Abstract：
:To assess mitochondrial function (pyruvate dehydrogenase [PDH] activity), cells were grown in the appropriate media to confluence, rinsed and incubated in glucose free media containing 25 microM L-lactate and [1-14C]-D,L-lactate. Lactate oxidation was measured as the amount of lactate oxidized in nmol of 14CO2 generated per mg of protein per minute. Basal activity varied with cell number and the cell type studied: fibroblast 2.26 +/- 0.01; Chinese hamster ovary (CHO) 42 +/- 0.4; BC3H-1 52 +/- 2.1 nmol per mg per minute. The CHO cells screened for PDH activity decreased their dependence on lactate as a substrate in the presence of 5mM glucose by 60 percent. Increasing the cold lactate concentration diluted the labelled lactate available for pyruvate oxidation in a dose dependent manner. The mitochondrial inhibitor rotenone (25 microM) decreased assay activity by > 75 percent in CHO and BC3H-1 cells. The lactate oxidation assay was shown to be sensitive enough to measure insulin stimulation of PDH in a dose dependent manner with maximum activity occurring at concentrations between 1 microU per ml and 100 microU per ml.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Ofenstein JP,Dandurand DM,Kiechle FLsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
406-13issue
6eissn
0091-7370issn
1550-8080journal_volume
22pub_type
杂志文章abstract：BACKGROUND:We compared the AdvanSure hepatitis B virus real-time polymerase chain reaction (AdvanSure HBV) kit with three other HBV DNA quantification assays and evaluated its performance. METHODS:The AdvanSure HBV real-time PCR assay was compared with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV T...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:Clostridium difficile is one of the most common causes of nosocomial diarrhea, and diagnostic methods for detecting C. difficile infection have shifted from conventional to more recent molecular techniques. This study aimed to compare the performance of two molecular assays (Meridian Illumigene™ and AdvanSur...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Secretion of nickel into unstimulated and stimulated saliva of rabbits was studied after a single intraperitoneal injection of nickel chloride labelled with 63Ni. Maximum 63Ni in unstimulated saliva was obtained in specimens collected one hr after maximum serum-63Ni was reached and two hrs after the nickel administrat...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Patients with diabetes mellitus have an increased risk of thrombosis and accelerated atherogenesis. Increased platelet adhesion and aggregation are noted in vitro. This paper reviews known platelet abnormalities found in patients with diabetes mellitus (DM) and examines the pathophysiology associated with these abnorm...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
abstract：:The ability of three isolates of serogroup 1 and one isolate of serogroup 4 of Legionnaires' disease bacterium (LDB) to infect and cause fever and death in guinea pigs was studied, as well as their ability to produce plaques in cultured primary chick embryo cells. The serogroup 4 isolate originally was recovered from ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Oxygen toxicity was first described in laboratory animals in 1878 and was further established in 1899. The first experiment regarding a free radical reaction was reported in 1894. However, it was not until the late 1940s to early 1950s that retrolental fibroplasia in premature newborns was recognized as being due to o...
journal_title：Annals of clinical and laboratory science
pub_type： 历史文章,杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
abstract：:The aging process in man leads to some loss of kidney mass and function. The kidney produces epidermal growth factor (EGF), a polypeptide involved in the repairing process of epithelial cells. Human urine contains high concentrations of EGF, derived from its production in the kidney. It is not known if aging alters ur...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:The transmembrane protease, serine 2-E twenty-six related gene (TMPRSS2-ERG) fusion leading to ERG overexpression, was detected in approximately 50% of prostate cancers (ranging from 35-70%). However, the published data on ERG expression in tumors from other organs and normal tissues were limited. In this study, we in...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Adherence of 14C-glucose labeled Candida albicans to vaginal epithelial cells was measured in the presence of 10 potential inhibitory sugars at concentrations of 5 to 150 mg per ml for 90 minutes at 37 degrees C. In competitive inhibition experiments, no inhibition of adherence was seen with any sugar at any concentra...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Degenerative joint disease (DJD) is characterized by pain on use. X-rays show cartilage narrowing and osteophytes. Synovial effusions are non-inflammatory, i.e. clear wiht good viscosity and less than 2000 WBC per mm. 3 Cartilage fragments may be seen in the joint fluid. Important systemic diseases that can cause dege...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：CONTEXT:Hepatitis C virus (HCV) infects up to 1.8% of the US general population, although the rate is significantly higher in military veterans at 5.4-20%. Early detection and accurate diagnosis are critical as chronic HCV infection can lead to liver cirrhosis and hepatocellular carcinoma. Genotype analysis has both th...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：:To investigate the relationships between obesity and serum lipid concentrations, we measured eight anthropometric parameters, body mass index (BMI), total body fat (TBF), and serum lipid profiles in 790 apparently healthy adolescents. TBF was assessed using a body fat analyzer. Serum concentrations of triglyceride, to...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：OBJECTIVE:MiR-15 is involved in modulating many cellular processes that are normally destroyed in disease. The regulation of the cell cycle, angiogenesis, and responses to cellular stress are notable processes influenced by MiR-15. MiR-15 plays a critical role in the model of inflammation-related angiogenesis defects. ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2019-09-01 00:00:00
