Abstract:
:The fibrinolytic potential of tissue-type plasminogen activator (t-PA) either incorporated in a clot (endogenous) or added to the surrounding plasma (exogenous), was studied in an in vitro system consisting of 125I-labeled human plasma clots (200 microliters) immersed in human plasma (2 ml). Clot lysis was measured as a function of endogenous t-PA concentration (in the absence of added exogenous t-PA), as a function of exogenous t-PA concentration (without added endogenous t-PA) and as a function of the same concentration of both endogenous and exogenous t-PA. Equivalent clot lysis was obtained with a 2 to 4 times lower concentration of endogenous t-PA as compared to exogenous t-PA, corresponding to a 20 to 40 times smaller total amount of endogenous versus exogenous t-PA. Fifty percent lysis in 5 hrs was obtained with about 5 IU/ml of endogenous t-PA or with 10 IU/ml of exogenous t-PA. The presence of both exogenous (10 IU/ml) and endogenous (5 IU/ml) t-PA resulted in 50 percent lysis in 1.5 hrs, indicating that t-PA incorporated in a thrombus contributes significantly to its lysis by exogenous t-PA. Similar results were obtained with plasma obtained after 10 min of venous occlusion in seven healthy subjects. Spontaneous clot lysis within 5 hrs was only observed with post-occlusion clots in pre- or post- occlusion plasma in two subjects in whom the t-PA level rose to 10-15 IU t-PA/ml. In the five other subjects with post-occlusion t-PA levels below 2 IU/ml, no clot lysis was observed within 24 hrs. The influence of the fast-acting inhibitor of t-PA on clot lysability by endogenous or exogenous t-PA was investigated by immersing clots prepared from normal or inhibitor-rich plasma (endogenous inhibitor) in normal or inhibitor-rich plasma (exogenous inhibitor). Exogenous t-PA inhibitor efficiently neutralizes clot lysis by both exogenous and endogenous t-PA. Endogenous t-PA inhibitor, however, efficiently neutralizes endogenous t-PA but has little influence on clot lysis by exogenous t-PA. These findings indicate that t-PA inhibitor is not concentrated into a clot and that t-PA inhibitor in plasma efficiently neutralizes t-PA incorporated in a clot. alpha 2-Antiplasmin depleted plasma clots were more susceptible to lysis by both endogenous and exogenous t-PA than normal clots.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Zamarron C,Lijnen HR,Collen Ddoi
10.1016/0049-3848(84)90364-5subject
Has Abstractpub_date
1984-08-01 00:00:00pages
335-45issue
3eissn
0049-3848issn
1879-2472pii
0049-3848(84)90364-5journal_volume
35pub_type
杂志文章abstract::The prevention of venous thromboembolism (VTE) in women with cancer requires special consideration. The presence of cancer results in both non-surgical and surgical patients having increased risk of VTE. This usually leads to a modification of the recommended preventive therapy, affecting the type, dose and length of ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/S0049-3848(11)70003-2
更新日期:2011-02-01 00:00:00
abstract::The effects of extracellular sodium on platelet aggregation and calcium mobilization in platelets stimulated with either rattlesnake (Crotalus atrox) lectin (RSL) or alpha-thrombin were compared. The absence of extracellular sodium had no effect on platelet aggregation or calcium mobilization in response to all levels...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90008-k
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND:Integrin αIIbβ3 mediates platelet adhesion, aggregation and fibrin clot retraction. These processes require activation of αIIbβ3 and post-ligation signaling. Disulfide bond exchanges are involved in αIIbβ3 and αvβ3 activation. METHODS:In order to investigate the role of integrin activation and disulfide bon...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.02.001
更新日期:2014-05-01 00:00:00
abstract::von Willebrand Factor (vWF) is released from endothelial cells. Increased vWF in the coronary circulation during cardiac surgery could be a potential indicator of coronary endothelial injury or stimulation, and thus a possible tool to evaluate regimens of myocardial protection. Release of vWF was investigated in 12 pa...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90050-7
更新日期:1994-01-01 00:00:00
abstract::Recombinant factor IX (rFIX) is increasingly used to treat patients with hemophilia B. CHO (Chinese Hamster Ovary) cells are commonly used for the production of rFIX but they have a limited capacity for introducing post-translational modifications (PTM) leading to incomplete γ-carboxylation, low phosphorylation and su...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.08.313
更新日期:2012-11-01 00:00:00
abstract::Aim of this study was to evaluate rapid D-Dimer tests for their utility in diagnosis of acute pulmonary embolism (PE). Tests were performed in 183 consecutive pats referred for lung scanning because of clinically suspected PE. According to lung scans and the clinical course of disease 19 pats were classified to have P...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00089-8
