Evaluation of new agglutination test for identification of oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus.


:A new agglutination test (Monostaph +; Bionor, Skien, Norway) has been developed. This new agglutination test has been compared with two other agglutination tests for the identification of 128 isolates of Staphylococcus aureus and 82 coagulase-negative staphylococci. The sensitivities of both Monostaph + and Pastorex Staph-Plus were excellent (98.7 and 97.4%, respectively) in detection of oxacillin-resistant Staphylococcus aureus. The specificity was 96.4% (two Staphylococcus epidermidis isolates and one Staphylococcus hominis isolate were false positive).


J Clin Microbiol


Tveten Y




Has Abstract


1995-05-01 00:00:00












  • Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period.

    abstract::We have investigated 677 Shiga toxin-producing Escherichia coli (STEC) strains from humans to determine their serotypes, virulence genes, and clinical signs in patients. Six different Shiga toxin types (1, 1c, 2, 2c, 2d, and 2e) were distributed in the STEC strains. Intimin (eae) genes were present in 62.6% of the str...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Beutin L,Krause G,Zimmermann S,Kaulfuss S,Gleier K

    更新日期:2004-03-01 00:00:00

  • Purification of the 115-kilodalton exoantigen of Cryptococcus neoformans and its recognition by immune sera.

    abstract::A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hamilton AJ,Goodley J

    更新日期:1993-02-01 00:00:00

  • Campylobacter species identification based on polymorphism of DNA encoding rRNA.

    abstract::Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsal...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Moureau P,Derclaye I,Gregoire D,Janssen M,Cornelis GR

    更新日期:1989-07-01 00:00:00

  • Panfungal PCR assay for detection of fungal infection in human blood specimens.

    abstract::A novel panfungal PCR assay which detects the small-subunit rRNA gene sequence of the two major fungal organism groups was used to test whole-blood specimens obtained from a series of blood or bone marrow transplant recipients. The 580-bp PCR product was identified after amplification by panfungal primers and hybridiz...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Van Burik JA,Myerson D,Schreckhise RW,Bowden RA

    更新日期:1998-05-01 00:00:00

  • Quantitative measurement of anti-SARS-CoV-2 antibodies: Analytical and clinical evaluation.

    abstract::The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection. While most serologic assays are qualitativ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Higgins V,Fabros A,Kulasingam V

    更新日期:2021-01-22 00:00:00

  • Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping.

    abstract::A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shap...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gubash SM

    更新日期:1978-11-01 00:00:00

  • Detection of bacterial pathogens in purulent clinical specimens by immunofluorescence techniques.

    abstract::A technique is described which may be used to identify Haemophilus influenzae type b, Streptococcus pneumoniae, group A and B streptococci, and Staphylococcus aureus in smears of clinical specimens. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Clausen CR

    更新日期:1981-06-01 00:00:00

  • Comparison of broth microdilution, E Test, and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni and Campylobacter coli.

    abstract::A standardized broth microdilution method was compared to the E test and an agar dilution method for the antimicrobial susceptibility testing of Campylobacter jejuni and C. coli isolates. A group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Luber P,Bartelt E,Genschow E,Wagner J,Hahn H

    更新日期:2003-03-01 00:00:00

  • Canine strangles case reveals a new host susceptible to infection with Streptococcus equi.

    abstract::We report the first documented case of canine strangles due to infection with Streptococcus equi in a dog with enlarged lymph nodes. Genetic typing, via sequencing of 12 housekeeping genes and the SeM gene, demonstrated the isolate to be a member of a common equine strain type circulating in the United Kingdom. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ladlow J,Scase T,Waller A

    更新日期:2006-07-01 00:00:00

  • Rotavirus gastroenteritis and central nervous system (CNS) infection: characterization of the VP7 and VP4 genes of rotavirus strains isolated from paired fecal and cerebrospinal fluid samples from a child with CNS disease.

    abstract::Rotavirus RNA was detected in the cerebrospinal fluid (CSF) of a child with central nervous system disease symptoms associated with rotavirus gastroenteritis. The rotavirus isolates from the fecal and CSF samples were genotyped as G1P[8]. Sequence analysis of the VP7 and VP4 proteins derived from the fecal and CSF sam...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Iturriza-Gómara M,Auchterlonie IA,Zaw W,Molyneaux P,Desselberger U,Gray J

    更新日期:2002-12-01 00:00:00

  • Variation in restriction fragment length polymorphisms among serial isolates from patients with Trichophyton rubrum infection.

    abstract::Molecular genotyping of strains of Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gupta AK,Kohli Y,Summerbell RC

    更新日期:2001-09-01 00:00:00

  • Pasteurella haemolytica-like bacterium from a progressive granuloma of cattle in Brazil.

    abstract::Three strains of a Pasteurella haemolytica-like bacterium were isolated from lesions of a progressive granuloma of cattle in southern Brazil. Their characteristics and their differentiation from P. haemolytica varieties and Actinobacillus lignieresii are described. The name "Pasteurella granulomatis" is proposed for t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ribeiro GA,Carter GR,Frederiksen W,Riet-Correa F

    更新日期:1989-06-01 00:00:00

  • Seroepidemiological study of African horse sickness virus in The Gambia.

    abstract::An indirect enzyme-linked immunosorbent assay was used for the screening of horse sera from The Gambia for antibodies against African horse sickness virus (AHSV). The AHSV antigen used for coating was semipurified according to the method of Manning and Chen (Curr. Microbiol. 4:381, 1980); control mock-infected Vero ce...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Staeuber N,Fye B,Zinsstag J,McCullough KC

