Abstract：
:We report our experience with a method to evaluate binding and uptake in cells of low density lipoprotein (LDL) from heterozygous patients with familial defective apolipoprotein B-100 (FDB-LDL) and LDL from normolipidemic subjects (nonFDB-LDL). The method is based on competition for binding/uptake in Epstein-Barr Virus (EBV)-transformed lymphocytes or COS cells overexpressing an LDL-receptor transgene between fluorescently labeled LDL and the unlabeled LDL of interest, and measurements are by flow cytometry. With EBV-lymphoblasts, the ability of FDB-LDL to displace fluorescent LDL ("Dil"-LDL) from cells at 4 degrees C (binding) was reduced to approximately 1/3 of normal. Displacement of "Dil"-LDL by FDB-LDL from cells at 20 degrees C (binding/uptake) was reduced to less than 1/2 of normal. Similar results were obtained with COS cells. Freezing of serum to -80 degrees C for 24 hours did not affect results, and we could discriminate between binding/uptake of FDB-LDL and nonFDB-LDL prepared from serum that had been stored at -80 degrees C for three months.
journal_name
Z Gastroenteroljournal_title
Zeitschrift fur Gastroenterologieauthors
Heath F,Jensen TG,Hansen PS,Jensen LG,Vissinger H,Moestrup SK,Pedersen S,Kølvraa S,Bolund L,Faergeman Osubject
Has Abstractpub_date
1996-06-01 00:00:00pages
21-4eissn
0044-2771issn
1439-7803journal_volume
34 Suppl 3pub_type
杂志文章abstract：:A 62-year-old lady presented with fever and abdominal pain. History revealed cholecystectomy two years ago due to cholecystolithiasis, complicated by perforation of the common bile duct, leading to hepaticojejunostomy, jejunocholedochostomy, endoscopic retrograde insertion of two plastic stents and percutaneous draina...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-2005-858973
更新日期：2006-04-01 00:00:00
abstract：:Probiotics are defined as living micro-organisms which, when administered in large amounts, confer a health benefit on the host. The use of probiotics in the therapy of infectious bowel diseases as well as maintaining remission of ulcerative colitis and in pouchitis is evidence-based. Also, in several studies proof co...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,meta分析
doi：10.1055/s-2004-813934
更新日期：2005-05-01 00:00:00
abstract：:Reference values for B-mode abdominal ultrasound are controversially discussed in the literature mainly due to the lack of data and non-standardized examination techniques. A cohort of 100 patients was prospectively examined. Patients with hepatobiliary diseases including gallstones, acute or chronic pancreatitis (n =...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0033-1335175
更新日期：2013-04-01 00:00:00
abstract：:The mechanism of the diarrheogenic effect of bile acids and fatty acids is incompletely understood. In order to study their effects on motility we tested sodium deoxycholate and sodium ricinoleate in their actions on the spontaneous mechanical and myoelectrical activity of the isolated circular muscle of the cat colon...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:Modern metabolic research in hepatology employs non-invasive techniques of the whole organism, and it includes studying the intact organ. Following recent decades of efforts in culturing isolated cells and studying their properties separately, it has become clear that the spatiotemporal organisation of different cell ...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：10.1055/s-0031-1273392
更新日期：2011-06-01 00:00:00
abstract：:A 29-year-old man presented with abdominal cramps and bloody diarrhoea. Blood tests revealed elevated C-reactive protein (21.3 mg/dL; normal range 0.01 - 0. 82 mg/dL) and white blood cells (28200/μL, normal range 4000 - 10000/μL). Stool tests were negative for enteropathogenic bacteria and Clostridium difficile toxins...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0031-1299289
更新日期：2012-02-01 00:00:00
abstract：:Anisakiasis or "herring worm disease" is one of the most important parasitic diseases of the gastrointestinal tract in Japan. In 1988 Lorenz and Warzok published 8 cases of intestinal anisakiasis in Eastern Germany. In 1988 Spehn et al. reported a case of gastric anisakiasis in an AIDS patient. Here, we describe a cas...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-2001-11150
更新日期：2001-02-01 00:00:00
abstract：:This report presents a female patient suffering from chronic diarrhea, who developed palpable purpura on the lower extremities 8 weeks after onset of the gastrointestinal symptoms. Biopsies obtained from the colon and skin showed leukocytoclastic vasculitis. Possible triggers or underlying diseases could not be found,...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0032-1325470
更新日期：2013-01-01 00:00:00
