Abstract：
PURPOSE:To analyze the expression of 78 kDa glucose-regulated protein (GRP78) and activating transcription factor 6 (ATF6), 2 factors in the unfolded protein response (UPR), in age-related and diabetes-associated cataract. SETTING:Universidad Autónoma de Aguascalientes, Aguascalientes, México. DESIGN:Experimental study. METHODS:The qualitative and quantitative expression of GRP78 and ATF6 were measured in surgical samples from 11 senile cataracts, 9 diabetic-associated cataracts, and 3 normal lenses. Both proteins were detected by immunofluorescence and immunogold-conjugated antibodies. Quantitative morphometry was used to analyze the differences in GRP78 and ATF6 between samples. The Mann-Whitney test was used for statistical analysis. RESULTS:Scanning electron microscopy showed the characteristic organization of fibers in normal lenses with regular alignment and interdigitation between them. On the other hand, lenses from eyes with senile or diabetic cataract showed the same pattern of misalignment and disorganization of the fibers. Both proteins were detected through immunofluorescence in senile and diabetic cataracts, but not in normal lenses. Immunogold-conjugated antibodies and transmission electron microscopy showed that GRP78 and ATF6 grains were 30% higher and 35% higher, respectively, in diabetic cataracts than in senile cataracts (P<.05). CONCLUSIONS:These data show for the first time in humans that GRP78 and ATF6 are present in lens fibers of senile cataracts and diabetic cataracts, establishing that the UPR may be important in the process of cataractogenesis. FINANCIAL DISCLOSURE:No author has a financial or proprietary interest in any material or method mentioned.
journal_name
J Cataract Refract Surgjournal_title
Journal of cataract and refractive surgeryauthors
Torres-Bernal BE,Torres-Bernal LF,Gutiérrez-Campos RR,Kershenobich Stalnikowitz DD,Barba-Gallardo LF,Chayet AA,Ventura-Juárez Jdoi
10.1016/j.jcrs.2014.02.038subject
Has Abstractpub_date
2014-10-01 00:00:00pages
1697-705issue
10eissn
0886-3350issn
1873-4502pii
S0886-3350(14)01007-4journal_volume
40pub_type
杂志文章abstract：PURPOSE:To evaluate the long-term evolution of refractive error changes in eyes of children who have primary intraocular lens (IOL) implantation to allow more accurate prediction of what IOL power should be implanted at a given age. SETTING:Department of Ophthalmology, Indiana University School of Medicine, Indianapol...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(01)01156-7
更新日期：2002-05-01 00:00:00
abstract：:We used ultrasound to measure axial length in 66 eyes before and three months after extracapsular cataract extraction with implantation of a posterior chamber lens. An ultrasound velocity of 2,718 m/s for poly(methyl methacrylate) was assumed. The intraindividually measured difference (postoperative--preoperative axia...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(89)80035-5
更新日期：1989-11-01 00:00:00
abstract：PURPOSE:To determine the reproducibility of morphoscopic contrast sensitivity test values in healthy subjects using the Visual Capacity Analyzer (VCA) (L2 Informatique). SETTING:Institute of Vision Research, Department of Ophthalmology, Yong-Dong Severance Hospital, Yonsei University, Seoul, South Korea. METHODS:Five...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(03)00044-0
更新日期：2003-09-01 00:00:00
abstract：:Two hundred consecutive patients were enrolled in a randomized, prospective clinical trial evaluating the efficacy of diclofenac sodium (Voltaren Ophthalmic) in reducing patient discomfort after cataract surgery. Other factors evaluated were the effect of preoperative flurbiprofen (Ocufen) in preventing intraoperative...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0886-3350(13)80508-1
更新日期：1995-03-01 00:00:00
abstract：PURPOSE:To compare the long-term results of combined extracapsular cataract extraction (ECCE), intraocular lens (IOL) implantation, and trabeculectomy with those of trabeculectomy alone in low-risk patients. SETTING:An ophthalmic department in a British general district hospital. METHODS:We did a retrospective analys...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(96)80249-5
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:To devise a method for measuring excimer laser photorefractive keratectomy (PRK) ablation zone decentration. SETTING:Excimer Laser Clinic, Sunderland Eye Infirmary, Sunderland Tyne and Wear, England. METHODS:Fifty-three eyes of 53 consecutive patients having PRK for a spherical myopic refractive error were st...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(96)80239-2
更新日期：1996-04-01 00:00:00
abstract：:We describe a variation of stop and chop phacoemulsification. During chopping the chopper's vertical element is depressed posteriorly toward the optic nerve and is placed well within the capsulorhexis margin, leading to in situ chopping. Each fragment is continuously chopped, and the small pieces are stuffed inside th...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章,评审