更新日期:1996-06-01 00:00:00
abstract::The perfusion technique to measure the relative adhesive and thrombogenic indices of vascular prostheses by a quantitative scanning electron microscopy has been described. To normalize the experiments with different samples of plasma we compared the adhesive and thrombogenic properties of the analyzed prostheses with ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90038-5
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Pregnancy is a hypercoagulable state associated with an increased risk of venous thrombosis, which begins during the first trimester, but the exact time of onset is unknown. Thrombin generation, a laboratory marker of thrombosis risk, increases during normal pregnancy but it is unclear exactly how early this...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.06.027
更新日期:2017-09-01 00:00:00
abstract::Human apolipoprotein B was purified by barium sulphate adsorption, subsequent delipidation and gel filtration. The protein was then reconstituted in soya bean lecithin and its inhibitory effect towards thromboplastin was assayed by incubation with rabbit brain thromboplastin. The use of an antibody against human apoli...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(92)90033-7
更新日期:1992-10-15 00:00:00
abstract::The immunoglobulin superfamily member EMMPRIN (CD147) plays an important role in a number of organ systems, including the cardiovascular system. Here we review the contemporary understanding of EMMPRIN and EMMPRIN-associated sequelae in the course of atherosclerosis. A significant body of data documents the pivotal ro...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2013.04.017
更新日期:2013-06-01 00:00:00
abstract:INTRODUCTION:Post-thrombotic syndrome (PTS) is a chronic sequel of deep vein thrombosis (DVT). The clot structure and fibrinolytic potential in PTS is currently unknown. OBJECTIVE:To assess the fibrinolytic potential and clot structure in patients with PTS. MATERIALS AND METHODS:Patients with a history of DVT were in...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.11.013
更新日期:2016-01-01 00:00:00
abstract::Using high-resolution real-time two-dimensional ultrasound, we have investigated the role of red cells in the growth of already established platelet aggregates under controlled flow conditions. Platelet rich plasma (PRP) was circulated in vitro in horizontally and vertically arranged tubing at mean shear rate ranging ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90376-1
更新日期:1984-10-01 00:00:00
abstract::Fasting plasma homocysteine level and the related clinical findings were analysed in 240 consecutive patients with venous thromboembolism. Hyperhomocysteinemia, defined as a plasma level above 20 micromol/l (corresponding to the percentile 95th in the controls), was present in 11.2% of the patients. Plasma homocystein...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00096-8
更新日期:2002-04-15 00:00:00
abstract::The extrinsic pathway of coagulation is initiated when zymogen factor VII binds to its cell surface receptor tissue factor. Recently recombinant human tissue factor has become available and therefore in this study the direct binding of human factor VII in plasma to recombinant tissue factor was explored. Factor VII bi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90103-u
更新日期:1993-05-15 00:00:00
abstract::Human gel-filtered platelets or platelet-rich plasma were stimulated by sodium arachidonate or by ADP in the presence of two compounds known to inhibit ADP mediated aggregation and secretion - ATP and N-ethylmaleimide. Using gel-filtered platelets and the lowest concentration of agonist necessary to elicit maximum agg...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90105-6
更新日期:1983-09-01 00:00:00
abstract::Blood platelets have recently been shown to express PYK2, a nonreceptor tyrosine kinase belonging to the FAK gene family. In this study, we examined the involvement of protein kinase C (PKC) in PYK2-related responses in human platelets. While PYK2 tyrosine phosphorylation induced by thrombin was inhibited by preincuba...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00188-1
更新日期:1999-03-15 00:00:00
abstract::Capsaicin was found to be a potent inhibitor of platelet aggregation and release reaction. It inhibited the aggregation of rat platelet induced by collagen and thrombin, but only slightly reduced those of AA and A23187. The IC50 on collagen-induced platelet aggregation was about 85 micrograms/ml. Less inhibition was o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90189-0
更新日期:1984-12-15 00:00:00
abstract::Hypercoagulable states can be detected by measuring activation peptides, enzyme-inhibitor complexes, and fibrin/fibrinogen degradation products, which are markers of hemostatic activation. A series of these prethrombotic markers has been evaluated in the elderly, pregnancy, diabetes and acute myocardial infarction pat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00165-0
