Abstract:
INTRODUCTION:In clinical setting, Sidestream Dark Field (SDF) imaging has provided unprecedented insights into the gut microcirculation mainly by studying the intestinal mucosa of patients with ileostomies. Visualizing microvascular structure and function of ileal mucosa at the bedside brings unique opportunity for clinical research, particularly in critically ill patients. Several papers that were focused on intestinal microcirculation, used different methods of assessment because an accepted scoring systems does not exist so far and it is no surprise that it is rather difficult to compare the results from these studies. The present paper presents recommendations concerning specific aspects of image acquisition and proposes some parameters for the description of the intestinal microcirculation in human studies, as suggested by the participants of a round table meeting. METHODS:The round table meeting participants reviewed all relevant literature, discussed various aspects of image acquisition by SDF technology in patients with ileostomy and parameters for the description of intestinal mucosa microcirculation. Selected key conditions for high quality and reproducible image recordings were identified. To evaluate quality of intestinal microcirculation, selected parameters and scoring system were suggested and described. RESULTS:For image acquisition in ileostomies, five key points were proposed: optimal timing, optimal SDF device probe positioning, optimal stabilization, optimal number and length of acquired video recordings, and optimal avoidance of pressure artefacts. With regard to image analysis, simplified set of quantitative and qualitative parameters for the description of the intestinal mucosa microcirculation for the clinical studies has been proposed: vessels per villus, microvascular flow index, proportion of perfused villi, and borders of villi. The proposed parameters can be included in a semi-quantitative scoring system; however, this scoring system needs further validation. This simplified analysis does not require sophisticated software and can be performed manually on the video screen. CONCLUSION:We propose a simple methodology for image acquisition and suggest specific microvascular parameters to analyze SDF imaging studies of the intestinal mucosa microcirculation in patients with ileostomy. Proposed scoring system needs to be validated in further clinical studies.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Lehmann Ch,Abdo I,Kern H,Maddison L,Pavlovic D,Sharawi N,Starkopf J,Hall R,Johnson P,Williams L,Cerny V,MiDAS (Microcirculation Diagnostics and Applied Studies) group.doi
10.3233/CH-141810subject
Has Abstractpub_date
2014-01-01 00:00:00pages
137-46issue
2eissn
1386-0291issn
1875-8622pii
Q423M3675X727056journal_volume
57pub_type
杂志文章abstract::The present investigation was designed to evaluate the acute effect of aerobic exercise on oxidative stress and the flow properties of the blood. Fourteen clinically healthy subjects (7 men and 7 women aged 56+/-19 yr) underwent maximal treadmill exercise with blood samples drawn prior to and immediately after exercis...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Recently, it was shown that mesenchymal stem cells (MSCs) are capable of differentiating into endothelial cells which highlights the potential role of MSCs in neovascularization. In the present study, we investigated the paracrine factors responsible for tube formation in human adipose-tissue derived stem cells (ASCs)...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1397
更新日期:2011-01-01 00:00:00
abstract::A group of 15 chronic opioid addicts (DA) with mean age 26.5+/-7.3 years was studied by means of a rotational Contraves Low Shear 30 viscometer and the results have been compared with a control group of 19 healthy subjects. It was found that the mean whole blood viscosity values of the investigated group of heroin abu...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Although the inherited quantitative and qualitative disorders of fibrinogen are rare, in the course of time patients may develop complications including episodes of arterial and venous thrombosis. It can be useful to complete the laboratory assessment of these clinical conditions with the evaluation of the haemorheolo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180542
更新日期:2019-01-01 00:00:00
abstract::We studied the effect of mechanical forces (shear stress) on the kinetics of internalization of native LDL and ox-LDL in endothelial cell line ECV304. This study was performed by using Confocal microscopy and FRET with two carbocyanine dyes, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiO) as t...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:To assess sex-related differences in forearm skin microvascular reactivity to vascular occlusion and cardiovascular health in young healthy women and men. METHODS:203 young healthy individuals (67 men/136 women) participated in this study. Post-occlusive reactive hyperemia (PORH) in skin microcirculation fol...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180483
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the combined use of strain elastography (SE) and superb microvascular imaging (SMI) with grayscale ultrasound (US) according to the Breast Imaging Reporting and Data System (BI-RADS) classification for differentiating benign from malignant breast masses. METHODS:In total, 166 patients with 177 br...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-190693
更新日期:2020-01-01 00:00:00
abstract::The hemorheological performance of mammals and human will change with the time after the blood is withdraw from the subject. The whole blood apparent viscosity, erythrocyte deformability and aggregation of some mammals and human were measured. Our results showed that the hemorheological characteristics of mice, rats, ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Primary closure in dermatologic surgery is state of the art in small lesions at the head, but also in larger lesions at the trunk or the extremities. Microcirculatory effects on the skin blood flow near to the wound edges affected by primary closure. Forty three patients were investigated. Before and after surgery, sk...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1371
更新日期:2011-01-01 00:00:00
abstract::Red blood cells (RBC) possess a functional nitric oxide synthase (NOS) enzyme located in the cell membrane and cytoplasm. It has previously been observed that shear stress acting on RBC activates NOS and causes enhanced NO export. The aim of the present study was to investigate the physiological importance (e.g., in l...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1618
更新日期:2013-01-01 00:00:00
abstract::Clinical application of platelet-rich plasma (PRP) and stem cells has become more and more important in regenerative medicine during the last decade. However, differences in PRP preparations may contribute to variable PRP compositions with unpredictable effects on a cellular level. In the present study, we modified th...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141920
更新日期:2016-01-01 00:00:00
abstract::Radiographic contrast media can lead to drastic changes of the morphology of erythrocytes. The change of the erythrocyte morphology is associated with a decreased deformability possibly resulting from distinctions in the loss of constituents of the membrane cytoskeleton. However, it is unclear whether there is an intr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141879
