Abstract：
:Seven compounds having varying affinities for peripheral benzodiazepine (p sites) were evaluated in vitro as inhibitors of arachidonic acid-induced rabbit platelet aggregation and thromboxane B2 (TXB2) synthesis. With the exception of flumazenil, all compounds inhibited both parameters with IC50 values ranging from 0.19 to 3.5 microM, with a significant correlation between the inhibition of aggregation and the synthesis of thromboxane B2. The antiaggregatory effect was stereoselective, and inhibition was increased when cellular penetration was favoured by increasing the volume of the organic solvent. The order of potency these compounds is consistent with an effect at intracellular p sites (Ro5-4864 > PK14067 > PK 11195 > PK 14068 > or = clonazepam > or = diazepam > flumazenil). However, the correlation between the aggregation inhibition and the synthesis of TXB2 suggests that microsomal cyclooxygenase may be the intracellular target of these ligands. We therefore propose that this enzyme possesses a site for benzodiazepine ligands which may share certain characteristics with the peripheral benzodiazepine receptor.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Choppin A,Berry CNdoi
10.1016/0049-3848(95)00062-vsubject
Has Abstractpub_date
1995-05-15 00:00:00pages
293-302issue
4eissn
0049-3848issn
1879-2472pii
004938489500062Vjournal_volume
78pub_type
杂志文章abstract：:Clot lysis induced by streptokinase has been studied and the influences of pH and the concentrations of plasminogen, fibrinogen and buffer composition investigated. Variations in the concentrations of plasminogen strongly effect the SK apparent activity, maxima being obtained at different plasminogen concentrations li...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90071-4
更新日期：1991-12-15 00:00:00
abstract：INTRODUCTION:Electronic consultations (eConsults) are asynchronous, consultative electronic-based communications that are aimed to improve efficient and timely access to specialist advice. Our study aim was to evaluate the use and impact of the Canadian Champlain BASE™ (Building Access to Specialists through eConsultat...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.11.002
更新日期：2017-12-01 00:00:00
abstract：INTRODUCTION:Unfractionated heparin (UFH) potentiates platelet aggregation induced by some agonists. P2Y12 and P2Y1 receptors play a major role in amplifying platelet aggregation. We assessed the ability of cangrelor, a selective P2Y12 antagonist, A2P5P, a selective P2Y1 antagonist, and aspirin to block the potentiatin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.09.004
更新日期：2005-01-01 00:00:00
abstract：:Venous thromboembolism (VTE) affects approximately 1 per 1000 persons annually. Although patients are increasingly treated with direct oral anticoagulants, many patients continue to be anticoagulated with vitamin K antagonists (VKA). The most important adverse events during VKA treatment, bleeding and the risk of recu...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.03.022
更新日期：2019-06-01 00:00:00
abstract：:Plasma membranes of pig platelets were isolated and subjected to solubilization with digitonin. Binding of [3H]ketanserin to the solubilized membrane fraction, optimal at pH 7.5, was time and protein dependent and was greatly reduced by increasing ionic strength of the incubation buffer. Saturation analysis and inhibi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90253-1
更新日期：1985-09-15 00:00:00
abstract：INTRODUCTION:Quercetin is widely distributed in plants and has been reported to have effects of anti-inflammation and anti-thrombosis. In this study, we evaluated the protective effect of quercetin on LPS-induced experimental DIC in rabbits, and tried to clarify its mechanism against DIC. MATERIALS AND METHODS:LPS-ind...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.03.002
更新日期：2013-06-01 00:00:00
abstract：:In the treatment of unstable coronary artery disease, direct thrombin inhibitors have shown no or only limited benefit as compared with heparin, despite theoretical advantages. One explanation may be that the direct thrombin inhibitors to a greater extent than heparin have an inhibiting effect on the generation and ac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00029-8
更新日期：1999-07-15 00:00:00
abstract：:Cilostazol is a newly developed antiplatelet drug that has been widely applied for clinical use. Its antiplatelet action appears to be mainly related to inhibition of intracellular phosphodiesterase activity. Our study was designed to investigate inhibitory effects of cilostazol on the expression of activation-depende...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00172-8
