Abstract:
:We have previously demonstrated that, in prostate cancer cells, androgens up-regulate IGF-I receptor (IGF-IR) by inducing cAMP-response element-binding protein (CREB) activation and CREB-dependent IGF-IR gene transcription through androgen receptor (AR)-dependent membrane-initiated effects. This IGF-IR up-regulation is not blocked by classical antiandrogens and sensitizes cells to IGF-I-induced biological effects. Metformin exerts complex antitumoral functions in various models and may inhibit CREB activation in hepatocytes. We, therefore, evaluated whether metformin may affect androgen-dependent IGF-IR up-regulation. In the AR(+) LNCaP prostate cancer cells, we found that metformin inhibits androgen-induced CRE activity and IGF-IR gene transcription. CRE activity requires the formation of a CREB-CREB binding protein-CREB regulated transcription coactivator 2 (CRTC2) complex, which follows Ser133-CREB phosphorylation. Metformin inhibited Ser133-CREB phosphorylation and induced nuclear exclusion of CREB cofactor CRTC2, thus dissociating the CREB-CREB binding protein-CRTC2 complex and blocking its transcriptional activity. Similarly to metformin action, CRTC2 silencing inhibited IGF-IR promoter activity. Moreover, metformin blocked membrane-initiated signals of AR to the mammalian target of rapamycin/p70S6Kinase pathway by inhibiting AR phosphorylation and its association with c-Src. AMPK signals were also involved to some extent. By inhibiting androgen-dependent IGF-IR up-regulation, metformin reduced IGF-I-mediated proliferation of LNCaP cells. These results indicate that, in prostate cancer cells, metformin inhibits IGF-I-mediated biological effects by disrupting membrane-initiated AR action responsible for IGF-IR up-regulation and suggest that metformin could represent a useful adjunct to the classical antiandrogen therapy.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Malaguarnera R,Sacco A,Morcavallo A,Squatrito S,Migliaccio A,Morrione A,Maggiolini M,Belfiore Adoi
10.1210/en.2013-1925subject
Has Abstractpub_date
2014-04-01 00:00:00pages
1207-21issue
4eissn
0013-7227issn
1945-7170journal_volume
155pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The CYP11A1 encodes cytochrome P450scc, catalyzing the first step of steroidogenesis in adrenals and gonads under the control of cAMP-mediated hormonal signals. The cAMP-induced activation of human CYP11A1 has been suggested to depend on the transcription factor cAMP-responsive element-binding protein (CREB), but the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0938
更新日期:2007-04-01 00:00:00
abstract::In the Syrian hamster, a sharp peak in pineal melatonin occurs toward the end of the dark period. In the present communication, we describe characteristics of this rhythm. First, the time of the initial increase in pineal melatonin is not altered by daily melatonin injections that induce gonadal regression. Second, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-5-1525
更新日期:1980-11-01 00:00:00
abstract::Serum levels of progesterone in hysterectomized gilts were determined to evaluate the effects of exogenous 17 beta-estradiol (17 beta-E2) on the secretory activity of aging corpora lutea. Gilts were hysterectomized on day 6 of the estrous cycle and given im injections of 17 beta-E2 to mimic blood levels of endogenous ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-5-1876
更新日期:1984-11-01 00:00:00
abstract::The nature and role of the peptidergic innervation of the ovary were examined by determining the location and function of vasoactive intestinal peptide (VIP)-containing nerve fibers in the immature rat ovary. Immunohistofluorescence analysis of prepubertal ovaries using a specific VIP antibody revealed sparse delicate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1682
更新日期:1986-04-01 00:00:00
abstract::The enteroendocrine and enteric nervous systems convey signals through an overlapping network of regulatory peptides that act either as circulating hormones or as localized neurotransmitters within the gastrointestinal tract. Because recent studies invoke an important role for vasoactive intestinal peptide (VIP) as a ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1069
更新日期:2012-06-01 00:00:00
abstract::Nowadays, we are bombarded with information on a large number of endocrine-disrupting chemicals. We hear and read about endocrine-disrupting chemicals on blogs, the web, news stories, television specials, advertisements, and of course scientific articles. Reports claim these ubiquitous compounds are responsible for in...
