miR-577 inhibits glioblastoma tumor growth via the Wnt signaling pathway.


:microRNAs (miRNAs) are commonly altered in glioblastoma. Publicly available algorithms suggest the Wnt pathway is a potential target of miR-577 and the Wnt pathway is commonly altered in glioblastoma. Glioblastoma has not been previously evaluated for miR-577 expression. Glioblastoma tumors and cell lines were evaluated for their expression of miR-577. Cell lines were transfected with miR-577, miR-577-mutant, or control mimics to evaluate the effect of miR-577 expression on cell proliferation in vitro and in an animal model. Wnt pathway markers were also evaluated for their association with miR-577 expression. miR-577 expression was decreased in 33 of 40 (82.5%) glioblastoma tumors and 5 of 6 glioblastoma cell lines. miR-577 expression correlated negatively with cell growth and cell viability. miR-577 down-regulation was associated with increased expression of the Wnt signaling pathway genes lipoprotein receptor-related protein (LRP) 6 (LRP6) and β-catenin. Western blot analysis confirmed decreased expression of the Wnt signaling pathway genes Axin2, c-myc, and cyclin D1 in miR-577 transfected cells. miR-577 expression is down-regulated in glioblastoma. miR-577 directly targets Wnt signaling pathway components LRP6 and β-catenin. miR-577 suppresses glioblastoma multiforme (GBM) growth by regulating the Wnt signaling pathway.


Mol Carcinog


Molecular carcinogenesis


Zhang W,Shen C,Li C,Yang G,Liu H,Chen X,Zhu D,Zou H,Zhen Y,Zhang D,Zhao S




Has Abstract


2016-05-01 00:00:00












  • Quantitation of 2-amino-3-methylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline DNA adducts in specific sequences using alkali or uvrABC excinuclease.

    abstract::2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MelQx) are carcinogens found in cooked meats that form DNA adducts upon metabolic activation. Purified DNA from Chinese hamster ovary (CHO) cells was reacted in vitro with the active metabolites N-acetoxy-IQ or N-acetoxy-Me...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Nouso K,Bohr VA,Schut HA,Snyderwine EG

    更新日期:1993-01-01 00:00:00

  • Long non-coding RNA-LET is a positive prognostic factor and exhibits tumor-suppressive activity in gallbladder cancer.

    abstract::The identification of cancer-associated long non-coding RNAs (lncRNAs) and the investigation of their molecular and biological functions are vital for understanding the molecular biology and progression of cancer. The lncRNA-LET, a newly identified lncRNA, was demonstrated to be down-regulated in hepatocellular cancer...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Ma MZ,Kong X,Weng MZ,Zhang MD,Qin YY,Gong W,Zhang WJ,Quan ZW

    更新日期:2015-11-01 00:00:00

  • The garlic compound ajoene targets protein folding in the endoplasmic reticulum of cancer cells.

    abstract::Ajoene is a natural allylsulfur compound found in crushed garlic that arrests growth and induces apoptosis in cancer cells. To gain mechanistic insights into the cytotoxicity of ajoene in cancer cells, two fluorescently labelled ajoene analogs with dansyl- (DP) and fluorescein- (FOX) tags were synthesized. The tagged ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kaschula CH,Hunter R,Cotton J,Tuveri R,Ngarande E,Dzobo K,Schäfer G,Siyo V,Lang D,Kusza DA,Davies B,Katz AA,Parker MI

    更新日期:2016-08-01 00:00:00

  • Effects of supplemental calcium and vitamin D on tight-junction proteins and mucin-12 expression in the normal rectal mucosa of colorectal adenoma patients.

    abstract::The physical gut barrier, comprised of a thick mucus layer and the epithelium, plays an important role in defense against microbes and foreign antigens. Calcium and vitamin D may be involved in maintaining the integrity of the intestinal mucosal barrier, the dysfunction of which may lead to endotoxemia and inflammatio...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Mandle HB,Jahan FA,Bostick RM,Baron JA,Barry EL,Yacoub R,Merrill J,Rutherford RE,Seabrook ME,Fedirko V

    更新日期:2019-07-01 00:00:00

  • Evidence that mutational activation of the ras genes may not be involved in aflatoxin B(1)-induced human hepatocarcinogenesis, based on sequence analysis of the ras and p53 genes.

    abstract::Exposure to aflatoxin B(1) (AFB(1)) is one of the risk factors for developing hepatoma. In rats, activation of the ras gene is a prevalent event in AFB(1)-induced hepatocarcinogenesis. It is not clear whether a similar event occurs in humans. By analysis of codon 249 of the p53 gene, six of 36 human hepatoma samples w...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chao HK,Tsai TF,Lin CS,Su TS

    更新日期:1999-10-01 00:00:00

  • Dishevelled-3 activates p65 to upregulate p120-catenin transcription via a p38-dependent pathway in non-small cell lung cancer.

