Abstract:
:The trout thyroid secretes L-thyroxine (T4) which undergoes enzymatic deiodination in liver and other tissues. Based on mammalian studies, T4 outer-ring deiodination (ORD) or T4 inner-ring deiodination (IRD) could generate respectively 3,5,3'-triiodo-L-thyronine (T3) or 3,3',5'-T3(rT3), while subsequent T3ORD or T3IRD could generate respectively 3,5-diiodo-L-thyronine (T2) or 3,3'-T2, and rT3ORD or rT3IRD could generate respectively 3,3'-T2 or 3',5'-T2. In practice, T4 in trout undergoes hepatic ORD to produce T3 but negligible IRD to produce rT3, and T3 in turn undergoes negligible ORD but modest IRD to produce 3,3'-T2. T4ORD, which is particularly important in converting T4 to the biologically more potent T3, also occurs in gill, muscle and kidney. At least two isozymes are involved: i) a high-affinity, propylthiouracil (PTU)-sensitive T4ORD which displays ping-pong kinetics, requires thiol as a cofactor, and is present in liver, gill and muscle, and ii) a low-affinity, PTU-insensitive T4ORD with sequential kinetics with a thiol cofactor, and is present in liver and kidney. Receptor-bound T3 is derived primarily from the plasma for kidney, mainly from intracellular sources for gill and about equally from both plasma and intracellular sources for liver. Thus, the high-affinity T4ORD may produce T3 for local intracellular use while the low-affinity 5'-monodeiodinase may produce T3 for systemic use. T4ORD activity responds to nutritional factors and the physiologic state of the fish. Furthermore, T3 administered orally for either 6 weeks or 24h reduces the functional level (Vmax) of hepatic T4ORD, and T3 added to isolated hepatocytes also reduces activity, indicating direct T3 autoregulation of T4ORD to maintain hepatocyte T3 homeostasis. However, T3 administration also induces T4IRD to produce biologically inactive rT3 and induces T3IRD to produce 3,3'-T2. Thus, the trout liver has several iodothyronine deiodinase systems which in a coordinated manner regulate tissue T3 homeostasis in the face of a T3 challenge. It does this by decreasing formation of T3 itself, by diverting T4 substrate to biologically inactive rT3 and by increasing the degradation of T3. These deiodinases differ in many respects from any mammalian counterparts.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Eales JG,Maclatchy DL,Sweeting RMdoi
10.1007/BF00004580subject
Has Abstractpub_date
1993-07-01 00:00:00pages
313-21issue
1-6eissn
0920-1742issn
1573-5168journal_volume
11pub_type
杂志文章abstract::The aims of the present study are to determine whether triiodothyronine (T3) and/or thyroxine (T4) in tilapia larvae is gifted through the mother, and to investigate the change profiles of thyrotropin (TSH), thyroid follicular cells and type I deiodinase (D1) gene expression following larval development. T3 and T4 con...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9950-7
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abstract::The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9431-6
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abstract::Cells from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce OPs varying from 300 to 500 mOsm kg(-1) and the direct effects of OP (salinity) on the fatty acid compositions of the main glyc...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00002456
更新日期:1995-04-01 00:00:00
abstract::Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...
journal_title:Fish physiology and biochemistry
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doi:10.1007/s10695-011-9548-2
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abstract::The sperm duct epithelium of brook trout (Salvelinus fontinalis), mountedin vitro in Ussing-style epithelial chambers actively absorbs Na(+) (measured as the short-circuit current, Isc) and secretes K(+) (measured using(86)Rb(+) as tracer). Dibutyryl-cyclic-adenosine monophosphate (db-cAMP) and 3-isobutyl-1-methylxant...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004725
更新日期:1989-06-01 00:00:00
abstract::The purpose of the current study was to examine seasonal changes in seawater tolerance and growth performance of anadromous Arctic charr (Salvelinus alpinus L.) held at the same temperature (8°C) during winter and summer. Charr (20-27 cm), previously reared in freshwater under natural photoperiod, were transferred eit...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004413
更新日期:1993-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0164-4
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00630-9
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abstract::A completely randomized experimental design carried out to investigate the effects of different levels of Pediococcus acidilactici (PA) including 0 (basal diet as a control diet), 1 × 106, 2 × 106, 4 × 106, and 8 × 106 colony-forming unit (CFU) per gram of the diet for 60 days on the mucosal immunity responses, growth...
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abstract::In order to specify the timing of some changes in ovarian steroid production during the transition from vitellogenesis to ovulation, plasma hormones levels andin vivo andin vitro responses of the ovary to salmon gonadotropin (s-GtH) or dibutyryl-cyclic adenosine mono-phosphate (db-cAMP) were recorded in relationship w...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02264076
更新日期:1986-10-01 00:00:00
abstract::The serum carotenoid level gradually increased during the spawning migration of chum salmon (Oncorhynchus keta). Zeaxanthin, a yellowish carotenoid, was detected in the serum of spent males, while astaxanthin was a main carotenoid in the serum of males and females at other developmental stages. A high bilirubin level ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875644
更新日期:1988-05-01 00:00:00
abstract::Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-009-9355-1
更新日期:2010-06-01 00:00:00
abstract::Mineral needs as affected by changes in dietary protein and oil sources were studied in rainbow trout. Duplicate groups (n = 30 fish per replicate) of rainbow trout (initial BW: 37 g) were fed either a fish meal/fish oil-based (M) or a complete plant ingredient (V)-based diet at four graded ration (R) levels [apparent...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-0020-y
更新日期:2015-02-01 00:00:00
abstract::Individual differences in behaviour and physiological responses to stress are associated with evolutionary adaptive variation and thus raw material for evolution. In farmed animals, the interest in consistent trait associations, i.e. coping styles, has increased dramatically over the last years. However, one of limita...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0231-5
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00818-4
更新日期:2020-10-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0074-5
更新日期:2015-10-01 00:00:00
abstract::An embryonic stem (ES)-like cell culture system RESC from a commercially important freshwater carp, Labeo rohita, was developed using blastula stage embryos. The cells were cultured in Leibovitz-15 (L-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basi...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9674-5
更新日期:2012-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02265140
更新日期:1991-06-01 00:00:00
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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更新日期:1986-09-01 00:00:00
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journal_title:Fish physiology and biochemistry
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更新日期:2009-08-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:Fish physiology and biochemistry
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更新日期:2016-04-01 00:00:00
abstract::Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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更新日期:1990-11-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9975-y
更新日期:2014-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004327
更新日期:1994-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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更新日期:2020-11-13 00:00:00
abstract::Vitellogenin (VTG) from spotted wolffish, Anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by MgCl₂/EDTA precipitation followed by a two-step chromatographic procedure. VTG had an apparent molecular mass of 470 kDa, as determined by gel filtration, and an amino acid composition sim...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
abstract::Using a tame animal, the impact of otter (Lutra lutra) disturbance on over-wintering carp (Cyprinus carpio) was monitored in two experiments, 133 and 140 days, respectively, over two consecutive winters (November-April). The level of stress in over-wintering carp exposed to various intensities of disturbance by otters...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-007-9180-3
更新日期:2008-09-01 00:00:00