Standardization of a method to study angiogenesis in a mouse model.

Abstract:

:In the adult organism, angiogenesis is restricted to a few physiological conditions. On the other hand, uncontrolled angiogenesis have often been associated to angiogenesis-dependent pathologies. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. This work aimed to standardize a method for the study of angiogenesis and to investigate the effects of thalidomide on angiogenesis. Sponges of 0.5 x 0.5 x 0.5 cm were implanted in the back of mice groups, control and experimental (thalidomide 200 mg/K/day by gavage). After seven days, the sponges were removed. The dosage of hemoglobin in sponge and in circulation was performed and the ratio between the values was tested using nonparametric Mann-Whitney test. Results have shown that sponge-induced angiogenesis quantitated by ratio between hemoglobin content in serum and in sponge is a helpful model for in vivo studies on angiogenesis. Moreover, it was observed that sponge-induced angiogenesis can be suppressed by thalidomide, corroborating to the validity of the standardized method.

journal_name

An Acad Bras Cienc

authors

Feder D,Perrazo FF,Pereira EC,Forsait S,Feder CK,Junqueira PE,Junqueira VB,Azzalis LA,Fonseca FL

doi

10.1590/0001-3765201378011

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

1483-7

issue

4

eissn

0001-3765

issn

1678-2690

pii

S0001-37652013000401483

journal_volume

85

pub_type

杂志文章
  • Population structure and allometry of Podocnemis unifilis (Testudines, Podocnemididae) in a protected area upstream Belo Monte dam in Xingu River, Brazil.

    abstract::Amazon river turtles are increasingly threatened by habitat loss and alteration due to the Brazilian energy policy based on construction of hydroelectric dams, meanwhile, populational studies remain scarce. We described the population structure, and established body allometric relationships of Podocnemis unifilis in t...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201520140321

    authors: Miorando PS,Giarrizzo T,Pezzuti JC

    更新日期:2015-10-01 00:00:00

  • Physicochemical quality profiles of commercial oral tablets and capsules containing lutein - impact of insufficient specific sanitary regulations.

    abstract::Dietary supplements in many countries such as the USA do not require registration prior to commercialization. The Agência Nacional de Vigilância Sanitária (ANVISA) registers substances with functional properties as foods. Lutein is a carotenoid with antioxidant activity available on the market. However, no regulatory ...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201820170972

    authors: Anselmo CS,Mendes TC,Cabral LM,Sousa VP

    更新日期:2018-07-01 00:00:00

  • Modeling the reflection of Photosynthetically active radiation in a monodominant floodable forest in the Pantanal of Mato Grosso State using multivariate statistics and neural networks.

    abstract::The study of radiation entrance and exit dynamics and energy consumption in a system is important for understanding the environmental processes that rule the biosphere-atmosphere interactions of all ecosystems. This study provides an analysis of the interaction of energy in the form of photosynthetically active radiat...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201620150176

    authors: Curado LF,Musis CR,Cunha CR,Rodrigues TR,Pereira VM,Nogueira JS,Sanches L

    更新日期:2016-09-01 00:00:00

  • Litter Size Reduction Induces Metabolic and Histological Adjustments in Dams throughout Lactation with Early Effects on Offspring.

    abstract::In the present study we analyzed morphological and metabolic alterations in dams nursing small litters and their consequences to offspring throughout lactation. Offspring sizes were adjusted to Small Litter (SL, 3 pups/ dam) and Normal Litter (NL, 9 pups/ dam). Body weight, food intake, white adipose tissue (WAT) cont...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201920170971

    authors: Xavier JLP,Scomparin DX,Pontes CC,Ribeiro PR,Cordeiro MM,Marcondes JA,Mendonça FO,Silva MTD,Oliveira FB,Franco GCN,Grassiolli S

    更新日期:2019-03-21 00:00:00

  • Culicoides castelloni sp. nov. from the Brazilian Amazon Region with a revision of the reticulatus species group (Diptera, Ceratopogonidae).

    abstract::A new species of the reticulatus species group, C. castelloni Santarém and Felippe-Bauer, is described and illustrated based on female specimens from the state of Amazonas, Brazil. A systematic key, wing photographs and table with numerical characters of females and a synopsis of 24 species of the Culicoides reticulat...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201520140517

    authors: Santarém MC,Farias ES,Felippe-Bauer ML

    更新日期:2015-04-01 00:00:00

  • New azhdarchoid pterosaur (Pterosauria, Pterodactyloidea) with an unusual lower jaw from the Portezuelo Formation (Upper Cretaceous), Neuquén Group, Patagonia, Argentina.

