Abstract:
:A non-complement-binding monoclonal antibody, TA-1, recognizing determinants on human T lymphocytes, was linked to the plant seed toxin ricin, either the intact molecule or purified ricin A chain. Peripheral blood lymphocytes were pretreated with conjugate for 2 hr, washed, and then measured in vitro for T cell proliferation. Studies showed that antibody-intact ricin conjugates were up to 39-fold more inhibitory than antibody-A-chain conjugates. Killing was selective because an unreactive control antibody linked to toxin had minimal inhibitory effect. Dose response curves obtained in human studies were nearly identical to curves obtained in an animal model n which a monoclonal anti-murine T cell antibody (anti-Thy 1.1) was linked to ricin and ricin A chain. In the human system, longer exposures of peripheral blood cells to conjugates did not alter our findings. TA-1-ricin conjugates were tested against human ALL cell lines. KOPN-1, a cell line bearing the determinant reactive with TA-1 was selectively eliminated within 2 days after pretreatment with 500 ng/ml. Even 10-fold greater concentrations of TA-1-A chain were not adequate for leukemic cell destruction. These findings (1) show for the first time in a human model that monoclonal antibodies, directed against certain differentiation antigens when linked to ricin A chain are not as effective in normal or malignant cell killing as when linked to intact ricin; (2) contribute to the growing body of evidence showing that monoclonal antibody A chain conjugates do not permit the acquisition of levels of toxin sufficient to destroy target cells; and (3) are important relative to increasing interest in use of antibody-toxin conjugates for graft-versus-host disease prophylaxis in allogeneic bone marrow transplantation.
journal_name
Transplantationjournal_title
Transplantationauthors
Vallera DA,Quinones RR,Azemove SM,Soderling CCdoi
10.1097/00007890-198404000-00015subject
Has Abstractpub_date
1984-04-01 00:00:00pages
387-92issue
4eissn
0041-1337issn
1534-6080journal_volume
37pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Previous studies have shown that posttransplant total lymphoid irradiation, anti-thymocyte globulin, and an intravenous donor blood cell infusion induce tolerance to ACI heart allografts in Lewis rat hosts. METHODS:In the current study, fresh ACI monocytes and dendritic cell precursors, derived from short-t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199811270-00004
更新日期:1998-11-27 00:00:00
abstract::The major role of anti-alphaGal antibodies in the hyperacute rejection of pig organs by humans and baboons has been clearly demonstrated. Spacered alpha-galactose disaccharide (Gal(alpha1)-3Gal) hapten was produced by chemical synthesis and covalently attached to a flexible, hydrophilic polymer (PAA), which in turn wa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199611270-00001
更新日期:1996-11-27 00:00:00
abstract:BACKGROUND:Sirolimus (SRL) seems to impair renal recovery from ischemic injury in animal models and delayed graft function after clinical renal transplantation. This study determined the impact of SRL on renal recovery after ischemia-reperfusion injury in a rat kidney transplant model. METHODS:Syngeneic kidneys were p...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000095897.38634.30
更新日期:2003-12-15 00:00:00
abstract::Since 1981, when the liver transplantation program was initiated at the University of Pittsburgh, we have been impressed with the prevalence of pancreatitis occurring following liver transplantation in patients transplanted for hepatitis B-related liver disease. To either confirm this clinical impression or refute it,...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198806000-00012
更新日期:1988-06-01 00:00:00
abstract:BACKGROUND:Clinical and epidemiological data of pandemic influenza A H1N1 infection in solid-organ transplant recipients have been described, but scarce data compare these outcomes with nonimmunocompromised patients. METHODS:We retrospectively reviewed and compared the clinical presentation, morbidity, and mortality o...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31823aa528
更新日期:2012-01-15 00:00:00
abstract:BACKGROUND:Cytotoxic IgG against class I antigens can contribute to renal dysfunction or failure after transplantation. However, the clinical relevance of IgG measured by flow cytometric cross-matching is controversial. This study correlated pre- and postoperative flow reactivity with clinical outcome among renal trans...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199802150-00029
更新日期:1998-02-15 00:00:00
abstract:BACKGROUND:Blood group-incompatible transplantation is one strategy used when a potential recipient does not have a compatible living donor. Current practice includes desensitization strategies to reduce antibody titers. However, when antibodies are low, in cardiac transplantation in neonates for example, no desensitiz...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825b7608
