mSphere of Influence: No More Excuses-Addressing Race, Racism, and Socioeconomic Issues in the Science Classroom and Laboratory.

abstract：:Bacteria must sense alterations in their environment and respond with changes in function and/or structure in order to cope. Extracytoplasmic function sigma factors (ECF σs) modulate transcription in response to cellular and environmental signals. The symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium melilo...

journal_title：mSphere

authors： Lang C,Barnett MJ,Fisher RF,Smith LS,Diodati ME,Long SR

更新日期：2018-10-10 00:00:00

abstract：:A maternal vaccine capable of boosting neutralizing antibody (NAb) responses directed against circulating viruses in HIV-infected pregnant women could effectively decrease mother-to-child transmission of HIV. However, it is not known if an HIV envelope (Env) vaccine administered to infected pregnant women could enhanc...

journal_title：mSphere

authors： Hompe ED,Mangold JF,Kumar A,Eudailey JA,McGuire E,Haynes BF,Moody MA,Wright PF,Fouda GG,Giorgi EE,Gao F,Permar SR

更新日期：2020-06-03 00:00:00

abstract：:Outbreaks of infection occur more often than they are reported in most developing countries, largely due to poor diagnostic services. A Klebsiella species bacteremia outbreak in a newborn unit with high mortality was recently encountered at a location being surveilled for childhood bacteremia. These surveillance effor...

journal_title：mSphere

authors： Brinkac LM,White R,D'Souza R,Nguyen K,Obaro SK,Fouts DE

更新日期：2019-03-13 00:00:00

abstract：:This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing catheli...

journal_title：mSphere

authors： Sakoulas G,Kumaraswamy M,Kousha A,Nizet V

更新日期：2017-12-06 00:00:00

abstract：:The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates...

journal_title：mSphere

authors： Muñoz JF,Farrer RA,Desjardins CA,Gallo JE,Sykes S,Sakthikumar S,Misas E,Whiston EA,Bagagli E,Soares CM,Teixeira MM,Taylor JW,Clay OK,McEwen JG,Cuomo CA

更新日期：2016-09-28 00:00:00

abstract：:Bifidobacterium species are important commensals capable of dominating the infant gut microbiome, in part by producing acids that suppress growth of other taxa. Bifidobacterium species are less prone to possessing antimicrobial resistance (AMR) genes (ARGs) than other taxa that may colonize infants. Given that AMR is ...

journal_title：mSphere

authors： Taft DH,Liu J,Maldonado-Gomez MX,Akre S,Huda MN,Ahmad SM,Stephensen CB,Mills DA

更新日期：2018-09-26 00:00:00

abstract：:Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. Howeve...

journal_title：mSphere

authors： King JM,Kulhankova K,Stach CS,Vu BG,Salgado-Pabón W

更新日期：2016-06-08 00:00:00

abstract：:Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency. This pathogen has a tractable genetic system; however, gene disruption via electroporation remains difficult, while biolistic transformation is often limited by lack ...

journal_title：mSphere

authors： Wang P

更新日期：2018-06-13 00:00:00

abstract：:The rhoptry organelle is critical for the invasion of an erythrocyte by the malaria parasite Plasmodium falciparum. Despite their critical roles, the mechanisms behind their biogenesis are still poorly defined. Our earlier work had suggested that the interaction between the glycosylphosphatidylinositol (GPI)-anchored ...

journal_title：mSphere

authors： Hallée S,Boddey JA,Cowman AF,Richard D

更新日期：2018-01-03 00:00:00

abstract：:Antibiotic resistance is a global crisis that threatens our ability to treat bacterial infections, such as tuberculosis, caused by Mycobacterium tuberculosis Of the 10 million cases of tuberculosis in 2017, approximately 19% of new cases and 43% of previously treated cases were caused by strains of M. tuberculosis res...

