Abstract:
OBJECTIVE:To study the mechanism of early chemosensitivity and later chemoresistance of Cytarabine (Cyt) to HL-60 cells to regulate ERK/Slug signal pathway. MATERIALS AND METHODS:HL-60 cells were stably transfected with NF-κBp65 siRNA,P53 up-regulated modulator of apoptosis (PUMA) siRNA (PUMA siRNA) and Slug siRNA respectively or exposed to 25 nM U0126 for 24h, then the cells were exposed to Cyt (250 nM) respectively. In different time point after Cyt treatment, the activity of NF-κB and the expression of Erk1/2, NF-κBp65, Slug and PUMA protein were detected. The cells survival rate and apoptotic index were detected by MTT and TUNEL methods. RESULTS:After the HL-60 cells were exposed to Cyt (250 nM) for 24 h, the activity of NF-κB reached maximum value, and reached minimum value for 48 h. The expressions of NF-κBp65 and PUMA reached maximum value after exporser to Cyt (250 nM) for 24 h, and reached minimum value after 48 h. Erk1/2 phosphorylation and Slug expression began to increase after exporser to Cyt (250 nM) for 24 h, and reached maximum value for 48 h. The cells survival rate was decreased, and arrived to minimum value (29.5 ± 4.6, F = 12.74, p < 0.01). The cells apotosis rate was increased, and arrived to maximum value (14.2 ± 2.8, F = 15.02, p < 0.01). By inhibiting NF-κBp65 or PUMA by siRNA transfection, the cells survival rate was enhanced and the apotosis rate was significantly declined after exporsed to Cyt (250 nM). When NF-κBp65 was inhibited, the expression of PUMA was decreased. By inhibiting Erk1/2 phosphorylation and Slug expression, the cells survival rate began to decrease for 24 h, and reached the fewest at 48 h after exporsed to Cyt (250 nM) (19.8 ± 2.7, F = 11.4, p < 0.01 and 17.4 ± 0.6, F = 15.3, p < 0.01). The cells apotosis rate began to increase from 24 h, and reach a head at 48 h after exporser to Cyt (250 nM) (28.6 ± 4.7, F = 9.84, p < 0.01 and 27.6 ± 6.4, F = 10.31, p < 0.01. After Erk1/2 phosphorylation inhibited, Slug expression was lowered and PUMA expression was significantly increased. The changes are most distinct after the cells were exporser to Cyt (250 nM) for 48 h. By Slug inhibition had no effect on Erk1/2 phosphorylation, but increase the expression of PUMA. CONCLUSIONS:The chemosensitiveness of HL-60 cells to Cyt were relation to the up-regulation of PUMA induced by NF-κB. The chemoresistance to Cyt was related to the up-regulation of Slug induced by Erk1/2, which inhibited the expression of PUMA.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Liu GJ,Pan GJ,Wang J,Wang LN,Xu Y,Tang Ysubject
Has Abstractpub_date
2014-01-01 00:00:00pages
3802-9issue
24eissn
1128-3602issn
2284-0729journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Most ulcers occur with slough and need debridement treatment. Surgical treatment is usually performed but many patients need an additional chemical therapy to promote healing process. This type of wound bed preparation is slower than surgical one, but it is essential in those patients who are not eligible fo...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Methanol leaf extracts of two Ethiopian traditional medicinal plants viz., Lomisar [vernacular name (local native language, Amharic); Cymbopogon citratus (DC) Stapf. (Poaceae)] and Bisana [vernacular name (local native language, Amharic); Croton macrostachyus Del. (Euphorbiaceae)] were screened for larvicidal activity...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:Nasopharyngeal carcinoma (NPC) is a common cancer with high incidence in Southern China. Taxol is one of the first-line chemotherapeutic drugs for treating NPC; however, Taxol resistance has become the main difficulty for clinical treatment and the mechanisms remain not fully understood. In this study, we mai...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202101_24375
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVE:Determination of the active fraction and compounds of the dichloromethanol extract of Schinus molle seeds and evaluation of their biological effects. MATERIALS AND METHODS:Dried seeds of Schinus molle were sequentially extracted in hexane, acetyl acetate and dichloromethane. The dichloromethane extract was s...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:Even when thyroidectomy preserves vocal cord motility it may leave patients with changes in voice quality. Although superior laryngeal nerve (LSN) damage after thyroidectomy manifests with aspecific symptoms, laryngoscopy discloses only slight morphological changes that are difficult to assess. We want to in...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Non-Muscle-Invasive-Bladder-Cancer represents 75-85% of the new bladder cancer cases per year. Trans-uretral vesical resection is the milestone for diagnosis and therapy. After primary treatment, recurrence is frequent depending on the presence of several established risk factors: multiplicity, T dimension, prior recu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:The X-linked inhibitor of apoptosis protein (XIAP) is associated with the development of various tumors. The abnormal miR-142 expression is associated with the onset of lung cancer. Bioinformatics analysis revealed a targeted relationship between miR-142 and XIAP. This report investigated whether miR-142 play...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201909_18852