doi：10.1016/s0886-3350(96)80003-4
更新日期：1996-06-01 00:00:00
abstract：:Fifty-one patients available for a 2 1/2 year follow-up after intracapsular cataract extraction and implantation of the semiflexible McGhan/3M, style 70 anterior chamber lens were evaluated for intraocular pressure (IOP). No significant change in the IOP could be demonstrated despite progression of iris-like tissue ad...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(87)80113-x
更新日期：1987-09-01 00:00:00
abstract：:We present a case of spontaneous globe luxation and wound dehiscence that developed in a patient with proptosis 1 day after phacoemulsification with posterior chamber intraocular lens implantation. The wound was repaired and a lateral tarsorrhaphy was performed with successful prevention of further luxations. Upon cat...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(03)00056-7
更新日期：2003-09-01 00:00:00
abstract：:A 77-year-old woman had uneventful phacoemulsification. Postoperative examination revealed that chloramphenicol ointment instilled in the conjunctival fornix had entered the anterior chamber and smeared the intraocular lens (IOL). Immediate surgical removal of the ointment with IOL exchange was performed. The eye sett...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(02)01503-1
更新日期：2003-03-01 00:00:00
abstract：PURPOSE:To assess the safety, efficacy, stability, and predictability of collagen copolymer toric phakic intraocular lens (pIOL) implantation to correct myopia and astigmatism in eyes with keratoconus. SETTING:Fernández-Vega Ophthalmological Institute, Oviedo, Spain. METHODS:This prospective study comprised keratocon...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2009.11.032
更新日期：2010-06-01 00:00:00
abstract：:We analyzed an enucleated eye that was blind and painful from a 66-year-old patient implanted with a Tennant modification of the Choyce Mark VIII anterior chamber intraocular lens (IOL) as a secondary procedure in 1978. The eye developed glaucoma, with implantation of an Ahmed valve in 2006. Gross and light microscopi...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2011.10.023
更新日期：2012-02-01 00:00:00
abstract：:We used the finite element method to evaluate different capsulotomy techniques. Nucleus expression was simulated in a computer model to visualize stress distribution in the anterior capsule. The finite element model demonstrated that the capsulorhexis edge is strong because of low and uniform stress distribution in th...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(13)80176-9
更新日期：1994-07-01 00:00:00
abstract：PURPOSE:Because the optical performance of eyes implanted with multifocal intraocular lenses will theoretically depend upon the size and position of the pupil relative to the near and distance zones of the lens, we designed a study to determine whether preoperative pupil size is useful in predicting the postoperative p...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(96)80013-7
更新日期：1996-06-01 00:00:00
abstract：:Descemet-stripping automated endothelial keratoplasty (DSAEK) is an alternative to penetrating keratoplasty for the treatment of corneal endothelial disease that involves stripping diseased Descemet membrane (DM) from the recipient. Descemet membrane stripping is currently performed with a generic balanced salt soluti...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2008.01.037
更新日期：2008-06-01 00:00:00
abstract：:I describe an intraoperative method for the consistent anchoring of the intraocular lens (IOL) and cataract surgery and on the patient's visual axis using coaxial microscope optics, surgeon-guided patient fixation, the precision pulse capsulotomy (PPC) device (Zepto) and utilizing the first (and fourth) Purkinje image...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2018.02.013
更新日期：2018-05-01 00:00:00
abstract：PURPOSE:To evaluate the results of the first blue light-filtering photochromic intraocular lens (IOL) and compare them with those of a regular yellow blue light-filtering IOL and a clear ultraviolet-filtering IOL in human eyes under various lighting conditions. SETTING:Beijing Tongren Eye Center, Beijing Tongren Hospi...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jcrs.2010.07.024
更新日期：2010-12-01 00:00:00
abstract：PURPOSE:To assess the effectiveness of using anterior chamber irrigation of unpreserved lidocaine 1% as anesthesia during cataract surgery. SETTING:Private group ophthalmology practice. METHODS:This study prospectively evaluated 1000 of 1012 consecutive eyes having temporal corneal incision cataract surgery to determ...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章
doi：10.1016/s0886-3350(97)80212-x
更新日期：1997-05-01 00:00:00