abstract：:The effects of surgical and chemical sympathectomy on various gastric functions was studied in rats. From the results it is concluded that the sympatho-adrenal system inhibits gastric acid secretion and mucosal blood flow. The regulation of pepsin secretion by the sympathetic nervous system appears controversial: unde...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:The beneficial effects of ursodeoxycholic acid (UDCA) in primary biliary cirrhosis are supposed to be mediated in part by immunological mechanisms. In this study, we have tested whether ursodeoxycholic acid also ameliorates intestinal inflammation induced by indomethacin in rats. Intestinal inflammation was induced in...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:We present the case of a primary malignant esophageal melanoma arising in a 75-year-old male, initially diagnosed as anaplastic squamous cell carcinoma. After resection of the tumor, histological work-up was indicative of a marked morphological heterogeneity, resembling a focally amelanotic primary malignant melanoma....
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-2005-858102
更新日期：2005-06-01 00:00:00
abstract：:The metabolism of ethanol to acetaldehyde in the liver proceeds via alcohol dehydrogenase (ADH) and the microsomal ethanol-oxidizing system (MOS), whereas catalase plays no significant role. ADH is an enzyme of the cytosol, requires NAD+ as cofactor and exhibits a pH optimum in the alkaline range. The Km of ADH is abo...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：
更新日期：1988-10-01 00:00:00
abstract：:Non-resectable cholangiocarcinoma and gallbladder carcinoma have a poor prognosis. In addition to the general aspects of "best supportive care", biliary drainage is an important part of the palliative treatment of patients with malignant biliary stenosis. Photodynamic therapy has led to an improved median survival in ...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：10.1055/s-2006-926869
更新日期：2006-07-01 00:00:00
abstract：:We have assessed the effect of omeprazole (30 mg daily) on gastric acid secretion as well as on basal hormone levels (fasting gastrin; TSH, T3, T4, TBG; insulin, glucagon, C-peptide; prolactin, testosterone, 17-beta-oestradiol, dihydroepiandrosterone, cortisol and PTH) in 8 healthy volunteers before and after a 28 day...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:The distribution of class I (A, B, C) and class II (DR antigens) histocompatibility antigens (HLA) was examined in 82 patients with hepatocellular carcinoma (HCC) and in 147 patients with chronic liver disease as controls. The diagnosis of HCC was confirmed by histological examination of liver tissue. HLA-B15 antigen ...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Liver fibrosis is the final common pathway in a variety of liver diseases. To model liver fibrosis in mice is important as mechanisms not only of fibrogenesis, but also of fibrolysis, need to be clearly defined. Also, small rodents present a possibility to test potential treatments in vivo. Today, there are several mo...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：10.1055/s-2006-927387
更新日期：2007-01-01 00:00:00
abstract：:Pancreatic cancer is one of the most lethal cancer diseases. For years, gemcitabine has been the standard of care and the only therapeutic option in patients with metastatic pancreatic cancer. Within the last years, new combination therapies have been established for first-line treatment, which significantly improve o...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/a-0581-5640
更新日期：2018-06-01 00:00:00
abstract：:Laboratory diagnostics efficiently applied is of decisive importance for a great deal of questions in spite of the technical and endoscopic methods which are available today in the field of examinations. By means of a screening programme consisting of ALAT, gamma-GT, ChE at a sensitivity of > 90% alterations in both t...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：
更新日期：1993-02-01 00:00:00
abstract：:1. In conscious rats provided with indwelling pancreatic and biliary catheters, the influence of anesthesia was investigated. 2. Ether was applied in concentrations of 4, 6 and 8 Vol % by an evaporator and Na-pentobarbital injected intravenously (30 mg/kg b. w.) 3. Flow rate, electrolytes (ion-selective electrodes and...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Cystic fibrosis is the most common lethal genetic disease in Caucasian population. End-stage lung disease is the most frequent cause of death, however since therapeutic options have improved the life expectancy of patients with cystic fibrosis, increased incidences of gastrointestinal tumors including pancreatic carci...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0033-1356225
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Achalasia in childhood is rare, also the etiology and the pathogenesis of the early onset ort he disease is practically unknown. Little is known about the neuropathological changes in structure of the esophageal wall in non-hereditary, sporadic achalasia in children and ist differentiation to that in adults....
