当前位置： SCI文献检索 > LIFE SCIENCES期刊下所有文献 > Estrogen modulates the inducible expression of platelet-derived growth factor mRNA by monocyte/macrophages.

Estrogen modulates the inducible expression of platelet-derived growth factor mRNA by monocyte/macrophages.

Abstract：

:We examined the effects of estrogen, 12-O-tetradecanoylphorbol 13 acetate (TPA), and lipopolysaccharide (LPS) on the gene expression of platelet-derived growth factor (PDGF) by the monocyte/macrophage cell line, THP-1. THP-1 cells were exposed to TPA for 48 or 96 hours to induce differentiation. Some were treated with LPS in the last 3 hours and/or ethinyl estradiol (estrogen) (10(-9) M) in the last 20 hours. Total cellular RNA was isolated and cDNA was synthesized and then coamplified (with an internal control, beta-actin, product size 1126 bp) using polymerase chain reaction (PCR) and a set of primers for PDGF-A (product size 225 bp), PDGF-B (217 bp), or PDGF beta-receptor (PDGF-R) (228 bp). The products were separated on an agarose gel and the ratios of radioactivity incorporated into PDGF PCR products to beta-actin products were used to assess the relative changes in the levels of PDGF mRNA abundance in response to various inducers. TPA induced the expression of PDGF-A mRNA, whereas LPS had no effect. Treatment of TPA-stimulated cells with estrogen caused a 61% and 190% increase in PDGF-A mRNA (p < 0.05) at 48 and 96 hours, respectively. Addition of estrogen to cells treated with both TPA and LPS did not cause any significant change in the amounts of the transcripts. In contrast to PDGF-A mRNA, attempts to visualize and estimate PDGF-B and PDGF-R mRNA were unsuccessful. This was probably due to low levels of these transcripts in THP-1 cells. The results indicate that estrogen modulates PDGF-A gene expression by monocyte/macrophages and suggest that estrogen may influence atherogenesis at the vascular level.

journal_name

Life Sci

journal_title

Life sciences

authors

Shanker G,Sorci-Thomas M,Adams MR

doi

10.1016/0024-3205(94)00479-c

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

499-507

issue

7

eissn

0024-3205

issn

1879-0631

pii

002432059400479C

journal_volume

56

pub_type

杂志文章

相关文献

LIFE SCIENCES文献大全
  • The effect of taurine on cholesterol degradation in mice fed a high-cholesterol diet.

    abstract：:The hypocholesterolemic effect of taurine was examined in mice fed a high-cholesterol diet containing 1% cholesterol and 0.25% sodium cholate. Male C57BL/6 mice were divided into 3 groups: control group (HC), 1% taurine-supplemented group (HCT+), and taurine-deficient group (HCT-) produced by supplying 0.5% guanidinoe...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2003.08.041

    authors： Chen W,Matuda K,Nishimura N,Yokogoshi H

    更新日期：2004-02-27 00:00:00

  • Leptin changes Ca2+/calmodulin-dependent response and up-regulates the gene expression of calcineurin in rat hypothalamus.

    abstract：:To understand the leptin's action on hypothalamic arcuate nucleus (ARC), Ca2+/calmodulin (CaM)-dependent response in leptin-treated rat ARC was investigated. The in vitro phosphorylation of ARC extracts from leptin-treated rats did not respond to CaM. Although CaM-dependent protein kinase II activity was not affected ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(98)00460-3

    authors： Morishita T,Hidaka T,Sugahara K,Noguchi T

    更新日期：1998-01-01 00:00:00

  • Mapping catechol-O-methyltransferase in vivo: initial studies with [18F]Ro41-0960.

    abstract：:Ro41-0960 is a potent, fluorine containing COMT inhibitor which has be en reported to cross the blood brain barrier and to inhibit COMT in the brain. It is structurally similar to Ro40-7592 which is currently undergoing clinical trials in Parkinson's disease. Positron emission tomographic (PET) studies in baboon using...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(95)02277-5

    authors： Ding YS,Gatley SJ,Fowler JS,Chen R,Volkow ND,Logan J,Shea CE,Sugano Y,Koomen J

