Two neuropeptides colocalized in a command-like neuron use distinct mechanisms to enhance its fast synaptic connection.

Abstract:

:In many neurons more than one peptide is colocalized with a classical neurotransmitter. The functional consequence of such an arrangement has been rarely investigated. Here, within the feeding circuit of Aplysia, we investigate at a single synapse the actions of two modulatory neuropeptides that are present in a cholinergic interneuron. In combination with previous work, our study shows that the command-like neuron for feeding, CBI-2, contains two neuropeptides, feeding circuit activating peptide (FCAP) and cerebral peptide 2 (CP2). Previous studies showed that high-frequency prestimulation or repeated stimulation of CBI-2 increases the size of CBI-2 to B61/62 excitatory postsynaptic potentials (EPSPs) and shortens the latency of firing of neuron B61/62 in response to CBI-2 stimulation. We find that both FCAP and CP2 mimic these two effects. The variance method of quantal analysis indicates that FCAP increases the calculated quantal size (q) and CP2 increases the calculated quantal content (m) of EPSPs. Since the PSP amplitude represents the product of q and m, the joint action of the two peptides is expected to be cooperative. This observation suggests a possible functional implication for multiple neuropeptides colocalized with a classical neurotransmitter in one neuron.

journal_name

J Neurophysiol

authors

Koh HY,Vilim FS,Jing J,Weiss KR

doi

10.1152/jn.00358.2003

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

2074-9

issue

3

eissn

0022-3077

issn

1522-1598

pii

00358.2003

journal_volume

90

pub_type

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