更新日期:2012-08-27 00:00:00
abstract:BACKGROUND:Donation after cardiac death (DCD) increases the number of donor kidneys but is associated with more primary nonfunction (PNF) and delayed graft function (DGF). It has been suggested that biomarkers in the preservation solution of machine perfused kidneys may predict PNF, although evidence is lacking. METHO...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31827908e6
更新日期:2013-02-27 00:00:00
abstract::We report on the to date largest recovery of 11 organs from a single deceased donor with the transplantation of face, bilateral upper extremities, heart, 1 lung, liver (split for 2 recipients), kidneys, pancreas, and intestine. Although logistically challenging, this case demonstrates the feasibility and safety of the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001200
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Kidney donation after cardiac death leads to vascular damage as a result of warm ischemia, affecting renovascular circulating volume. Novel ultrasound dilution techniques may be used to measure renovascular circulating volumes during hypothermic machine perfusion of donor kidneys. METHODS:Renovascular circu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828a46f7
更新日期:2013-05-15 00:00:00
abstract::We have previously shown that trophic factor supplementation (TFS) of University of Wisconsin (UW) solution enhanced kidney viability after cold storage. Here, we use an in vitro model to study the effect of TFS on early apoptotic changes after cold ischemic storage. Mitochondrial membrane potential was determined by ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000242524.35562.4b
更新日期:2007-01-15 00:00:00
abstract::We report the long-term follow-up (greater than 1 year) of 46 patients (12 pediatric) randomized to receive OKT3 for the first 14 days after OLT with low-dose steroids compared with 39 patients (8 pediatric) who received cyclosporine, steroids, and azathioprine. The mean period of follow-up for survivors was 648 +/- 2...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199107000-00019
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:Genetic variants of the renin-angiotensin system (RAS) have been implicated in the progression of native kidney diseases. A decreased long-term renal allograft function has also been associated with increased activity of RAS, which may be genetically determined. METHODS:The effect of the angiotensinogen (AG...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200203270-00023
更新日期:2002-03-27 00:00:00
abstract::Spleen cells obtained from C57BL/Ks (Ks, H-2d) mice carrying passively enhanced Sarcoma I (Sa I, H-2a) tumors were tested for alloantibody formation, lymphocyte blastogenesis, antibody-dependent cellular cytotoxicity, and cell to cell cytotoxicity. Assays were usually performed approximately 6 weeks after tumor inocul...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197803000-00011
更新日期:1978-03-01 00:00:00
abstract::We have investigated the immunosuppressive effects of thalidomide (Thal) in a bone marrow transplant (BMT) model for graft-versus-host disease (GVHD). Lewis rats received RT1-incompatible marrow transplants from ACI rats after total-body irradiation. Twenty-two of twenty-three rats with established severe acute GVHD w...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198605000-00018
更新日期:1986-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to analyze humoral xenoreactivity of various Old World primate species sera against pig islets and the effects of these sera on pig islet viability and function after culture. METHODS:Freshly isolated or cultured adult pig islets were analyzed by immunohistology or by cytofluorimet...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199812150-00012
更新日期:1998-12-15 00:00:00
abstract:BACKGROUND:After the discontinuation of the manufacturing Liberase HI because of a small potential for prion disease transmission, Roche Diagnostics (Indianapolis, IN) developed a new enzyme product (Liberase MTF [mammalian tissue free]), which is similar to Liberase HI with the exception that no mammalian tissue is us...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181e117ce
更新日期:2010-08-15 00:00:00
abstract:BACKGROUND:In this study, we investigated the effects of selective endothelin (ET) receptor antagonism during different periods of cold ischemia on glomerular and tubular function and long-term survival in renal transplantation. METHODS:Left renal transplantation was performed in Lewis rats after 2 hr of cold ischemia...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200103150-00007
更新日期:2001-03-15 00:00:00
