Abstract：
:Malignant gliomas are the most common central nervous system tumors and the molecular mechanism driving their development and recurrence is still largely unknown, limiting the treatment of this disease. Here, we show that restoring the expression of miR-218, a microRNA commonly downregulated in glioma, dramatically reduces the migration, invasion, and proliferation of glioma cells. Quantitative reverse transcription PCR and Western blotting analysis revealed that expression of the stem cell-promoting oncogene Bmi1 was decreased after overexpression of miR-218 in glioma cells. Mechanistic investigations defined Bmi1 as a functional downstream target of miR-218 through which miR-218 ablated cell migration and proliferation. We documented that miR-218 also blocked the self-renewal of glioma stem-like cells, consistent with the suggested role of Bmi1 in stem cell growth. Finally, we showed that miR-218 regulated a broad range of genes involved in glioma cell development, including Wnt pathways that suppress glioma cell stem-like qualities. Taken together, our findings reveal miR-218 as a tumor suppressor that prevents migration, invasion, proliferation, and stem-like qualities in glioma cells.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Tu Y,Gao X,Li G,Fu H,Cui D,Liu H,Jin W,Zhang Ydoi
10.1158/0008-5472.CAN-13-0358subject
Has Abstractpub_date
2013-10-01 00:00:00pages
6046-55issue
19eissn
0008-5472issn
1538-7445pii
0008-5472.CAN-13-0358journal_volume
73pub_type
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