    更新日期：1996-01-01 00:00:00

  • Effects of losartan on the blood-brain barrier permeability in long-term nitric oxide blockade-induced hypertensive rats.

    abstract：:Hypertension is closely associated with vascular endothelial dysfunction. The aim of this study was to investigate the effects of Angiotensin II (ANG II) receptor antagonist losartan on the blood-brain barrier (BBB) permeability in L-NAME-induced hypertension and/or in ANG II-induced acute hypertension in normotensive...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(02)01772-1

    authors： Kucuk M,Kaya M,Kalayci R,Cimen V,Kudat H,Arican N,Elmas I,Korkut F

    更新日期：2002-07-12 00:00:00

  • Pharmacological characterisation of voltage-dependent Ca2+ channels in isolated ganglia from the myenteric plexus.

    abstract：:Voltage-dependent Ca2+ channels of fura-2-loaded ganglionic cells from the myenteric plexus of newborn rats were pharmacologically characterised. In contrast to completely dissociated myenteric cells, intact ganglia showed a stronger loading with the Ca2+-sensitive dye and a reproducible stimulation of the fura-2 sign...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2004.12.053

    authors： Schäufele N,Diener M

    更新日期：2005-09-30 00:00:00

  • Effect of fish oil and vitamin E on ethanol-induced changes in membrane ATPases.

    abstract：:We evaluated whether fish oil or vitamin E administration affected ethanol-induced changes in membrane ATPases. Male Wistar rats (225-250 g) were fed, through a gastric tube a liquid diet containing fish oil (25% of calories) and ethanol for one month. Another group of animals was given supplemental vitamin E (300 u/k...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(94)00545-1

    authors： Nanji AA,Sadrzadeh SM

    更新日期：1994-01-01 00:00:00

  • A multicenter, randomized, placebo controlled, multiple-dose, safety and pharmacokinetic study of AIT-082 (Neotrofin) in mild Alzheimer's disease patients.

    abstract：:A phase 1, randomized, double-blind, placebo-controlled, dose escalation study of the purine derivative, AIT-082 (Neotrofin, NeoTherapeutics) was conducted in mild Alzheimer's disease (AD) patients to evaluate multiple-dose safety, tolerability, and pharmacokinetics. Possible short-term effects of AIT-082 on cognition...

    journal_title：Life sciences

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0024-3205(03)00320-5

    authors： Grundman M,Capparelli E,Kim HT,Morris JC,Farlow M,Rubin EH,Heidebrink J,Hake A,Ho G,Schultz AN,Schafer K,Houston W,Thomas R,Thal LJ,Alzheimer's Disease Cooperative Study.

    更新日期：2003-06-20 00:00:00

  • Hydroperoxide and cigarette smoke induced effects on lung mechanics and glutathione status in rat isolated perfused and ventilated lungs.

    abstract：:The effects of t-butylhydroperoxide (TBH) and cigarette smoke on lung mechanics (CDYN and RL) and glutathione status (GSH) were studied using an isolated perfused and ventilated rat lung preparation. TBH (200, 400, 1000 microM) infused via the pulmonary circulation caused a dose-related bronchoconstriction. The lung G...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(88)90054-9

    authors： Ryrfeldt A,Kröll F,Berggren M,Moldéus P

    更新日期：1988-01-01 00:00:00

  • Protease inhibitors protect macrophages from lipopolysaccharide-induced cytotoxicity: possible role for NF-kappaB.

    abstract：:Recent studies suggest lipopolysaccharide (LPS) mediated cell death as underlying mechanism of hyporesponsiveness and dysfunction of macrophages in the late phase of septic shock. In the present study LPS (0.001 - 30 microg/ml) caused a concentration-dependent toxicity in the macrophage cell line (J774.1A) within 24 h...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(98)00031-9

    authors： Abate A,Schröder H

    更新日期：1998-01-01 00:00:00

  • FcγRI (CD64) contributes to the severity of immune inflammation through regulating NF-κB/NLRP3 inflammasome pathway.

