Abstract:
:The cadherin/catenin complex plays a key role in the initiation of cell-cell recognition, and adhesion, and the elaboration of structural and functional organization in multicellular tissues and organs. It is associated with tumor metastasis and also acts as an "invasion suppressor" of cancer cells. Nasopharyngeal carcinoma (NPC) is notorious for its highly metastatic nature. The expression of the E-cadherin/catenin complex is down-regulated in NPC tumor specimens. To obtain better insight into the intercellular adhesive property of NPC cells, we used immunofluorescence microscopy, immunoprecipitation, and immunoblot analysis to examine the expression of the classical cadherins and beta-catenin in a NPC cell line, TW-039. The results demonstrate a change in the distribution of E-cadherin from cytosolic flakes to cell-cell contacts with increasing time in culture. Between days 1 and 5 after plating, the detergent-insoluble fraction of E-cadherin increased from 20% to 37% of total E-cadherin, and that for P-cadherin increased from 33% to 40%. By contrast, the values for beta-catenin remained unchanged (26% and 25%). Both immunofluorescence and immunoblot studies suggested that P-cadherin may be involved in pioneer contact adhesion of TW-039 cells. Interestingly, E-, P-, and N-cadherin are co-expressed in this cell line. Immunoprecipitation studies also showed that other members of the cadherin family may be involved in the contact adhesion of TW-039 cells.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Lou PJ,Chen WP,Lin CT,DePhilip RM,Wu JCsubject
Has Abstractpub_date
1999-11-01 00:00:00pages
161-72issue
1eissn
0730-2312issn
1097-4644pii
10.1002/(SICI)1097-4644(20000101)76:1<161::AID-JCBjournal_volume
76pub_type
杂志文章abstract::Procyanidins have been associated with a reduced risk of cardiovascular diseases such as atherosclerosis. However, the molecular mechanisms underlying this benefit are not fully understood. Increased reactive oxygen species (ROS) production generated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is a ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24011
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abstract::The proteins of 14-3-3 family are substantially involved in the regulation of many biological processes including the apoptosis. We studied the changes in the expression of five 14-3-3 isoforms (beta, gamma, epsilon, tau, and zeta) during the apoptosis of JURL-MK1 and K562 cells. The expression level of all these prot...
journal_title:Journal of cellular biochemistry
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abstract::Enhanced phosphorylation of the ribosomal protein s6 kinase, p70(s6k), and the translational repressor, 4E-BP1, are associated with either insulin-induced or amino acid-induced protein synthesis. Hyperphosphorylation of p70(s6k) and 4E-BP1 in response to insulin or amino acids is mediated through the mammalian target ...
journal_title:Journal of cellular biochemistry
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abstract::Endogenous peptides play crucial roles in various biological processes. Their effects on the pathogenesis of human infantile hemangioma remains poorly understood. In this study, we construct a comparative peptidomic profiling between human infantile hemangioma tissue and matched normal skin using liquid chromatography...
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pub_type: 临床试验,杂志文章
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abstract::Amplification of MYCN is one of the most important prognostic markers for neuroblastoma and is correlated with rapid tumor progression and poor prognosis. MYCN belongs to the Myc/Max/Mad/Mnt network of proteins that regulate proliferation, apoptosis, and differentiation. It is well established that MYCN is downregulat...
journal_title:Journal of cellular biochemistry
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更新日期:2004-08-15 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1992-05-01 00:00:00
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journal_title:Journal of cellular biochemistry
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doi:10.1002/jcb.28444
更新日期:2019-02-14 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/(sici)1097-4644(20000201)76:2<341::aid-jcb
更新日期:1999-12-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most common type of cancer. MicroRNAs have been reported to participate in the progression of various cancers. In previous studies, miR-301a-3p expression was shown to be upregulated in CRC tissues. However, the underlying mechanism of miR-301a-3p in CRC has not yet been elucidated...
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pub_type: 杂志文章
doi:10.1002/jcb.27894
更新日期:2019-04-01 00:00:00
abstract::Cellular life-span of neonatal human brain microvascular endotheliocytes (HBME) was estimated by population doubling levels (PDLs) for serial subcultivations until spontaneous proliferation stoppage, and was 2.4-fold longer for continuous administration with the 6-O-phosphorylated derivative (TocP) of alpha-tocopherol...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2007-10-15 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::Serological proteins of neuroblastoma were profiled and analyzed by ProteinChip-SELDI-TOF MS technology with five types of protein chips. By comparing with normal control, a number of protein or polypeptide signals were found significantly and consistently different in their intensities (expression levels) in tumor se...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20417
更新日期:2005-05-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25422
更新日期:2016-06-01 00:00:00
abstract::We have previously shown that protein kinase Cε (PKCε) is involved in mitochondrial dysfunction in renal proximal tubular cells (RPTC). This study examined mitochondrial targets of active PKCε in RPTC injured by the model oxidant tert-butyl hydroperoxide (TBHP). TBHP exposure augmented the levels of phosphorylated (ac...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27256
更新日期:2018-11-01 00:00:00
abstract::The initiation of DNA replication in T lymphocytes appears to be regulated by two distinct activities: one associated with proliferation which mediates initiation, and another associated with quiescence which blocks initiation. Activated lymphocytes and proliferating lymphoid cell lines produce an activity, termed ADR...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240450107
更新日期:1991-01-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.10475
更新日期:2003-05-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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journal_title:Journal of cellular biochemistry
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更新日期:2004-03-01 00:00:00
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更新日期:2013-05-01 00:00:00
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journal_title:Journal of cellular biochemistry
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Journal of cellular biochemistry
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更新日期:2020-10-01 00:00:00
abstract::The activity of S6 kinases (S6K) is highly induced in cancer cells highlighting an essential role in carcinogenesis. The S6K family has two members: S6K1 and S6K2 which bear common as well as distinct features. In an attempt to identify S6K2 unique sequence features compared to S6K1, we applied extensive bioinformatic...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
abstract::Molecular mechanisms responsible for tumor resistance to apoptosis often involve the Fas/FasL pathway. While squamous cell carcinomas of the head and neck (SCCHN) express both Fas and FasL, their resistance to self-induced apoptosis or apoptosis mediated by Fas agonistic antibody (CH-11Ab) was independent of the level...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
abstract::Sea urchin CS histone variants are electrophoretically heterogeneous when analyzed in two dimensional polyacrylamide gels (2D-PAGE). Previous results suggested that this heterogeneity is due to the poly (ADP-ribosylation) of these proteins. Consequently, native CS histone variants were subjected to different treatment...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.12
更新日期:1996-04-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240520204
更新日期:1993-06-01 00:00:00
abstract::The sex steroid hormones, androgens and estrogens, via their respective nuclear receptors, regulate bone mineral density in humans and mice. Very little is known about the direct targets of the androgen and estrogen receptors in bone cells. First, models of hormone and receptor deficiency in mouse and human bone are d...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.22970
更新日期:2011-02-01 00:00:00