Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.

Abstract:

:In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold. In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01. Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment. Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation. Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Li M,Si S,Hao B,Zha Y,Wan C,Hong S,Kang Y,Jia J,Zhang J,Li M,Zhao C,Tu Y,Zhou S,Peng L

doi

10.1016/j.biortech.2014.07.017

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

447-454

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)00982-1

journal_volume

169

pub_type

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