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The reconstruction of metacarpal- and interphalangeal joints after severe hand injuries has been proven to be challenging. Commonly used procedures like arthrodesis, amputation or ray resection of the finger compromise the functionality of the injured finger. Especially for young patients, the restoration of...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-168100
更新日期:2016-01-01 00:00:00
abstract::Despite increasing advances in microvascular free tissue transfer, flap failures, most commonly resulting from thrombosis at the anastomotic vascular site, remain a significant concern. Although several experimental and clinical studies have been carried out, no consensus has been reached so far on the efficacy, dosag...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:10.3233/CH-2009-1220
更新日期:2009-01-01 00:00:00
abstract::Radiofrequency ablation (RFA) has shown to be a reasonable alternative for the treatment of hepatic tumors and metastases although multiple limitations remain. Cooling effects due to larger vessels can prevent complete coverage and may lead to early tumor relapse. This preliminary in vivo pig study combines the use of...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141886
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Glaucoma is currently attributed to two different possible pathogenetic mechanisms: mechanical (the ocular damage is induced by physical injury), and vascular (the ocular damage is sustained by ischemia of the optic nerve head). AIM:We considered the possibility that several anti-glaucoma drugs (beta-blocke...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVES:Although the microcirculatory dysfunctions of edema formation are well documented, the draining pattern of dermal edema lacks information. This study was to assess the potential drainage pathways of the interstitial fluid in patients with ankle edema using the anatomical and histological methods. METHODS AN...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-162057
更新日期:2016-10-05 00:00:00
abstract::This review starts with a brief description of the pioneering work done by J.R. Casley-Smith when he looked at the pathophysiological changes that occur, and transport from the blood vessels, through the interstitium to the lymphatics and lymphatic uptake, with the electron microscope. Collaboration with, and further ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The present study was to investigate the protective effects of salvianolic acids (SA) on deformability of red blood cells (RBCs) and its mechanism during the development of acute lung injury (ALI) induced by oleic acid (OA) in rabbits. 32 rabbits were randomized into four groups, normal control group, OA-treated group...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Increased RDW has been found to be a marker of adverse outcomes in cardiovascular disease (CVD), although the exact mechanism remains unclear. Recently, several authors have found that higher RDW is associated with decreased erythrocyte deformability, which can impair blood flow through microcirculation, a fact which ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131751
更新日期:2015-01-01 00:00:00
abstract::Hypercoagulable states due either to inherited or acquired thrombotic risk factors are only present in approximately half of cases of DVT, but the causes in the other half, remain unknown. The importance of biological risk factors such as hyperlipidemia, hypofibrinolysis and hemorheological alterations in the pathogen...
journal_title:Clinical hemorheology and microcirculation
pub_type:
doi:
更新日期:2002-01-01 00:00:00
abstract:INTRODUCTION:The hormones erythropoietin (EPO) and thrombopoietin (TPO) are main regulators of erythro- and thrombopoiesis. Cell loss caused by operative procedures may alter serum levels of the hormones, resulting in well known phenomenons like reactice thrombocytosis. MATERIAL AND METHODS:Blood samples from 10 patie...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1489
更新日期:2011-01-01 00:00:00
abstract::Blood rheology is a well-known determinant of tissue perfusion and, according to the Poiseuille relation, hemodynamic resistance in a constant-geometry vascular network is directly proportional to blood viscosity. However, this direct relationship cannot be observed in all in vivo studies. Further, there are several r...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Capsular contractures around breast implants usually develop leading to pain and aesthetically inadequate results and ultimately often requires the replacement of the implants. Textured silicone implants are the most commonly placed implant, but polyurethane-coated implants are increasingly being used in an ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200875
更新日期:2020-09-08 00:00:00
abstract::Autologus veins have been used clinically as a bypass conduit for reconstruction of small arteries, but there are few data available for microvascular response to arteriovenous (AV) shunting. This study was aimed to evaluate microvascular hemodynamic changes induced by creating AV anastomosis in rat hindlimb. Using in...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:The interleukin-1 receptor antagonist (IL-1RA) contributes to tumor survival and progression in multiple cancer entities. IL-1RA polymorphisms influence IL-1RA expression patterns and function. A known polymorphism was correlated with clinical outcomes in melanoma patients with particularly aggressive disease...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-179212
更新日期:2017-01-01 00:00:00
abstract::In Chinese traditional medicine, angelica is widely used for its known clinical effects of ameliorating blood microcirculation. But the mechanism of these beneficial effects still remains unclear. In this work the rheological behaviour of human erythrocytes treated by angelica was studied in vitro. Normal RBCs incubat...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND AIM:Hematocrit is an important hemorheological parameter. Both hematocrit and nonalcoholic fatty liver disease (NAFLD) are strongly correlated with cardiovascular disease. However, there is no study to explore the direct relationship of hematocrit with NAFLD. METHODS:Hematocrit levels were analyzed from...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1509
更新日期:2012-01-01 00:00:00
abstract::It is well established that exercise performance in athletes is related to improved blood fluidity. However, training effects on functional state of endothelium and relations of endothelial cell functions with hemorheology are poorly known. Circulating levels of von Willebrand factor may serve as a good marker of endo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:To identify the indications for CEUS in renal imaging in an interdisciplinary ultrasound department. METHODS:102 CEUS examinations of the kidney in 82 patients between September 2014 and July 2016 were analysed regarding the indication for ultrasound. CEUS was performed by one experienced sonographer agent a...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-179103
更新日期:2017-01-01 00:00:00