A fine-scale phylogenetic analysis of free-living Burkholderia species in sugarcane field soil.

abstract：:Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still con...

journal_title：Microbes and environments

authors： Urashima Y,Sonoda T,Fujita Y,Uragami A

更新日期：2012-01-01 00:00:00

abstract：:A newly-developed molecular method, hierarchical oligonucleotide primer extension (HOPE), was used to analyze various groups within the species Cylindrospermopsis raciborskii. PCR-amplified internally transcribed spacer sequences of 16S-23S from C. raciborskii in reservoir samples of Taiwan and Kinmen were examined. O...

journal_title：Microbes and environments

authors： Wu JH,Hsu MH,Hung CH,Tseng IC,Lin TF

更新日期：2010-01-01 00:00:00

abstract：:The effects of a nitrogen-fixing tree, black locust (Robinia pseudoacacia), on the distribution of bacterial species were examined in a Japanese black pine (Pinus thunbergii) and black locust-dominated area. DNA was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a Japanese black ...

journal_title：Microbes and environments

authors： Taniguchi T,Kataoka R,Tamai S,Yamanaka N,Futai K

更新日期：2009-01-01 00:00:00

abstract：:Symbioses have played pivotal roles in biological, ecological, and evolutionary diversification. Symbiotic bacteria affect the biology of hosts in a number of ways. Esteya vermicola, an endoparasitic nematophagous fungus, has high infectivity in the pine wood nematode (PWN), which causes devastating ecological damage ...

journal_title：Microbes and environments

authors： Wang R,Dong L,Chen Y,Qu L,Wang Q,Zhang Y

更新日期：2017-09-27 00:00:00

abstract：:Antibiotics in animal husbandry are used to maintain welfare, but lead to the generation of resistant strains. We analyzed commensal multidrug-resistant Escherichia coli from pigs at the beginning and end of the production cycle in a farm with a farrow-to-finish system in order to investigate whether clonal spread or ...

journal_title：Microbes and environments

authors： Mazurek J,Bok E,Baldy-Chudzik K

更新日期：2018-09-29 00:00:00

abstract：:Effects of different land uses on densities of Escherichia coli, enterococci, and Clostridium perfringens in the water and soil of a tropical watershed were investigated. Densities of fecal indicator bacteria (FIBs) in the watershed exhibited a clear land-use dependency in the stream water. Significantly higher concen...

journal_title：Microbes and environments

authors： Goto DK,Yan T

更新日期：2011-01-01 00:00:00

abstract：:Members of the Marseilleviridae family are large DNA viruses with icosahedral particle structures that infect Acanthamoeba cells. The first Marseillevirus to be discovered was isolated in 2009. Since then, several other members of the Marseilleviridae family have been reported, including Lausannevirus, Senegalvirus, C...

journal_title：Microbes and environments

authors： Takemura M

更新日期：2016-12-23 00:00:00

abstract：:Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Ki...

journal_title：Microbes and environments

authors： Evans JS,López-Legentil S,Erwin PM

更新日期：2018-12-28 00:00:00

abstract：:Marine fungus-like eukaryotic unicellular protists (thraustochytrids) are considered to play an important role in the marine microbial food web. However, their abundance, distribution, and relative biomass in coastal waters have not yet been examined in detail. By using a flow cytometry method (FCM) for the rapid enum...

journal_title：Microbes and environments

authors： Duan Y,Sen B,Xie N,Paterson JS,Chen Z,Wang G

更新日期：2018-07-04 00:00:00

abstract：:"Terre Calde di Medolla" (TCM) (literally, "Hot Lands of Medolla") refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO2, which has been linked to CH4 oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition of the total bacterial...

journal_title：Microbes and environments

authors： Cappelletti M,Ghezzi D,Zannoni D,Capaccioni B,Fedi S

更新日期：2016-12-23 00:00:00

abstract：:Small cyanobacteria (<2 µm, pico-cyanobacteria) are abundant in waters deeper than the oxic-anoxic zone in the halocline of a saline meromictic lake, Lake Suigetsu, Fukui, Japan. We have isolated 101 strains that were grouped into six groups by means of the phycobiliprotein composition and sequence homology of the int...

journal_title：Microbes and environments

authors： Ohki K,Yamada K,Kamiya M,Yoshikawa S

更新日期：2012-01-01 00:00:00

abstract：:The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the ...

journal_title：Microbes and environments

authors： Chien MF,Tosa S,Huang CC,Endo G

更新日期：2009-01-01 00:00:00

abstract：:Rapid and continuous pressure treatment was realized using a hydraulic pump and the momentary decompression following high pressurization was used to inactivate bacteria. The number of colony-forming E. coli decreased to 1/1000 in response to 10 cycles of pressure treatment. In groundwater samples, repeated pressure t...

journal_title：Microbes and environments

authors： Baba T,Yamaguchi N,Tani K,Nasu M

更新日期：2011-01-01 00:00:00

abstract：:Rice seedlings were inoculated with Azospirillum sp. B510 and transplanted into a paddy field. Growth in terms of tiller numbers and shoot length was significantly increased by inoculation. Principal-coordinates analysis of rice bacterial communities using the 16S rRNA gene showed no overall change from B510 inoculati...

journal_title：Microbes and environments

authors： Bao Z,Sasaki K,Okubo T,Ikeda S,Anda M,Hanzawa E,Kakizaki K,Sato T,Mitsui H,Minamisawa K

