Serum IL-10 involved in morphine tolerance development during adjuvant-induced arthritis.


:Opioid receptors play an important role in modulation of hyperalgesia in inflamed tissues, but chronic morphine application induces such side effects as tolerance. There is near communications between cytokines and mu opioid receptor expression. This study was aimed to assess the role of serum IL-10 in morphine tolerance development during adjuvant-induced arthritis. Adjuvant arthritis (AA) was induced on day 0 by single injection of Complete Freund's Adjuvant (CFA) into the rats' hindpaw. Hyperalgesia, edema, and spinal mu opioid receptor (mOR) variations were assessed on 0, 7, 14, and 21 days of the study. For assessment of the morphine tolerance development, morphine effective dose (4 mg/kg) was administered from the 14th day after CFA injection and continued until the morphine post-dose paw withdrawal latency (PWL); it did not significantly differ from the baseline. For assessment of the effects of IL-10 on tolerance induction, a neutralizing dose (ND50) of anti-IL-10 was administered daily during different stages of the study. AA induction in the right hindpaw of rats resulted in unilateral inflammation and hyperalgesia within 21 days of the study. Anti-IL-10 antibody administration in the AA rats induced marked elevation of hyperalgesia compared to the AA control group. Our data also indicated that morphine effective anti-hyperalgesic dose significantly decreased in the AA rats compared to the control group, which this symptom was aligned with spinal mu opioid receptor (mOR) expression increase during AA. Moreover, there was a significant difference in morphine tolerance induction between the AA and control rats, and our results also demonstrated that IL-10 played an important role in tolerance-induction process. It can be concluded that morphine tolerance slowly progressed when administered morphine effective dose was reduced during AA chronic inflammation. On the other hand, it seems that increased level of serum IL-10 may affect morphine tolerance development during inflammation.


J Physiol Biochem


Zaringhalam J,Hormozi A,Tekieh E,Razavi J,Khanmohammad R,Golabi S




Has Abstract


2014-06-01 00:00:00












  • Catalase inhibition in diabetic rats potentiates DNA damage and apoptotic cell death setting the stage for cardiomyopathy.

    abstract::Diabetes is a risk factor for cardiovascular disease that has a multifactorial etiology, with oxidative stress as an important component. Our previous observation of a significant diabetes-related increase in rat cardiac catalase (CAT) activity suggested that CAT could play a major role in delaying the development of ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Ivanović-Matić S,Bogojević D,Martinović V,Petrović A,Jovanović-Stojanov S,Poznanović G,Grigorov I

    更新日期:2014-12-01 00:00:00

  • Role of omega-3 fatty acids in obesity, metabolic syndrome, and cardiovascular diseases: a review of the evidence.

    abstract::The present review aims to illustrate current knowledge about the efficacy of omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs) in treating/preventing several metabolic pathologies. We reviewed systematically the published evidence on the effectiveness of n-3 LC-PUFAs fish consumption or n-3 LC-PUFAs suppl...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审


    authors: Lorente-Cebrián S,Costa AG,Navas-Carretero S,Zabala M,Martínez JA,Moreno-Aliaga MJ

    更新日期:2013-09-01 00:00:00

  • About the controversies of the cardioprotective effect of n-3 polyunsaturated fatty acids (PUFAs) between animal studies and clinical meta-analyses: a review with several strategies to enhance the beneficial effects of n-3 PUFAs.

    abstract::Several meta-analyses describing the effect of n-3 polyunsaturated fatty acids on the survival rate of the victims of an acute coronary event do not clearly support a beneficial impact of these fatty acids. Yet, animal studies consistently show n-3 PUFA-induced protection against ischemia-reperfusion-induced myocardia...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审


    authors: Demaison L,Leger T,Vergely C,Rochette L,Azarnoush K

    更新日期:2019-08-01 00:00:00

  • Hypothermia can reverse hepatic oxidative stress damage induced by hypoxia in rats.

    abstract::Our previous findings demonstrated that hypothermia enhances the reduction potential in the liver and helps to maintain the plasmatic antioxidant pool. Here, we aimed to elucidate if hypothermia protects against hypoxia-induced oxidative stress damage in rat liver. Several hepatic markers of oxidative stress were comp...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Garnacho-Castaño MV,Alva N,Sánchez-Nuño S,Bardallo RG,Palomeque J,Carbonell T

    更新日期:2016-12-01 00:00:00

  • Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis.

    abstract::Adipose tissue has long been considered to be involved in tumor progression. However, the adipocyte-secreted molecular determinants that regulate hepatocellular cancer progression have not been defined yet. In this study, the expression pattern of exosome miRNAs in hepatocellular carcinoma (HCC) patients with high bod...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Liu Y,Tan J,Ou S,Chen J,Chen L