    abstract::A new azhdarchoid pterosaur from the Upper Cretaceous of Patagonia is described. The material consists of an incomplete edentulous lower jaw that was collected from the upper portion of the Portezuelo Formation (Turonian-Early Coniacian) at the Futalognko site, northwest of Neuquén city, Argentina. The overall morphol...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720170478

    authors: Kellner AWA,Calvo JO

    更新日期:2017-01-01 00:00:00

  • Agaricus subrufescens: substratum nitrogen concentration and mycelial extraction method on antitumor activity.

    abstract::Antitumor activity of Agaricus subrufescens has been shown on vegetative mycelium and basidiocarp. However, few studies have assessed the effect of A. subrufescens cultivation conditions and extraction methods on antitumor activity. This study evaluated the effect of nitrogen concentration on the cultivation medium of...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201620160161

    authors: Bertéli MB,Lopes AD,Colla IM,Linde GA,Colauto NB

    更新日期:2016-10-01 00:00:00

  • Direct determination of sodium and potassium in blood serum by flow injection and atomic absorption spectrophotometry.

    abstract::A simple and reliable method for the measurement of sodium and potassium in blood serum without any sample dilution by using flow injection and atomic absorption spectrophotometry is described. A sample throughout of 100 measurements per hour is possible. Matrix problems have not been encountered. The coefficient of v...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:

    authors: Burguera JL,Burguera M,Gallignani M

    更新日期:1983-06-01 00:00:00

  • Secondary structure of soybean trypsin inhibitor (Kunitz): a study by infrared spectroscopy.

    abstract::The infrared spectrum (amide I' region) of Kunitz soybean trypsin inhibitor (SBTI) was obtained in D2O solution and resolved into Gaussian components. A prominent broad band centered at 1643 cm-1 is shown on the unresolved spectrum, which is usually assigned to N-deuterated peptide groups in an unordered structure, si...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:

    authors: Ventura MM

    更新日期:1989-01-01 00:00:00

  • Paleoenvironmental Evolution of Continental Carbonates in West-Central Brazil.

    abstract::This paper presents a sedimentological and stratigraphical study of Quaternary (Middle to Late Pleistocene/Holocene) continental carbonates outcrops inside Pantanal Basin and its surroundings, especially in Serra da Bodoquena, Pantanal do Miranda and Corumbá/Ladário plateau, in the state of Mato Grosso do Sul, as well...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720160584

    authors: Oliveira EC,Rossetti DF,Utida G

    更新日期:2017-05-01 00:00:00

  • Synthesis of new N-alkyl-2-mercapto-1-octylamines and respective disulfides.

    abstract::Four new N-alkyl-2-mercapto-1-octylamine hydrochlorides were synthesized via the reaction of 1,2-epithio-octane with corresponding amines following by conversion to the hydrochlorides. The disulfides were obtained by oxidation of the respective thiols. Upon biological assay of the products for activity against infecti...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:

    authors: Vieira Filho SA,Nelson DL,Piló-Veloso D

    更新日期:1986-12-01 00:00:00

  • Growth and reproduction of the mangrove crab Goniopsis cruentata (Latreille, 1803) (Crustacea: Decapoda: Grapsidae) in southeastern Brazil.

    abstract::Goniopsis cruentata is a common semi-terrestrial crab in Brazilian mangroves and an important fishery resource for traditional communities in the northeastern Brazilian coast. Aiming to contribute to the knowledge about the species, this study evaluated the carapace width and weight growth curves, the relative growth ...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201520130387

    authors: Reis CR,Taddei FG,Cobo VJ

    更新日期:2015-04-01 00:00:00

  • Some considerations about the ecology of Triatominae.

    abstract::Observations on the ecology of silvatic Triatominae are still fragmentary, principally because target species in control process are almost exclusively domestic. Report of silvatic species invading human dwellings must lead research to be focused on their original ecology, in order to assist in understanding their pro...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/s0001-37652005000300006

    authors: Noireau F,Carbajal-de-la-Fuente AL,Lopes CM,Diotaiuti L

    更新日期:2005-09-01 00:00:00

  • Sodium reduction in margarine using NaCl substitutes.

    abstract::Sodium chloride is traditionally used as a food additive in food processing. However, because of its high sodium content, NaCl has been associated with chronic diseases. Margarine is a popular product that is used in several preparations, but it includes high sodium content; therefore, it is among the products whose s...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720150618

    authors: Gonçalves C,Rodrigues J,Júnior H,Carneiro J,Freire T,Freire L

    更新日期:2017-01-01 00:00:00

  • The evidence for hippocampal long-term potentiation as a basis of memory for simple tasks.

