Abstract:
:In many neurons more than one peptide is colocalized with a classical neurotransmitter. The functional consequence of such an arrangement has been rarely investigated. Here, within the feeding circuit of Aplysia, we investigate at a single synapse the actions of two modulatory neuropeptides that are present in a cholinergic interneuron. In combination with previous work, our study shows that the command-like neuron for feeding, CBI-2, contains two neuropeptides, feeding circuit activating peptide (FCAP) and cerebral peptide 2 (CP2). Previous studies showed that high-frequency prestimulation or repeated stimulation of CBI-2 increases the size of CBI-2 to B61/62 excitatory postsynaptic potentials (EPSPs) and shortens the latency of firing of neuron B61/62 in response to CBI-2 stimulation. We find that both FCAP and CP2 mimic these two effects. The variance method of quantal analysis indicates that FCAP increases the calculated quantal size (q) and CP2 increases the calculated quantal content (m) of EPSPs. Since the PSP amplitude represents the product of q and m, the joint action of the two peptides is expected to be cooperative. This observation suggests a possible functional implication for multiple neuropeptides colocalized with a classical neurotransmitter in one neuron.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Koh HY,Vilim FS,Jing J,Weiss KRdoi
10.1152/jn.00358.2003subject
Has Abstractpub_date
2003-09-01 00:00:00pages
2074-9issue
3eissn
0022-3077issn
1522-1598pii
00358.2003journal_volume
90pub_type
杂志文章abstract::1. The small cardioactive peptides (SCPs) are potent modulatory neuropeptides in Aplysia. Buccal ganglia neurons B1 and B2 are the largest neurons that exhibit SCP-like immunoreactivity. High-pressure liquid chromatography (HPLC)-bioassay and in vivo radiolabeling procedures confirm that these neurons contain and synt...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.59.5.1613
更新日期:1988-05-01 00:00:00
abstract::Extracellular discharges from single neurons in the internal segment of the globus pallidus (GPi) were recorded and analyzed for rate changes associated with visually guided forearm rotations to four different targets. We sought to examine how GPi neurons contribute to movement preparation and execution. Unit discharg...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.85.2.998
更新日期:2001-02-01 00:00:00
abstract::Cellular mechanisms underlying defense reactions induced by noxious heat and acids were studied in frogs (Rana pipiens) by measuring whole cell membrane currents in cultured dorsal root ganglion (DRG) neurons. Seventy-eight of 82 DRG neurons exposed to 3-s ramps of increasing temperature to 48 degrees C exhibited an i...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.88.4.1843
更新日期:2002-10-01 00:00:00
abstract::Sciatic nerve section (axotomy) increases the excitability of rat dorsal root ganglion (DRG) neurons. The changes in Ca2+ currents, K+ currents, Ca2+ sensitive K+ current, and hyperpolarization-activated cation current (I(H)) that may be associated with this effect were examined by whole cell recording. Axotomy affect...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.85.2.644
更新日期:2001-02-01 00:00:00
abstract::The brain stem nucleus locus coeruleus (LC) is thought to modulate cortical excitability by norepinephrine (NE) release in LC forebrain targets. The effects of LC burst discharge, typically evoked by a strong excitatory input, on cortical ongoing activity are poorly understood. To address this question, we combined di...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00920.2013
更新日期:2014-06-15 00:00:00
abstract::1. The effects of dopamine on the slow Ca(2+)-dependent K+ current (IAHP; AHP, afterhyperpolarization) and spike frequency adaptation were studied by whole cell voltage-clamp and sharp microelectrode current-clamp recordings in rat CA1 pyramidal neurons in rat hippocampal slices. 2. Dopamine suppressed IAHP in a dose-...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.6.2749
更新日期:1995-12-01 00:00:00
abstract::Rats typically employ 4- to 12-Hz "whisking" movements of their vibrissae during tactile exploration. The intentional sampling of signals in this frequency range suggests that neural processing of tactile information may be differentially engaged in this bandwidth. We examined action potential responses in rat primary...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01158.2002
更新日期:2003-09-01 00:00:00
abstract::Mechanosensory neurons exhibit a wide range of dynamic changes in response, including rapid and slow adaptation. In addition to mechanical factors, electrical processes may also contribute to sensory adaptation. We have investigated adaptation of afferent neurons in the stick insect femoral chordotonal organ (fCO). Th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00339.2002