    更新日期:2019-08-01 00:00:00

  • Low shear stress damages endothelial function through STAT1 in endothelial cells (ECs).

    abstract::Low shear stress (LSS) occurs in areas where atherosclerosis is prevalent. Many studies have revealed that signal transducer and activator of transcription 1 (STAT1) plays a significant role in cardiovascular disease. Nonetheless, the mechanism underlying the connection between STAT1 and LSS is not fully understood. T...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Zhu L,Wang F,Yang H,Zhang J,Chen S

    更新日期:2020-02-01 00:00:00

  • Age-related regulation of excitation-contraction coupling in rat heart.

    abstract::Hearts from subjects with different ages have different Ca(2+) signaling. Release of Ca(2+) from intracellular stores in response to an action potential initiates cardiac contraction. Both depolarization-stimulated and spontaneous Ca(2+) releases, Ca(2+) transients and Ca(2+) sparks, demonstrate the main events of exc...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Kandilci HB,Tuncay E,Zeydanli EN,Sozmen NN,Turan B

    更新日期:2011-09-01 00:00:00

  • The protective microRNA-199a-5p-mediated unfolded protein response in hypoxic cardiomyocytes is regulated by STAT3 pathway.

    abstract::The protective effects of downregulated miR-199a-5p on ischemic and hypoxic cardiomyocytes were well recognized, but the underlying mechanism of inhibited miR-199a-5p is not yet clear. The present study explored the relationship between enhanced signal transducer and activator of transcription 3 (STAT3) signaling and ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Zhou Y,Pang B,Xiao Y,Zhou S,He B,Zhang F,Liu W,Peng H,Li P

    更新日期:2019-02-01 00:00:00

  • Production of healthier goat milk. Use of a concentrate supplemented with a "protected" fat rich in PUFA.

    abstract::In order to obtain healthier goat milk as far as its fat composition is concerned, milk production and composition trials were carried out with Granadina goats kept in semi-extensive breeding conditions. The animals were feeding indoors with a concentrate supplemented or not with 7% of fat conveniently protected again...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Sanz Sampelayo MR,Martín Alonso JJ,Morón D,Pérez L,Boza J

    更新日期:2000-09-01 00:00:00

  • Effects of overexpression of growth hormone on T cell activity in transgenic mice.

    abstract::Growth hormone plays a key role in the maturation and maintenance of the immune response, however, the effects of chronic high circulating concentrations of the hormone on the immune system is poorly understood. Transgenic mice overexpressing bovine growth hormone (b-GH) gene, fused to the rat phosphoenolpyruvate carb...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Esquifino AI,Arce A,Alvarez MP,Szary A,Brown-Borg H,Bartke A

    更新日期:2002-09-01 00:00:00

  • Effects of reactive oxygen species and interplay of antioxidants during physical exercise in skeletal muscles.

    abstract::A large number of researches have led to a substantial growth of knowledge about exercise and oxidative stress. Initial investigations reported that physical exercise generates free radical-mediated damages to cells; however, in recent years, studies have shown that regular exercise can upregulate endogenous antioxida...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审


    authors: Thirupathi A,Pinho RA

    更新日期:2018-08-01 00:00:00

  • Gene methylation parallelisms between peripheral blood cells and oral mucosa samples in relation to overweight.

    abstract::Epigenetics has an important role in the regulation of metabolic adaptation to environmental modifications. In this sense, the determination of epigenetic changes in non-invasive samples during the development of metabolic diseases could play an important role in the procedures in primary healthcare practice. To help ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: San-Cristobal R,Navas-Carretero S,Milagro FI,Riezu-Boj JI,Guruceaga E,Celis-Morales C,Livingstone KM,Brennan L,Lovegrove JA,Daniel H,Saris WH,Traczyk I,Manios Y,Gibney ER,Gibney MJ,Mathers JC,Martinez JA

    更新日期:2016-08-01 00:00:00

  • Pontine A5 region modulation of the cardiorespiratory response evoked from the midbrain dorsolateral periaqueductal grey.

    abstract::Connections between the midbrain dorsolateral periaqueductal grey (dlPAG) and the pontine A5 region have been shown. The stimulation of both regions evokes similar cardiovascular responses: tachycardia and hypertension. Accordingly, we have studied the interactions between dlPAG and A5 region in spontaneously breathin...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: López-González MV,González-García M,Peinado-Aragonés CA,Barbancho MÁ,Díaz-Casares A,Dawid-Milner MS

    更新日期:2020-11-01 00:00:00

  • Lipoic acid prevents body weight gain induced by a high fat diet in rats: effects on intestinal sugar transport.