    abstract::Dishevelled-3 (Dvl-3) and p120-catenin (p120ctn) have abnormal expression in non-small cell lung cancer (NSCLC), which is associated with poor prognosis. Dvl-3 upregulates p120ctn transcription in NSCLC cells, but the mechanism is unknown. Here we transiently transfected Dvl-3 cDNA to NSCLC cells. Dvl-3 transfection i...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhao H,Zhao Y,Jiang G,Zhang X,Zhang Y,Dong Q,Luan L,Papavassiliou P,Wang E,Wang E

    更新日期:2015-06-01 00:00:00

  • Expression level of miR-34a rather than P53 gene status correlates with mutability in related human lymphoblast cell lines.

    abstract::The P53 gene is a tumor suppressor gene and can prevent mutation and tumor induction though apoptosis and DNA repair when it is activated by genotoxic stress. miR-34a expression is regulated by the P53 gene and might be required for cell response to DNA damage. TK6 cells are human lymphoblast cells with normal P53 fun...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chen X,Yan J,Chen T

    更新日期:2012-08-01 00:00:00

  • Identification of differentially expressed genes in oral squamous cell carcinoma.

    abstract::Rapid advances in multimodality therapy have not significantly improved the overall 5-yr survival of oral cancer patients in the past two decades, thereby underscoring the need for molecular therapeutics. The development of new treatment strategies for more effective management of oral cancer requires identification o...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Arora S,Matta A,Shukla NK,Deo SV,Ralhan R

    更新日期:2005-02-01 00:00:00

  • Codon 61 mutations in the c-Harvey-ras gene in mouse skin tumors induced by 7,12-dimethylbenz[a]anthracene plus okadaic acid class tumor promoters.

    abstract::Three okadaic acid class tumor promoters, okadaic acid, dinophysistoxin-1, and calyculin A, have potent tumor-promoting activity in two-stage carcinogenesis experiments on mouse skin. DNA isolated from tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) and each of these tumor promoters revealed the same mutation ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Fujiki H,Suganuma M,Yoshizawa S,Kanazawa H,Sugimura T,Manam S,Kahn SM,Jiang W,Hoshina S,Weinstein IB

    更新日期:1989-01-01 00:00:00

  • A novel tumor suppressor gene RhoBTB2 (DBC2): frequent loss of expression in sporadic breast cancer.

    abstract::RhoBTB2 was isolated recently as a tumor suppressor gene from human chromosome 8p21.3. Although RhoBTB2 was found to be frequently lost in breast cancer lines, expression status of RhoBTB2 in sporadic breast cancer tissues and its clinical and prognostic value, however, remain unclear. Tissue samples from breast cance...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Mao H,Qu X,Yang Y,Zuo W,Bi Y,Zhou C,Yin H,Deng B,Sun J,Zhang L

    更新日期:2010-03-01 00:00:00

  • Arachidonoyl ethanolamide (AEA)-induced apoptosis is mediated by J-series prostaglandins and is enhanced by fatty acid amide hydrolase (FAAH) blockade.

    abstract::The endocannabinoid arachidonoyl ethanolamide (AEA) is a potent inducer of tumor cell apoptosis however its mechanism of cytotoxicity is unclear. A previous report from our laboratory showed that AEA induced cell death in a cyclooxygenase-2 (COX-2)-dependent manner and in this report our data indicate that AEA-induced...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kuc C,Jenkins A,Van Dross RT

    更新日期:2012-02-01 00:00:00

  • Gender specific association of TP53 polymorphisms (EX4 215G>C Arg72Pro, IVS3+40-41ins16, and IVS6+62G>A), with risk of oral cancer subtypes and overall survival of the patients.

    abstract::Reports on the association of TP53 polymorphisms with oral cancer are not only limited but also not specific to site and/or gender. Hence, we examined the effect of TP53 polymorphisms (EX4 215G>C, IVS3+40-41ins16 and IVS6+62G>A) on buccal mucosa cancer (BMC) and tongue cancer (TC) risk, survival of patients in relatio...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Nagam SL,Katta S,Prasad VV

    更新日期:2017-03-01 00:00:00

  • Putative tumor suppressor gene SEL1L was downregulated by aberrantly upregulated hsa-mir-155 in human pancreatic ductal adenocarcinoma.

    abstract::Sel-1-like (SEL1L) is a putative tumor suppressor gene that is significantly downregulated in human pancreatic ductal adenocarcinoma (PDA). The mechanism of the downregulation is unclear. Here, we investigated whether aberrantly upregulated microRNAs (miRNAs) repressed the expression of SEL1L. From reported miRNA micr...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Liu Q,Chen J,Wang J,Amos C,Killary AM,Sen S,Wei C,Frazier ML

