Abstract：
:Recombinant human apolipoprotein E3 (apoE), purified from E. coli, inhibited the proliferation of several cell types, including endothelial cells and tumor cells in a dose- and time-dependent manner. ApoE inhibited both de novo DNA synthesis and proliferation as assessed by an increase in cell number. Maximal inhibition of cell growth by apoE was achieved under conditions where proliferation was dependent on heparin-binding growth factors. Thus, at low serum concentrations (0-2.5%) basic fibroblast growth factor (bFGF) stimulated the proliferation of bovine aortic endothelial (BAE) cells severalfold. The bFGF-dependent proliferation was dramatically inhibited by apoE with an IC50 approximately 50 nM. Under conditions where cell proliferation was mainly serum-dependent, apoE also suppressed growth but required higher concentrations to be effective (IC50 approximately 500 nM). ApoE also inhibited growth of bovine corneal endothelial cells, human melanoma cells, and human breast carcinoma cells. The IC50 values obtained with these cells were generally 3-5 times higher than with BAE cells. Inhibition of cell proliferation by apoE was reversible and dependent on the time of apoE addition to the culture. In addition, apoE inhibited the chemotactic response of endothelial cells that were induced to migrate by a gradient of soluble bFGF. Inhibition of cell proliferation by apoE may be mediated both by competition for growth factor binding to proteoglycans and by an antiadhesive activity of apoE. The present results demonstrate that apoE is a potent inhibitor of proliferation of several cell types and suggest that apoE may be effective in modulating angiogenesis, tumor cell growth, and metastasis.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Vogel T,Guo NH,Guy R,Drezlich N,Krutzsch HC,Blake DA,Panet A,Roberts DDdoi
10.1002/jcb.240540306subject
Has Abstractpub_date
1994-03-01 00:00:00pages
299-308issue
3eissn
0730-2312issn
1097-4644journal_volume
54pub_type
杂志文章abstract：:Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. We investigated the effect of a prior 30 days voluntary exercise protocol on STZ-diabetic CF1 mice. Glycemia, and the liver and skeletal muscle glycogen, mitochondrial function, and redox status were analyzed up to 5 days after STZ injection. An...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25658
更新日期：2017-04-01 00:00:00
abstract：:Primary chicken mesenchymal cells from limb buds and vertebral chondrocytes have been used to study the changes that occur in alternative mRNA splicing of fibronectin exon EIIIA during chondrogenesis. The mesenchymal cell phenotype (exon EIIIA included) and chondrocyte phenotype (exon EIIIA excluded) were preserved in...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(20000201)76:2<341::aid-jcb
更新日期：1999-12-01 00:00:00
abstract：:During the past several years, numerous laboratories have reported isolation and purification of proteinase inhibitors from human urine. Many of these molecules were incompletely characterized and some of them may have been artifacts in part because of harsh procedures used for their isolation. Consequently, there is ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240500303
更新日期：1992-11-01 00:00:00
abstract：:The aim of the present study was to test the hypothesis that the decreased renal tubular reabsorption of calcium observed in estrogen deficiency is associated with a local regulation of either PTHrP or PTH/PTHrP receptor genes in the kidney. Rats were randomly sham-operated (S) or ovariectomized receiving either vehic...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Preserving self-renewal, multipotent capacity, and large-scale expansion of highly functional progenitor cells, including endothelial progenitor cells (EPCs), is a controversial issue. These current limitations, therefore, raise the need of developing promising in vitro conditions for prolonged expansion of EPCs witho...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25492
更新日期：2016-08-01 00:00:00
abstract：:Vacuolar H+ -ATPases (V-ATPases) are ubiquitous multisubunit proton pumps responsible for organellar pH maintenance. Mutations in the a3 subunit of V-ATPases cause autosomal recessive osteopetrosis, a rare disease due to impaired bone resorption. Patients with osteopetrosis also display dental anomalies, such as ename...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25986
更新日期：2017-10-01 00:00:00
abstract：:The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological control and are compromised with the onset and progression of ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.21813
更新日期：2008-08-15 00:00:00