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:Considering the knowledge gap between underlying pathophysiological mechanisms of oxidative stress and increased cardiovascular risk, the present study aimed to examine the potential relationship between total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) and the Reynol...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202010_23232
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To uncover the role of UPF1 in alleviating the progression of glioma via targeting long non-coding RNA (lncRNA) CYTOR and underlying mechanism. PATIENTS AND METHODS:A total of 30 glioma tissues surgically resected from glioma patients and 30 brain tissues were collected from brain trauma patients undergoing ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19567
更新日期:2019-11-01 00:00:00
abstract::The pandemic influenza A H1N1 will affect millions of subjects. This influenza can cause respiratory complications with possible death. We have described two case reports of acute severe asthma exacerbation combined to influenza A H1N1, caracterized by severe respiratory failure. The diagnosis of influenza A H1N1 was ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract::This short review describes the conceptual development in the search for the enteric neural circuits with the initial identifications of the classes of enteric neurons on the bases of their morphology, neurochemistry, biophysical properties, projections and connectivity. The discovery of the presence of multiple neuro...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVE:After MI pathological LV remodeling is one of the major causes of death. We previously showed the NO mediated beneficial effects of nebivolol in rat MI model, in this study we aimed to evaluate the NOS related mechanisms in this phenomenon. MATERIALS AND METHODS:Rats were divided into four groups: sham opera...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:To explore the expression and function of insulin-like growth factor II (IGFII) mRNA binding protein (IMP3) in the Triple Negative Breast Cancer (TNBC). MATERIALS AND METHODS:According to previously reported gene expression array, we found that IMP3 had significantly higher expression in the CD44+CD24-ESA+ c...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201805_14965
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:Synovial inflammation plays an important role in the pathogenesis of osteoarthritis (OA), and β4GalT1 has been reported to be involved in the inflammatory process. The aim of our study was to investigate the role of β4GalT1 in the progression of inflammation and analyze the association between β4GalT1 and tum...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201906_18028
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:Over-proliferation of mesangial cells is the major pathological change of mesangial proliferative glomerulonephritis (MPGN). PTEN-PI3K/AKT pathway plays a role in regulating proliferation of mesangial cells. Anti-thymocyte serum nephritis (ATSN) is a widely used animal model for studying MPGN. This study esta...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-08-01 00:00:00
abstract::Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long noncoding RNA CASC2 alleviates the growth, migration and invasion of oral squamous cell carcinoma via downregulating CDK1, by H.-B. Xing, H.-M. Qiu, ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202011_23550
更新日期:2020-11-01 00:00:00
abstract::Columella is an important facial component and provides support and projection to the tip of the nose. Columella defects may cause significant aesthetic and functional deformities. We present our case-load of 2007: 61 patients operated in that year had been carried out and concluded. Surgical techniques for the correc...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVE:To explore a new approach for treating renal insufficiency with gene therapy by implanting decorin (DCN)-expressing fibroblasts within the renal tissue of rats with renal failure to neutralize TGF-β1 activity. MATERIALS AND METHODS:The 5/6 kidney of the selected male SD rats were removed under aseptic condit...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201804_14843
