Abstract：
:This report describes a technique which permits a high yield of viable adult cardiac myocytes from the adult newt using enzymatic separation techniques at low temperature and high enzyme concentrations. Observations by light microscopy showed the isolated myocytes to have a distinctively slender morphology which consisted of a variable number of arm-like appendages radiating from the center of cells which were predominantly mononucleated. Atrial myocytes were typically observed to have two to three arm-like appendages while ventricular myocytes typically had three to six appendages. The majority of myocytes displayed normal fine structure when examined by transmission electron microscopy. Computerized image analysis revealed that atrial cells were significantly greater in cell length (192.9 +/- 53.4 microns) and in nuclear length (25 +/- 5.3 microns) and perimeter (59.2 +/- 10.7 microns) than were ventricular cells (162.8 +/- 39 microns, 23.6 +/- 5.1 microns and 57.4 +/- 11.1 microns, respectively), while cell widths and areas were greater in ventricular cells (16.5 +/- 4.7 microns and 1839.8 +/- 585.0 microns, respectively) than in atrial cells (13.2 +/- 3.1 microns and 1520.3 +/- 527.6 microns, respectively). Comparison of these data with previous descriptions of isolated amphibian and mammalian cardiac myocytes emphasizes species-related differences.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Tate JM,McDonnell TJ,Oberpriller JC,Oberpriller JOdoi
10.1016/0040-8166(87)90049-8subject
Has Abstractpub_date
1987-01-01 00:00:00pages
577-85issue
4eissn
0040-8166issn
1532-3072pii
0040-8166(87)90049-8journal_volume
19pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:When adult rat hepatocytes were cultured in plastic Petri dishes in a medium containing insulin and glucagon, supplementation with epidermal growth factor (EGF) had a pronounced effect on their viability, morphology, and biochemical integrity. Transmission and scanning electron microscopic studies showed that after 1 ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(84)90040-5
更新日期：1984-01-01 00:00:00
abstract：:In Drosophila subobscura the male produces two classes of motile spermatozoa that differ in total length and nucleus length. The significance of this within-ejaculate polymegaly is obscure. We have carried out an ultrastructural and cytochemical analysis of both sperm morphs to understand their possible role at fertil...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(96)80005-x
更新日期：1996-04-01 00:00:00
abstract：:Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...
journal_title：Tissue & cell
pub_type： 临床试验,杂志文章
doi：10.1054/tice.1999.0005
更新日期：1999-02-01 00:00:00
abstract：:The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(85)90022-9
更新日期：1985-01-01 00:00:00
abstract：:As determined by light microscopic autoradiography, parts of the nervous system of Phocanema decipiens have selective and high affinity mechanisms for the uptake of tritiated noradrenaline, dihydroxyphenylalanine (dopa) and 5-hydroxytryptamine. In the nervous system, noradrenaline is accumulated only by the four papil...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(76)90003-3
更新日期：1976-01-01 00:00:00
abstract：:The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90023-3
更新日期：1990-01-01 00:00:00
abstract：:The incorporation of H(3)-histidine and H(3)-glucosamine by ovarian follicles of cecropia moths during incubation in female blood was followed autoradicgraphically. Labeling was most prominent in the follicle cells, the spaces between these cells, and the nascent yolk spheres in the oocyte cortex. Results of pulse-cha...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(69)80037-6
更新日期：1969-01-01 00:00:00
abstract：:The epidermal eyespots of the acoel Otocelis rubropunctata would appear to consist of a single cell type provided with pigment granules and cilia. Very numerous dictyosomes characterize the cytoplasm of the cells. The cilia possess the 9X2 + 2 pattern of microtubules and the typical rootlets of acoels. Microvilli are ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90082-k
更新日期：1990-01-01 00:00:00
abstract：:This paper describes the fine structure of the cockroach subgenual organ, a complex ciliated mechanoreceptor that detects vibrations in the substrate upon which the animal stands. Located beneath the knee in each walking leg, the cockroach subgenual organ is a thin, fan-shaped flap of tissue slung across the dorsal bl...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(75)80009-7
更新日期：1975-01-01 00:00:00
abstract：:Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/j.tice.2017.03.002
更新日期：2017-04-01 00:00:00
abstract：:We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.08.005
更新日期：2014-12-01 00:00:00
abstract：:A morphological and ultrastructural study was carried out on the spermatophore and spermatodoses of some species of Orthoptera Tettigoniidae. From the results concerning the spermatophore it emerged that this structure has a morphological and ultrastructural organization represented by a dilated ampulla and a peduncle...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040816602000344
更新日期：2002-10-01 00:00:00
abstract：:Microstructured surfaces mimicking the endothelial cell (EC) morphology is a new approach to improve the blood compatibility of synthetic vascular grafts. The ECs are capable of changing their shapes depending on different shear conditions. However, the quantitative correlation between EC morphology and shear stress h...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.12.005
更新日期：2015-04-01 00:00:00
abstract：:Three basic sublayers are identified in the epicuticle of the mineralised sclerites of the Atlantic shore crab Carcinus maenas (Crustacea, Decapoda): the surface coat, the cuticulin layer, and the inner epicuticle. Their morphogenesis and subsequent changes are described throughout the moulting cycle in the normal cut...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(05)80061-8
更新日期：1995-10-01 00:00:00