    abstract::Long-term potentiation (LTP) is the enhancement of postsynaptic responses for hours, days or weeks following the brief repetitive afferent stimulation of presynaptic afferents. It has been proposed many times over the last 30 years to be the basis of long-term memory. Several recent findings finally supported this hyp...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/s0001-37652008000100007

    authors: Izquierdo I,Cammarota M,Da Silva WC,Bevilaqua LR,Rossato JI,Bonini JS,Mello P,Benetti F,Costa JC,Medina JH

    更新日期:2008-03-01 00:00:00

  • Prevalence and Fluconazole Susceptibility Profile of Candida spp. Clinical Isolates in a Brazilian Tertiary Hospital in Minas Gerais, Brazil.

    abstract::Candidiasis has become an important concern for clinical practice, especially with the increasing incidence of immunocompromised patients. In this scenario, the development resistance to fluconazole presents a challenge for treating these opportunistic infections. The aim of this study was to evaluate some epidemiolog...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201520140717

    authors: Neves-Junior A,Cartágenes-Pinto AC,Rocha DA,de Sá LF,Junqueira Mde L,Ferreira-Pereira A

    更新日期:2015-08-01 00:00:00

  • Asymmetric bioreduction of β-ketoesters derivatives by Kluyveromyces marxianus: influence of molecular structure on the conversion and enantiomeric excess.

    abstract::This study presents the bioreduction of six β-ketoesters by whole cells of Kluyveromyces marxianus and molecular investigation of a series of 13 β-ketoesters by hologram quantitative structure-activity relationship (HQSAR) in order to relate with conversion and enantiomeric excess of β-stereogenic-hydroxyesters obtain...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720170118

    authors: Oliveira SSS,Bello ML,Rodrigues CR,Azevedo PL,Ramos MCKV,Aquino-Neto FR,Fiaux SB,Dias LRS

    更新日期:2017-07-01 00:00:00

  • Trade-off between reservoir yield and evaporation losses as a function of lake morphology in semi-arid Brazil.

    abstract::This study investigates the relationships between yield and evaporation as a function of lake morphology in semi-arid Brazil. First, a new methodology was proposed to classify the morphology of 40 reservoirs in the Ceará State, with storage capacities ranging from approximately 5 to 4500 hm3. Then, Monte Carlo simulat...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201620150124

    authors: Campos JN,Lima IE Neto,Studart TM,Nascimento LS

    更新日期:2016-05-31 00:00:00

  • Assessment of the yield loss imparted by Orobanche aegyptiaca in tomato in Pakistan.

    abstract::Broomrapes (Orobanche sp.) are root holoparasitic plants causing severe damage to various families of horticultural crops. A Preliminary survey was carried in tomato fields infested with orobanche in Potohar plateau of Pakistan. Results indicated that each planting bed comprising10-12 tomato plants contained 7-8 paras...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201820180098

    authors: Ahmad T,Ahmad B,Tariq RMS,Syed MZ,Ahmad Z

    更新日期:2018-10-01 00:00:00

  • Data Science Strategies for Multimessenger Astronomy.

    abstract::This article aims to identify and suggest data science strategies to strengthen scientific research in astronomy. The improvements in data workflow performance that can be provided by these strategies can be crucial to the multimessenger astronomy (MMA). A special focus is given to the treatment of raw data in the con...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765202020200861

    authors: Rosa RR

    更新日期:2020-09-28 00:00:00

  • Notes on court and copula, fertility, nest, eggs and hatchlings of the Caatinga's black snake Boiruna sertaneja Zaher, 1996 (Serpentes: Dipsadidae) from northeastern Brazil.

    abstract::The Caatinga's black snake Boiruna sertaneja is a Pseudoboini species, endemic of the Caatinga biome. It is rare and fits in five traits that suggest it deserve more attention in reproduction research and conservation policies. Here we provide information on reproductive biology of B. sertaneja by adding new data abou...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765202020190588

    authors: Guedes T,Guedes A

    更新日期:2020-01-01 00:00:00

  • Diversity and phenology of epigeal Coleoptera assemblages in lettuce and tomato crops in Northern Buenos Aires province, Argentina.

    abstract::Epigeic coleopteran species are linked by complex trophic webs and implicated in several ecosystem services for agriculture. Although there are reports of ground-dwelling Coleopterans inhabiting main extensive agroecosystems, their assemblages in horticultural crops in Argentina have been little explored. We examined ...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765202020181391

    authors: Rouaux J,Cabrera N,MartÍnez AS,Posse MC,Luna MG

    更新日期:2020-01-01 00:00:00

  • The Evolution of Pneumatic Foramina in Pterosaur Vertebrae.

