Abstract：
:The vector potential of Culicoides variipennis for Rift Valley fever virus (RVFV) was investigated. Insects from a colony maintained at the Animal Virus Research Institute, Pirbright, were fed through a membrane on a mixture of mouse blood and RVFV (virus concentration of blood meal 7 X 95 log10 MLD50ml). Engorged insects were maintained at 25 degrees C +/- 1 degrees C. Samples of insects were tested at daily intervals to determine their virus content. Four of the five females tested immediately after feeding contained virus. The mean virus concentration of these infected flies was 2 X 7 log10MLD50. The virus concentration per fly decreased to 0 by day 2 post infection. On day 3, a virus concentration of 2 X 4 log10 MLD50 per fly was recorded from a pool of 17 flies but between day 4 and day 12 when the experiment terminated no virus was detected in any of the 135 females tested. Because of the pathogenic nature of RVFV, this work was carried out under the stringent security regulations at the PHLS Centre for Applied Microbiology and Research, Porton Down. The problems arising from experiments requiring the handling and infection of insects under such conditions are described.
journal_name
Trans R Soc Trop Med Hygauthors
Jennings M,Platt GS,Bowen ETdoi
10.1016/0035-9203(82)90216-4subject
Has Abstractpub_date
1982-01-01 00:00:00pages
587-9issue
5eissn
0035-9203issn
1878-3503journal_volume
76pub_type
杂志文章abstract：:Kidneys were studied by light microscopy in 246 consecutive autopsies in Cairo, Egypt. Glomerulonephritis was not related to the presence or intensity of either S. haematobium or S. mansoni infection, and mesangial thickening and proliferation were also unrelated to schistosome infection. Acute and chronic pyelonephri...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(76)90088-2
更新日期：1976-01-01 00:00:00
abstract：:Indirect enzyme-linked immunosorbent assay (ELISA) allows sensitive detection of serum immunoglobulin (Ig) G against a soluble extract of Strongyloides stercoralis infective larvae. In this study, 40/40 (100%) human strongyloidiasis sera had high levels of anti-S. stercoralis IgG, but 30/40 (75%) filariasis sera, and ...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(93)90477-8
更新日期：1993-03-01 00:00:00
abstract：:Malaria remains an important health problem in Bangladesh, with approximately 14 million people at risk. Antimalarial drug resistance is a major obstacle to the control of malaria in endemic countries. In 2012, Bangladesh reported an estimated 29 522 malaria episodes, of which 94% were reported as being caused by Plas...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章,评审
doi：10.1093/trstmh/trt088
更新日期：2013-12-01 00:00:00
abstract：:In order to understand better the relationship among Mycobacterium leprae, its transmission and the human host or the chain of infection which may lead to the development of leprosy, we performed a population survey in which nasal carriage of M. leprae was determined by a specific polymerase chain reaction (PCR), 2 ye...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(95)90018-7
更新日期：1995-07-01 00:00:00
abstract：:A study was conducted in rural Malawi to verify (a) whether the Partec CyFlow Counter((R)) for CD4+ T-cell lymphocyte counting in HIV-positive individuals could be introduced into a district hospital laboratory and (b) whether it would produce CD4 counts of acceptable quality. CD4+ cell counting was performed using th...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章,多中心研究
doi：10.1016/j.trstmh.2005.11.014
更新日期：2006-10-01 00:00:00
abstract：:Although intestinal helminth and protozoan infections are prevalent throughout the world, their impact is poorly known. Nevertheless, the morbidity and mortality that occur have stimulated research into host resistance and pathogenesis. Unfortunately, despite an increasing knowledge base, the actual effector molecules...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章,评审
doi：10.1016/0035-9203(86)90372-x
更新日期：1986-01-01 00:00:00
abstract：:A survey for Schistosoma intercalatum conducted in Kinshasa, Zaire, in September 1994 revealed a prevalence of 3.6% (n = 167). Three isolates of schistosomes were made by exposing Bulinus wrighti to miracidia hatched from eggs collected from 2 infected children. Characterization of the isolates by biochemical (isoenzy...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(97)90068-7
更新日期：1997-05-01 00:00:00
abstract：:A 29-year-old Dutch tourist of the Caucasian race had to break her return journey from Indonesia as she developed dengue haemorrhagic fever. She appeared to have been infected on Bali. Serological investigation revealed very high antibody titres against dengue virus types 1 and 3 and a moderately high titre against ty...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(83)90068-8