abstract：:Degeneration of intervertebral disk (IVD) has been increased in recent years. The lumbar herniation can be cured using conservative and surgical procedures. Surgery is considered after failure of conservative treatment. Partial discectomy, fusion, and total disk replacement (TDR) are also common surgical treatments fo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.05.008
更新日期：2014-10-01 00:00:00
abstract：:The gills of Penaeus aztecus were examined by light and electron microscopy. They are dendrobranchiate, consisting of a central axis with biserially arranged branches that subdivide into bifurcating filaments. A septum divides the lumina of these structures into afferent and efferent channels. Hemolymph from the stern...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(78)90008-3
更新日期：1978-01-01 00:00:00
abstract：:The oocyte cortex undergoes dramatic changes during oogenesis in Rhodnius prolixus. Despite numerous studies examining oogenesis in the telotrophic ovariole, none has investigated the ultrastructural details of the oocyte cortex, in particular, the lateral cortical cytoskeleton. Indirect immunofluorescent staining of ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(93)90081-u
更新日期：1993-06-01 00:00:00
abstract：:Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(71)80029-0
更新日期：1971-01-01 00:00:00
abstract：:In this study, the olfactory system of a semi-aquatic turtle, the snapping turtle, has been morphologically investigated by electron microscopy, immunohistochemistry, and lectin histochemistry. The nasal cavity of snapping turtle was divided into the upper and lower chambers, lined by the sensory epithelium containing...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.03.011
更新日期：2016-06-01 00:00:00
abstract：:Megalocytivirus infection is a major threat in rock bream aquaculture in Korea. To produce a highly concentrated megalocytivirus, primary cells, established cell line and persistently infected cell line were used in this study. Megalocytivirus was inoculated in primary fin cell cultures of red sea bream (Pagrus major)...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101387
更新日期：2020-10-01 00:00:00
abstract：:The sensory organs on the tarsi of the antenniform first legs of the whip spider Admetus pumilio C. L. Koch (Amblypygi, Arachnida) were examined with the scanning and transmission electron microscope. At least four different types of hair sensilla were found: (1) thick-walled bristles, which have the characteristics o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(75)90009-9
更新日期：1975-01-01 00:00:00
abstract：:In the present investigation, we have studied peroxisomes and sterol carrier protein-2 (SCP2) in control and luteinizing hormone stimulated rat luteal cells. Superovulated immature rats in mid-luteal phase (8 days after ovulation) were divided into two groups (n = 4/group) and treated with vehicle (0.2 ml saline), or ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(05)80056-4
更新日期：1995-10-01 00:00:00
abstract：:1. To be mechanically effective, supporting structures which are helicoidal need to be monodomain, with planar or concentric layers. 2. To achieve this in cholesteric liquid crystalline chemical models, a constraining surface is required. 3. The prediction which logically follows from this is that natural helicoidal s...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(88)90013-4
更新日期：1988-01-01 00:00:00
abstract：:The contribution of radiosensitive cells and macrophages to myocardial immunopathology has been studied in rats inoculated with Trypanosoma cruzi, Y strain. Immunodepression was induced by gamma irradiation and depletion of radioresistant macrophages was achieved by silica, a selective cytotoxic agent for macrophages....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.1999.0040
更新日期：1999-06-01 00:00:00
abstract：:Collodial gold coated with the glycoprotein, bovine submaxillary mucin (BSM-gold), was used to localize chemoreceptors known to be involved in triggering the discharge of cnidae in sea anemones. BSM-gold binds exclusively at the apical surface of the supporting cell, the cell adjacent to the cnidocyte (Watson and Hess...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90016-4
更新日期：1987-01-01 00:00:00
abstract：:The basal plate of the scales of Amia calva is composed of regular double twisted plywood, as in Latimeria and Dipnoan scales. However, the progressive rotation of the fibrils direction is left-handed in Amia and right-handed in the 'Sarcopterygians'. So, the similarity between these peculiar plywoods is probably the ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(81)90046-x
更新日期：1981-01-01 00:00:00
abstract：:Junctional complexes between the epithelial cells in the four distinct regions of the glow-worm Malpighian tubule were investigated by electron microscopy using thin sectioning, freeze-fracturing, osmotic disruption and tracer techniques. The lateral plasma membranes of all four cell types are joined by smooth septate...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(80)90011-7
更新日期：1980-01-01 00:00:00
abstract：:Granulocytes (GRs) and/or plasmatocytes (PLs), the two major immunocytes in arthropods, participate in cellular encapsulation of foreign tissue. Although gap and desmosome junctions have been reported in insect capsules, smooth septate junctions are being reported for the first time by both thin section and freeze-fra...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(88)90064-x
更新日期：1988-01-01 00:00:00
abstract：:The expression of adhesion molecules on the vascular endothelium of healthy and inflamed human dental pulp was studied immunohistochemically using antibodies for selectin family and immunoglobulin super-family members. In healthy dental pulp, some vessels reacted very weakly with major histocompatibility complex (MHC)...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80077-3
更新日期：1998-04-01 00:00:00
abstract：:Locust-style and pseudo-orthogonal structures were found in the cuticle of Heteropteryx dilatata, with the tibiae and femora containing both, and the ocular facets showing only helicoidal construction. The same epidermal cells appear to be competent to secrete both locust-style and pseudo-orthogonal cuticle structure....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90103-e
更新日期：1992-01-01 00:00:00