更新日期：2013-01-01 00:00:00

abstract：:In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil...

journal_title：Microbes and environments

authors： Kong B,Chen L,Kasahara Y,Sumida A,Ono K,Wild J,Nagatake A,Hatano R,Hara T

更新日期：2017-06-24 00:00:00

abstract：:Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

journal_title：Microbes and environments

authors： Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

更新日期：2014-01-01 00:00:00

abstract：:We investigated the effects of the water status (flooded or non-flooded) and presence of the nosZ gene in bradyrhizobia on the bradyrhizobial community structure in a factorial experiment that examined three temperature levels (20°C, 25°C, and 30°C) and two soil types (andosol and gray lowland soil) using microcosm in...

journal_title：Microbes and environments

authors： Saeki Y,Nakamura M,Mason MLT,Yano T,Shiro S,Sameshima-Saito R,Itakura M,Minamisawa K,Yamamoto A

更新日期：2017-06-24 00:00:00

abstract：:Giant viruses of 'Megaviridae' have the ability to widely disperse around the globe. We herein examined 'Megaviridae' communities in four distinct aquatic environments (coastal and offshore seawater, brackish water, and hot spring freshwater), which are distantly located from each other (between 74 and 1,765 km), usin...

journal_title：Microbes and environments

authors： Li Y,Endo H,Gotoh Y,Watai H,Ogawa N,Blanc-Mathieu R,Yoshida T,Ogata H

更新日期：2019-09-25 00:00:00

abstract：:The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are specific to bacteria....

journal_title：Microbes and environments

authors： Ikenaga M,Sakai M

更新日期：2014-09-17 00:00:00

abstract：:The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxi...

journal_title：Microbes and environments

authors： Yanagawa K,Tani A,Yamamoto N,Hachikubo A,Kano A,Matsumoto R,Suzuki Y

更新日期：2016-06-25 00:00:00

abstract：:Diarrhea is often associated with marked alterations in the intestinal microbiota, termed dysbiosis; however, limited information is currently available on the intestinal microbiota in captive golden snub-nosed monkeys (Rhinopithecus roxellana) with diarrhea. We herein characterized the fecal microbiota in diarrhea an...

journal_title：Microbes and environments

authors： Zhu H,Zeng D,Wang Q,Wang N,Zeng B,Niu L,Ni X

更新日期：2018-09-29 00:00:00

abstract：:Due to their obligate symbiotic nature and lack of long-term storage methods, the strain collection of arbuscular mycorrhizal (AM) fungi requires periodic proliferation using a pot culture with host plants. Therefore, a method to evaluate the purity of proliferated AM fungal cultures is critical for the quality contro...

journal_title：Microbes and environments

authors： Ohtomo R,Oka N,Morimoto S

更新日期：2019-12-27 00:00:00

abstract：:Soil and rhizosphere environments were examined in order to determine the identity and relative abundance of bacteria that catabolize d- and l-amino acids as the sole source of carbon and nitrogen. All substrates were readily catabolized by bacteria from both environments, with most d-amino acids giving similar CFU co...

journal_title：Microbes and environments

authors： Radkov AD,McNeill K,Uda K,Moe LA

更新日期：2016-06-25 00:00:00

abstract：:The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and cha...

journal_title：Microbes and environments

authors： Jabloune R,Khalil M,Ben Moussa IE,Simao-Beaunoir AM,Lerat S,Brzezinski R,Beaulieu C

更新日期：2020-01-01 00:00:00

abstract：:Nitrogen (N) cycles have been directly linked to the functional stability of ecosystems because N is an essential element for life. Furthermore, the supply of N to organisms regulates primary productivity in many natural ecosystems. Microbial communities have been shown to significantly contribute to N cycles because ...

journal_title：Microbes and environments

authors： Isobe K,Ohte N

更新日期：2014-01-01 00:00:00

abstract：:Diazotrophs had not previously been identified among bacterial species in the phylum Bacteroidetes until the rapid expansion of bacterial genome sequences, which revealed the presence of nitrogen fixation (nif) genes in this phylum. We herein determined the draft genome sequences of Bacteroides graminisolvens JCM 1509...

journal_title：Microbes and environments

authors： Inoue J,Oshima K,Suda W,Sakamoto M,Iino T,Noda S,Hongoh Y,Hattori M,Ohkuma M

更新日期：2015-01-01 00:00:00

abstract：:Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase ...

journal_title：Microbes and environments

authors： Kajiwara H,Toda M,Mine T,Nakada H,Yamamoto T

更新日期：2012-01-01 00:00:00

abstract：:The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotu...

journal_title：Microbes and environments

authors： Hashimoto S,Goto K,Pyromyou P,Songwattana P,Greetatorn T,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2020-01-01 00:00:00

abstract：:The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

journal_title：Microbes and environments

authors： Wang Y,Shi T,Huang G,Gong J

更新日期：2018-09-29 00:00:00

abstract：:Terrestrial hydrocarbon seeps are an important source of naturally emitted methane over geological time. The exact community compositions responsible for carbon cycling beneath these surface features remain obscure. As sulfate reduction represents an essential process for anoxic organic mineralization, this study coll...

journal_title：Microbes and environments

authors： Cheng TW,Lin LH,Lin YT,Song SR,Wang PL

更新日期：2014-01-01 00:00:00