更新日期：1983-01-01 00:00:00
abstract：:Rickettsia tsutsugamushi isolations were attempted from blood samples obtained from rats captured in four adjacent habitats near Kuala Lumpur, Malaysia. Antibody surveys were also made. Rickettsial infections were most frequent in rats captured in the forest and in lalang grass (Imperata cylindrica) and least frequent...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(77)90142-0
更新日期：1977-01-01 00:00:00
abstract：:261 individuals were skin tested with leishmanin from Leishmania donovani and with an antigen from a lizard trypanosome isolated in an area endemic for kala-azar in Ethiopia. 83 of the 261 responded positively, with indurations of 5 mm or greater, to one antigen but negatively to the other, and 42 reacted positively t...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(80)90247-3
更新日期：1980-01-01 00:00:00
abstract：:Diarrhoeal diseases are a major cause of malnutrition, partly due to poor dietary practices. Misconceptions among the general population and medical personnel lead to withholding of food or avoiding the use of nutritious, locally available and affordable foods. Breast-feeding should not be interrupted during diarrhoea...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(91)90138-o
更新日期：1991-01-01 00:00:00
abstract：:The commercial dot-blot enzyme-linked immunosorbent assay Dip-S-Ticks dipstick test was compared with the indirect immunoperoxidase (IIP) and Weil-Felix (WF) tests for the diagnosis of scrub typhus, using the indirect immunofluorescent antibody test (IFA) as the reference standard. With a panel of 117 positive and 75 ...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(97)90266-2
更新日期：1997-07-01 00:00:00
abstract：:Antibody to Ebola virus was found in 14 (1.2%) of 1147 human sera collected in Gabon in 1981-1997. Six seropositive subjects were bled in the northeast in 1991, more than 3 years prior to recognition of the first known outbreak of Ebola haemorrhagic fever (EHF), whilst eight came from the southwest where the disease h...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/j.trstmh.2006.07.002
更新日期：2007-01-01 00:00:00
abstract：:Between May and September 1982 an extensive epidemic of poliomyelitis occurred in Gazankulu in the north-eastern region of South Africa, with a total of 260 paralysed cases and 42 deaths. A three-part study was instituted, the first of which established the aetiological agent of the epidemic to be poliovirus type 1. T...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(84)90166-4
更新日期：1984-01-01 00:00:00
abstract：:The relation between Plasmodium falciparum malaria and symptomatic human immunodeficiency virus 1 (HIV-1) infection was investigated in paediatric and adult patients in Kampala, Uganda, from 1987 to 1989. Both infections contributed largely to hospital morbidity. Of 1527 clinically suspicious in-patients, 61% were pos...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(90)90306-y
更新日期：1990-05-01 00:00:00
abstract：BACKGROUND:The objective of study was to estimate the incremental cost-effectiveness ratio (ICER) of diagnostic options for visceral leishmaniasis (VL) in Brazil. METHODS:Six diagnostic tests were considered: IT LEISH, Kalazar Detect, DAT-LPC (DAT made in the Laboratório de Pesquisas Clínicas), IFAT, PCR and direct ex...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1093/trstmh/trw050
更新日期：2016-08-01 00:00:00
abstract：:The extent to which ivermectin treatments have an impact on onchocerciasis transmission is a matter of some concern. We investigated this issue in the Mbam valley, a hyperendemic focus located in a forest-savannah mosaic area of Cameroon. Parasitological examinations of 5-9-year-old children, who had never received an...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/j.trstmh.2003.11.010
更新日期：2004-09-01 00:00:00
abstract：:Isoenzyme profiles of 28 stocks of Leishmania aethiopica were compared with those of reference strains of L. aethiopica, L. tropica and L. major using starch-gel electrophoresis of 13 enzymes (GPI, GD, ES, PGM, PEPD, NH, ASAT, ALAT, PK, MPI, 6PGD, SOD, MDH). 13 zymodemes were seen. L. aethiopica showed some infraspeci...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(86)90318-4
更新日期：1986-01-01 00:00:00
abstract：:In 25 cases of pellagra and 10 healthy controls, the blood pyruvic acid levels in the fasting stage and after 60 and 90 minutes of glucose load were estimated by the technique of Friedmann & Haugen (1943). The blood pyruvic acid levels after 60 and 90 minutes of glucose load were significantly higher in pellagrins as ...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(77)90200-0
