Abstract:
:Current and voltage-clamp recordings were made at room temperature from cultured mouse spinal neurons using conventional two-electrode voltage-clamp techniques and electrodes filled with either 3 M KCl, 3 M CsCl, or 3 M Cs2SO4. In the presence of tetraethylammonium and tetrodotoxin, "fast" (rapidly rising and falling) action potentials (FAP) of variable duration were recorded in most neurons. "Slow" (slowly rising and falling) depolarizing potentials (SDP) occurred in 23% of the cells, when using KCl-filled electrodes, and in 82% of the cells with CsCl-filled electrodes. The SDP was frequently preceded by an FAP, although in some cells activation of the SDP occurred before the FAP threshold was reached and in a graded fashion. Both the FAP and SDP were abolished by Cd2+ and other Ca2+ antagonists. In cells exhibiting SDPs, voltage-clamp analysis revealed a sustained (noninactivating) inward current (Isin) during depolarizing steps to potentials more positive than -45 mV. Repolarizing steps resulted in slowly decaying inward tail currents (Itail). Both Isin and Itail were abolished in solutions nominally free of Cao2+, or containing Ca2+-channel antagonists. Bao2+ did not support Isin. The data indicated a U-shaped activation curve for Isin, peaking at about -10 mV. Activation of Isin occurred exponentially with a time constant of approximately 140 ms at -23 mV, becoming faster at more depolarized potentials (ca. 50 ms at -2 mV). Deactivation was slow, giving rise to tail currents lasting seconds. In some cases deactivation could be described by a single exponential process, although frequently the kinetics were more complex. Deactivation was faster at hyperpolarized potentials and sensitive to extracellular ([Ca2+]o), duration of activating voltage steps, and the degree of activation of Isin. Using CsCl-filled electrodes, the reversal potential (Erev) for Isin was -1.7 mV (SEM 3.5 mV, n = 20). Erev always corresponded to the reversal potential for gamma-aminobutyric acid-evoked currents in the same cell. In experiments in which Cs2SO4-filled electrodes were used, Erev was estimated to be -44 mV (SEM 2.3 mV, n = 9). Neither complete substitution of Nao+ with choline ions nor elevation of [K+]o 10-fold significantly affected the estimated Erev. However, substitution of Cl0- with isethionate or methanesulphonate increased the amplitude of inward currents (recorded with CsCl-filled electrodes) and shifted Erev to more depolarized potentials. The results indicate that Cl- are the primary charge carriers for this current and that Cai2+ is required for its activation, leading us to identify it as ICl(Ca).(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Owen DG,Segal M,Barker JLdoi
10.1152/jn.1986.55.6.1115subject
Has Abstractpub_date
1986-06-01 00:00:00pages
1115-35issue
6eissn
0022-3077issn
1522-1598journal_volume
55pub_type
杂志文章abstract::Substance P (SP) hyperpolarizes approximately 80% of ferret vagal sensory neurons (nodose ganglion neurons) via NK-1 receptor-mediated activation of a potassium current (IK). A depolarizing current activated by membrane hyperpolarization could minimize the SP-induced hyperpolarization. Such a current exists in 65% of ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.2.769
更新日期:1998-02-01 00:00:00
abstract::Functional magnetic resonance imaging (fMRI) is a noninvasive tool used to probe cognitive and affective processes. Although fMRI provides indirect measures of neural activity, the advent of fMRI has allowed for1) the corroboration of significant animal findings in the human brain, and2) the expansion of models to inc...
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pub_type: 杂志文章,评审
doi:10.1152/jn.00333.2015
更新日期:2016-03-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00539.2014
更新日期:2015-01-15 00:00:00
abstract::The synaptic plasticity literature has focused on establishing necessity and sufficiency as two essential and distinct features in causally relating a signaling molecule to plasticity induction, an approach that has been surprisingly lacking in the intrinsic plasticity literature. In this study, we complemented the re...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00833.2014
更新日期:2015-04-01 00:00:00
abstract::Attenuation of paired-pulse facilitation associated with synaptic potentiation mediated by postsynaptic mechanisms. J. Neurophysiol. 78: 2707-2716, 1997. The relationship between paired-pulse facilitation (PPF) and synaptic potentiation induced by various protocols and their cellular and molecular mechanisms were exam...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.5.2707
更新日期:1997-11-01 00:00:00
abstract::AMPA-type glutamate receptors are normally Ca(2+) impermeable due to the expression of the GluR2 receptor subunit. By using GluR2 null mice we were able to detect miniature synaptic Ca(2+) transients (MSCTs) associated with AMPA-type receptor-mediated miniature synaptic currents at single synapses in primary cortical ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.88.1.29
更新日期:2002-07-01 00:00:00
abstract::1. Intracellular recordings from CA1 pyramidal neurons in the rat hippocampal slice have been used to study synaptic transmission after maximal orthodromic stimulation of the Schaffer collateral-commissural fibers. Paired-pulse stimulation was used to investigate how the first (conditioning) stimulation influenced the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.66.5.1704
更新日期:1991-11-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.3.631
更新日期:1976-05-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.6.1911
更新日期:1987-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01177.2003
更新日期:2004-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00670.2011
更新日期:2012-08-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00123.2011
更新日期:2011-08-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.4.1611
更新日期:1994-10-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00086.2013
更新日期:2013-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00171.2003
更新日期:2004-01-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.3.1367
更新日期:1995-09-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00751.2010
更新日期:2011-07-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.3.618
更新日期:1983-09-01 00:00:00
abstract::Spinothalamic (ST) neurons in the C8-T5 segments of the spinal cord were examined for responses to electrical stimulation of the left thoracic vagus nerve (LTV). Seventy-one ST neurons were studied in 39 anesthetized monkeys (Macaca fascicularis). Each neuron could be excited by manipulation of its somatic field and b...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.4.926
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1152/jn.1995.73.2.916
更新日期:1995-02-01 00:00:00
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更新日期:2006-11-01 00:00:00
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更新日期:2007-11-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00446.2009
更新日期:2009-10-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.2.496
更新日期:1987-02-01 00:00:00
abstract::The stress hormone corticosterone increases the amplitude of the slow afterhyperpolarization (sAHP) in CA1 pyramidal neurons, without affecting resting membrane potential, input resistance, or action potential characteristics. We here examined how corticosterone affects these properties in the basolateral amygdala (BL...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01137.2007
更新日期:2008-02-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.1.171
更新日期:1996-01-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1989.62.6.1360
更新日期:1989-12-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
abstract::1. Single-unit electrical activity has been recorded from 34 dorsal horn neurons in the sacral segments (S1-2) of the spinal cord in halothane-anesthetized rats. All of the neurons had cutaneous receptive fields (RFs) on the rat's tail. The neurons were classified according to their responses to both innocuous and nox...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.63.5.1022
更新日期:1990-05-01 00:00:00
abstract::The spinal network coordinating locomotion is comprised of a core of glutamate and glycine interneurons. This network is modulated by several transmitter systems including spinal GABA interneurons. The purpose of this study is to explore the contribution of GABAergic neurons to the regulation of locomotor burst freque...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00233.2004
更新日期:2004-10-01 00:00:00