Abstract：
:Increased lipid peroxidation (LP) occurs in the presence of various transition, metal ions, including copper and iron, as well as various heme moieties. In addition, irradiation significantly increases LP. The degree of LP, as determined by the liquid chromatographic measurement of plasma malondialdehyde (MDA), can be significantly reduced by the addition of various metal chelating agents and other antioxidants to both stored red blood cells and platelet concentrates. They are also effective in reducing the degree of LP following irradiation. In the current study, leukocytes were effectively removed by filtration from platelet concentrates prepared by standard methods (mean +/- SD before filtration, 460 +/- 286 per microL vs 0.3 +/- 0.2 per microL after filtration). The removal of the white blood cells significantly reduced the plasma MDA levels in the leukocyte-free platelet concentrates compared with concentrates containing leukocytes after storage for 4, 7, and 11 days (P < 0.001; P < 0.02; P < 0.05, respectively). In addition, plasma potassium levels were also significantly reduced in the leukocyte-free platelet concentrates (P < 0.001). Our results suggest that the early removal of leukocytes from platelet concentrates may be helpful in prolonging platelet viability and longevity.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Knight JA,Searles DA,Blaylock RCsubject
Has Abstractpub_date
1994-01-01 00:00:00pages
69-75issue
1eissn
0091-7370issn
1550-8080journal_volume
24pub_type
杂志文章abstract：:In vitro determination of allergen-specific IgE (sIgE) represents an important aid in the diagnosis and treatment of allergy. Improvements in laboratory methodology--the development of second, and now third generation assays for sIgE--have brought about major advances in speed, convenience, performance, and in the sta...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:We examined the results of 1.3 million drug tests performed on patients being monitored for compliance with pain medications and substance abuse rehabilitation to determine if the 2016 CDC prescribing guidelines had any impact on opiate benzodiazepine use. We observed that the combination of the opiate drugs morphine,...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2020-05-01 00:00:00
abstract：:The radiation modifying effect and toxicity of tetraethylthiuram disulfide (disulfiram) have been studied. Disulfiram (DSM) inhibits aldehyde dehydrogenase, dopamine-beta-oxygenase, microsomal mixed-function oxidases and cytochrome P-450 enzymes. It is widely used for aversion therapy in alcoholism. Disulfiram also in...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:Pancreatic cancer is one of the most lethal diseases among solid malignancies. Most patients are diagnosed in the late stage or after metastasis. Notably, pancreatic cancer is resistant to chemotherapy and lacks efficient target therapy methods. Given that, investigating the mechanism of metastasis, finding biomarkers...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2019-05-01 00:00:00
abstract：:The objectives of this prospective, cross-sectional study were to characterize blood donors in an academic-based hospital donor center, to determine whether physicians donate, and to elucidate the donation impetus. A confidential survey was issued to presenting, potential donors over 200 weekdays. Three questions were...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：:Previous studies have suggested that there is a disproportionally higher risk of infection following traumatic brain injury (TBI). This predisposition to infection may be driven by a poorly understood, brain-specific response in the immune system after TBI. However, there is a lack of studies that have fully character...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：:Human amniotic-derived cells (hAMCs) have recently raised interest for their differentiation capability and immunomodulatory properties. To assess the feasibility of hAMCs therapeutic treatment during fetal development, we explored the localization of cells derived from the human amniotic membrane in rat organs after ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：:The existence of clonorchiasis cases among the Asian immigrants and the major clinical and pathologic features encountered in those patients is emphasized. Owing to the longevity of the parasite, clinical symptoms may occur long after endemic exposure. The common manifestations among the immigrants are recurrent pyoge...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1984-05-01 00:00:00
abstract：BACKGROUND:Various assays for detecting high-risk human papillomavirus (HR HPV) have been introduced recently, including the Abbott RealTime High-Risk HPV assay. We sought to compare the performance of Abbott PCR to Hybrid Capture 2 for the detection of HR HPV. METHODS:A total of 941 cervical swab specimens were obtai...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：:In the course of a clinical comparison involving 204 parallel total creatine kinase (CK), creatine kinase-MB isoenzyme (CK-MB), and cardiac troponin I (cTnI) measurements, 12 patients were identified in whom cTnI was elevated while total CK was normal, as well as 2 patients in whom CK-MB was elevated while cTnI was no...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:The role of the mammalian mesonephric kidney is not completely understood. It has been established that outpouchings of the mesonephric excretory ducts give origin to parts of the urogenital system of the adult. It is also known that mammalian mesonephric urine is formed as an ultrafiltrate. The mesonephric renal tubu...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:To assess mitochondrial function (pyruvate dehydrogenase [PDH] activity), cells were grown in the appropriate media to confluence, rinsed and incubated in glucose free media containing 25 microM L-lactate and [1-14C]-D,L-lactate. Lactate oxidation was measured as the amount of lactate oxidized in nmol of 14CO2 generat...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：BACKGROUND:Carbohydrate antigen series biomarkers in cerebrospinal fluid (CSF) are important for the diagnosis of brain metastasis and meningeal carcinomatosis. Its relationship with CSF and serum in non-neoplastic diseases may be beneficial for earlier diagnosis and treatment. MATERIALS AND METHODS:161 pairs of CSF a...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:MicroRNAs (miRNAs) are associated with hepatocellular carcinoma (HCC) progression and metastasis. However, it is unclear whether they could act as biomarkers for HCC diagnosis and prognosis. METHODS:In this study, the miR-122 and miR-199a expression levels were determined by quantitative real-time PCR (qRT-P...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2020-03-01 00:00:00