    abstract::Several studies have suggested that oxidative stress might cause and aggravate the inflammatory state associated with obesity and could be the link between excessive weight gain and its related disorders such as insulin resistance and cardiovascular diseases. Thus, antioxidant treatment has been proposed as a therapy ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Prieto-Hontoria PL,Pérez-Matute P,Fernández-Galilea M,Barber A,Martínez JA,Moreno-Aliaga MJ

    更新日期:2009-03-01 00:00:00

  • AICAR inhibits oxygen consumption by intact skeletal muscle cells in culture.

    abstract::Activation of 5' adenosine monophosphate-activated protein kinase (AMPK) with aminoimidazole carboxamide ribonucleotide (AICAR) increases skeletal muscle glucose uptake and fatty acid oxidation. The purpose of these experiments was to utilize AICAR to enhance palmitate consumption by mitochondria in cultured skeletal ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Spangenburg EE,Jackson KC,Schuh RA

    更新日期:2013-12-01 00:00:00

  • Melatonin modulates Ca2+ mobilization and amylase release in response to cholecystokinin octapeptide in mouse pancreatic acinar cells.

    abstract::In the present work, we have evaluated the effect of an acute addition of melatonin on cholecystokinin octapeptide (CCK-8)-evoked Ca(2+) signals and amylase secretion in mouse pancreatic acinar cells. For this purpose, freshly isolated mouse pancreatic acinar cells were loaded with fura-2 to study intracellular free C...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Santofimia-Castaño P,Ruy DC,Salido GM,González A

    更新日期:2013-12-01 00:00:00

  • miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.

    abstract::Differentiation of adipocytes and their aggregation to adipose tissue are critical for mammalian growth and development. MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that play important roles in adipogenesis and lipid metabolism. miR-128-3p may contribute to adipose tissue development according t...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Chen C,Deng Y,Hu X,Ren H,Zhu J,Fu S,Xie J,Peng Y

    更新日期:2018-08-01 00:00:00

  • Liver histone H3 methylation and acetylation may associate with type 2 diabetes development.

    abstract::Type 2 diabetes (T2D) is a complicated systemic disease, and the exact pathogenetic molecular mechanism is unclear. Distinct histone modifications regulate gene expression in certain diseases, but little is known about histone epigenetics in diabetes. In the current study, C57BL/6 J mice were used to build T2D model, ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Tu P,Li X,Ma B,Duan H,Zhang Y,Wu R,Ni Z,Jiang P,Wang H,Li M,Zhu J,Li M

    更新日期:2015-03-01 00:00:00

  • Effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an atherogenic diet.

    abstract::Conjugated linoleic acid (CLA) refers to a mixture of naturally occurring positional and geometric isomers of linoleic acid that exist in dairy products and meat. The aim of the present work was to study the effects of c-9,t-11 and t-10,c-12 CLA isomers on body fat accumulation and serum lipids in hamsters fed an athe...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Navarro V,Zabala A,Macarulla MT,Fernández-Quintela A,Rodríguez VM,Simón E,Portillo MP

    更新日期:2003-09-01 00:00:00

  • Effects of different doses of resveratrol on body fat and serum parameters in rats fed a hypercaloric diet.

    abstract::Recently resveratrol, a compound naturally occurring in various plants, has been proposed as a potential anti-obesity compound. The aim of the present work was to analyse the effects of different doses of resveratrol on body fat and serum parameters in rats. Thirty-two male Sprague-Dawley rats were randomly divided in...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Macarulla MT,Alberdi G,Gómez S,Tueros I,Bald C,Rodríguez VM,Martínez JA,Portillo MP

    更新日期:2009-12-01 00:00:00

  • Construction of chimeric cytosolic fructose-1,6-bisphosphatases by insertion of a chloroplastic redox regulatory cluster.

    abstract::In order to transform cytosolic fructose-1,6-bisphosphatases (FBPase)(EC into potential reductively-modulated chloroplast-type enzymes, we have constructed four chimeric FBPases, which display structural viability as deduced by previous modelling. In the X1-type BV1 and HL1 chimera the N-half of cytosolic su...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Cazalis R,Chueca A,Sahrawy M,López-Gorgé J

    更新日期:2004-03-01 00:00:00

  • Multi-regulatory network of ROS: the interconnection of ROS, PGC-1 alpha, and AMPK-SIRT1 during exercise.

    abstract::Transcriptional factors are easily susceptible to any stimuli, including exercise. Exercise can significantly influence PGC-1 α and AMPK-SIRT1 pathway, as it is involved in the regulation of energy metabolism and mitochondrial biogenesis. Exercise is a major energy deprivation process by which many of transcription fa...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审


    authors: Thirupathi A,de Souza CT

    更新日期:2017-11-01 00:00:00

  • Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats.