abstract:BACKGROUND:Proteinuria is associated with endothelial dysfunction (ED) and increased mortality. We investigated whether urinary protein excretion (UPE) is correlated with markers of ED and whether these markers affect the association of proteinuria with mortality in renal transplant recipients (RTR). METHODS:Six hundr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181903d25
更新日期:2008-12-27 00:00:00
abstract:BACKGROUND:Posttransplant lymphoproliferative disease (PTLD) after pediatric liver transplantation has been associated with high mortality rates. METHODS:The present study examined 282 consecutive pediatric liver transplant recipients from October 1989 to June 1996 who received primary tacrolimus immunosuppression. Th...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199810270-00014
更新日期:1998-10-27 00:00:00
abstract:BACKGROUND:Liver transplant recipients suffer many postoperative complications. Few studies evaluated the effects of fluid management on these complications. We conducted an observational cohort study to evaluate the association between intraoperative fluid balance and postoperative acute kidney injury (AKI) and other ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002998
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:The aim of this prospective, multicenter, noncomparative, open-label trial was to evaluate the prophylactic use of caspofungin in adult liver transplant recipients at high risk of developing invasive fungal infections (IFI). METHODS:Patients received caspofungin for at least 21 days. A successful treatment o...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0b013e3181932e76
更新日期:2009-02-15 00:00:00
abstract:BACKGROUND:Inhibition of complement in small animal models of xenotransplantation has demonstrated graft infiltration with natural killer (NK) cells and monocytes associated with endothelial cell (EC) activation. We have previously demonstrated that human NK cells activate porcine EC in vitro, which results in adhesion...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199811150-00017
更新日期:1998-11-15 00:00:00
abstract:BACKGROUND:The liver plays a central role in glucose homeostasis by releasing glucose in the fasting state and by taking up and converting into glycogen part of the glucose absorbed from the gastrointestinal tract after meal ingestion. METHODS:To determine whether the hepatic denervation that accompanies liver transpl...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200003150-00052
更新日期:2000-03-15 00:00:00
abstract::Thromboxane (Tx) plays a vital role in the dysfunction and ultimate rejection of MHC-disparate renal allografts. In addition to its potent vasoconstrictory properties, in vivo studies have implied that Tx is capable of promoting immune cytotoxic T cell function within transplants. In this study, we have examined the i...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199209000-00021
更新日期:1992-09-01 00:00:00
abstract::The results presented in this report demonstrate that T cells, isolated from AGUS rats bearing long-term (WAG X AGUS)F1 spleen allografts adoptively transferred to irradiated AGUS recipients could not mediate the rejection of WAG hearts but rejected PVG. A hearts in acute fashion. Further, unresponsive T cells were ab...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198709000-00024
更新日期:1987-09-01 00:00:00
abstract::Orthotopic liver allografts from BN donors to LEW recipients are spontaneously accepted, and the recipients develop donor-specific immunological unresponsiveness. This unresponsiveness may be mediated by suppressor T cells. Immunomagnetically purified splenic T cells from LEW rats bearing BN liver grafts were shown to...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199212000-00019
更新日期:1992-12-01 00:00:00
abstract::A monoclonal anti-Lyt-1.1 serum has been characterized in terms of its effect on various cell populations involved in cell-mediated immune responses. The monoclonal serum was compared to a conventionally prepared anti-Lyt-1.1 serum. Cytotoxic T cell precursors and effectors were found to be Lyt-1.1+. Helper T cells wh...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198101000-00013
更新日期:1981-01-01 00:00:00
abstract::The optimal timing of administration of free radical scavengers in lung preservation has yet to be determined. In previous studies, the oxygen free radical scavenger dimethylthiourea (DMTU) has improved pulmonary function when added to Eurocollins flush solution at the time of lung harvest and infused at the time of l...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199208000-00003
更新日期:1992-08-01 00:00:00
abstract::Unstimulated whole saliva and serum samples were collected from ten allogeneic bone marrow transplant recipients before and after bone marrow transplantation, seven donors, and from twenty healthy individuals. Two patterns with regard to salivary IgA were found after transplantation. In five patients, a short-lasting ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198811000-00016
更新日期:1988-11-01 00:00:00