Abstract：
:Preserving self-renewal, multipotent capacity, and large-scale expansion of highly functional progenitor cells, including endothelial progenitor cells (EPCs), is a controversial issue. These current limitations, therefore, raise the need of developing promising in vitro conditions for prolonged expansion of EPCs without loss of their stemness feature. In the current study, the possible role of three different natural extracellular substrates, including collagen, gelatin, and fibronectin, on multiple parameters of EPCs such as cell morphology, phenotype, clonogenic, and vasculogenic properties was scrutinized. Next, EPCs from GFP-positive mice were pre-expanded on each of these ECM substrates and then systemically transplanted into sublethaly irradiated mice to analyze the potency of these cells for marrow reconstitution. Our results revealed considerable promise for fibronectin for EPC expansion with maintenance of stemness characteristics, whereas gelatin and collagen matrices directed the cells toward a mature endothelial phenotype. Transplantation of EPCs pre-expanded on fibronectin resulted in widespread distribution and appropriate engraftment to various tissues with habitation in close association with the microvasculature. In addition, fibronectin pre-expanded cells were gradually enriched in the bone marrow after transplantation, resulting in marrow repopulation and hematologic recovery, leading to improved survival of recipient mice whereas gelatin- and collagen-expanded cells failed to reconstitute the bone marrow. This study demonstrated that, cell characteristics of in vitro expanded EPCs are determined by the subjacent matrix. Fibronectin-expanded EPCs are heralded as a source of great promise for bone marrow reconstitution and neo-angiogenesis in therapeutic bone marrow transplantation. J. Cell. Biochem. 117: 1934-1946, 2016. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Siavashi V,Nassiri SM,Rahbarghazi R,Vafaei R,Sariri Rdoi
10.1002/jcb.25492subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1934-46issue
8eissn
0730-2312issn
1097-4644journal_volume
117pub_type
杂志文章abstract：:Enhanced phosphorylation of the ribosomal protein s6 kinase, p70(s6k), and the translational repressor, 4E-BP1, are associated with either insulin-induced or amino acid-induced protein synthesis. Hyperphosphorylation of p70(s6k) and 4E-BP1 in response to insulin or amino acids is mediated through the mammalian target ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/1097-4644(20001201)79:3<427::aid-jcb80>3.0
更新日期：2000-09-07 00:00:00
abstract：:Osteogenic differentiation refers to the process of bone formation and remodeling, which is controlled by complex molecular mechanisms. Activin A receptor type I (ACVR1) is reported to be associated with osteogenic differentiation. However, the underlying molecular mechanism remains elusive. Therefore, this study eval...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28100
更新日期：2018-12-16 00:00:00
abstract：:Toxoplasma gondii an obligate intracellular parasite causes toxoplasmosis in homeothermic animals. Host invasion of this parasite is mediated by the formation of Moving Junction (MJ) complex which encompasses a network of microneme and Rhoptry Neck proteins (RONs) 2/4/5/8. Among these proteins, RON4 is the only cytoso...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26480
更新日期：2018-04-01 00:00:00
abstract：:Formyl peptide receptors (FPRs) are a family of classical chemoattractant receptors. Although FPRs are mainly expressed in phagocytic innate immune cells including monocytes/macrophages and neutrophils, recent reports demonstrated that additional different cell types such as T-lymphocytes and several non-immune cells ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.25877
更新日期：2017-06-01 00:00:00
abstract：:The adipocyte secreted hormone leptin (OB) and its receptor (OB-R) are key regulators of mammalian body weight homeostasis. Two predominant isoforms of OB-R have been described: long form (OB-R(L)) characterized as a signal transducing receptor that is highly expressed in specific nuclei of the hypothalamus; and a sho...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:In this experiment, the cross-talk betweenNotch and the NF-κB signaling pathway was examined to reveal the mechanism of slowing down the type II collagen (ColII) and aggrecan degeneration affected by inflammatory cytokines. The expression levels of ColII and aggrecan in the intervertebral disc were observed through im...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27504
更新日期：2018-10-26 00:00:00
