Abstract:
:Glutathione is important in cellular defense against oxidative stress. We postulated that administration of N-acetylcysteine (NAC), a glutathione precursor, might help maintain or replenish hepatic glutathione stores, thereby reducing reperfusion injury in liver grafts after warm ischemia. Eighteen pigs were subjected to 2 hr of warm hepatic ischemia and divided into a control group (group A, n = 6), a preischemia treatment group (group B, n = 6: NAC, 150 mg/kg, continuous i.v. infusion 1 hr before ischemia), and a postischemia treatment group (group C, n = 6: NAC, 150 mg/kg continuous i.v., begun 20 min before reperfusion and continued for 1 hr). At initiation of laparotomy, we measured hepatic levels of reduced glutathione (GSH), its oxidized form (GSSG), ATP, aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). Before reperfusion, after 2 hr of warm ischemia, GSH, GSSG, and ATP were measured. One hour after reperfusion, we measured GSH, GSSG, ATP, AST, and LDH. Bile output was recorded every 10 min. Postoperfusion AST and LDH were significantly lower in both treatment groups than in controls. In group B, hepatic glutathione was maintained at significantly higher levels than in controls, even after ischemia (P < 0.05). In group C, although hepatic GSH levels fell until reperfusion, after administration of NAC, hepatic GSH reached the level of the preischemia treatment group. In both treatment groups, GSH 1 hr after reperfusion was significantly higher than in the controls (P < 0.01): regeneration of glutathione was seen in all 6 animals in group C, compared with 2/6 in group B and none in the control group. ATP recovery, bile output, and survival were all better in the treatment groups than in the control group. Pretreatment with NAC helps maintain hepatic glutathione during warm ischemia; given after ischemia, NAC is effective in replenishing depleted glutathione stores. Adjunctive use of NAC was associated with improved glutathione homeostasis, improved bile output and ATP regeneration, and increased survival.
journal_name
Transplantationjournal_title
Transplantationauthors
Fukuzawa K,Emre S,Senyuz O,Acarli K,Schwartz ME,Miller CMdoi
10.1097/00007890-199501150-00002subject
Has Abstractpub_date
1995-01-15 00:00:00pages
6-9issue
1eissn
0041-1337issn
1534-6080journal_volume
59pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::Transplantation provides life-saving therapy to critically ill patients with end-stage organ failure. We found that the specialized knowledge of mental health clinicians and physicians, committed to the evaluation and management of transplant recipients, is essential for the optimal care of these patients who require ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e3181a754d4
更新日期:2009-06-27 00:00:00
abstract:BACKGROUND:Donor-specific immune tolerance has been reported in isolated cases of kidney transplantation associated with bone marrow transplantation. The following is a description of a living donor liver transplantation for a hilar cholangiocarcinoma in a previous recipient of an allogeneic bone marrow transplant. ME...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000083981.82522.13
更新日期:2003-09-27 00:00:00
abstract::Pancreas transplantation utilizing portal venous and enteric exocrine drainage has potential benefits over the standard systemic venous and bladder exocrine drainage method. Unfortunately, technical difficulties are often experienced with the arterial anastomosis after the portal venous anastomosis is completed. We ha...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199907270-00029
更新日期:1999-07-27 00:00:00
abstract::Calcium channel blockade has been shown to prevent warm renal ischemic damage. The ability of verapamil to decrease the severity of acute tubular necrosis (ATN) after 24-hr cold storage and autotransplantation was studied in a randomized paired study of 12 dogs. Experimental animals pretreated with intraarterial verap...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198709000-00006
更新日期:1987-09-01 00:00:00
abstract::The effect of protein depletion on immune responsiveness was examined using a skin allograft model. Protein depletion was induced in adult Fischer (F344) male rats by the ad libitum provision of a 5% protein diet. Total serum protein, total body weight, total body nitrogen, and total body lipid were all markedly decre...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198009000-00012
更新日期:1980-09-01 00:00:00
abstract:BACKGROUND:Urologic complications cause substantial morbidity in the pediatric population after renal transplantation, but their impact on graft survival and transplant costs is poorly understood. In this retrospective review, we evaluated the records of all pediatric renal transplant recipients at our center from 1995...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31818b63da
更新日期:2008-12-15 00:00:00