    abstract：AIMS:Fcγ receptor I (FcγRI/CD64) that is restrictedly expressed on monocytes and macrophages, acts as the single high-affinity receptor of immunoglobulin G (IgG) in human. The expression of FcγRI is positively correlated with immune inflammation. The primary aim of this study was to explore the effects of FcγRI express...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2018.06.015

    authors： Zhang H,Li L,Liu L

    更新日期：2018-08-15 00:00:00

  • Spectral and inhibitory interactions of (+/-)-3,4-methylenedioxyamphetamine (MDA) and (+/-)-3,4-methylenedioxymethamphetamine (MDMA) with rat hepatic microsomes.

    abstract：:Incubation of racemic methylenedioxyamphetamine (MDA) or methylenedioxymethamphetamine (MDMA) with rat hepatic microsomes, in the presence of NADPH, generated a spectrally observed inhibitory complex with cytochrome P-450. The complex inhibited product formation from MDA and MDMA as well as other P-450 dependent react...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(86)90551-5

    authors： Brady JF,Di Stefano EW,Cho AK

    更新日期：1986-10-20 00:00:00

  • Prostaglandin E2 upregulates survivin expression via the EP1 receptor in hepatocellular carcinoma cells.

    abstract：AIMS:Cyclooxygenase-2 (COX-2)-controlled production of prostaglandin E(2) (PGE(2)) has been implicated in cell growth and metastasis in many cancers. Recent studies have found that COX-2 is co-expressed with survivin in many cancers. Survivin is a member of the inhibitor-of-apoptosis protein family. Some COX-2 inhibito...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2009.12.009

    authors： Bai XM,Jiang H,Ding JX,Peng T,Ma J,Wang YH,Zhang L,Zhang H,Leng J

    更新日期：2010-01-30 00:00:00

  • Depressive and anxiety symptoms in the early puerperium are related to increased degradation of tryptophan into kynurenine, a phenomenon which is related to immune activation.

    abstract：:There is now some evidence that i) the availability of plasma tryptophan, the precursor of serotonin, is significantly lower in pregnant women at the end of term and the first few days after delivery than in nonpregnant women; and ii) both pregnancy and the early puerperium are accompanied by activation of the inflamm...

    journal_title：Life sciences

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0024-3205(02)01853-2

    authors： Maes M,Verkerk R,Bonaccorso S,Ombelet W,Bosmans E,Scharpé S

    更新日期：2002-09-06 00:00:00

  • Pharmacological properties of P2 receptors on rat otic parasympathetic ganglion neurons.

    abstract：:To elucidate the pharmacological profile of P2X receptors and the probable expression of P2Y receptors in otic ganglion neurons from 17-day-old rats, single neurons were enzymatically isolated and maintained in tissue culture for up to 30 h. Whole-cell voltage-clamp recording was carried out at a holding potential of ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2008.06.005

    authors： Ma B,Yu LH,Fan J,Ni X,Burnstock G

    更新日期：2008-08-01 00:00:00

  • Prenatal exposure to low levels of carbon monoxide alters sciatic nerve myelination in rat offspring.

    abstract：:Prenatal exposure to low concentrations of carbon monoxide (CO, 75 and 150 ppm from day 0 to day 20 of gestation), resulting in maternal blood HbCO concentrations equivalent to those maintained by human cigarette smokers, leads to subtle myelin alterations in the sciatic nerve of male rat offspring. The rapid growth s...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(00)00761-x

    authors： Carratù MR,Cagiano R,Desantis S,Labate M,Tattoli M,Trabace L,Cuomo V

    更新日期：2000-08-25 00:00:00

  • The dynamic relationship between mu and kappa opioid receptors in body temperature regulation.