更新日期:1999-01-15 00:00:00
abstract::We have developed a new bioassay for endothelium-derived relaxing factor (EDRF) or nitric oxide (NO) using human [3H]guanosine triphosphate (GTP)-labeled platelets. The labeled platelets were preincubated with isobutyl-methylxanthine and co-cultured with endothelial cells and the [3H]cyclic guanosine monophosphate (cG...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)00082-3
更新日期:1995-06-15 00:00:00
abstract:INTRODUCTION:In-vitro and in-vivo models suggest the influence of low-molecular weight heparin on conception in infertile women undergoing in vitro fertilization procedures (IVF). In this randomized controlled trial we assessed whether a low-molecular weight heparin (parnaparin) could affect IVF outcomes. MATERIALS AN...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2017.08.006
更新日期:2017-11-01 00:00:00
abstract::It is known that a low-molecular-weight heparin (LMWH) is more effective than unfractionated heparin in unstable angina/non-Q-wave myocardial infarction (UA/NQMI) and the platelet GPIIb/IIIa receptors play an important role in acute myocardial infarction (AMI). Therefore, enoxaparin might have a similar advantage over...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00242-0
更新日期:2001-05-01 00:00:00
abstract::Chloroquine inhibited aggregation of rat blood platelets in a dose-dependent way. The inhibitory effect decreased, depending on the aggregation stimulus used, in the rank order of potency: ADP > thrombin > A23187. Effect of 1 millimolar chloroquine on A23187-stimulated aggregation was significantly decreased or comple...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90270-4
更新日期:1994-06-01 00:00:00
abstract::The sequel of survivors after hospitalisation for an acute myocardial infarction was studied during the following 15 years in 48 patients treated with streptokinase and in 41 controls. No significant difference was seen in the morbidity or mortality during the first 6 months nor in the mortality thereafter. ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90233-0
更新日期:1984-08-15 00:00:00
abstract::Human saphenous veins were analyzed for presence of plasminogen activators (t-PA) in the endothelium of the vessel walls by immunohistochemical techniques. We used a polyclonal rabbit antihuman t-PA antibody, a monoclonal mouse-antihuman t-PA and a goat antibody to low molecular weight urokinase. To demonstrate presen...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90240-9
更新日期:1986-02-01 00:00:00
abstract::Biological effects of the modification of the sulphate ester and carboxyl group content of poly(vinyl alcohol-acrylic acid) copolymers (PAVAS) and sulphated polyvinyl alcohol copolymers (PVAS) with mol. weight of 5,000 to 20,000 D were studied. The in vitro anticoagulant potency of PAVAS assessed by activated partial ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90154-6
更新日期:1994-05-15 00:00:00
abstract::D-dimer of human fibrin was used as antigen to obtain monoclonal antibodies (mAbs). We have obtained 16 hybridomas producing mAbs of different specificity. Only two of these mAbs inhibited fibrin polymerization. They are of the IgG-class. One mAb (II-4d) inhibited fibrin polymerization to 100% and another (II-3b) to 6...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.03.005
更新日期:2004-01-01 00:00:00
abstract::Severe prekallikrein (Fletcher factor) deficiency was diagnosed in a 49-year-old woman and in 3 of her siblings. Functional prekallikrein (PKK) activity was found below 1% by clotting assay and 20% by amidolytic assay in all the affected subjects; PKK cross-reacting material (CRM) was present in all the patients (anti...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90222-x
更新日期:1990-12-01 00:00:00
abstract::Tissue factor is an ubiquitous membrane-anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factors VII and VIIa. Under normal circumstances, endothelial cells do not express tissue factor but, in some pathological situations, when the endothelium or the monocytes are exposed t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90086-8
更新日期:1994-01-15 00:00:00
abstract:BACKGROUND:Plasminogen activator inhibitor type-1 (PAI-1) is considered to be the main inhibitor of fibrinolysis in sepsis. However, the contribution of TAFI to the inhibition of fibrinolysis in sepsis is currently unknown. METHODS:TAFI antigen and PAI-1 levels were measured in severe sepsis (n = 32) and septic shock ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.06.007
更新日期:2006-01-01 00:00:00
abstract::When activated in vitro, thrombin-activatable fibrinolysis inhibitor (TAFI) slows clot lysis by cleaving the C-terminal lysine and arginine residues from partially degraded fibrin. An inhibitor of carboxypeptidase isolated from potato (CPI) reverses prolongation of clot lysis by inhibiting activated TAFI. We investiga...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00184-5
更新日期:2000-05-15 00:00:00