles-Grande C,Marques A,Cadavid D

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Nagy Z,Esiri MM,Jobst KA,Morris JH,King EM,McDonald B,Joachim C,Litchfield S,Barnetson L,Smith AD

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    journal_title:Journal of neuropathology and experimental neurology

    pub_type: 杂志文章


    authors: Hayashi M,Itoh M,Araki S,Kumada S,Shioda K,Tamagawa K,Mizutani T,Morimatsu Y,Minagawa M,Oda M

    更新日期:2001-04-01 00:00:00