abstract：:Degenerative joint disease (DJD) is characterized by pain on use. X-rays show cartilage narrowing and osteophytes. Synovial effusions are non-inflammatory, i.e. clear wiht good viscosity and less than 2000 WBC per mm. 3 Cartilage fragments may be seen in the joint fluid. Important systemic diseases that can cause dege...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydr...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:The aim of this study was to assess the efficacy and pharmacokinetic profiles of gentamicin, vancomycin, and levofloxacin in a rat air pouch model, in which Staphylococcus aureus (ATCC 25293) was used as the test organism. Antibiotic treatments (i.p.) were started 1 hour after bacterial inoculation and continued for 5...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The pyrrolizidine alkaloid monocrotaline produces pulmonary inflammation, hemorrhage, fibrosis, and hypertension. In rats, monocrotaline pneumotoxicity can be ameliorated by cotreatment with inhibitors of angiotensin converting enzyme (ACE), such as CL242817. In the present study, serum and urine copper (Cu) concentra...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Trehalose 6,6' dimycolate (TDM), a mycobacterial glycolipid, induces granulomas and hemorrhagic toxic reactions when administered in oil but not as a suspension in saline. It was previously demonstrated by us that TDM forms highly structured layers at oil-water interfaces and then postulated that its toxicity derives ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:This paper is a summary of studies over the past few years that pertain to animal neurotoxins. These toxins are found throughout the animal kingdom. Homologies exist in the structures of these poisons within classes and point to conservation of active sites throughout evolution. In the case of the peptides, invariant ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:In vitro activity of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) does not always correlate with in vivo hemolytic manifestations. Many of these non-correlations are reviewed and can now be explained on the basis of altered substrate affinity and/or altered inhibition by intracellular metabolites. These alte...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:A total of 979 cardiac profiles were reviewed. Seventeen cases were found to have elevated CK-BB by electrophoresis and were misidentified by the immunoinhibition/immunoprecipitation technique as elevated creatine phosphokinase (CK-MB). Eleven of the 17 cases also had elevated lactate dehydrogenase (LD) LD-5/LD-1 rati...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:This study evaluated the performance of a handheld coagulation analyzer for measurements of capillary blood specimens of 93 outpatient cardiology patients with atrial fibrillation who were receiving oral anti-coagulant therapy. The international normalized ratio (INR) results of the CoaguChek XS system (Roche Diagnost...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Prospective HLA-DR matching has been shown to enhance renal graft survival. It has also been demonstrated that the major stimuli in the one-way mixed lymphocyte culture reaction (MLR) are the DR rather than DQ class II histocompatibility antigens. A two antigen DR match between the potential recipient and donor is usu...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:A case of multiple myeloma producing IgD-M component with light chains of lambda type is reported. It involved the skeleton and was complicated by compression of the spinal cord and massive extension to the pleura. The urine contained lambda Bence Jones protein. Under the electron microscope, the neoplastic plasma cel...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:Renal arterioles from five patients with benign essential hypertension (BH) and four patients with malignant essential hypertension (MH) were studied using electron microscopy (EM). This study suggests a concept of different pathogenetic mechanism for renal vascular (arteriolar) lesions in BH and MH (i.e., benign and ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Chronic progressive external ophthalmoplegia (CPEO) is a mitochondrial myopathy commonly caused by deleterious changes in the mitochondrial DNA (mtDNA). We describe a 45-year-old man who was referred to us for investigation of progressive ptosis. We performed a multiplex ligation-dependent probe amplification (MLPA) a...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：:To define further the nature of the decreased responsiveness of neonatal neutrophils to chemotactic factor stimulation, neutrophil shape change induced by various concentrations of N-formyl-methionyl-leucyl-phenyl-alanine (fMLP) was studied using a whole blood assay. Samples from 48 full term neonates and paired healt...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:An in-house method was used, including dissociation of Somatomedin C/insulin-like Growth Factor I (Sm-C/IGF-I) binding proteins with acid and extraction of Sm-C/IGF-I by C-2 cartridge before radioimmunoassay, to measure plasma Sm-C/IGF-I levels in three groups of patients--total of 19--with acromegaly who underwent tr...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Iron accumulation, especially that of free oxidized ferrous iron, has been shown to induce tissue oxidative damage and contribute to brain aging and the development of neurodegenerative disease. Here we examine whether sex and advanced age affect the expression of iron-related molecules that participate in regulating ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