    更新日期:2014-09-01 00:00:00

  • Malignant transformation of NIH 3T3 fibroblasts by human c-sis is dependent upon the level of oncogene expression.

    abstract::High-level expression of the c-sis oncogene, which encodes the beta chain of platelet-derived growth factor, transforms immortalized rodent fibroblasts in vitro to a malignant phenotype. c-sis gene expression has been demonstrated in a variety of human tumors, although generally at levels much lower than those shown t...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: MacArthur LH,Clarke MF,Westin EH

    更新日期:1992-01-01 00:00:00

  • Hypermethylation patterns in the Fhit regulatory region are tissue specific.

    abstract::DNA hypermethylation is associated with decreased expression of tumor suppressor genes. We previously observed decreased Fhit expression and Fhit promoter region hypermethylation in rodent tumors induced by various carcinogens, and noted that the 5' regulatory regions in the promoter, exon 1, and intron 1 were differe...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Guler G,Iliopoulos D,Han SY,Fong LY,Lubet RA,Grubbs CJ,Huebner K

    更新日期:2005-07-01 00:00:00

  • Induction of karyotype instability in a murine tumor cell line by quercetin, 2-amino-1-methyl-6 phenylimidazo[4,5-b]pyridine, and okadaic acid, as revealed by transmission distortion of the inactive X chromosome.

    abstract::Quercetin, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), and okadaic acid are found in various foods and have been shown to have mutagenic or promoter-like activity. The effects of these three compounds on the transmission of the inactive X chromosome were examined in MST-C6 murine tumor cells, which were de...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kuwabara K,Odani S,Takahashi Y,Arakawa M,Takagi N,Nagao M,Kominami R

    更新日期:1995-12-01 00:00:00

  • Inhibition of constitutively activated phosphoinositide 3-kinase/AKT pathway enhances antitumor activity of chemotherapeutic agents in breast cancer susceptibility gene 1-defective breast cancer cells.

    abstract::Loss or decrease of wild type BRCA1 function, by either mutation or reduced expression, has a role in hereditary and sporadic human breast and ovarian cancers. We report here that the PI3K/AKT pathway is constitutively active in BRCA1-defective human breast cancer cells. Levels of phospho-AKT are sustained even after ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Yi YW,Kang HJ,Kim HJ,Hwang JS,Wang A,Bae I

    更新日期:2013-09-01 00:00:00

  • Tumorigenic study on hepatocytes coexpressing SV40 with Ras.

    abstract::A model of neoplastic transformation by the combination of SV40 large T antigen (LT), SV40 small T antigen (ST), oncogenic Ras, and human telomerase reverse trasncriptase subunit (hTERT) has become established and replicated in primary human fibroblasts, however, there is no report on human hepatocytes. Here we use ce...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Sun B,Chen M,Hawks C,Hornsby PJ,Wang X

    更新日期:2006-04-01 00:00:00

  • Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor.

    abstract::The interaction of urokinase-type plasminogen activator (uPA) with its cell-surface receptor (uPAR) is implicated in diverse biological processes such as cell migration, tissue remodeling, and tumor cell invasion. Recent studies indicated that uPAR can act as an extracellular matrix receptor during cell adhesion. Rece...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chintala SK,Mohanam S,Go Y,Venkaiah B,Sawaya R,Gokaslan ZL,Rao JS

    更新日期:1997-12-01 00:00:00

  • Isosilybin A induces apoptosis in human prostate cancer cells via targeting Akt, NF-κB, and androgen receptor signaling.

    abstract::Prostate cancer (PCA) is the second most malignancy in American men. Advanced stage PCA cells possess unlimited replication potential as well as resistance to apoptosis. Therefore, targeting survival mechanisms and activating apoptotic machinery in PCA cells using nontoxic phytochemicals is suggested as an attractive ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Deep G,Gangar SC,Oberlies NH,Kroll DJ,Agarwal R

    更新日期:2010-10-01 00:00:00

  • Centchroman altered the expressions of tumor-related genes through active chromatin modifications in mammary cancer.

    abstract::Centchroman (CC), a female oral contraceptive, has been shown to possess breast anti-cancer activities. Recently, we have shown CC-mediated antimetastatic effect through reversal of epithelial-to-mesenchymal transition (EMT) in breast cancer. The loss of tumor suppressor genes (TSGs) has been shown to promote EMT in b...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Khan S,Shukla S,Sinha S,Meeran SM

    更新日期:2016-11-01 00:00:00

  • Antitumor Effect of Periplocin in TRAIL-Resistant gastric cancer cells via upregulation of death receptor through activating ERK1/2-EGR1 pathway.