abstract：:The cellular distribution and nature of proteoglycans synthesised by human breast cancer cells in culture were studied. Proteoglycans were labelled with [35S] sulfate, purified, and characterised after ion-exchange chromatography followed by gel-filtration chromatography and treatment with glycosaminoglycan degrading ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-03-15 00:00:00
abstract：:This laboratory recently reported the development of a biotin-cellulose/streptavidin affinity chromatography method based on the DNase I sensitivity of active chromatin to isolate a DNA fraction from murine erythroleukemia (MEL) cells that is more than 15-fold enriched in active genes (Dawson et al.: Journal of Biolog...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240460210
更新日期：1991-06-01 00:00:00
abstract：:The sequences from several independent cDNA clones encoding the chicken vitamin D receptor as well as primer extension assay have clearly delineated the 5' terminus and the transcriptional start site. Screening a chicken genomic library produced genomic clones containing vitamin D receptor (VDR) gene fragments. Restri...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Osteosarcoma is the most common malignant bone tumor in children and adolescents with high rate of incidence, high frequency of recurrence, and high degree of metastasis. This study aimed to investigate the effects of long noncoding RNA antisense ncRNA in the abundant in neuroepithelium area (ANA)/B-cell translocation...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26671
更新日期：2018-08-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small, non-coding RNAs that participate in the regulation of gene expression. Although many studies have demonstrated the involvement of miR-17-5p in different cancers, little is known to its function in ovarian cancer. In this study, we demonstrated that overexpression of miR-17-5p was able to ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25060
更新日期：2015-06-01 00:00:00
abstract：:Preeclampsia is a pathologic complication of pregnancy, associated with increased apoptosis in the cytotrophoblasts as the main cause of this disorder. Caspase-3 is a key apoptosis-related enzyme that both mitochondrial and death receptor apoptotic pathways can activate. In this study, we aimed to investigate the effe...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26869
更新日期：2018-08-01 00:00:00
abstract：:Metastatic cancer is a complex positive feedback loop system. Such as system has a tendency to acquire extreme robustness. Signaling pathways controlling that robustness can fail completely if an essential element from the signaling is removed. That element is a locus of fragility. Targeting that locus represents the ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.21777
更新日期：2008-08-15 00:00:00
abstract：:With a signal trap method, we previously identified stromal interaction molecule (STIM: originally named as SIM) as a protein, which has a signal peptide in 1996. However, recent works have accumulated evidences that STIM1 and STIM2 reside in endoplasmic reticulum (ER) and that both mainly sense ER Ca(2+) depletion, w...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22910
更新日期：2011-01-01 00:00:00
abstract：:Diacylglycerol kinases (DGKs) attenuate diacylglycerol signaling by converting this lipid to phosphatidic acid (PA). The nine mammalian DGKs that have been identified are widely expressed, but each isoform has a unique tissue and subcellular distribution. Their kinase activity is regulated by mechanisms that modify th...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.20704
更新日期：2006-02-15 00:00:00
abstract：:The PML protein and PML nuclear bodies (PML-NB) are implicated in multiple cellular functions relevant to tumor suppression, including DNA damage response. In most cases of acute promyelocytic leukemia, the PML and retinoic acid receptor alpha (RARA) genes are translocated, resulting in expression of oncogenic PML-RAR...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24050
更新日期：2012-05-01 00:00:00
abstract：:Mechanical loading of bone generates fluid flow within the mineralized matrix that exerts fluid shear stress (FSS) on cells. We examined effects of FSS on receptor activator of nuclear factor kappa B ligand (RANKL), a critical factor for osteoclast formation. Primary murine osteoblasts were subjected to pulsatile FSS ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20864
更新日期：2006-08-01 00:00:00
abstract：:Enhanced phosphorylation of the ribosomal protein s6 kinase, p70(s6k), and the translational repressor, 4E-BP1, are associated with either insulin-induced or amino acid-induced protein synthesis. Hyperphosphorylation of p70(s6k) and 4E-BP1 in response to insulin or amino acids is mediated through the mammalian target ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/1097-4644(20001201)79:3<427::aid-jcb80>3.0