abstract::T cells must be activated before they can elicit damage to allografts, through interaction of their T cell receptor (TCR) with peptide-MHC complex and through accessory molecules. Signaling through accessory molecules or costimulatory molecules is a critical way for the immune system to fine tune T cell activation. An...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e31826d4672
更新日期:2013-02-27 00:00:00
abstract:BACKGROUND:Kidneys from infectious risk donors (IRD) confer substantial survival benefit in adults, yet the benefit of IRD kidneys to pediatric candidates remains unclear in the context of high waitlist prioritization. METHODS:Using 2010-2016 Scientific Registry of Transplant Recipients data, we studied 2417 pediatric...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000002674
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Whether kidney transplant recipients who were treated for a malignant tumor before transplantation are at an increased risk of developing a tumor posttransplantation has not been adequately quantified and characterized. METHODS:We studied more than 270 000 patients on whom pretransplant and posttransplant m...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002459
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:One of the earliest prerequisites for the development of inflammation after ischemia-reperfusion (I/R) is local chemokine expression. We recently demonstrated that apoptosis, characterized by intracellular caspase-activation, contributes to the development of inflammation after I/R. METHODS:The contribution...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104150-00032
更新日期:2001-04-15 00:00:00
abstract:BACKGROUND:Living-related (LR) human leukocyte antigen (HLA)-identical renal transplant (RTx) recipients often receive standard immunosuppression, despite the absence of mismatched major HLA-antigens and the known complications of long-term use of immunosuppression. No data are available on the need for immunosuppressi...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31819634eb
更新日期:2009-03-15 00:00:00
abstract:BACKGROUND:Postoperative damage of the urethral rhabdosphincter and nerve-vascular networks is a major complication of radical prostatectomy and generally causes incontinence and/or erectile dysfunction. The human skeletal muscle-derived stem cells, which have a synchronized reconstitution capacity of muscle-nerve-bloo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001613
更新日期:2017-10-01 00:00:00
abstract::Noninvasive biomarkers are needed to monitor stable patients following kidney transplantation (KT), as subclinical rejection, currently detectable only with invasive surveillance biopsies, can lead to chronic rejection and graft loss. Several biomarkers have recently been developed to detect rejection in KT recipients...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003064
更新日期:2020-04-01 00:00:00
abstract::Despite major advances in immunopharmacology, virtually all patients receive the same center-specific immunosuppressive regimen following orthotopic liver transplantation (OLT). The present analysis was performed on the hypothesis that the original disease representing the indication for OLT leads to a different initi...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199602270-00007
更新日期:1996-02-27 00:00:00
abstract:BACKGROUND:Blood group-incompatible transplantation is one strategy used when a potential recipient does not have a compatible living donor. Current practice includes desensitization strategies to reduce antibody titers. However, when antibodies are low, in cardiac transplantation in neonates for example, no desensitiz...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825b7608
更新日期:2012-08-27 00:00:00
abstract::In vitro pretreatment of islets of Langerhans with deoxyguanosine (dGuo) has been shown to be effective for the prolongation of islet allograft survival in rats. [This study evaluates the effect of pretreatment of islets with dGuo transplanted into CsA-treated recipients.] Transplantation of dGuo-treated islets from W...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199105000-00007
更新日期:1991-05-01 00:00:00
abstract::To determine the nature and sequence of the histologic changes in the early rejection of pancreaticoduodenal allografts and to assess the correlation between pancreaticoduodenal biopsy findings and the pathologic changes in the graft, we performed serial cystoscopically directed needle biopsies of pancreaticoduodenal ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198911000-00008
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:We previously reported that posttransplant lymphoproliferative disorders (PTLD) occurred more frequently in non-African American (AF) kidney transplant recipients. An in-depth analysis of racial differences in the development of PTLD has not been reported. METHODS:We assessed Medicare claims for PTLD in a r...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182200e8a
更新日期:2011-07-27 00:00:00
