ACVR1-knockout promotes osteogenic differentiation by activating the Wnt signaling pathway in mice.


:Osteogenic differentiation refers to the process of bone formation and remodeling, which is controlled by complex molecular mechanisms. Activin A receptor type I (ACVR1) is reported to be associated with osteogenic differentiation. However, the underlying molecular mechanism remains elusive. Therefore, this study evaluates the function of ACVR1 in osteogenic differentiation through the Wnt signaling pathway. The expression of osteocalcin (Oc) and osterix together with osteogenic differentiation and mineralization was examined in ACVR1-knockout (KO) mouse. Furthermore, the Wnt signaling pathway was inhibited in bone marrow stromal cells (BMSCs) of mice to explore the role of the Wnt signaling pathway in osteogenic differentiation by means of alkaline phosphatase (ALP) activity detection and evaluation of mineralized nodules and calcium content. Subsequently, the effect of ACVR1 on the Wnt signaling pathway was assessed by determining the expression of ACVR1, β-catenin, glycogen synthase kinase 3 β (GSK3β), dickkopf-related protein 1 (DKK1), and frizzled class receptor 1 (FZD1). Both their effects on osteogenic differentiation were further evaluated by determination of Oc, osterix, and Runx2 expression. AVCR1 KO mice exhibited increased Oc and osterix expression and promoted bone resorption and formation. ACVR1-knockout was observed to activate the Wnt signaling pathway with an increase of β-catenin and reductions in GSK3β, DKK1, and FZD1. With the inhibited Wnt signaling pathway expression of Oc, osterix, and Runx2 was decreased, and ALP activity, mineralized nodule, and calcium content in cellular matrix were decreased as well, indicating that inactivation of the Wnt signaling pathway reduced the differentiation of BMSCs into osteoclasts. These findings indicate that ACVR1-knockout promotes osteogenic differentiation by activating the Wnt signaling pathway in mice.


J Cell Biochem


Wang Y,Sun JC,Wang HB,Xu XM,Kong QJ,Wang YJ,Zheng B,Shi GD,Shi JG




Has Abstract


2018-12-16 00:00:00






  • NS5ATP9 Promotes Beclin 1-Dependent Starvation-Induced Autophagy of Hepatoblastoma Cells.

    abstract::NS5ATP9, a gene up-regulated by NS5A, plays a crucial oncogenic role in several types of human tumours. However, the underlying mechanisms remain unclear. Autophagy, an evolutionarily conserved catabolic process, maintains cellular homeostasis under stress conditions, such as starvation, and plays a crucial role in tu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Quan M,Liu S,Wang Q,Li G,Zhang Y,Feng S,Liang J,Cheng J

    更新日期:2015-08-01 00:00:00

  • Extracellular matrix-dependent regulation of angiogenin expression in human placenta.

    abstract::Knowledge of the rapidly developing hierarchy of controls affecting vascular development in placenta is required to understand how the growth factors and their receptor-mediated signals actually produce vessels. At the cell biological level, these events clearly require stable interactions between the cells, and cells...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Rajashekhar G,Loganath A,Roy AC,Chong SS,Wong YC

    更新日期:2005-09-01 00:00:00

  • The activation of matrix metalloproteinase-2 induced by protein kinase C alpha in decidualization.

    abstract::This study investigated the protein kinase C (PKC) and matrix metalloproteinase-2 (MMP-2) in the development of deciduomata in pseudo-pregnant and pregnant rats. The results showed that the expression of MMP-2 was significantly increased from day 2 to day 5 in pseudo-pregnancy and from day 7 to day 9 in pregnancy. To ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tsai JH,Hwang JM,Ying TH,Shyu JC,Tsai CC,Hsieh YS,Wang YW,Liu JY,Kao SH

    更新日期:2009-10-15 00:00:00

  • Investigating the neuroprotective mechanism of action of a CDK5 inhibitor by phosphoproteome analysis.

    abstract::Small molecule inhibitors of cyclin-dependent kinase 5 (CDK5) protect neurons from cell death following various insults. To elucidate the cellular mechanism of action we investigated changes in protein phosphorylation in cultured rat cerebellar granule neurons after administration of the CDK5 inhibitor Indolinone A. B...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Gillardon F,Schrattenholz A,Sommer B

    更新日期:2005-07-01 00:00:00

  • Comparative efficacy and safety of antiplatelet agents in cerebral ischemic disease: A network meta-analysis.