    abstract：:Previous studies demonstrated that intracerebroventricular (icv) injection of a kappa opioid receptor agonist decreased, and a mu agonist increased, body temperature (Tb) in rats. A dose-response study with the selective kappa antagonist nor-binaltorphimine (nor-BNI) showed that a low dose (1.25 nmol, icv) alone had n...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2005.04.084

    authors： Chen X,McClatchy DB,Geller EB,Tallarida RJ,Adler MW

    更新日期：2005-12-12 00:00:00

  • Myricetin inhibits the induction of anti-Fas IgM-, tumor necrosis factor-alpha- and interleukin-1beta-mediated apoptosis by Fas pathway inhibition in human osteoblastic cell line MG-63.

    abstract：:The survival of osteoblast cells is one of the determinants of the development of osteoporosis in patients with inflamed synovium, such as in rheumatoid arthritis (RA). By means of alkaline phosphatase (ALP) activity and osteocalcin ELISA assay, I have shown that myricetin exhibits a significant induction of different...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2005.05.026

    authors： Kuo PL

    更新日期：2005-10-21 00:00:00

  • 5 alpha-reduced progesterone metabolites are essential in hamster VTA for sexual receptivity.

    abstract：:Progesterone's (P) actions in the ventral medial hypothalamus (VMH) and the ventral tegmental area (VTA) are essential for sexual receptivity in hamsters. This study investigates the notion that P works in the VTA in the absence of intracellular P receptors (PRs) by 5 alpha-reduction to progestins, which would subsequ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(94)00548-6

    authors： Frye CA,Leadbetter EA

    更新日期：1994-01-01 00:00:00

  • Evolution of the activity of UGT1A1 throughout the development and adult life in a rat.

    abstract：:Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2005.08.018

    authors： Bustamante N,Cantarino MH,Arahuetes RM,Cubero FJ,Ortiz A

    更新日期：2006-03-06 00:00:00

  • Overexpression of uncoupling protein 4 promotes proliferation and inhibits apoptosis and differentiation of preadipocytes.

    abstract：:Uncoupling proteins are a family of mitochondrial proteins involved in energy metabolism. We previously showed that uncoupling protein 4 (UCP4) is differentially expressed in omental adipose tissue in diet-induced obese and normal rats. However, the effect of UCP4 on adipocytes is unclear. In this work, we established...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2006.04.012

    authors： Zhang M,Wang B,Ni YH,Liu F,Fei L,Pan XQ,Guo M,Chen RH,Guo XR

    更新日期：2006-09-05 00:00:00

  • Lack of biological activity of preproendothelin [110-130] in several endothelin assays.

    abstract：:A 21 amino acid peptide containing the prepropendothelin sequence from amino acids 110 to 130 and two intrachain disulfide bonds was synthesized and tested for biological activity in the following endothelin assays: 1.) a competition binding assay using [125I]ET-1 and dog heart membranes, 2.) three RIA's using 125I-ET...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90308-e

    authors： Cade C,Lumma WC Jr,Mohan R,Rubanyi GM,Parker-Botelho LH

    更新日期：1990-01-01 00:00:00

  • Intra-arterial transplantation of human umbilical cord blood mononuclear cells in neonatal hypoxic-ischemic rats.

    abstract：UNLABELLED:Based on preclinical findings, cellular therapy has become a promising therapeutic approach for neonatal hypoxia-ischemia (HI). However, before translation into the clinical setting, new and effective routes of cell delivery must be determined. Intra-arterial (IA) delivery is an attractive route of cellular ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2013.10.017

    authors： Greggio S,de Paula S,Azevedo PN,Venturin GT,Dacosta JC

    更新日期：2014-02-06 00:00:00

  • Biochemical characterization of serotonin stimulated phosphoinositide turnover.

    abstract：:Serotonin (5HT) stimulates phosphoinositide turnover in a number of tissues, but it is not known whether this effect is due to activation of a 5HT receptor which is coupled to phosphoinositide hydrolysis or if the effect is secondary to 5HT stimulated arachidonate metabolism or to the release of another neurotransmitt...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(86)90060-3

    authors： Conn PJ,Sanders-Bush E

    更新日期：1986-02-17 00:00:00

  • Non-right sidedness: an association with lower IL-2 production.