    abstract::Lipoic acid (LA) is an antioxidant with therapeutic properties on several diseases like diabetes and obesity. Apelin is a novel adipokine with potential beneficial actions on glucose metabolism and insulin resistance. The aim of this study was to examine in 3T3-L1 adipocytes the effects of LA on apelin gene expression...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Fernández-Galilea M,Pérez-Matute P,Prieto-Hontoria P,Martínez JA,Moreno-Aliaga MJ

    更新日期:2011-09-01 00:00:00

  • Double-stranded Let-7 mimics, potential candidates for cancer gene therapy.

    abstract::MicroRNAs (miRNAs), a class of small, single-stranded endogenous RNAs, act as post-transcriptional regulators of gene expression. The ability of one single miRNA regulating multiple functionally related mRNAs makes it a new potential candidate for cancer gene therapy. Let-7s miRNAs have been demonstrated as tumor-supp...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Wang QZ,Lv YH,Gong YH,Li ZF,Xu W,Diao Y,Xu R

    更新日期:2012-03-01 00:00:00

  • Resveratrol pretreatment alleviates myocardial ischemia/reperfusion injury by inhibiting STIM1-mediated intracellular calcium accumulation.

    abstract::Previous studies have shown that stromal interaction molecule1 (STIM1)-mediated store-operated Ca2+ entry (SOCE) contributes to intracellular Ca2+ accumulation in H9C2 cells subjected to hypoxia/reoxygenation(H/R) injury. The aim of the present study was to investigate the effect of resveratrol on STIM1-mediated intra...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Xu H,Cheng J,Wang X,Liu H,Wang S,Wu J,Xu B,Chen A,He F

    更新日期:2019-11-01 00:00:00

  • Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas.

    abstract::The prevalence of non-alcoholic fatty pancreas disease (NAFPD) is increasing in parallel with obesity rates. Stress-related alterations in endoplasmic reticulum (ER), such as the unfolded protein response (UPR), are associated with obesity. The aim of this study was to investigate ER imbalance in the pancreas of a mic...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Soeda J,Mouralidarane A,Cordero P,Li J,Nguyen V,Carter R,Kapur SR,Pombo J,Poston L,Taylor PD,Vinciguerra M,Oben JA

    更新日期:2016-06-01 00:00:00

  • Effects of raloxifene and estradiol on bone turnover parameters in intact and ovariectomized rats.

    abstract::This study was designed to investigate effects of raloxifene (RLX) and estradiol on bone formation and resorption in intact and ovariectomized (ovx) rat models. In the intact model, a total of 24 adult female rats were divided into three groups: Controls subcutaneously received saline alone. RLX (2 mg/kg) and estradio...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Canpolat S,Tug N,Seyran AD,Kumru S,Yilmaz B

    更新日期:2010-03-01 00:00:00

  • Nutritional value of Holothuria forskali protein and effects on serum lipid profile in rats.

    abstract::Two groups of rats were fed on diets with protein from a marine specie holothuria (Holothuria forskali) or casein (for the control group) during 16 days with the aim of assessing the nutritional value of the holothuria protein. Several serum lipid levels were also determined. The results of the nutritive parameters in...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Rodríguez E,González M,Caride B,Lamas MA,Taboada MC

    更新日期:2000-03-01 00:00:00

  • Mechanisms associated to impaired activity of cardiac P-type ATPases in endothelial nitric oxide synthase knockout mice.

    abstract::The effect of long-lasting in vivo restriction of nitric oxide (NO) bioavailability on cardiac and renal P-type ATPases critical for intracellular ion homeostasis is controversial. Previous work has shown in eNOS knockout (eNOS(-/-)) mice hearts that Na(+)/K(+)- and Ca(2+)-ATPase activities were depressed but the unde...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Rezende DC,Pôças ES,Muzi-Filho H,Cunha VM,Caricati-Neto A,Jurkiewicz A,Noël F,Quintas LE

    更新日期:2013-06-01 00:00:00

  • Protein expression changed by nicotine in rat vascular smooth muscle cells.

    abstract::In order to observe the effects of nicotine on protein expression in rat vascular smooth muscle cells (SMCs), nicotine treated SMCs were studied by proteomic technologies combining two-dimensional electrophoresis (2-DE) and peptide mass fingerprinting (PMF). Real-time RT-PCR was used to validate the differentially exp...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章


    authors: Wang Z,Wu W,Fang X,Wang Y,Xiao C,Zhao R,Wang L,Qiao Z

    更新日期:2007-06-01 00:00:00