journal_title：mSphere

authors： Harrison GA,Mayer Bridwell AE,Singh M,Jayaraman K,Weiss LA,Kinsella RL,Aneke JS,Flentie K,Schene ME,Gaggioli M,Solomon SD,Wildman SA,Meyers MJ,Stallings CL

更新日期：2019-09-11 00:00:00

abstract：:The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about ...

journal_title：mSphere

authors： Vila T,Montelongo-Jauregui D,Ahmed H,Puthran T,Sultan AS,Jabra-Rizk MA

更新日期：2020-08-05 00:00:00

abstract：:Antibiotic resistance has become a major public health concern as bacteria evolve to evade drugs, leading to recurring infections and a decrease in antibiotic efficacy. Systematic efforts have revealed mechanisms involved in resistance. Yet, in many cases, how these specific mechanisms accelerate or slow the evolution...

journal_title：mSphere

authors： Langevin AM,El Meouche I,Dunlop MJ

更新日期：2020-12-16 00:00:00

abstract：:Malaria is a devastating illness that causes approximately 500,000 deaths annually. The malaria-causing parasite (Plasmodium genus) uses the process of translational repression to regulate its growth, development, and transmission. As poly(A)-binding proteins (PABP) have been identified as critical components of RNA m...

journal_title：mSphere

authors： Minns AM,Hart KJ,Subramanian S,Hafenstein S,Lindner SE

更新日期：2018-01-10 00:00:00

abstract：:Staphylococcus aureus is a facultative intracellular pathogen that invades and replicates within many types of phagocytic and nonphagocytic cells. During intracellular infection, S. aureus is capable of subverting xenophagy and escaping to the cytosol of the host cell. Furthermore, drug-induced autophagy facilitates t...

journal_title：mSphere

authors： Bravo-Santano N,Ellis JK,Mateos LM,Calle Y,Keun HC,Behrends V,Letek M

更新日期：2018-08-08 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. In CRISPR-Cas9 genome editing methods designed for use in Candida albicans, DNAs that encode the necessary components are expressed in the target cells. ...

journal_title：mSphere

authors： Grahl N,Demers EG,Crocker AW,Hogan DA

更新日期：2017-06-21 00:00:00

abstract：:The emergence of Cryptococcus gattii, previously regarded as a predominantly tropical pathogen, in the temperate climate of the North American Pacific Northwest (PNW) in 1999 prompted several questions. The most prevalent among these was the timing of the introduction of this pathogen to this novel environment. Here, ...

journal_title：mSphere

authors： Roe CC,Bowers J,Oltean H,DeBess E,Dufresne PJ,McBurney S,Overy DP,Wanke B,Lysen C,Chiller T,Meyer W,Thompson GR 3rd,Lockhart SR,Hepp CM,Engelthaler DM

更新日期：2018-01-17 00:00:00

abstract：:Felipe Santiago-Tirado studies the cell biology of cryptococcal infections. In this mSphere of Influence article, he reflects on how the papers "Systematic Genetic Analysis of Virulence in the Human Fungal Pathogen Cryptococcus neoformans" (https://doi.org/10.1016/j.cell.2008.07.046) and "Unraveling the Biology of a F...

journal_title：mSphere

authors： Santiago-Tirado FH

更新日期：2019-10-02 00:00:00

abstract：:Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococcus neoformans on solid medi...

journal_title：mSphere

authors： Reis FCG,Borges BS,Jozefowicz LJ,Sena BAG,Garcia AWA,Medeiros LC,Martins ST,Honorato L,Schrank A,Vainstein MH,Kmetzsch L,Nimrichter L,Alves LR,Staats CC,Rodrigues ML

更新日期：2019-03-20 00:00:00

abstract：:The genomes of Leishmania and trypanosomes encode six paralogs of the eIF4E cap-binding protein, known in other eukaryotes to anchor the translation initiation complex. In line with the heteroxenous nature of these parasites, the different LeishIF4E paralogs vary in their biophysical features and their biological beha...