    更新日期:1993-08-01 00:00:00

  • Genotypic diversity of mutans streptococci in Brazilian nursery children suggests horizontal transmission.

    abstract::Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

    更新日期:2001-06-01 00:00:00

  • Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7.

    abstract::A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were invest...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Krishnan C,Fitzgerald VA,Dakin SJ,Behme RJ

    更新日期:1987-06-01 00:00:00

  • Ocular infection caused by Psychrobacter immobilis acquired in the hospital.

    abstract::The name Psychrobacter immobilis recently has been proposed for a group of chiefly psychrotrophic, aerobic, gram-negative, nonmotile, oxidase-positive coccobacilli commonly found associated with fish, processed meat, and poultry products. This article reports an ocular infection in a 12-day-old newborn who acquired th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gini GA

    更新日期:1990-02-01 00:00:00

  • Development of an enzyme-linked immunosorbent assay for studying Vibrio cholerae cell surface antigens.

    abstract::An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed ag...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cryz SJ Jr,Fürer E,Germanier R

    更新日期:1982-07-01 00:00:00

  • Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.

    abstract::We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Klena JD,Parker CT,Knibb K,Ibbitt JC,Devane PM,Horn ST,Miller WG,Konkel ME

    更新日期:2004-12-01 00:00:00

  • DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains.

    abstract::The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal puls...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Morrison D,Woodford N,Barrett SP,Sisson P,Cookson BD

    更新日期:1999-04-01 00:00:00

  • Quality control limits for voriconazole disk susceptibility tests on Mueller-Hinton agar with glucose and methylene blue.

    abstract::An international collaborative study was performed in order to propose quality control limits for voriconazole disk diffusion tests on Mueller-Hinton agar with 2% glucose and 0.5 micro g of methylene blue per ml. The supplement may be added to the agar before autoclaving, or Mueller-Hinton agar plates may be flooded w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pfaller MA,Barry A,Bille J,Brown S,Ellis D,Meis JF,Rennie R,Rinaldi M,Rogers T,Traczewski M

    更新日期:2004-04-01 00:00:00

  • Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance.

    abstract::Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

    更新日期:1998-06-01 00:00:00

  • Combined immunoaffinity cDNA-RNA hybridization assay for detection of hepatitis A virus in clinical specimens.

    abstract::To apply cDNA-RNA hybridization methods to the detection of hepatitis A virus (HAV) in clinical materials, we developed a two-step method in which a microtiter-based, solid-phase immunoadsorption procedure incorporating a monoclonal anti-HAV capture antibody was followed by direct blotting of virus eluates to nitrocel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jansen RW,Newbold JE,Lemon SM

    更新日期:1985-12-01 00:00:00

  • Role of specific immunoglobulin E to excretory-secretory antigen in diagnosis and prognosis of hookworm infection.

    abstract::Total immunoglobulin E (IgE) and specific IgE were assayed by a radioimmunosorbent test and a reverse enzyme immunoassay in patients with hookworm infections before and after treatment. A total of 77 subjects (30 patients with hookworm infections and 47 subjects as controls) were studied. Both specific and total IgE l...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ganguly NK,Mahajan RC,Sehgal R,Shetty P,Dilawari JB

    更新日期:1988-04-01 00:00:00

  • Genotyping of methicillin-resistant Staphylococcus aureus by assaying for the presence of variable elements associated with mecA.

    abstract::The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

    更新日期:2002-08-01 00:00:00

  • Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva.

    abstract::A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Collot S,Petit B,Bordessoule D,Alain S,Touati M,Denis F,Ranger-Rogez S

    更新日期:2002-07-01 00:00:00

  • Streamlining HIV testing for HIV preexposure prophylaxis.

    abstract::HIV-testing algorithms for preexposure prophylaxis (PrEP) should be optimized to minimize the risk of drug resistance, the time off PrEP required to evaluate false-positive screening results, and costs and to expedite the start of therapy for those confirmed to be infected. HIV rapid tests (RTs) for anti-HIV antibodie...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Guanira JV,Leigler T,Kallas E,Schechter M,Sharma U,Glidden D,Grant RM,iPrEx Study Team.

    更新日期:2015-01-01 00:00:00

  • International surveillance of bloodstream infections due to Candida species: frequency of occurrence and antifungal susceptibilities of isolates collected in 1997 in the United States, Canada, and South America for the SENTRY Program. The SENTRY Participa

    abstract::An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34 medical centers (22 in the United States, 6 in Canada, and 6 in South America). Eighty percent of the BSIs were nosocomia...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pfaller MA,Jones RN,Doern GV,Sader HS,Hollis RJ,Messer SA

    更新日期:1998-07-01 00:00:00

  • Characterization of Borrelia lusitaniae isolates collected in Tunisia and Morocco.

    abstract::Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Younsi H,Sarih M,Jouda F,Godfroid E,Gern L,Bouattour A,Baranton G,Postic D

    更新日期:2005-04-01 00:00:00

  • Diversity among community isolates of methicillin-resistant Staphylococcus aureus in Australia.

    abstract::Community methicillin-resistant Staphylococcus aureus (CMRSA) strains are being isolated with increasing frequency around the world. In Western Australia CMRSA are endemic in geographically remote communities and have been found to belong to five different contour-clamped homogeneous electric field (CHEF) electrophore...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: O'Brien FG,Lim TT,Chong FN,Coombs GW,Enright MC,Robinson DA,Monk A,Saïd-Salim B,Kreiswirth BN,Grubb WB

    更新日期:2004-07-01 00:00:00

  • Canada's first case of a multidrug-resistant Corynebacterium diphtheriae strain, isolated from a skin abscess.

    abstract::A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

    更新日期:2011-11-01 00:00:00