Structural and physico-chemical effects of disease and non-disease nsSNPs on proteins.

abstract：:Covalently circularized nanodiscs (cNDs) represent a significant advance in the durability and applicability of nanodisc technology. The new cNDs demonstrate higher size homogeneity and improved stability compared with that of non-circularized forms. Moreover, cNDs can be prepared at various defined sizes up to 80-nm ...

journal_title：Current opinion in structural biology

authors： Nasr ML,Wagner G

更新日期：2018-08-01 00:00:00

abstract：:The encounter complex of two proteins is a dynamic intermediate state that guides proteins to their binding site, thus enhancing the rate of complex formation. It is particularly useful for complexes that must balance a biological requirement for high turnover with the need for specific binding, such as electron trans...

journal_title：Current opinion in structural biology

authors： Schilder J,Ubbink M

更新日期：2013-12-01 00:00:00

abstract：:Salt-dependent electrostatic effects are a major factor in determining the stability, structure, reactivity, and binding behavior of nucleic acids. Increasingly detailed theoretical methods, especially those based on Monte Carlo and Poisson-Boltzmann methodologies, combined with powerful computational algorithms are b...

journal_title：Current opinion in structural biology

authors： Sharp KA,Honig B

更新日期：1995-06-01 00:00:00

abstract：:The X-ray structures of two prokaryotic pentameric ligand-gated ion channels have recently provided detailed insight into this important family of neurotransmitter receptors. These prokaryotic homologs share the overall architecture of their eukaryotic counterparts with conservation in functionally important residues....

journal_title：Current opinion in structural biology

authors： Hilf RJ,Dutzler R

更新日期：2009-08-01 00:00:00

abstract：:Here we review mechanisms of protein evolution leading to structural changes in protein complexes. These mechanisms include mutations directly within protein interfaces, as well as the effects of mutations that propagate from distant regions of the protein. We also discuss the constraints protein complex structures im...

journal_title：Current opinion in structural biology

authors： Zhang X,Perica T,Teichmann SA

更新日期：2013-12-01 00:00:00

abstract：:An important goal of structural genomics is to complete the structural analysis of all the enzymes in metabolic pathways and to understand the structural similarities and differences. A preliminary glimpse of this type of analysis was achieved before structural genomics efforts with the glycolytic pathway and efforts ...

journal_title：Current opinion in structural biology

authors： Erlandsen H,Abola EE,Stevens RC

更新日期：2000-12-01 00:00:00

abstract：:Viruses with an icosahedrally symmetric protein capsid and a membrane infect hosts from all three domains of life. Similar architectural principles are shared by different viral families, as exemplified by double-stranded DNA viruses such as PRD1 and STIV. During virus assembly, the membrane lipids are selectively acq...

journal_title：Current opinion in structural biology

authors： Huiskonen JT,Butcher SJ

更新日期：2007-04-01 00:00:00

abstract：:Many proteins are composed of protein domains, functional units of common descent. Multidomain forms are common in all eukaryotes making up more than half of the proteome and the evolution of novel domain architecture has been accelerated in metazoans. It is also becoming increasingly clear that alternative splicing i...

journal_title：Current opinion in structural biology

authors： Light S,Elofsson A

更新日期：2013-06-01 00:00:00

abstract：:CCA-adding enzymes (tRNA nucleotidyltransferases) are responsible for the maturation or repair of the functional 3' end of tRNAs. These enzymes are remarkable because they polymerize the essential nucleotides CCA onto the 3' terminus of tRNA precursors without using a nucleic acid template. Recent crystal structures, ...

journal_title：Current opinion in structural biology

authors： Xiong Y,Steitz TA

更新日期：2006-02-01 00:00:00

abstract：:The winged helix proteins constitute a subfamily within the large ensemble of helix-turn-helix proteins. Since the discovery of the winged helix/fork head motif in 1993, a large number of topologically related proteins with diverse biological functions have been characterized by X-ray crystallography and solution NMR ...

journal_title：Current opinion in structural biology

authors： Gajiwala KS,Burley SK

更新日期：2000-02-01 00:00:00

abstract：:C-type lectins are the largest and most diverse family of mammalian carbohydrate-binding proteins. They share a common protein fold, which provides the unifying basis for calcium-mediated carbohydrate recognition. Their involvement in a multitude of biological functions is remarkable. Here, we review the variety of ta...

journal_title：Current opinion in structural biology

authors： Keller BG,Rademacher C

更新日期：2020-06-01 00:00:00

abstract：:Can a stereochemical recognition code explain sequence-specific protein-nucleic acid interactions? Whereas a code that is generally applicable to DNA-binding proteins of all known structural families is unattainable, the indications are that a code can describe at least some of the interactions of classical zinc finge...

journal_title：Current opinion in structural biology

authors： Choo Y,Klug A

更新日期：1997-02-01 00:00:00

abstract：:Investigating the in vitro refolding of proteins that naturally reside in biological membranes is a notoriously difficult task. Biophysical studies on model systems are beginning to provide a sound physical basis for membrane protein folding that should help to alleviate this problem. Highlights of these studies inclu...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curran AR

更新日期：1999-02-01 00:00:00

abstract：:Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is stabilized and aligne...

journal_title：Current opinion in structural biology

authors： Demeshkina N,Jenner L,Yusupova G,Yusupov M

更新日期：2010-06-01 00:00:00

abstract：:The first high-resolution structure of a P-type ATPase, that of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum, was published in 2000. This structure has provided many clues to how the Ca(2+)-ATPase might work, but no complete answers. The Ca(2+)-ATPase structure reveals no clear pathway from the cytoplas...