    abstract::We performed a network meta-analysis (NMA) to enhance the corresponding evidence of the relative efficacy and safety of different antiplatelet agents in cerebral ischemic disease. PubMed and EMBASE were searched systematically for relevant studies. Outcomes were compared using odds ratios and 95% credible intervals. E...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,meta分析


    authors: Wa D,Zhu P,Long Z

    更新日期:2019-06-01 00:00:00

  • Sequence analysis and in vitro transcription of portions of the Epstein-Barr virus genome.

    abstract::The 17,180 base-pair Eco-RI-C fragment of Epstein-Barr virus has been sequenced in its entirety. This same fragment has also been analyzed for RNA polymerase II promoters, which are active in a soluble in vitro assay. These data are compared to the availability of predicted open reading frames and potential nucleotide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Deininger PL,Bankier A,Farrell P,Baer R,Barrell B

    更新日期:1982-01-01 00:00:00

  • Long noncoding RNAs C2dat1 enhances vascular smooth muscle cell proliferation and migration by targeting MiR-34a-5p.

    abstract::Deregulated proliferation of vascular smooth muscle cells (VSMCs) is one common phenomenon of atherosclerosis progression. Long noncoding RNAs (lncRNAs) are one group of noncoding RNAs that play essential roles in many cell biological processes, including cell development, growth, and migration. However, the role of a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wang H,Jin Z,Pei T,Song W,Gong Y,Chen D,Zhang L,Zhang M,Zhang G

    更新日期:2019-03-01 00:00:00

  • Association of TNF-308 G>A polymorphism located in tumor necrosis factor a with the risk of developing cervical cancer and results of pap smear.

    abstract::Tumor necrosis factor a (TNFa) is an inflammatory cytokine that plays a crucial role in the immune response and the progression of cervical lesions. There is a growing body of data evaluating the value of a genetic variant in the TNFa gene with the risk of developing cervical cancer. The aim of this study was to explo...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Babapour N,Mehramiz M,Rastgar Moghadam A,Behboodi N,Yousefi Z,Maftouh M,Talebian S,Khazaei M,Jafarian A,Sharifi-Sistani N,Avan A,Hasanzadeh M

    更新日期:2019-04-01 00:00:00

  • Evaluate the Efficacy and Safety of Anti-Epileptic Medications for Partial Seizures of Epilepsy: A Network Meta-Analysis.

    abstract::Epilepsy is a brain and neurological disorder with high prevalence. It was reported that more than 70% of epileptic seizures were controlled by anti-epileptic medications, whereas the lack of evidence with respect to head-to-head comparisons motivated researchers to seek alternative approaches that are able to provide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,meta分析,评审


    authors: Zhao T,Feng X,Liu J,Gao J,Zhou C

    更新日期:2017-09-01 00:00:00

  • MeCP2 epigenetically regulates alpha-smooth muscle actin in human lung fibroblasts.

    abstract:BACKGROUND:A critical feature for fibroblasts differentiation into myofibroblasts is the expression of alpha-smooth muscle actin (α-SMA) during the tissue injury and repair process. The epigenetic mechanism, DNA methylation, is involved in regulating α-SMA expression. It is not clear how methyl-CpG-binding protein 2 (M...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Xiang Z,Zhou Q,Hu M,Sanders YY

    更新日期:2020-07-01 00:00:00

  • Caspase inhibition and N6-benzyladenosine-induced apoptosis in HL-60 cells.

    abstract::As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N(6)-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. Howeve...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mlejnek P

    更新日期:2001-01-01 00:00:00

  • Fibroblast growth factor-2 promotes catabolism via FGFR1-Ras-Raf-MEK1/2-ERK1/2 axis that coordinates with the PKCδ pathway in human articular chondrocytes.

    abstract::Fibroblast growth factor 2 (FGF-2) has been found to play an anti-anabolic and/or a catabolic role in adult human articular cartilage via regulation of multiple signaling pathways. Upon FGF-2 stimulation, a molecular crosstalk between the mitogen activated protein kinase (MAPK) and protein kinase C δ (PKCδ) pathways a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yan D,Chen D,Im HJ

    更新日期:2012-09-01 00:00:00

  • Automated Analysis of Cell Cycle Phase-Specific DNA Damage Reveals Phase-Specific Differences in Cell Sensitivity to Etoposide.

    abstract::The comet assay is one of the most widely used approaches for detecting DNA damage; generally, it provides information on the cell population-averaged level of DNA damage. Here, we present an automatic technique for easy measurement of standard comet characteristics and an annotation of the cell cycle phase of each co...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Luzhin AV,Velichko AK,Razin SV,Kantidze OL

