Abstract:
:Vaccinia virus maturation into infectious particles appears to be dependent on the proteolytic processing of at least five viral proteins, each containing a conserved AG*X cleavage motif and each requiring proper association with the previrion particle. To identify the responsible proteinase, a transcriptionally controlled trans-processing assay was developed to monitor cleavage at the permissive AG*S site of the P25K core protein precursor. This assay led to the putative identification of a VV proteinase encoded by open reading frame G1L. The predicted protein contains an HXXEH sequence which is a direct inversion of the active site consensus sequence present in thermolysin and other metalloendopeptidases. Site-directed mutation of this consensus sequence suggests that the G1L protein may be a novel, virus-encoded metalloendoproteinase, although confirmation of this activity must await the development of a suitable cell-free processing assay.
journal_name
J Viroljournal_title
Journal of virologyauthors
Whitehead SS,Hruby DEdoi
10.1128/JVI.68.11.7603-7608.1994subject
Has Abstractpub_date
1994-11-01 00:00:00pages
7603-8issue
11eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Recombinant interleukin-16 (rIL-16) has been found to inhibit human immunodeficiency virus type 1 (HIV-1) replication in acutely or endogenously infected CD4(+) T cells. However, the effect of rIL-16 on HIV-1 replication in antigen-presenting cells (APCs) is still unknown. We show here a potent HIV-suppressive activit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.7008-7013.1999
更新日期:1999-08-01 00:00:00
abstract::Hepatitis C virus (HCV) reorganizes intracellular membranes to establish sites of replication. How viral and cellular proteins target, bind, and rearrange specific membranes into the replication factory remains a mystery. We used a lentivirus-based RNA interference (RNAi) screening approach to identify the potential c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06001-11
更新日期:2012-02-01 00:00:00
abstract::Herpes simplex virus 1 replication initiates angiogenesis and inflammation in the cornea. This can result in herpetic stromal keratitis (HSK), which is a leading cause of infection-induced corneal blindness. Host cellular factors mediate the progression of HSK, but little is known about these cellular factors and thei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-12
更新日期:2012-08-01 00:00:00
abstract::Determinants of glycosylation site usage were explored by using the hemagglutinin-neuraminidase (HN) glycoprotein of the paramyxovirus Newcastle disease virus. The amino acid sequence of the HN protein, a type II glycoprotein, has six N-linked glycosylation addition sites, G1 to G6, two of which, G5 and G6, are not us...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3083-3089.1997
更新日期:1997-04-01 00:00:00
abstract::Like almost all of the positive-strand RNA viruses, hepatitis C virus (HCV) induces host intracellular membrane modification to form the membrane-bound viral replication complex (RC), within which viral replicases amplify the viral RNA genome. Despite accumulated information about how HCV co-opts host factors for vira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01140-16
更新日期:2016-10-14 00:00:00
abstract::Simian virus 40 (SV40) large T antigen (Tag) is a promiscuous transcriptional transactivator; however, its mechanism of transactivation remains unknown. Recent studies have suggested the possible involvement of protein-protein interactions with TBP, the TATA box-binding protein of TFIID, and TEF-1, an enhancer-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1191-1202.1996
更新日期:1996-02-01 00:00:00
abstract::Previous work on the strict late (gamma) UL38 promoter of herpes simplex virus type 1 identified three cis-acting elements required for wild-type levels of transcription: a TATA box at -31, a consensus mammalian initiator element at the transcription start site, and a downstream activation sequence (DAS) at +20 to +33...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8181-8190.1998
更新日期:1998-10-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in infants and the elderly, but no safe and effective RSV vaccine is yet available. For reasons that are not well understood, RSV is only weakly immunogenic, and reinfection occurs throughout life. This has complicated the sea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1130-1139.2006
更新日期:2006-02-01 00:00:00
abstract::Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-07
更新日期:2008-01-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) envelope glycoprotein D (gD) plays an essential role in viral entry. The functional regions of gD responsible for viral entry have been mapped to its extracellular domain, whereas the gD cytoplasmic domain plays no obvious role in viral entry. Thus far, the role(s) of the gD cytoplasmic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01396-16
更新日期:2016-10-28 00:00:00
abstract::The St-1 genome is about 6,050 base pairs in size, approximately 10% larger than phiX174 (5,375 base pairs). The DNA fragments obtained by HincII, HaeIII, and EcoRI digestion were ordered and aligned into a colinear map, and the single BglI cleavage site was located. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.738-744.1978
更新日期:1978-09-01 00:00:00
abstract::GLUT1 has recently been suggested to be a binding receptor for human T-cell leukemia virus type 1 (HTLV-1). We used a novel, short-term assay to define the role of GLUT1 in cell-to-cell transmission. Although increasing cell surface levels of GLUT1 enhanced HTLV-I transfer, efficient virus spread correlated largely wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01522-06
更新日期:2007-02-01 00:00:00
abstract::The fundamental event in prion disease is thought to be the posttranslational conversion of the cellular prion protein (PrPC) into a pathogenic isoform (PrPSc). The occurrence of PrPC on the cell surface and PrPSc in amyloid plaques in situ or in aggregates following purification complicates the study of the molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9413-9418.1998