abstract：:A case of a 40 year-old woman with Hodgkin's disease who developed acute non-lymphocytic leukemia (ANLL) accompanied by several abnormal chromosomal findings is illustrated. A very unusual coincidental finding was the coexistence of Hodgkin's lymphoma and ANLL in the same lymph node. ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Increased lipid peroxidation (LP) occurs in the presence of various transition, metal ions, including copper and iron, as well as various heme moieties. In addition, irradiation significantly increases LP. The degree of LP, as determined by the liquid chromatographic measurement of plasma malondialdehyde (MDA), can be...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:This study assessed the progression of renal damage in obese Zucker rats in response to deoxycorticosterone acetate (DOCA)-salt-induced hypertension. Renal damage was evaluated by light microscopy and urine analysis at weekly intervals during the developmental phase of DOCA-salt hypertension and once during the platea...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Intravascular large B-cell lymphoma (IVLBCL) is a rare variant of extranodal diffuse large Bcell lymphoma with only a few more than 300 cases reported. It is characterized as lymphoma cells confined to the lumina of small vessels, so patients usually do not present with masses or lymphadenopathy. Clinical presentation...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2011-07-01 00:00:00
abstract：:In the past, necrotizing vasculitis has been considered to be one of the dominant intrarenal vascular abnormalities in systemic lupus erythematosus (SLE). To test the validity of this statement, 70 consecutive renal biopsies from patients with SLE were reviewed. Light microscopy (LM) and immunofluorescence (IF) studie...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：BACKGROUND:Human mammary tumor virus (HMTV) is 90-95% homologous to mouse mammary tumor virus, one of the causal agents of murine mammary tumors. Although HMTV has been frequently detected in human breast cancers, its clinical and prognostic value remains unknown. METHODS:In the present study, we analyzed HMTV infecti...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2019-03-01 00:00:00
abstract：:This review considers the most recent developments concerning the metabolism and homeostasis of calcium and phosphorus. The kinetics of the distribution of calcium, theories of calculus formation, hypercalcemia and hypocalcemia are discussed, as well as the role of parathyroid hormone, thyrocalcitonin and 1,25 dihydro...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1975-05-01 00:00:00
abstract：:This study was to examine the inhibitory effect of matrine on the proliferation and metastasis of gastric cancer cells, and to explore the possible mechanisms involved in these processes. MTT was used to evaluate the proliferation ability of SGC7901 cells. A two and three-dimensional cell migration assay were performe...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Various assays for detecting high-risk human papillomavirus (HR HPV) have been introduced recently, including the Abbott RealTime High-Risk HPV assay. We sought to compare the performance of Abbott PCR to Hybrid Capture 2 for the detection of HR HPV. METHODS:A total of 941 cervical swab specimens were obtai...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：:A brief historical review is presented of the development of bovine serum albumin as a standard for the determination of serum total protein. Bovine serum albumin is now available as a standard reference material from the National Bureau of Standards. Also reviewed is the present status of the immunoglobulin standards...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:Artifactual distortion of hematopoietic elements in histologic preparations and smears of bone marrow aspirates simulating metastatic small cell carcinoma has been recently described. The clinical, pathologic, and immunohistochemical features in 12 such cases have been studied by us. In all these cases, bone marrow sm...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Widespread drug abuse, a comparatively recent medicosocial phenomenon, presents protean clinical patterns and challenging diagnostic problems daily that mimic classical medical syndromes. However, few reports have delineated predominantly neurologic syndromes associated with drug abuse. Five patients were observed ill...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:The pattern of excretion of urinary acid mucopolysaccharides (AMPS) has been helpful to establish the diagnosis of mucopolysaccharidoses. The importance of urine analysis for AMPS and the specific enzyme assays is exemplified in a 3 1/2 year old Caucasian male with severe mental retardation, small stature, thoracolumb...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：:We previously reported that proper dilution of sera that contain IgG monoclonal proteins (M-proteins) is necessary for accurate quantitation of serum albumin and M-protein concentrations separated by serum protein electrophoresis (SPE) using the Beckman PARAGON agarose electrophoresis system. We now report the signifi...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:A fifteen-year-old girl was treated with isoniazid (INH) for latent tuberculosis infection (LTBI), and subsequently developed epigastric pain, vomiting, and jaundice after three months of treatment. Acute fulminant hepatic failure was diagnosed. INH was stopped, and she received N-acetyl cysteine and Vitamin K. Liver ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The purpose of this study was to evaluate immunoassay methods for the measurement of serum cardiac creatine kinase isoenzyme (CK-MB) with respect to sensitivity and specificity. The CK-MB electrophoretic assay (Helena Laboratories) was used as the reference. Two principles of immunoassay were included in the evaluatio...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:The presence of effusion in association with multiple myeloma or macroglobulinemia is an unusual finding which generally occurs late in the course of the disease. Occasionally, cytoligic detection is a diagnostic problem. In both conditions, the fluid is characrerized by a high specific gravity and high protein conten...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