更新日期：1999-02-01 00:00:00
abstract：:Intra-ventricular hemorrhage (IVH) occurs predominantly in very low birth weight premature infants. Survivors of severe IVH frequently experience long-term consequences including major neurological deficits. Advances in neonatal and obstetric care in the last decades, have led to a steady decline in mortality and in t...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/S0049-3848(11)70032-9
更新日期：2011-02-01 00:00:00
abstract：:We have investigated fibrinolytic parameters in 33 patients with peripheral arterial occlusive disease (PAOD) before and 6, 24, and 48 hours after percutaneous transluminal angioplasty (PTA) as well as in 35 gender-matched healthy controls, whose mean age was not significantly different from the mean age of the patien...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00002-x
更新日期：1999-05-15 00:00:00
abstract：IMPORTANCE:Unprovoked venous thromboembolism (VTE) can be the first manifestation of cancer. It is unclear if extensive screening for occult cancer including a comprehensive computed tomography (CT) scan of the abdomen/pelvis is cost-effective in this patient population. OBJECTIVE:To assess the health care related cos...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.01.007
更新日期：2017-03-01 00:00:00
abstract：:Lupus anticoagulants (LA) are IgG or IgM antibodies which prolong phospholipid-dependent coagulation tests. For the detection and quantitation of such antibodies, we have developed an ELISA with cephalin as the coating antigen. The sensitivity of this assay was compared to the activated partial thromboplastin time (AP...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90323-5
更新日期：1990-01-15 00:00:00
abstract：:Although the possible occurrence of systemic fibrinogenolysis has been suggested in patients with metastasising prostatic cancer (MPC), direct evidence is lacking. We report on a patient with MPC whose laboratory data were consistent with hyperfibrinolysis: marked decrease of alpha 2-antiplasmin (AP) level (less than ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90047-e
更新日期：1992-06-15 00:00:00
abstract：:In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90187-f
更新日期：1992-05-01 00:00:00
abstract：:Using a stable cross-linked SAK/plg complex, the effects of alpha 2-plasmin inhibitor on plasminogen activation by SAK were investigated. alpha 2-Plasmin inhibitor inhibited dose-dependently plasminogen activation by the SAK/plg complex. When FCB-2 or EACA was added to the reaction mixture of SAK/plg complex and alpha...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90191-0
更新日期：1994-10-15 00:00:00
abstract：BACKGROUND:The diagnostic work-up for heparin induced thrombocytopenia (HIT) can take several days. Consequently patients may be speculatively switched onto replacement anticoagulant therapy before a diagnosis is confirmed. On-demand immunoassay diagnostic testing enables timely treatment decisions, based on test resul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2016.01.025
更新日期：2016-04-01 00:00:00
abstract：:Human and bovine alpha-thrombins (greater than 90% alpha form) with high fibrinogen clotting activities (approximately 3,000 U.S. units/mg protein) exhibit similar Michaelis menten kinetics with the p-nitroanilide tripeptide substrates Tos-Gly-Pro-arg-pNA (Chromozym-TH) and D-Phe-Pip-Arg-pNA (S-2238). The kinetic para...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90114-1
更新日期：1982-11-01 00:00:00
abstract：INTRODUCTION:Unfractionated heparin (UFH) does not cross the placenta and has demonstrated utility in the prevention and treatment of thrombosis during pregnancy. Limited information is available to guide initiation and monitoring of therapeutic UFH targeting an anti-Xa concentration of 0.3-0.7 u/ml during pregnancy. T...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.07.014
更新日期：2010-05-01 00:00:00
abstract：:Fucoidan [sulfated poly (L-fucopyranose)] was compared with 6-aminohexanoic acid (6-AH) or CNBr-cleaved fibrinogen (CNBr-Fbg) alone or in combination in enhancing the activation of glutamic plasminogen (Glu-Plg) or lysine plasminogen (Lys-Plg) by two-chain tissue plasminogen activator (t-PA) or LMwt-urokinase or by st...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00111-4
更新日期：1995-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:According to US Food and Drugs Administration (FDA), 2 hour recombinant tissue plasminogen activator (rt-PA) 100mg infusion is recommended for eligible patients with acute pulmonary embolism (PE). However,there exists evidence implying that a lower dosage of rt-PA can be equally effective but p...