abstract：PURPOSE:To evaluate the protective properties of combined sodium hyaluronate 2% and mannitol 0.5% (Visiol) on the corneal endothelium in the presence of oxidative stress induced by hydrogen peroxide (H(2)O(2)). SETTING:Instituto Oftalmológico de Alicante, Universidad Miguel Hernández, Alicante, Spain. METHODS:This wa...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2004.11.055
更新日期：2005-06-01 00:00:00
abstract：:A modified version of peribulbar anesthesia is described. An analysis of 230 consecutive ophthalmic procedures including cataract surgery, penetrating keratoplasty, and scleral buckling demonstrates the safety and effectiveness of this modified peribulbar anesthesia technique. ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(88)80156-1
更新日期：1988-07-01 00:00:00
abstract：PURPOSE:To evaluate the effect of genipin, a natural crosslinker, on porcine corneas. SETTING:Department of Ophthalmology, Universidad Nacional de Colombia, Bogota, Colombia. METHODS:Corneal strips (12.0 mm x 2.3 mm) were harvested from porcine eyes and treated by incubation with genipin at concentrations of 1.00%, 0...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2009.11.003
更新日期：2010-04-01 00:00:00
abstract：PURPOSE:To demonstrate the outcome for patients after cataract extraction using the Catquest cataract questionnaire and discuss the models validity in assessing outcome. SETTING:Thirty-five Swedish departments of ophthalmology. METHODS:Patients having cataract extraction performed by surgeons from 35 Swedish departme...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/s0886-3350(98)80053-9
更新日期：1998-07-01 00:00:00
abstract：PURPOSE:To evaluate how visual acuities at different distances correlate with results from defocus curves. SETTING:Department of Ophthalmology and Institute of Medical Physics, University of Vienna, Medical School, Vienna, and Department of Ophthalmology, Krankenhaus St. Pölten, St. Pölten, Austria. METHODS:This stud...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(02)01317-2
更新日期：2002-11-01 00:00:00
abstract：PURPOSE:To report 4 cases of Pseudomonas aeruginosa endophthalmitis caused by internal contamination of the internal pathways of a phacoemulsifier. SETTING:Ophthalmology Center, Perpignan, France. METHODS:Four clinical cases of postoperative endophthalmitis occurred after phacoemulsification. An investigation was nec...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(99)80052-2
更新日期：1999-04-01 00:00:00
abstract：PURPOSE:To develop a system for high-resolution imaging of the posterior lens capsule after intraocular lens surgery for objective assessment of posterior capsule opacification (PCO). SETTING:Department of Cataract and Refractive Surgery, St. Thomas' Hospital, London, United Kingdom. METHODS:A system was developed th...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(97)80023-5
更新日期：1997-12-01 00:00:00
abstract：PURPOSE:To evaluate the relationship between the thinnest point in corneal thickness and the refractive state, keratometry, age, sex, and the ocular side. SETTING:Eye clinics in Germany and Austria and the Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. DESIGN:Cross-section...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2011.06.033
更新日期：2011-12-01 00:00:00
abstract：PURPOSE:To determine the appropriate correction of predicted intraocular lens (IOL) power for sulcus fixation and to prospectively assess the validity of the obtained correction value in patients having sulcus fixation. SETTING:Department of Ophthalmology, Saiseikai Kurihashi Hospital, Saitama, Japan. METHODS:The pos...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(03)00250-5
更新日期：2003-10-01 00:00:00
abstract：:Iris fixation of posterior chamber intraocular lenses (IOLs) is a technique that has been recommended for patients in whom a posterior chamber IOL is indicated but there is insufficient capsular support. We present 4 cases of iris-fixated posterior chamber IOLs with complications related to the procedure. Three patien...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2005.06.048
更新日期：2005-12-01 00:00:00
abstract：PURPOSE:To propose an alternative method to characterize postocclusion surge using a collapsible artificial anterior chamber to replace the currently used rigid anterior chamber model. SETTING:Fundación Oftamológica Los Andes, Santiago, Chile. METHODS:The distal end of a phacoemulsification handpiece was placed insid...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2005.03.061
更新日期：2005-10-01 00:00:00
abstract：PURPOSE:To characterize and compare the higher-order aberrations (HOAs) in 3 intraocular lenses (IOLs) designed to minimize HOAs using a new physical model eye. SETTING:Department of Ophthalmology, University of Auckland, New Zealand. METHODS:Measurements of higher-order wavefront aberrations were obtained using the ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2008.11.048
更新日期：2009-03-01 00:00:00