    更新日期:2016-10-01 00:00:00

  • 3,4,5-trihydroxybenzaldehyde from Geum japonicum has dual inhibitory effect on matrix metalloproteinase 9; inhibition of gelatinoytic activity as well as MMP-9 expression in TNF-alpha induced HASMC.

    abstract::The matrix metalloproteinases (MMP-9 and MMP-2) production and smooth muscle cell (SMC) migration may play key roles in the pathogenesis of atherosclerotic lesions. In particular, the cancer cell invasion and SMC migration through vascular wall were shown to be directly associated with inducible MMP-9 expression. Prev...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Suh SJ,Cho KJ,Moon TC,Chang HW,Park YG,Kim CH

    更新日期:2008-10-01 00:00:00

  • Biochemical evidence on the potential role of methyl mercury in hepatic glucose metabolism through inflammatory signaling and free radical pathways.

    abstract::Methylmercury (MeHg) is an extremely important environmental toxicant posing serious health risks to human health and a big source of environmental pollutant. Numerous evidence available showing a link between nervous system toxicity and MeHg exposure. Other forms of mercury are reason of metabolic toxic effects and a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Maqbool F,Bahadar H,Hassani S,Niaz K,Baeeri M,Rahimifard M,Ghasemi-Niri SF,Abdollahi M

    更新日期:2019-09-01 00:00:00

  • Immature and mature megakaryocytes enhance osteoblast proliferation and inhibit osteoclast formation.

    abstract::Recent data suggest that megakaryocytes (MKs) play a role in skeletal homeostasis. In vitro and in vivo data show that MKs stimulate osteoblast (OB) proliferation and inhibit osteoclast (OC) formation, thus favoring net bone deposition. There are several mouse models with dysregulated megakaryopoiesis and resultant hi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ciovacco WA,Cheng YH,Horowitz MC,Kacena MA

    更新日期:2010-03-01 00:00:00

  • Giant cell tumor of bone: a unique paradigm of stromal-hematopoietic cellular interactions.

    abstract::Giant cell tumor of bone is a progressive, potentially malignant process which destroys skeletal tissue by virtue of its osteoclast complement. As a biological entity it provides a unique natural model of bone resorption by osteoclasts whose recruitment and development is controlled by a neoplastic population of fibro...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Robinson D,Einhorn TA

    更新日期:1994-07-01 00:00:00

  • NF-kappaB activation plays an antiapoptotic role in human leukemic K562 cells exposed to ionizing radiation.

    abstract::Exposure of cells to ionizing radiation (IR) determines cellular lesions, such as DNA and membrane damage, which involve a coordinate network of signal transduction pathways responsible for resistance to or delay of apoptosis, depending on cell type and administered dose. Since, after IR exposure, the apoptotic profil...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cataldi A,Rapino M,Centurione L,Sabatini N,Grifone G,Garaci F,Rana R

    更新日期:2003-08-01 00:00:00

  • Prereplicative increase of nuclear matrix-bound DNA polymerase-alpha and primase activities in HeLa S3 cells following dilution of long-term cultures.

    abstract::We investigated the association of DNA polymerase and DNA primase activity with the nuclear matrix in HeLa S3 cells diluted with fresh medium after having been cultured without any medium change for 7 days. Flow cytometric analysis demonstrated that just before dilution about 85% of the cells were in the G1 phase of t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Martelli AM,Capitani S,Neri LM

    更新日期:1998-10-01 00:00:00

  • Identification of pathway-based recurrence-associated signatures in optimally debulked patients with serous ovarian cancer.

    abstract::Serous ovarian cancer (SOC) is the most common form of the histological subtype of epithelial ovarian cancer, with the worst clinical outcome. Despite improvements in surgery and chemotherapy, most patients with SOC experience recurrence within 12-18 months of first-line treatment. Current studies are unable to robust...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Deng K,Zhang F,Song W,Zhao W,Rong Z,Cai Y,Xu H,Lu M,Wang W,Li A,Hou Y,Li Z,Li K

    更新日期:2018-11-01 00:00:00

  • EGF Protects Cells Against Dox-Induced Growth Arrest Through Activating Cyclin D1 Expression.