abstract:BACKGROUND:It is unclear if the category of acute rejection with intimal arteritis (ARV) is relevant to short- and long-term clinical outcomes and if the graft outcomes are affected by the severity of intimal arteritis. METHODS:One hundred forty-eight ARV episodes were reviewed and categorized according to the 2013 Ba...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000640
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Impaired pretransplant performance status (PS) is associated with chronic liver disease (CLD). We studied its impact on hospital length of stay (LOS), complications and readmissions in the first year after liver transplantation. METHOD:The Standard National Liver Transplant Registry was linked to a hospital...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003484
更新日期:2020-10-08 00:00:00
abstract:BACKGROUND:The inflammation response is modulated by the elaborated chemokine-chemokine receptor system, which also plays an important role in the development of acute rejection (AR). In this study, we hypothesized that functional genetic variants of some of these modulatory proteins might influence the outcome of AR. ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000178378.53616.ca
更新日期:2005-11-15 00:00:00
abstract:BACKGROUND:Cell-based insulin therapies can potentially improve glycemic regulation in insulin-dependent diabetic patients. Enteroendocrine cells engineered to secrete recombinant insulin have exhibited glycemic efficacy, but have been primarily studied as uncontrollable growth systems in immune incompetent mice. Furth...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000247
更新日期:2014-09-15 00:00:00
abstract::FK506 (tacrolimus) is a strong immunosuppressant: it has been approved as a drug for liver transplantation in Japan, the United States, and the United Kingdom. One of its main adverse effects is hyperglycemia. Thus, in this study, we investigated the mechanism and the reversibility of the hyperglycemia caused by FK506...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-06-15 00:00:00
abstract::Sprague-Dawley rats were treated for 14 days with rapamycin (RAP; 1.5 mg/kg/day i.p.), cyclosporine (15 mg/kg/day by gavage), both drugs in combination, or appropriate drug vehicles. Hematological parameters and biochemical indices of renal and hepatic function were determined throughout the experimental period, at th...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199108000-00004
更新日期:1991-08-01 00:00:00
abstract:BACKGROUND:The UK Kidney Fast-Track Scheme (KFTS) was introduced in 2012 to identify kidneys at high risk of discard and to rapidly facilitate transplantation. A retrospective analysis of kidneys transplanted through the KFTS was undertaken. METHODS:UK Transplant Registry data were collected on deceased donor kidneys ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001860
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The role of CD20+ B cells in renal allograft rejection has been reappreciated. Importantly, recent studies suggest a relation between CD20+ B cell aggregates and poorer clinical outcome. In the present study, we attempted to confirm these early reports in a tightly controlled patient population and to differ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181860a74
更新日期:2008-09-27 00:00:00
abstract::One major limitation in pancreatic islet transplantation is availability of donor tissue. Donor shortage is exacerbated by islet apoptosis from the stresses of islet isolation and transplantation. Furthermore, the side effects of immunosuppressive drugs preclude transplants into patients whose diabetes is controlled b...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000132283.95107.9c
更新日期:2004-05-27 00:00:00
abstract::Spontaneous rupture of the liver has been described in association with many benign and malignant conditions. We report, to our knowledge, the first case of spontaneous rupture of the liver upon revascularization, requiring total hepatectomy and portocaval shunt, followed by successful retransplantation. Routine patho...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-200005270-00048
更新日期:2000-05-27 00:00:00
abstract:BACKGROUND:Recent changes in policies guiding allocation of transplant kidneys are predicted to increase sharing between distant geographic regions. The potential exists for an increase in cold ischemia time (CIT) with resulting increases in delayed graft function (DGF) and transplant-related costs (TRC). We sought to ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002309
更新日期:2019-02-01 00:00:00
abstract::After the progressive improvement in the results of organ transplantation we now face the challenge of shortage in organ supply. The decreasing number of organ retrievals performed in 1990 at our hospital has raised questions concerning loss of potential organ donors and opposition to donation by the next of kin. We i...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199207000-00015
更新日期:1992-07-01 00:00:00