Abstract:
:We aimed to identify a novel flavonoid from the in-house natural products to suppress matrix metalloproteases (MMPs), which is responsible for degradation of collagen and other extracellular matrix proteins. Total eight natural products were screened for identification of a novel MMP-9 suppressor using MMP-9 reporter system, where the prompt initial screening with multiple samples is readily examined. Among the extracts used in the present study, one extract (Citrus unshiu) was found active in this assay system. Furthermore, three representative flavonoids in this active extract of Citrus unshiu peel were tested in MMP-9 reporter system. Nobiletin (NB) of the tested flavonoids suppressed MMP-9 expression without cytotoxicity, which was validated by both real-time polymerase chain reaction (PCR) and zymography analyses. Sustained p38 mitogen activated protein kinase (MAPK) activity, closely associated with induction of MMP-9 under stress condition, was markedly reduced by NB treatment, which implies that modulation of p38MAPK by nobiletin is responsible for reduction of MMP9 expression. Hence, nobiletin, identified from MMP-9 reporter system based screening, may be further applied for the purpose of delaying collagen degradation in skin fibroblasts.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Kim JJ,Korm S,Kim WS,Kim OS,Lee JS,Min HG,Chin YW,Cha HJdoi
10.1248/bpb.b13-00534subject
Has Abstractpub_date
2014-01-01 00:00:00pages
158-63issue
1eissn
0918-6158issn
1347-5215pii
DN/JST.JSTAGE/bpb/b13-00534journal_volume
37pub_type
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