    abstract::It has been reported that the antitumor drug doxorubicin (Dox) exerts its toxic effects via GATA-4 depletion and that over-expression of GATA-4 reverses Dox-induced toxicity and apoptosis; however, the precise mechanisms remain unclear. In this study, we observed, for the first time, that EGF protects cells against Do...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yao CX,Shi JC,Ma CX,Xiong CJ,Song YL,Zhang SF,Zhang SF,Zang MX,Xue LX

    更新日期:2015-08-01 00:00:00

  • Hormone-dependent processing of the avian progesterone receptor.

    abstract::Avian progesterone receptor exists as two forms, A and B, with molecular weights of 79,000 and 110,000 daltons, respectively. The origin and significance of these two forms is an area of active investigation and debate. Monoclonal antibodies produced against these two forms were used to examine receptor stability in c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sullivan WP,Smith DF,Beito TG,Krco CJ,Toft DO

    更新日期:1988-02-01 00:00:00

  • Metformin inhibits tumorigenesis in HBV-induced hepatocellular carcinoma by suppressing HULC overexpression caused by HBX.

    abstract::We aimed to understand whether metformin imposes the inhibitory effect on the HBV-associated tumorigenesis by regulating the HULC and its downstream signaling pathway. Luciferase assay, RT-PCR, and Western-blot, MTT and flow cytometry analysis were performed to understand and the mechanism, by which metformin enhance ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Jiang Z,Liu H

    更新日期:2018-06-01 00:00:00

  • Modulation of O-GlcNAc glycosylation during Xenopus oocyte maturation.

    abstract::O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a post-translational modification, which is believed antagonises phosphorylation. We have studied the O-GlcNAc level during Xenopus oocyte meiotic resumption, taking advantage of the high synchrony of this model which is dependent upon a burst of phosphorylation...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lefebvre T,Baert F,Bodart JF,Flament S,Michalski JC,Vilain JP

    更新日期:2004-11-15 00:00:00

  • Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV).

    abstract::Weightlessness induces bone loss in humans and animal models. We employed the NASA-approved Rotating Wall Vessel bioreactor (RWV) to develop osteoblast-like cell cultures under microgravity and evaluate osteoblast phenotype and cell function. Rat osteoblast-like cell line (ROS.SMER#14) was grown in the RWV at a calcul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Rucci N,Migliaccio S,Zani BM,Taranta A,Teti A

    更新日期:2002-01-01 00:00:00

  • Hemolymph-dependent and -independent responses in Drosophila immune tissue.

    abstract::Insects possess an antimicrobial defense response that is similar to the mammalian innate immune response. The innate immune system is designed to recognize conserved components of microorganisms called pathogen-associated molecular patterns (PAMPs). How host receptors detect PAMPs and transmit the signals to mount th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Bettencourt R,Asha H,Dearolf C,Ip YT

    更新日期:2004-07-01 00:00:00

  • Overlapped differentially expressed genes between acute lymphoblastic leukemia and chronic lymphocytic leukemia revealed potential key genes and pathways involved in leukemia.

    abstract::Common differentially expressed genes (DEGs) in acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL) might play critical roles in the pathogenesis and process of leukemia. We collected RNA sequencing (RNA-seq) data of human CLL, ALL samples, and normal peripheral blood CD19+ B cells as well as thy...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhang S,Zhang Q,Yin J,Wu X

    更新日期:2019-09-01 00:00:00

  • Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1).

    abstract::Pyrimidine-specific ribonucleases are a superfamily of structurally related enzymes with distinct catalytic and biological properties. We used a combination of enzymatic and non-enzymatic assays to investigate the release of such enzymes by isolated cells in serum-free and serum-containing media. We found that human e...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Landré JB,Hewett PW,Olivot JM,Friedl P,Ko Y,Sachinidis A,Moenner M

    更新日期:2002-01-01 00:00:00

  • Metabolic Oscillations in Co-Cultures of Hepatocytes and Mesenchymal Stem Cells: Effects of Seeding Arrangement and Culture Mixing.

    abstract::In vitro assembly of functional liver tissue is a prerequisite for the transplantation of tissue-engineered livers. There is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of implantable liver systems is currently limited by the difficulty of ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Alzebdeh DA,Matthew HW

    更新日期:2017-09-01 00:00:00

  • Energy metabolism characterization of a novel cancer stem cell-like line 3AB-OS.

    abstract::Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutic target, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells, exhibit a reduced mitochondrial acti...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Palorini R,Votta G,Balestrieri C,Monestiroli A,Olivieri S,Vento R,Chiaradonna F

    更新日期:2014-02-01 00:00:00