更新日期:2002-11-01 00:00:00
abstract::We present a cell-based model of the Limulus lateral eye that computes the eye's input to the brain in response to any specified scene. Based on the results of extensive physiological studies, the model simulates the optical sampling of visual space by the array of retinal receptors (ommatidia), the transduction of li...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.80.4.1800
更新日期:1998-10-01 00:00:00
abstract::Parallel recording of neuronal activity in the behaving animal is a prerequisite for our understanding of neuronal representation and storage of information. Here we describe the development of micro-machined silicon microelectrode arrays for unit and local field recordings. The two-dimensional probes with 96 or 64 re...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00116.2003
更新日期:2003-08-01 00:00:00
abstract::Bis(7)-tacrine is a potent acetylcholinesterase inhibitor in which two tacrine molecules are linked by a heptylene chain. We tested the effects of bis(7)-tacrine on the spontaneous synaptic activity. Miniature endplate potentials (MEPPs) were recorded extracellularly on slices of electric organ of Torpedo marmorata. B...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.86.1.183
更新日期:2001-07-01 00:00:00
abstract::Seizures may cause brain damage due to mechanisms initiated by excessive excitatory synaptic transmission. One such mechanism is the activation of death-promoting intracellular cascades by the influx and the perturbed homeostasis of Ca2+. The neuroprotective effects of preventing the entry of Ca2+ from voltage-depende...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.6.3054
更新日期:1999-06-01 00:00:00
abstract::The nucleus of the solitary tract (NST) and the parabrachial nuclei (PbN) are the first and second relays in the rodent central taste pathway. A series of electrophysiological experiments revealed that spontaneous and taste-evoked activities of brain stem gustatory neurons are altered by descending input from multiple...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00362.2014
更新日期:2015-06-01 00:00:00
abstract::Interneuron precursors transplanted into visual cortex induce network plasticity during their heterochronic maturation. Such plasticity can have a significant impact on the function of the animal and is normally present only during a brief critical period in early postnatal development. Elucidating the synaptic and ph...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00096.2017
更新日期:2017-07-01 00:00:00
abstract::Both N-methyl-D-aspartate (NMDA) and quisqualate/AMPA-insensitive metabotropic glutamate (mGlu) receptors mediate plasticity induction in neocortex, but their interlaminar distribution in cortical microcircuits is largely unknown. We used (+)(3)H-MK801 and (3)H-glutamate binding plus saturating concentrations of NMDA,...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.4.1881
更新日期:2000-10-01 00:00:00
abstract::The pacemaker current of a bursting neuron of Helix pomatia was investigated using voltage-clamp and pressure-injection techniques. In the steady state the net membrane current was zero near threshold of the action potential at -45 mV. Negative to this potential the membrane current was inward and steady. During burst...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1985.54.6.1430
更新日期:1985-12-01 00:00:00
abstract::1. The discharge of antidromically identified medial rectus and abducens motoneurons was recorded in restrained unanesthesized goldfish during spontaneous eye movements and in response to vestibular and optokinetic stimulation. 2. All medial rectus and abducens motoneurons exhibited a similar discharge pattern. A burs...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.66.6.2125
更新日期:1991-12-01 00:00:00
abstract::Variations in cortical oscillations in the alpha (7-14 Hz) and beta (15-29 Hz) range have been correlated with attention, working memory, and stimulus detection. The mu rhythm recorded with magnetoencephalography (MEG) is a prominent oscillation generated by Rolandic cortex containing alpha and beta bands. Despite its...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00535.2009
更新日期:2009-12-01 00:00:00
abstract::Neural activity was recorded in the parietooccipital cortex while monkeys performed different tasks aimed at investigating visuomotor interactions of retinal, eye, and arm-related signals on neural activity. The tasks were arm reaching 1) to foveated targets; 2) to extrafoveal targets, with constant eye position; 3) w...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.4.2374