abstract：:In the heart, insulin-like growth factor-1 (IGF-1) is a peptide with pro-hypertrophic and anti-apoptotic actions. The pro-hypertrophic properties of IGF-1 have been attributed to the extracellular regulated kinase (ERK) pathway. Recently, we reported that IGF-1 also increases intracellular Ca(2+) levels through a pert...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24712
更新日期：2014-04-01 00:00:00
abstract：:SMAD ubiquitination regulatory factor 1 (SMURF1) has been described as a tumor suppressor in multiple aggressive cancers. Nevertheless, the potential role of SMURF1 in ovarian cancer invasion and epithelial-to-mesenchymal transition (EMT) remains unclear. The aim of this study was to evaluate the efficacy of SMURF1 on...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28354
更新日期：2019-06-01 00:00:00
abstract：:Bladder cancer is the most common malignancy with high recurrence. Currently, the long noncoding RNAs (lncRNAs) have been suggested to play vital roles in the pathogenesis of bladder cancer. The present study investigated the role of lncRNA MIR503 host gene (MIR503HG) in the pathogenesis of bladder cancer by using bot...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28373
更新日期：2019-06-01 00:00:00
abstract：:LipN (Rv2970c) belongs to the Lip family of M. tuberculosis H37Rv and is homologous to the human Hormone Sensitive Lipase. The enzyme demonstrated preference for short carbon chain substrates with optimal activity at 45°C/pH 8.0 and stability between pH 6.0-9.0. The specific activity of the enzyme was 217 U/mg protein...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25285
更新日期：2016-02-01 00:00:00
abstract：:lncRNAs are responsible for a variety of diseases, including gastric cancer (GC). Many recent studies have reported that lncRNAs can serve as crucial regulators of various genes. Nevertheless, the biological function of lncRNA damage induced noncoding (DINO) remained poorly investigated in GC. Therefore, in our presen...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28394
更新日期：2019-02-18 00:00:00
abstract：:With a signal trap method, we previously identified stromal interaction molecule (STIM: originally named as SIM) as a protein, which has a signal peptide in 1996. However, recent works have accumulated evidences that STIM1 and STIM2 reside in endoplasmic reticulum (ER) and that both mainly sense ER Ca(2+) depletion, w...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22910
更新日期：2011-01-01 00:00:00
abstract：:Metastatic cancer is a complex positive feedback loop system. Such as system has a tendency to acquire extreme robustness. Signaling pathways controlling that robustness can fail completely if an essential element from the signaling is removed. That element is a locus of fragility. Targeting that locus represents the ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.21777
更新日期：2008-08-15 00:00:00
abstract：:Long noncoding RNA (lncRNA) maternally expressed 3 (MEG3) has been implicated as a tumor suppressor gene in several human cancer types. However, little is known regarding its involvement and potential mechanism in human breast cancer. In this study, we explored the effect of MEG3 on the growth of human breast cancer c...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27982
更新日期：2019-04-01 00:00:00
abstract：:Receptor tyrosine kinases (RTKs) expression and the growth factor such as platelet-derived growth factor (PDGF) and their receptors have been considered relevant in the process of angiogenesis and dissemination in esophageal squamous cell carcinoma (ESCC). Small glutamine-rich tetratricopeptide repeat-containing prote...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24641
更新日期：2014-01-01 00:00:00
abstract：:Lysozyme is expressed in serous, but not mucous, cells of the tracheobronchial glands and thereby constitutes a marker of the serous cell lineage in these glands. To identify DNA regulatory elements and transcription factors mediating the commitment of progenitor cells to the serous cell lineage, we have characterized...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4644(19960601)61:3%3C350::AID-J
更新日期：1996-06-01 00:00:00
abstract：:Gastric cancer (GC) is the fourth common cancer and second leading cause of cancer-related mortality in the world. WD repeat domain 5 (WDR5) has been identified that its functions as an important role in various biological functions through the epigenetic regulation of gene transcription. However, the oncogenic effect...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26491
更新日期：2018-04-01 00:00:00