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:To determine alterations of vitamin D and parathyroid hormone levels and their relationship to insulin resistance among a sample of healthy young adult obese Saudis and to identify factors that might predict these alterations. METHODS:Age and gender matched obese young (aged 18-25 years) adult Saudis (N = 7...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2011-02-01 00:00:00
abstract:AIMS:The objective of this project was to improve the effect of EPC autograft transplantation and observe the tolerance of EPCs to I/R injury affected by metoprolol and small intestine RNA. MATERIALS AND METHODS:We isolated bone marrow-derived EPCs and examined the effects of metoprolol and small intestine RNA on EPCs...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effect of leptin (Lep) on the proliferation, invasion and apoptosis of prostate cancer cells through the extracellular regulated protein kinase 1/2 (ERK1/2) signaling pathway. MATERIALS AND METHODS:Prostate cancer DU145 cells in the logarithmic growth phase were r...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202008_22630
更新日期:2020-08-01 00:00:00
abstract::The authors evaluated the efficacy of an electronic treatment for pain, using an ion flow generator, BE-101 model by Bio-Ejt, on 19 patients suffering from acute pain of an arthrorheumatic nature. Each patient was treated for two weeks every other day (6 sittings), each sitting lasting 20 minutes at an intensity of ab...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVE:Spinal cord injury (SCI) patients had major trauma during surgery, which thus necessitates optimal choice of anesthesia drugs. The specific selection of anesthesia agents may affect body immune system. Therefore, this study aims to investigate the anesthesia effect of propofol and dexmedetomidine on the rat S...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:The study aims to construct a multi-gene risk scoring model that can be used to predict the prognosis of patients with lung squamous cell carcinoma (LUSC). PATIENTS AND METHODS:RNA-seq data from 494 LUSC tumor samples and 49 peripheral lung tissue samples were obtained from TCGA_LUSC database. Differential a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21330
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:In the clinic, therapeutic options for pulmonary arterial hypertension are limited; therefore, investigating the therapeutic strategies and novel therapies is critical for pulmonary arterial hypertension (PAH) treatment. This study aimed to evaluate the role of miRNA-126 (miR-126) and its associated signaling...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201904_17588
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVE:The pathogenesis of sinonasal polyps has not been known completely. We investigated the role of endothelial Nitric Oxide Synthase (eNOS) in the pathogenesis of sinonasal polyps. PATIENTS AND METHODS:Study group (Groups 1-3) consisted of nasal polyp samples of patients with sinonasal polyps; and control group...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the associations between obstructive sleep apnea hypopnea syndrome (OSAHS) complicated by cerebral infarction and intestinal flora, inflammatory factors, homocysteine, and adiponectin expression. PATIENTS AND METHODS:A total of 30 healthy volunteers (control group), 2...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24204
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To investigate the correlations of serum vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) levels with colorectal liver metastasis (CLM) in patients with colorectal cancer (CRC), and determine the effects of transhepatic arterial chemoembolization (TACE) on their expressions. P...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201806_15161
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:1,1-dimethylbiguanide hydrochloride (biguanide metformin) is a hypoglycemic agent that is widely used in the treatment of Type 2 diabetes. Use of metformin was found to be associated with the lower risk of cancer. It is suggested that metformin has an anticancer and antiproliferative effect and affects the a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:This study aims to evaluate the effect of trans-resveratrol/carboxymethylated (1.3/1.6)-β-d-glucan administered via nasal, after FESS, assessing nasal respiratory distress and nasal mucosa healing. PATIENTS AND METHODS:We enrolled 70 patients, from March 2019 to February 2020, with chronic nasal obstruction ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24034
更新日期:2020-12-01 00:00:00