Serotonin and forskolin increase an inwardly rectifying potassium conductance in cultured identified Aplysia neurons.

Abstract:

:1. The effect of serotonin (5-HT) and forskolin on an inwardly rectifying K+ conductance (IKR) was studied using voltage-clamp techniques in several identified Aplysia neurons isolated and maintained in primary cell culture. 2. Inward rectification was observed in the current-voltage relationship of the identified neurons R15, R2, B1, and B2 and was predominately due to IKR, as demonstrated by the dependence of inward rectification on the extracellular K+ concentration, instantaneous kinetics of the membrane current response to hyperpolarizing voltage clamp pulses, and voltage-dependent Ba2+ block of the inwardly rectifying current. 3. 5-HT increased IKR conductance between 100 and 400% in the identified neuron R15 in culture and increased IKR conductance approximately 50% in the identified neurons B1, B2, and R2 in culture. The adenylate cyclase activator, forskolin, plus a phosphodiesterase inhibitor, Ro 20-1724, also increased IKR conductance in these neurons. 4. 5-HT and forskolin modulated other ion conductances as well in all of these cultured neurons.

journal_name

J Neurophysiol

authors

Lotshaw DP,Levitan IB

doi

10.1152/jn.1987.58.5.909

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

909-21

issue

5

eissn

0022-3077

issn

1522-1598

journal_volume

58

pub_type

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