更新日期:2000-04-01 00:00:00
abstract::The supratrigeminal region (SupV) receives abundant orofacial sensory inputs and descending inputs from the cortical masticatory area and contains premotor neurons that target the trigeminal motor nucleus (MoV). Thus it is possible that the SupV is involved in controlling jaw muscle activity via sensory inputs during ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01145.2007
更新日期:2008-10-01 00:00:00
abstract::Second-order vestibular neurons (2 degrees VN) were identified in the isolated frog brain by the presence of monosynaptic excitatory postsynaptic potentials (EPSPs) after separate electrical stimulation of individual vestibular nerve branches. Combinations of one macular and the three semicircular canal nerve branches...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00370.2002
更新日期:2002-11-01 00:00:00
abstract::One-hertz repetitive transcranial magnetic stimulation (1Hz-rTMS) over ipsilateral motor cortex is able to modify up to 30 min the motor performance of repetitive finger opposition movements paced with a metronome at 2 Hz. We investigated whether the long-lasting rTMS effect on motor behavior can be modulated by subse...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.90428.2008
更新日期:2009-03-01 00:00:00
abstract::Transcranial direct current stimulation (tDCS) can produce a lasting polarity-specific modulation of cortical excitability in the brain, and it is increasingly used in experimental and clinical settings. Recent studies suggest that the after-effects of tDCS are related to molecular mechanisms of activity-dependent syn...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00319.2011
更新日期:2012-04-01 00:00:00
abstract::During an inspiration the output of hypoglossal (XII) motoneurons (HMs) in vitro is characterized by synchronous oscillatory firing in the 20- to 40-Hz range. To maintain synchronicity it is important that the cells fire with high reliability and precision. It is not known whether the intrinsic properties of HMs are t...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01037.2007
更新日期:2008-06-01 00:00:00
abstract::The noradrenergic system is widely thought to be important for associative learning in the olfactory system through actions in the first processing structure, the main olfactory bulb (MOB). Here, we used extracellular local field potential (LFP) and patch-clamp recordings in rat MOB slices to examine norepinephrine (N...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01324.2007
更新日期:2008-04-01 00:00:00
abstract::How does the motor system choose the speed for any given movement? Many current models assume a process that finds the optimal balance between the costs of moving fast and the rewards of achieving the goal. Here, we show that such models also need to take into account a prior representation of preferred movement speed...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00522.2013
更新日期:2014-01-01 00:00:00
abstract::We previously showed that, when observers watch an actor performing a predictable block-stacking task, the coordination between the observer's gaze and the actor's hand is similar to the coordination between the actor's gaze and hand. Both the observer and the actor direct gaze to forthcoming grasp and block landing s...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00227.2006
更新日期:2006-09-01 00:00:00
abstract::Compression apparel is popular in both medical and sport performance settings. Perceived benefits are suggested to include changes in sensory feedback transmission caused by activation of mechanoreceptors. However, little is known about effects of compression apparel on sensorimotor control. Our purpose was to mechani...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00581.2017
更新日期:2018-07-01 00:00:00
abstract::The caudal primate prefrontal cortex (PFC) is involved in target selection and visually guided saccades through both covert attention and overt orienting eye movements. Unilateral damage to the caudal PFC often leads to decreased awareness of a contralesional target alone, referred to as "neglect," or when it is prese...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00078.2019
更新日期:2019-08-01 00:00:00
abstract::1. The question of whether active uptake limits the duration of action of the inhibitory transmitter glycine has been addressed in vivo at inhibitory synapses on the goldfish Mauthner (M) cell. The kinetics of inhibitory postsynaptic potentials and inhibitory postsynaptic currents (IPSCs) evoked antidromically and by ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.4.1738
更新日期:1996-04-01 00:00:00