    abstract：:Using a laterality questionnaire, 138 normal healthy individuals were classified as right-sided and 25 as non-right sided. Interleukin-2 (IL-2) was generated from whole blood obtained from these subjects using mitogen (PHA) stimulation. IL-2 was quantitated in picograms/ml using an enzyme immunoassay (ELISA). Addition...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(92)90035-n

    authors： Chengappa KN,Ganguli R,Yang ZW,Schurin G,Cochran J,Brar JS,Rabin B

    更新日期：1992-01-01 00:00:00

  • Tumor necrosis factor-alpha-mediated signal transduction in human neutrophils: involvement of sphingomyelin metabolites in the priming effect of TNF-alpha on the fMLP-stimulated superoxide production.

    abstract：:We investigated the mechanism underlying the priming effect of TNF-alpha on fMLP-stimulated superoxide production in human neutrophils. TNF-alpha enhanced fMLP-stimulated superoxide production in a concentration-dependent manner. TNF-alpha also induced sphingomyelin (SM) hydrolysis and increased the formation of its m...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(99)00587-1

    authors： Niwa M,Kozawa O,Matsuno H,Kanamori Y,Hara A,Uematsu T

    更新日期：2000-01-01 00:00:00

  • Delay of puberty and impairment of growth in female rats given a non competitive antagonist of NMDA receptors.

    abstract：:Reportedly, excitatory amino acids are involved in the control of gonadotropin secretion of rats and non-human primates. The aim of this study was to investigate the effect of chronic blockade of NMDA (N-methyl-D-aspartic acid) receptors by the non competitive receptor antagonist MK-801 on gonadotropin secretion and t...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90218-g

    authors： Veneroni O,Cocilovo L,Müller EE,Cocchi D

    更新日期：1990-01-01 00:00:00

  • Tissue distribution of phenoxybenzamine in the rat. Lack of adipose tissue storage.

    abstract：:Rats were given the basic lipophilic drug, phenoxybenzamine, in single i.v. doses of 0.4 and 30 mg/kg. The drug was determined in plasma and 7 tissues by a new HPLC method. Adipose tissue reached peak levels after 30 minutes. At that time levels in heart, kidney, and brain were higher than in subcutaneous, epididymal,...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(87)90478-4

    authors： Moor MJ,Bickel MH

    更新日期：1987-10-26 00:00:00

  • Differentially expressed CC3/TIP30 and rab11 along in vivo and in vitro intestinal epithelial cell crypt-villus axis.

    abstract：:We have previously shown that transforming growth factor-beta1 (TGF-beta1) is involved in the fibroblast-induced organization and differentiation of transformed phenotypically crypt-like T84 intestinal epithelial cells into absorptive enterocyte-like cells, when cultured within a three-dimensional collagen gel. We hav...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(01)01216-4

    authors： Lindfors K,Halttunen T,Kainulainen H,Mäki M

    更新日期：2001-08-10 00:00:00

  • Prevention of immunosuppression in stressed mice by neurotropin(NSP).

    abstract：:It is well known that the immune function can be compromised by stress. To investigate immune function in mice stressed by experimental restraint or unavoidable and opioid dependent stress, we evaluated the changes in total body weight and in organ weights (liver, spleen and thymus) of these animals, as well as the ph...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90600-v

    authors： Teshima H,Sogawa H,Kihara H,Kubo C,Mori K,Nakagawa T

    更新日期：1990-01-01 00:00:00

  • Multi-omics analysis of copy number variations of RNA regulatory genes in soft tissue sarcoma.

    abstract：AIMS:RNA regulatory genes were closely associated with tumorigenesis and prognosis in multiple tumors. Copy number variation (CNV) is a frequent characteristic in soft tissue sarcomas (STS). However, little is known regarding their possible roles in STS. MAIN METHODS:RNA sequence profiles and CNV data of 255 STS patie...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.118734

    authors： Wang X,Han Y,Li J,Hong D,Xue Z,Huang H,Du Z,Hou Y,Li H,Li H,Liao H,Xie X,Ding C

    更新日期：2021-01-15 00:00:00