    abstract::Pterosaurs possess skeletal pneumatization, which can be assessed externally through the observation of pneumatic foramina. These foramina vary in position, shape, and number among taxa. Here we propose new characters referring to the position and number of pneumatic foramina present in cervical and dorsal vertebrae o...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201920180782

    authors: Buchmann R,Rodrigues T

    更新日期:2019-01-01 00:00:00

  • The protective effects of β-sitosterol and vermicularin from Thamnolia vermicularis (Sw.) Ach. against skin aging in vitro.

    abstract::Aged skin, featured with dryness and wrinkles, has received mounting attention due to its adverse influences on beauty. β-Sitosterol and vermicularin are two common active ingredients of Thamnolia vermicularis (Sw.) Ach., a traditional Chinese medicine, of which the anti-aging effect has been discovered. Their protect...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201920181088

    authors: Haiyuan YU,Shen X,Liu D,Hong M,Lu Y

    更新日期:2019-12-02 00:00:00

  • Beyond diversity loss and climate change: Impacts of Amazon deforestation on infectious diseases and public health.

    abstract::Amazonian biodiversity is increasingly threatened due to the weakening of policies for combating deforestation, especially in Brazil. Loss of animal and plant species, many not yet known to science, is just one among many negative consequences of Amazon deforestation. Deforestation affects indigenous communities, rive...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章,评审

    doi:10.1590/0001-3765202020191375

    authors: Ellwanger JH,Kulmann-Leal B,Kaminski VL,Valverde-Villegas JM,Veiga ABGD,Spilki FR,Fearnside PM,Caesar L,Giatti LL,Wallau GL,Almeida SEM,Borba MR,Hora VPD,Chies JAB

    更新日期:2020-04-17 00:00:00

  • Physical and chemical study of lattice kaolinites and their interaction with orthophosphate.

    abstract::This study covers the evaluation of the structure and the capacity in adsorbing orthophosphate ions of kaolinites collected from sampling sites in Manaus (Brazil). The kaolinites were obtained using physical fractioning (sieving/siphoning) techniques and chemical treatment with HCl, H2SO4, H2O2 and KCl. The investigat...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720160519

    authors: Silva MSE,Lages AS,Santana GP

    更新日期:2017-07-01 00:00:00

  • Evaluation of the impact of pharmaceutical care for tuberculosis patients in a Secondary Referral Outpatient Clinic, Minas Gerais, Brazil.

    abstract::Pharmaceutical care is a professional practice seeking the responsible provision of drug therapy by identifying, resolving, and preventing Drug-Related Problems (DRP). The study aims to describe and evaluate the impact of pharmaceutical care given to patients being treated for tuberculosis (TB). Study concurrent, long...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720170301

    authors: Lopes ARV,Miranda SS,Ceccato MDGB,Silveira MR,Resende NH,Carvalho WS

    更新日期:2017-10-01 00:00:00

  • Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities.

    abstract::Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproli...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201720160725

    authors: Santos TLAD,Queiroz RF,Sawaya ACHF,Lopez BG,Soares MBP,Bezerra DP,Rodrigues ACBC,Paula VF,Waldschmidt AM

    更新日期:2017-01-01 00:00:00

  • The Brazilian time and frequency atomic standards program.

    abstract::Cesium atomic beam clocks have been the workhorse for many demanding applications in science and technology for the past four decades. Tests of the fundamental laws of physics and the search for minute changes in fundamental constants, the synchronization of telecommunication networks, and realization of the satellite...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/s0001-37652008000200002

    authors: Ahmed M,Magalhães DV,Bebeachibuli A,Müller ST,Alves RF,Ortega TA,Weiner J,Bagnato VS

    更新日期:2008-06-01 00:00:00

  • Osteohistology of the silesaurid Sacisaurus agudoensis from southern Brazil (Late Triassic) and implications for growth in early dinosaurs.

    abstract::The non-dinosaurian dinosauriform silesaurids are the closest relatives of crown-group dinosaurs and are thus, important for understanding the origins of that group. Here, we describe the limb bone histology of the Late Triassic silesaurid Sacisaurus agudoensis from the Candelária Sequence of the Santa Maria Supersequ...

    journal_title:Anais da Academia Brasileira de Ciencias

    pub_type: 杂志文章

    doi:10.1590/0001-3765201920180643

    authors: Veiga FH,Botha-Brink J,Ribeiro AM,Ferigolo J,Soares MB

    更新日期:2019-06-19 00:00:00