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章,评审
doi：10.1055/s-2007-963649
更新日期：2007-12-01 00:00:00
abstract：:Gastric cancer and cancer of the oesophagogastric junction is diagnosed in the locally advanced or metastatic setting in 55 - 65 % of the cases and is generally treated with perioperative or palliative chemotherapy. For patients with metastatic disease, it is important to reliably determine the HER2 status for potenti...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0043-116493
更新日期：2017-09-01 00:00:00
abstract：:We studied the effect of two peptides, the intracellular Ca2(+)-mobilizing caerulein and the cAMP-mediated secretin, and also the phosphodiesterase inhibitor caffeine on pancreatic secretion and growth in newborn rats. To investigate the secretory response of these substances, and to construct dose-response curves 10-...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：BACKGROUND:Following the introduction of the MELD score, the survival rates have worsened after liver transplantation (LTX) in Germany. Existing organ shortages, shorter survival rates after LTX, and failures in the liver allocation process provide true challenges. Facilitated by a structured questionnaire, the appropr...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0034-1366290
更新日期：2014-08-01 00:00:00
abstract：:12% of the rectal biopsies (19% of the patients) showed epitheloid cell granulomas in clinically definite Crohn's disease. In colonic biopsies such granulomata were visible in 14% (22% of the patients). Granulomata were scarcely seen in normal or slightly inflamed mucosa. They were mostly found together with crypt abs...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:A 69-year-old man presented for endoscopic examination of the upper gastrointestinal tract because of dysphagia for solid food and unintended weight loss. Several months before, he had noticed brownish-gray skin lesions in the neck, in the thorax and in both axillae. A dermatological consultant expressed the suspicion...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0031-1299503
更新日期：2012-07-01 00:00:00
abstract：:Pneumatosis intestinalis (PI) is identified as the accumulation of gas within the wall of the small or large intestine. It was first described by Du Vernoi in 1783. The pathogenesis is supposed to be multifactorial. It is assumed that there are 3 paths of disease development: mechanical, bacterial and biochemical. Adu...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0031-1281680
更新日期：2013-11-01 00:00:00
abstract：:A 34-year-old Turkish woman presented with septic fever, sweats, arthralgia, and abdominal pain. Further examination revealed generalized lymphadenopathy, hepatosplenomegaly, pancytopenia, and multiple caseous granulomas. Microbiological diagnosis revealed Brucella melitensis type 3 as causative agent. This case repor...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Cytomegalovirus (CMV) plays an important role in non-immunocompetent patients due to its high seroprevalence and life-long persistence. However, cases of severe CMV infections are also described in the immunocompetent. Here in particular, the gastrointestinal involvement is of major importance. We describe the case of...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：10.1055/s-0031-1273487
更新日期：2013-11-01 00:00:00
abstract：:The amino acid consumption test (AACT) during exogenous stimulation with secretin and CCK was proposed as a sensitive and highly specific test for detection of exocrine pancreatic insufficiency. To further investigate the diagnostic value of this test we measured the AACT in comparison with the pancreolauryl serum tes...
journal_title：Zeitschrift fur Gastroenterologie
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