journal_title：Thrombosis research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.thromres.2013.12.026
更新日期：2014-03-01 00:00:00
abstract：INTRODUCTION:This study determines the impact of tissue factor (TF)-signaling on the extrinsic pathway of apoptosis in cancer cells and propose death associated protein kinase-1 (DAPK1) as a novel key regulator. MATERIALS AND METHODS:In MDA-MB-231 breast and PC3 prostate cancer cells, mRNA levels were analyzed by real...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.11.015
更新日期：2011-02-01 00:00:00
abstract：:Coronary artery disease (CAD) continues to be the most frequent cause of death among women in the United States. Although elevated levels of clotting factors have been associated with CAD, few of these studies have been performed in women. Elevated levels of Factor XI have previously been associated with venous thromb...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00190-1
更新日期：2002-07-15 00:00:00
abstract：INTRODUCTION:Bivalirudin is an alternative to heparin anticoagulation in infants and children in the setting of extracorporeal life support (ECLS). While activated partial thromboplastin time (aPTT) is widely accepted as the standard test to monitor bivalirudin therapy, the usefulness of thromboelastometry (ROTEM) to m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.12.007
更新日期：2020-02-01 00:00:00
abstract：:A rapid method is described for the production of a human marrow cell suspension highly enriched in megakaryocytes. These concentrates were incubated with radiolabelled amino-acids, and cell lysates were then analysed for fibrinogen synthesis. Neosynthesized proteins were detected by immunoprecipitation, immuno-affini...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90133-1
更新日期：1985-05-15 00:00:00
abstract：INTRODUCTION:Excessive platelet activation fundamentally contributes to cardiovascular events and mortality in patients with diabetes mellitus. Functional resistance has been described for current antiplatelet therapies in broad populations that include patients with diabetes. We investigated acute and chronic effects ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.09.025
更新日期：2010-03-01 00:00:00
abstract：INTRODUCTION:Thrombomodulin (TM) has been described as a marker of endothelial injury in atherosclerosis. The role of TM as a predictor of PAD severity is to be proven. The goal of the present study is to compare the level of plasmatic (TMp) in patients with intermittent claudication with patients with critical ischemi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2005.03.010
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:The contact system proteins factor XII (FXII), prekallikrein (PK) and high molecular weight kininogen (HK) have roles in coagulation, fibrinolysis, thrombin-induced platelet activation, cell adhesion and angeogenisis. It has been suggested that inherited or acquired deficiencies of these proteins may be ri...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2004.05.005
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:It has been previously shown that platelets of patients with diabetes are more reactive and less responsive to anti-platelet drugs compared with platelets from subjects without diabetes. Studies examining the effect of glycemic control on platelet reactivity have yielded conflicting data. Thus, in this study...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.05.010
更新日期：2014-07-01 00:00:00
abstract：:G-proteins play a major role in cell signaling and specific abnormalities in these proteins have been implicated in some diseases. Although, two human G alpha q coding sequences are published, they differ at functionally important regions. We have sequenced the human G alpha q cDNA synthesized from normal platelet mRN...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00071-1
更新日期：1998-07-01 00:00:00
abstract：:A direct assay for plasminogen activator (PA) was developed. It employed polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and beta-mercaptoethanol to monitor PA-mediated conversion of single chain, 125I-plasminogen to two chain plasmin. By incorporating Triton X-100, albumin and trasylol in...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90008-2
更新日期：1984-05-01 00:00:00