更新日期:1998-11-01 00:00:00
abstract::Nondefective reticuloendotheliosis virus induces chicken bursal lymphoma in a manner similar to that of avian leukosis virus. The provirus integrates in the c-myc locus and uses a promoter insertion mechanism to activate c-myc expression. We cloned a provirus involved in c-myc activation from a B lymphoma. Detailed st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2084-2090.1987
更新日期:1987-07-01 00:00:00
abstract::The effect of 5-bromodeoxyuridine (BrdU) on vaccinia virus-induced polypeptide synthesis in BSC-1 cells has been investigated. Most virus-induced pre- and post-replicative polypeptides were synthesized in concentrations of 5-bromodeoxyuridine that inhibited virus growth. The synthesis of a few post-replicative polypep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1131-1133.1976
更新日期:1976-06-01 00:00:00
abstract::Recombinant vaccinia viruses that express defective retroviral vectors upon a single infection event in normal host cells were constructed. The gag-pol and envelope genes and a retroviral vector unit were inserted as vaccinia virus promoter-controlled transcription units at three separate loci. The triple recombinant ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.7017-7025.2003
更新日期:2003-06-01 00:00:00
abstract::The adaptive immune system generates CD8 cytotoxic T lymphocytes (CTLs) as a major component of the protective response against viruses. Knowledge regarding the nature of the peptide sequences presented by HLA class I molecules and recognized by CTLs is thus important for understanding host-pathogen interactions. In t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13399-13408.2004
更新日期:2004-12-01 00:00:00
abstract::A region in addition to and outside the long terminal repeats (LTRs) in the gag gene of the Prague A strain of Rous sarcoma virus was found to be essential in cis for efficient cell transformation by cloned viral DNA. Transformation in chicken embryo fibroblasts, which requires infectious virus production and reinfect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3401-3409.1987
更新日期:1987-11-01 00:00:00
abstract::Endogenous retroviruses (ERV) are found throughout vertebrate genomes, and failure to silence their activation can have deleterious consequences on the host. Mutation and subsequent disruption of ERV loci is therefore an indispensable component of the cell-intrinsic defenses that maintain the integrity of the host gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00728-19
更新日期:2019-09-30 00:00:00
abstract::A cloned segment of the polyoma virus genome encoding the small T antigen has been fused, in the correct phase for translation, to the 5' end of the beta-galactosidase gene. The hybrid gene, cloned in Escherichia coli, produces a protein resembling the small T antigen. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.125-132.1980
更新日期:1980-10-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infects alveolar macrophages (AMϕ), causing dysregulated alpha interferon (IFN-α) and tumor necrosis factor alpha (TNF-α) production through a mechanism(s) yet to be resolved. Here, we show that AMϕ infected with PRRSV secreted a reduced quantity of IFN-α fol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01251-17
更新日期:2018-01-02 00:00:00
abstract::The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4206-4212.1995
更新日期:1995-07-01 00:00:00
abstract::Translation of early RNA specific for the leftmost early region 1 of adenovirus type 5 DNA in a rabbit reticulocyte cell-free system resulted in the synthesis of proteins with the following molecular weights: 65,000 (65K), 54K, 42 to 34K, 29K, 25K, 19K, and 18K. All of these proteins could be immunoprecipitated with h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.524-529.1981
更新日期:1981-01-01 00:00:00
abstract::Experimental reverse genetic replacement of Epstein-Barr virus nuclear antigen 3A (EBNA3A) with a conditional mutant EBNA3A revealed that EBNA3A is critical for continued lymphoblastoid cell (LCL) growth. Wild-type (wt) EBNA3A expressed in the LCLs specifically sustained growth under nonpermissive conditions, whereas ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10171-10179.2005
更新日期:2005-08-01 00:00:00
abstract::Rous sarcoma virus protein p10 is a gag component of the virion present in stoichiometric amount but of unknown function. To characterize this protein, a series of mutants of p10 with linker insertions or deletions was generated by site-directed mutagenesis of a cloned proviral DNA. The deletions and two of the linker...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3826-3834.1993
更新日期:1993-07-01 00:00:00
abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
abstract::We have developed idiotype-anti-idiotype monoclonal antibodies that provide evidence for rabies virus binding to the acetylcholine receptor (AChR). Hybridoma cell lines 7.12 and 7.25 resulted after fusion of NS-1 myeloma cells with spleen cells from a BALB/c mouse immunized with rabies virus strain CVS. Antibody 7.12 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.530-542.1993
更新日期:1993-01-01 00:00:00
abstract::Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a w...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/JVI.00367-11
更新日期:2011-07-01 00:00:00
abstract::In this study, we investigated the interaction of reovirus particles with cell membranes by using a 51Cr release assay. We confirmed prior observations (J. Borsa, B. D. Morash, M. D. Sargent, T. P. Copps, P. A. Lievaart, and J. G. Szekely, J. Gen. Virol. 45:161-170, 1979) that intermediate subviral particles (ISVPs) o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5339-5345.1993
更新日期:1993-09-01 00:00:00
abstract::The P4 promoter of the parvovirus minute virus of mice contains a single degenerate GC box sequence which binds the transcription factor Sp1 with high affinity. The two promoters of murine Sp1 were affinity purified, and their interactions with the P4 promoter were examined. Several unusual features were observed. Met...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6661-6670.1991
更新日期:1991-12-01 00:00:00