journal_title:Endocrinology
pub_type: 历史文章,杂志文章
doi:10.1210/en.2016-1445
更新日期:2016-08-01 00:00:00
abstract::A study was conducted to determine the role of the thyroid gland in three neuroendocrine responses to photoperiod; secretion of melatonin, PRL, and LH. Ewes were thyroidectomized (THX) in midsummer or left thyroid intact, and both groups were moved indoors to artificial short days (8 h of light, 16 h of darkness) for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013340
更新日期:1994-07-01 00:00:00
abstract::PTH increases calcium reabsorption exclusively in the distal nephron. Two mechanisms of Ca++ transport through the basolateral membrane (BLM) have been described: the ATP-dependent and the sodium gradient-dependent transport. In the present study, we investigated the effect of PTH and (Bu)2cAMP on these two mechanisms...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-251
更新日期:1991-01-01 00:00:00
abstract::The lactogen-dependent rat Nb2 lymphoma is a useful model to investigate PRL signaling pathways that lead to regulation of gene transcription. A primary mechanism coupled to PRL receptor (PRLR) activation in Nb2 cells involves phosphorylation by Jak-family tyrosine kinases of one or more signal transducers and activat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7215
更新日期:1999-12-01 00:00:00
abstract::The influence of sex hormones on the immune response to foreign antigens as well as self-antigens is now recognized. In this study, we investigated the influence of gender and sex hormones on the development of antibodies to double-stranded DNA in nonautoimmune C57BL/6J mice. Immunoglobulin G (IgG) anti-dsDNA antibodi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988450
更新日期:1994-12-01 00:00:00
abstract::Angiotensin II and extracellular potassium stimulate aldosterone production in adrenal glomerulosa cells by mobilizing the calcium messenger system. This response requires calcium influx across the plasma membrane, followed by calcium uptake into the mitochondria. It has been proposed that calcium is transported to th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0268
更新日期:2003-10-01 00:00:00
abstract::The pigeon crop has long been known as a target tissue for PRL action. This study investigates the molecular events associated with PRL stimulation of the pigeon crop sac. Several experiments were performed comparing isolates from PRL-injected pigeons (200 micrograms/day, im, for 3 days) to saline-treated pigeons. Ele...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1718
更新日期:1984-05-01 00:00:00
abstract::Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermatogenesis. It also prevents Leydig cells (LCs) from gonadotropin overstimulation of androgen production. In transgenic (Tg) mice carrying deletions...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2230
更新日期:2013-06-01 00:00:00
abstract::Because there are sex differences in feeding behavior in rats, we looked for a possible sex difference in the response to glucose of melanin-concentrating hormone (MCH) neurons in the lateral hypothalamic area using phosphorylated cAMP response element-binding protein (pCREB) as a marker of neural activity. Intact mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0078
更新日期:2005-08-01 00:00:00
abstract::Japanese quail, a species with a precocial pattern of development, were used as a model for studying the ontogeny of thyroid responses to TSH. Thiourea was administered to embryos early in incubation, and the effects were assessed by measuring thyroid 32P uptake, serum thyroid hormones, thyroid weights, and body weigh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1238
更新日期:1984-04-01 00:00:00
abstract::Embryonic poly(A)-binding protein (EPAB) is the predominant poly(A)-binding protein in Xenopus, mouse, and human oocytes and early embryos before zygotic genome activation. EPAB is required for translational activation of maternally stored mRNAs in the oocyte and Epab(-/-) female mice are infertile due to impaired ooc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1135
更新日期:2016-01-01 00:00:00
abstract::Apoptosis is the process of cellular self-destruction, and genes such as bcl-2 and bax are known to inhibit and promote apoptosis, respectively. In this study, we show that apoptosis can be induced in pancreatic beta-cell lines, and we investigate the apoptotic pathways through the bcl-2 and bax genes and intracellula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5798
更新日期:1998-03-01 00:00:00
abstract::It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0484
更新日期:2010-12-01 00:00:00