journal_title：Current opinion in structural biology

authors： Lee AG

更新日期：2002-08-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are increasingly realized to play diverse biological roles, ranging from molecular signaling to the formation of membraneless organelles. Their high degree of disorder makes them more challenging to study using the techniques of conventional structural biology, because any obse...

journal_title：Current opinion in structural biology

authors： Best RB

更新日期：2017-02-01 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:Solid-state NMR (ssNMR) offers insight into the formation of protein complexes and ligand binding for a large range of molecular sizes and binding affinities. Recent instrumental and methodological progress has enabled novel possibilities for using multi-dimensional ssNMR to study molecular 3D structures and interacti...

journal_title：Current opinion in structural biology

authors： Baldus M

更新日期：2006-10-01 00:00:00

abstract：:The exponential growth in the amount of genomic data published in recent years has led to increased efforts in analysing genomes for the presence of repeated sequences, which has in turn fostered the development of novel repeat recognition methods. This has resulted in a deepened understanding of the importance and ab...

journal_title：Current opinion in structural biology

authors： Heringa J

更新日期：1998-06-01 00:00:00

abstract：:Few biological macromolecular complexes exhibit the combination of massive size and hierarchical, symmetrical architecture embodied in icosahedral viruses. X-ray crystallography, electron cryomicroscopy and small-angle X-ray scattering provide complementary approaches to studying these remarkable structures. Through a...

journal_title：Current opinion in structural biology

authors： Lee KK,Johnson JE

更新日期：2003-10-01 00:00:00

abstract：:Cells have evolved intricate ways to propagate signals through signaling networks rich in crosstalks between signaling pathways and feedforward and feedback loops. The enzymatic products of phosphorylation-dependent signaling, namely phosphopeptides, can be identified and quantified systematically by mass spectrometry...

journal_title：Current opinion in structural biology

authors： Gingras AC,Wong CJ

更新日期：2016-12-01 00:00:00

abstract：:Protein design is still a challenging undertaking, often requiring multiple attempts or iterations for success. Typically, the source of failure is unclear, and scoring metrics appear similar between successful and failed cases. Nevertheless, the use of sequence statistics, modularity and symmetry from natural protein...

journal_title：Current opinion in structural biology

authors： Broom A,Trainor K,MacKenzie DW,Meiering EM

更新日期：2016-06-01 00:00:00

abstract：:Continuing investigations into protein-protein interactions have revealed their key role in regulating a wide range of cellular processes. Although efforts to modulate these interactions are more challenging and much less mature than work on conventional drug discovery pathways, significant progress has been made on s...

journal_title：Current opinion in structural biology

authors： Zhao L,Chmielewski J

更新日期：2005-02-01 00:00:00

abstract：:Demand for novel GPCR modulators is increasing as the association between the GPCR signaling pathway and numerous diseases such as cancers, psychological and metabolic disorders continues to be established. In silico structure-based drug design (SBDD) offers an outlet where researchers could exploit the accumulating s...

journal_title：Current opinion in structural biology

authors： Lee Y,Lazim R,Macalino SJY,Choi S

更新日期：2019-04-01 00:00:00

abstract：:Arf GTPases are major regulators of membrane traffic and organelle structure in eukaryotes where they recruit many different effectors, including components of vesicular coats, proteins that tether membranes, sort lipids or have diverse other functions in vesicular traffic, and bacterial proteins that divert Arf funct...

journal_title：Current opinion in structural biology

authors： Cherfils J

更新日期：2014-12-01 00:00:00

abstract：:Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction ...

journal_title：Current opinion in structural biology

authors： Nishino T,Ishino Y,Morikawa K

更新日期：2006-02-01 00:00:00

abstract：:Akt1-3 (Akt) are a subset of the AGC protein Ser/Thr kinase family and play important roles in cell growth, metabolic regulation, cancer, and other diseases. We describe some of the roles of Akt in cell signaling and the biochemical and structural mechanisms of the regulation of Akt catalysis by the phospholipid PIP3 ...

journal_title：Current opinion in structural biology

authors： Cole PA,Chu N,Salguero AL,Bae H

更新日期：2019-12-01 00:00:00

abstract：:Multivalent molecules with a precise array of recognition elements that interact with specific cell types are important for characterizing the topology of molecules on a cell surface. Applications ranging from the control of cellular signaling to drug delivery and tissue imaging rely on these surface-mapping molecules...

journal_title：Current opinion in structural biology

authors： Lee Y,Sampson NS

更新日期：2006-08-01 00:00:00

abstract：:TolC and its many homologues comprise an alpha-helical transperiplasmic tunnel embedded in the bacterial outer membrane by a contiguous beta-barrel channel, providing a large exit duct for diverse substrates. The 'channel-tunnel' is closed at its periplasmic entrance, but can be opened by an 'iris-like' mechanism when...

journal_title：Current opinion in structural biology

authors： Koronakis V,Andersen C,Hughes C

更新日期：2001-08-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are central to many physiological processes, including the uptake of nutrients, the non-classical secretion of signaling molecules and toxins, multidrug resistance and the development of human disease. As one might expect from this spectrum of translocation events, these ubiquit...

journal_title：Current opinion in structural biology

authors： Schmitt L,Tampé R

更新日期：2002-12-01 00:00:00