更新日期：1977-01-01 00:00:00
abstract：:Prolonged carriage of Plasmodium falciparum in humans during the dry season is critical for parasite survival, as the infected subjects constitute a major reservoir in the absence of transmission. Yet, very little is known about the host/parasite interactions contributing to parasite persistence. In order to study the...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(99)90122-0
更新日期：1999-07-01 00:00:00
abstract：:Malaysian, British and New Zealand soldiers were tested for evidence of infection with Rickettsia tsutsugamushi after several weeks' exposure to the infection during field exercises in Malaysia. 39 (5.0%) of 787 British and New Zealand soldiers developed immunofluorescent antibody (IFA) to R. tsutsugamushi to a titre ...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(83)90079-2
更新日期：1983-01-01 00:00:00
abstract：:In order to help primary health care (PHC) workers in developing countries in the care of common skin diseases, an algorithm for the management of pyoderma, scabies, superficial mycoses, contact dermatitis and referral of early leprosy cases (based on the identification of diseases through the presence of objective ke...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/j.trstmh.2004.03.008
更新日期：2005-01-01 00:00:00
abstract：:From the early 1900s, visceral leishmaniasis (VL; kala-azar) has been among the most important health problems in Sudan, particularly in the main endemic area in the eastern and central regions. Several major epidemics have occurred, the most recent--in Western Upper Nile province in southern Sudan, detected in 1988--...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(01)90218-4
更新日期：2001-04-01 00:00:00
abstract：:Guinea-pigs were inoculated with Entamoeba histolytica strains isolated from cases of amoebic liver abscess, amoebic dysentery and asymptomatic cyst passers and the strains were classified in zymodemes by isoenzyme electrophoresis. It was observed that certain non-pathogenic zymodemes were potentially pathogenic for t...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(89)90385-4
更新日期：1989-09-01 00:00:00
abstract：:Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose and medium supplemented with 5 or 10% foetal calf serum at 27 degrees C on a rotating shaker platform. 85 to 95% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effe...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(82)90169-9
更新日期：1982-01-01 00:00:00
abstract：:The human immunodeficiency virus (HIV) epidemic is associated with a marked increase of tuberculosis cases. The influence of HIV on diagnostic methods for tuberculous lymphadenitis is less clear. In an environment of high HIV and tuberculosis prevalence in Blantyre, Malawi, a prospective study compared results of basi...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(97)90081-x
更新日期：1997-05-01 00:00:00
abstract：:A survey of Crimean-Congo haemorrhagic fever virus antibody, using the reversed passive haemagglutination inhibition test, was carried out in Saudi Arabia on sera from imported livestock at Jeddah seaport, as well as humans who had contact with imported animals on farms and in quarantine stations. Antibodies were dete...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/s0035-9203(97)90014-6
更新日期：1997-09-01 00:00:00
abstract：:In highly malaria-endemic areas, thick smears are usually positive regardless of the clinical context. Therefore the simple positivity or negativity of the thick smear is not an adequate criterion for distinguishing malaria from other causes of fever. In order to define simple parasitological and clinical criteria for...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(85)90054-9
更新日期：1985-01-01 00:00:00
abstract：:Cutaneous leishmaniasis (CL) is endemic in Algeria where two forms have been previously described, the sporadic form caused by Leishmania infantum in the north and the cutaneous form caused by L. major in central and southern parts of the country. During 2005, a CL outbreak occurred in the province of Ghardaïa, locate...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/j.trstmh.2009.04.013
更新日期：2009-07-01 00:00:00
abstract：:Anthropometric measurements were made and serum iron and ferritin levels determined in a group of Gambian children at the beginning of the rainy season and these findings were related to the malaria experience of the children during the following malaria transmission season. Susceptibility to malaria was not correlate...
journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type： 杂志文章
doi：10.1016/0035-9203(91)90351-x
更新日期：1991-09-01 00:00:00