更新日期：2000-09-07 00:00:00
abstract：:ERK1 and ERK2 share a great deal of homology and have been presumed to have similar functions. Available antibodies recognize both isoforms making the elucidation of functional differences challenging. Mitogen-activated protein (MAP) kinase networks are commonly depicted in the literature as linear and sequential phos...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25896
更新日期：2017-08-01 00:00:00
abstract：:Myelin basic protein (MBP) is a major component of the myelin sheath whose production is developmentally controlled during myelinogenesis. Earlier studies have indicated that programmed expression of the MBP gene is regulated at the level of transcription. Evidently, the MB1 regulatory motif located between nucleotide...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(19970915)66:4<524::aid-jcb
更新日期：1997-09-15 00:00:00
abstract：:Fibrosis, the deadly pathological manifestation of an abnormal tissue remodeling in any organ due to excessive collagen deposition, is associated with a wide variety of organ failure-related human diseases. Chronic stress or repeated injury in a particular organ induces abnormal molecular signals that lead to super-ac...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24820
更新日期：2014-09-01 00:00:00
abstract：:Recently, glioma stem cells have been identified as the main cause of glioma propagation and recurrence and a number of several cell markers have been indicated as putative GSC markers. In the present work, a retrospective study to evaluate the prognostic potential of ability to generate GSCs in our series of 15 gliob...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25043
更新日期：2015-05-01 00:00:00
abstract：:We investigated the association of DNA polymerase and DNA primase activity with the nuclear matrix in HeLa S3 cells diluted with fresh medium after having been cultured without any medium change for 7 days. Flow cytometric analysis demonstrated that just before dilution about 85% of the cells were in the G1 phase of t...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(19981001)71:1<11::aid-jcb2
更新日期：1998-10-01 00:00:00
abstract：:The active vitamin D metabolite 1,25-dihydroxyvitamin D3 [1,25-D3] is thought to promote many of its actions through interaction with a specific intracellular receptor. The discovery of such receptors in monocytes and activated lymphocytes has led investigators to evaluate the role of the hormone on the immune system....
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.240490106
更新日期：1992-05-01 00:00:00
abstract：:Epidermal growth factor (EGF)-receptor is a 170 kDa transmembrane glycoprotein that contains a ligand-binding external domain, and a catalytically active cytoplasmic region. The expression of this receptor as well as a 100 kDa homolog that contains only the external domain is enhanced by the ligand EGF. EGF acts at tr...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240440106
更新日期：1990-09-01 00:00:00
abstract：:Berberine (BBR) is an isoquinoline alkaloid derived from various medicinal herbs. Previous studies have suggested that BBR exerts antimicrobial, antitumor, and antidiabetic effects and can be used to treat Helicobacter pylori-induced chronic gastritis. However, the exact mechanism by which BBR inhibits H. pylori infec...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26681
更新日期：2018-07-01 00:00:00
abstract：:Androgen-regulated genes (ARG) are implicated in normal and neoplastic growth of the prostate. Recently, we reported genomic amplification and/or overexpression of a previously known neurotrophic factor, prosaposin, in androgen-independent (AI) or metastatic prostate cancer (PCa) cells and tissues. Prosaposin and/or i...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21207
更新日期：2007-06-01 00:00:00
abstract：BACKGROUND AND AIM:Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, is very critical to health maintenance, for a wide range of human chronic diseases, including cancers. AMP-activated protein kinase (AMPK) has been implicated in the activation of autophagy in distinct tissues. This study wa...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27491
更新日期：2018-10-30 00:00:00
abstract：:Vascular smooth muscle cells (SMCs), the major cellular constituent of an artery, synthesize the bulk of fibrillar collagens, including type V/XI, which regulates heterotypic collagen fibril assembly. Basic fibroblast growth factor (bFGF) is a heparin-binding polypeptide growth factor that has been implicated in impor...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(19980201)68:2<247::aid-jcb
更新日期：1998-02-01 00:00:00