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of the tumor necrosis factor family, induces apoptosis in a variety of cancer cells. However, gastric cancer (GC) cells are insensitive to TRAIL usually. In the previous study, we showed that Periplocin could induce apoptosis in GC cells via the...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhao LM,Li L,Huang Y,Han LJ,Li D,Huo BJ,Dai SL,Xu LY,Zhan Q,Shan BE

    更新日期:2019-06-01 00:00:00

  • Oncogene RNA helicase DDX6 promotes the process of c-Myc expression in gastric cancer cells.

    abstract::Human DEAD-box RNA helicase gene DDX6 was cloned from B-cell lymphoma cell line RC-K8. Previously, we reported that DDX6 acts as oncogene in several cancers such as colorectal cancer and hepatocellular carcinoma. However, the detailed mechanism of DDX6 action in carcinogenesis is largely unknown. In this study, we exa...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Taniguchi K,Iwatsuki A,Sugito N,Shinohara H,Kuranaga Y,Oshikawa Y,Tajirika T,Futamura M,Yoshida K,Uchiyama K,Akao Y

    更新日期:2018-05-01 00:00:00

  • Differential expression of the O6-alkylguanine-DNA alkyltransferase gene in normal human breast and skin tissue: in situ mapping of cell type-specific expression.

    abstract::O6-Alkylguanine is a major toxic, mutagenic, and carcinogenic lesion in cellular DNA that is repaired by O6-alkylguanine-DNA alkyltransferase (ATase). The expression of this gene is directly related to the cellular sensitivity of alkylating agents, with levels of this protein varying widely among human organs, tumors,...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Wani G,D'Ambrosio SM

    更新日期:1995-03-01 00:00:00

  • Assessment of mutations in Ki-ras and p53 in colon cancers from azoxymethane- and dimethylhydrazine-treated rats.

    abstract::Mutations in the Ki-ras oncogene and the p53 tumor suppressor gene are known to occur at high frequencies in human colon cancers. We measured the frequency of mutations in these two genes in colon adenocarcinomas obtained from a widely used experimental model of human colon carcinogenesis: F344 rats treated with the c...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Erdman SH,Wu HD,Hixson LJ,Ahnen DJ,Gerner EW

    更新日期:1997-06-01 00:00:00

  • A human endonuclease incises ultraviolet-irradiated DNA at cytosines and oxidized DNA at thymines.

    abstract::Both ultraviolet irradiation and oxidation of DNA produce a variety of pyrimidine base damages. A human endonuclease recognizes such altered bases on these DNA substrates. This human endonuclease incises ultraviolet-irradiated DNA exclusively at sites of photochemically modified cytosines. The precise sites of incisio...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Gallagher PE,Weiss RB,Brent TP,Duker NJ

    更新日期:1989-01-01 00:00:00

  • A p53 mutation in exon 5 associated with adenovirus transformation.

    abstract::The adenovirus type 5 (Ad5) 55-kDa E1B oncoprotein has been shown to form complexes with the p53 tumor suppressor protein. These complexes are thought to interfere with normal p53 activity and may be responsible for the paucity of p53 mutations in cells transformed by these viruses. This report describes an example of...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Eyler YL,Siwarski DF,Huppi KE,Lewis AM Jr

    更新日期:1995-01-01 00:00:00

  • The effect of the JE (MCP-1) gene, which encodes monocyte chemoattractant protein-1, on the growth of HeLa cells and derived somatic-cell hybrids in nude mice.

    abstract::To investigate the effect of tumor-associated macrophages on the in vivo growth properties of cervical carcinoma cells, tumorigenic human papilloma virus (HPV) 18-positive HeLa cells were transfected with an expression vector harboring the cDNA for the macrophage chemoattractant protein-1 JE (MCP-1). Although the endo...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kleine K,König G,Kreuzer J,Komitowski D,Zur Hausen H,Rösl F

    更新日期:1995-11-01 00:00:00

  • Molecular evidence supporting the precursor nature of atypical adenomatous hyperplasia of the prostate.

    abstract::Atypical adenomatous hyperplasia (AAH) of the prostate is characterized by lobular proliferation of closely packed small acini. It is hypothesized that AAH is a precursor lesion for low-grade prostate cancer arising from the transition zone. Telomere dysfunction is common during malignant transformation of epithelia. ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Cheng L,Montironi R,Davidson DD,Wang M,Lopez-Beltran A,Zhang S

    更新日期:2019-07-01 00:00:00

  • Osteoblasts-derived TGF-beta1 enhance motility and integrin upregulation through Akt, ERK, and NF-kappaB-dependent pathway in human breast cancer cells.

    abstract::Bone metastases are common complications of breast cancer. Integrins are the major adhesive molecules in mammalian cells. Here we found that osteoblast conditioned medium (OBCM) increased the migration and cell surface expression of beta1 or beta3 integrin in human breast cancer cells (MDA-MB-231 cells). beta1 or beta...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Wei YY,Chen YJ,Hsiao YC,Huang YC,Lai TH,Tang CH

    更新日期:2008-07-01 00:00:00