abstract::In seasonally breeding species, an animal's photoperiodic history (the daylength or photoperiod previously experienced) influences the reproductive response to new photoperiods. However, this has only been examined over a relatively narrow range of photoperiods. We assessed whether Djungarian hamsters (Phodopus sungor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2634
更新日期:1988-06-01 00:00:00
abstract::Ovarian angiotensin I (Ang I)-converting enzyme (ACE), estimated by the specific binding of the ACE inhibitor [125I]iodo-MK-351A, is localized on multiple ovarian structures, including follicular granulosa cells, corpora lutea, terminal epithelium, and ovarian blood vessels, but total ovarian ACE does not display a cy...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-2927
更新日期:1990-06-01 00:00:00
abstract::SH2-containing inositol 5'-phosphatase 2 (SHIP2) is a 5'-lipid phosphatase hydrolyzing the phosphatidylinositol (PI) 3-kinase product PI(3,4,5)P(3) to PI(3,4)P(2) in the regulation of insulin signaling, and is shown to be increased in peripheral tissues of diabetic C57BL/KSJ-db/db mice. To clarify the impact of SHIP2 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0820
更新日期:2008-02-01 00:00:00
abstract::The present experiments were devoted to analyzing the hypothesis that somatostatin (SS) could modulate glomerular filtration rate by interacting with mesangial cells. Studies were performed in cultured human mesangial cells, passages 3-5. Radioligand experiments demonstrated the presence in the cells of two kinds of r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628380
更新日期:1995-08-01 00:00:00
abstract::LHRH release is dependent on the availability of calcium, and prostaglandin E2 is a potent releaser of LHRH. Therefore, we investigated the role of phospholipase A2 (PLA2) on the release of LHRH from the hypothalamus. Four rat hypothalami were perifused with Krebs-Ringer buffer, and after a 60-min preincubation period...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-4-1483
更新日期:1987-10-01 00:00:00
abstract::The uterotropic response of the uterus to 17β-estradiol (E2) is genetically controlled, with marked variation observed depending on the mouse strain studied. Previous genetic studies from our laboratory using inbred mice that are high (C57BL6/J; B6) or low (C3H/HeJ; C3H) responders to E2 led to the identification of q...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1910
更新日期:2014-08-01 00:00:00
abstract::We used autoradiography to test the hypothesis that a major function of thyroid hormone-binding proteins in plasma is to ensure uniform distribution of thyroid hormones among cells of a given tissue. The distribution of [125I]T4 within rat hepatic lobules was determined after its single pass perfusion through the port...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1742
更新日期:1987-05-01 00:00:00
abstract::Leptin, a recently-discovered hormonal product of the obese gene, circulates in the blood at levels paralleling those of fat reserves and regulates satiety and improves reproductive performance if injected into mice lacking circulating leptin. Therefore, we tested the hypothesis that leptin signals metabolic informati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5311
更新日期:1997-08-01 00:00:00
abstract::The present study investigates the effect of different endocrine manipulations on the gamma-aminobutyric acid (GABA)-ergic system in the rat fallopian tube. Either hypophysectomy or ovariectomy induced a significant decrease of glutamic acid decarboxylase (GAD) activity and of GABA levels in in situ tubes. This effect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-334
更新日期:1986-01-01 00:00:00
abstract::Magnocellular hypothalamic neurons in the rat supraoptic nucleus (SON) normally produce either vasopressin (VP) or oxytocin (OT). Here we demonstrate that many magnocellular neurons in the SON of lactating rats synthesize both hormones at the same time. We show the colocalization of the messenger (m) RNA that encodes ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1814
更新日期:1991-10-01 00:00:00
abstract::Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ET(A)R) in murine models of thyroid carcinoma using optical imaging methods. A...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-2017
更新日期:2012-06-01 00:00:00
abstract::In agonadal juvenile male monkeys, continuous administration of human metastin 45-54 (hu metastin 45-54) leads to desensitization of its receptor, G protein-coupled receptor 54 (GPR54), and decreased LH. The present study extended this observation to the adult male monkey, a more preclinically relevant model in which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0207
更新日期:2007-07-01 00:00:00