journal_title：mSphere

authors： Shrivastava R,Tupperwar N,Drory-Retwitzer M,Shapira M

更新日期：2019-09-04 00:00:00

abstract：:Members of family Coronaviridae cause a variety of diseases in birds and mammals. Porcine hemagglutinating encephalomyelitis virus (PHEV), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs ≤4 weeks of age. No commercial PHEV vaccines are available, and neonatal...

journal_title：mSphere

authors： Mora-Díaz JC,Magtoto R,Houston E,Baum D,Carrillo-Ávila JA,Temeeyasen G,Zimmerman J,Piñeyro P,Giménez-Lirola L

更新日期：2020-05-06 00:00:00

abstract：:To design immune interventions that can synergize with antiretroviral therapy (ART) to reduce the rate of HIV mother-to-child transmission (MTCT), it is essential to characterize maternal immune responses in the setting of ART during pregnancy and breastfeeding and define their effect on MTCT. Prior studies reported a...

journal_title：mSphere

authors： Hompe ED,Jacobson DL,Eudailey JA,Butler K,Edwards W,Pollara J,Brummel SS,Fouda GG,Chinula L,Kamanga M,Kinikar A,Moodley D,Owor M,Fowler MG,Permar SR

更新日期：2019-10-23 00:00:00

abstract：:In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation l...

journal_title：mSphere

authors： Stappler E,Dattenböck C,Tisch D,Schmoll M

更新日期：2017-05-10 00:00:00

abstract：:Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by the presence of a thick cell wall. One alternative to have better...

journal_title：mSphere

authors： Rizzo J,Chaze T,Miranda K,Roberson RW,Gorgette O,Nimrichter L,Matondo M,Latgé JP,Beauvais A,Rodrigues ML

更新日期：2020-08-12 00:00:00

abstract：:Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic, potentially accentuated by the organism's genome plasticity, in...

journal_title：mSphere

authors： Marton T,Maufrais C,d'Enfert C,Legrand M

更新日期：2020-09-02 00:00:00

abstract：:Clostridioides difficile is an obligately anaerobic, spore-forming, Gram-positive pathogenic bacterium that is considered the leading cause of nosocomial diarrhea worldwide. Recent studies have attempted to understand the biology of the outermost layer of C. difficile spores, the exosporium, which is believed to contr...

journal_title：mSphere

authors： Romero-Rodríguez A,Troncoso-Cotal S,Guerrero-Araya E,Paredes-Sabja D

更新日期：2020-11-18 00:00:00

abstract：:Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories usi...

journal_title：mSphere

authors： Balloch A,Roalfe L,Ekstrom N,Nguyen CD,Spry L,Marimla RA,Licciardi PV,Goldblatt D,Mulholland EK

更新日期：2018-04-25 00:00:00

abstract：:Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze th...

journal_title：mSphere

authors： Krishnananthasivam S,Sathkumara HD,Corea E,Natesan M,De Silva AD

更新日期：2017-04-19 00:00:00

abstract：:Experimental evolution is a method in which populations of organisms, often microbes, are founded by one or more ancestors of known genotype and then propagated under controlled conditions to study the evolutionary process. These evolving populations are influenced by all population genetic forces, including selection...

journal_title：mSphere

authors： Cooper VS

更新日期：2018-05-09 00:00:00

abstract：:We describe a novel genome integration system that enables the introduction of DNA fragments as large as 50 kbp into the chromosomes of recipient bacteria. This system, named BPI, comprises a bacterial artificial chromosome vector and phage-derived gene integration machinery. We introduced the wbm locus of Bordetella ...

journal_title：mSphere

authors： Ishigaki K,Shinzawa N,Nishikawa S,Suzuki K,Fukui-Miyazaki A,Horiguchi Y

更新日期：2018-01-24 00:00:00

abstract：:Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...

journal_title：mSphere

authors： Arzt J,Belsham GJ,Lohse L,Bøtner A,Stenfeldt C

更新日期：2018-09-12 00:00:00