abstract：:Previously we have shown (Hebert et al. [1999] J. Cell Biochem. 73:248-258) that among many cell lines the CBP2 gene product, Hsp47, eludes its retention receptor, erd2P, resulting in the appearance of Hsp47 on the cell surface associated with the tetraspanin protein CD9. Since Hsp47 possesses a highly restricted bind...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Circular RNA (circRNA) is a new noncoding RNAs and plays an important role in many pathological processes. Recently, studies have shown that circular RNA_PRKCI (circ-PRKCI) regulates cell proliferation and cell migration of tumor cells. Esophageal carcinoma is a highly malignant digestive tract tumor, which is divided...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28285
更新日期：2019-06-01 00:00:00
abstract：:Nucleic acids and regulatory proteins are architecturally organized in nuclear microenvironments. The compartmentalization of regulatory machinery for gene expression, replication and repair, is obligatory for fidelity of biological control. Perturbations in the organization, assembly and integration of regulatory mac...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21846
更新日期：2008-08-15 00:00:00
abstract：:Magnolol, a substance purified from the bark of Magnolia officialis, inhibits cell proliferation and induces apoptosis in a variety of cancer cells. The aim of this study was to study the effects of magnolol on CGTH W-2 thyroid carcinoma cells. After 24 h treatment with 80 microM magnolol in serum-containing medium, a...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21100
更新日期：2007-07-01 00:00:00
abstract：:c-fos gene has a close relationship with the osteoblasts. Mechanical signal effect on osteoblasts would change the expression level of c-fos. Authors introduce the signal pathways of four cis-response elements on the promoter of c-fos, that is, CRE (cAMP responsive element), FAP-1 (Fbs-AP-1 site), SRE (serum response ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22058
更新日期：2009-04-01 00:00:00
abstract：:Genome-wide screening of the yeast Saccharomyces cerevisiae knockout collection was used to characterize chemical-genetic interactions of cycloheximide (CHX). The results showed that while the act1Δ mutant was the only deletion mutant in the heterozygous essential gene deletion collection that showed hypersensitivity ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25601
更新日期：2016-12-01 00:00:00
abstract：:Skeletal muscle atrophy is a well-known adverse effect of chronic treatment with glucocorticoids and it also occurs when stress conditions such as sepsis and cachexia increase the release of endogenous glucocorticoids. Although the mechanisms of action of these hormones have been elucidated, the possible molecular mec...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25483
更新日期：2016-08-01 00:00:00
abstract：:Vascular pathologies induced by ischemia/reperfusion involve the production of reactive oxygen species (ROS) that in part cause tissue injury. The production of ROS that occurs upon reperfusion activates specific second messenger pathways. In diabetic retinopathy there is a characteristic loss of the microvascular per...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Cancer of the ovary is the leading cause of death from gynecologic malignancy. To understand better these aggressive tumors, the development of in vitro models to study human ovarian cancer is critical. However, the establishment of long-term cell lines has been difficult, due to the generalized poor survival of patie...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.240510307
更新日期：1993-03-01 00:00:00
abstract：:The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three-dimensional pattern of the testis cells arrangement, which is directly re...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28995
更新日期：2019-10-01 00:00:00
abstract：:Cyclin-dependent kinases (cdks) are the catalytic subunits of a large family of serine/threonine protein kinases whose best-characterized members are key regulators of eukaryotic cell cycle progression. They are activated by binding to regulatory subunits generally termed as cyclins. Cdk10 is a cdc2-related kinase tha...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20981
更新日期：2006-10-15 00:00:00
abstract：:Basic fibroblast growth factor (bFGF) is found in a variety of cells and tissues. We have previously shown that bFGF is a transforming growth factor, but only when fused to a signal peptide (sp-bFGF). Cells expressing the native bFGF are tumorigenic in nude mice only, where the tumors form at a low frequency and grow ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240390103
更新日期：1989-01-01 00:00:00
abstract：:TNF-a is an important cytokine mediator of inflammation which suggests that inhibition of TNF activity may provide potential for clinical application. Recent data indicated that treatment of both human and mouse cells with Kavain significantly modulates P. gingivalis- and LPS-induced TNF-α expression. In order to obta...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26805
更新日期：2018-07-01 00:00:00
