Abstract:
:A sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the N-terminal propeptide of human procollagen type I (PINP) utilizing purified alpha 1-chain specific rabbit antibodies is described. The ELISA measured the content of the alpha 1-chain of PINP independent of the molecular form of the molecule. A parallelism was found between amniotic fluid (calibrator), normal and patient serum, and purified PINP (alpha 1), as well as the high and low molecular weight forms of PINP (alpha 1). The concentration of PINP in the calibrator (second trimester amniotic fluid) was determined to 25 micrograms/mL and the detection limit was 62 pg/mL measured in amniotic fluid, and 41 pg/mL measured in serum. The interassay coefficients of variation were 4.6% (low control) and 5.3% (high control), and the corresponding intraassay parameters were 2.9% and 4.9%. Recovery studies revealed an accuracy between 93% and 105%. The normal range (n = 57) for PINP was 56 ng/mL (median) the 10th and 90th centiles being 30 and 82 ng/mL, respectively. Patients with hyperparathyroidism due to hypovitaminosis D had median serum level of 168 ng/mL with a 10th centile of 44 ng/mL and a 90th centile of 450 ng/mL, these values being significantly different from the normal range (p < 0.001). The PINP-ELISA was superior to commercially available assays for PICP and osteocalcin in separation between healthy controls and patients with osteomalaci.
journal_name
Bonejournal_title
Boneauthors
Orum O,Hansen M,Jensen CH,Sørensen HA,Jensen LB,Hørslev-Petersen K,Teisner Bdoi
10.1016/8756-3282(96)00165-2subject
Has Abstractpub_date
1996-08-01 00:00:00pages
157-63issue
2eissn
8756-3282issn
1873-2763pii
8756328296001652journal_volume
19pub_type
杂志文章相关文献
BONE文献大全abstract::In 1994, the World Health Organization published diagnostic criteria for osteoporosis. Since then, many new technologies have been developed for the measurement of bone mineral at multiple skeletal sites. The information provided by each assessment will describe the clinical characteristics, fracture risk and epidemio...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2007.11.001
更新日期:2008-03-01 00:00:00
abstract::CCN2/connective tissue growth factor (CCN2/CTGF) is known to promote both the proliferation and differentiation of chondrocytes, which actions are mediated by ERK and p38 MAPK, respectively. In this study, we first re-evaluated the involvement of multiple MAPKs therein and found that JNK also mediated such CCN2 signal...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.11.016
更新日期:2006-06-01 00:00:00
abstract::Postmenopausal fractures are associated with low bone mass; however, the role of low peak bone mass in young adults in determining subsequent osteoporosis suggests that premenopausal fractures may also be relevant. We therefore sought to determine whether a self-reported previous history of premenopausal wrist and non...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00778-0
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:Studies in childhood suggest that both body composition and early postnatal growth are associated with bone mineral density (BMD). However, little is known of the relationships between longitudinal changes in fat (FM) and lean mass (LM) and bone development in pre-pubertal children. We therefore investigated...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.02.015
更新日期:2015-06-01 00:00:00
abstract::The deformation mechanism of bone at different hierarchical levels has been of wide interest. The important features of bone, its anisotropy and orientation dependent deformation are equally important, which have also gained a long run discussion. Most of the studies are concentrated on protein-rich collagen fibres an...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.01.394
更新日期:2009-06-01 00:00:00
abstract::On the basis of studies with bone-seeking isotopes, it is generally believed that the metabolic activity of osteoblasts of the trabecular bone is greater than that of the osteoblasts of the cortex. This implies that the oxidative activity, which provides the energy for biosyntheses, will also be greater in the former ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(89)90061-6
更新日期:1989-01-01 00:00:00
abstract::Pseudoachondroplasia (PSACH), a severe short-limbed dwarfing condition, is associated with life-long joint pain and early onset osteoarthritis. PSACH is caused by mutations in cartilage oligomeric matrix protein (COMP), a pentameric matricellular protein expressed primarily in cartilage and other musculoskeletal tissu...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2017.03.045
更新日期:2017-09-01 00:00:00
abstract::Insulin-dependent type 1 diabetes mellitus (IDDM) has been shown to alter the properties of bone and impair bone repair in both humans and animals. The objective of this study was the detailed histomorphometric evaluation of the influence of the diabetic metabolic state on bone formation and remodeling during bone def...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.011
更新日期:2004-07-01 00:00:00
abstract::Proteins of the extracellular matrix often have multiple functions to facilitate complex tasks ranging from signaling to structural support. Here we have focused on the function of one of the matrix proteins expressed in bones and teeth, the matrix adhesion protein ameloblastin (AMBN). Transgenic mice with 5-fold elev...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.01.041
更新日期:2013-05-01 00:00:00
abstract::Fracture risk and mechanical competence of bone are functions of bone mass and tissue quality, which in turn are dependent on the bone's mechanical environment. Male mice have a greater response to non-weight-bearing exercise than females, resulting in larger, stronger bones compared with control animals. The aim of t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.12.002
更新日期:2007-04-01 00:00:00
abstract::In 979 healthy women, aged 30-75 years, bone mass was measured by DXA in the lumbar spine and proximal femur, and by SXA in the distal forearm. Bone turnover was assessed by urinary CrossLaps (CrossLaps ELISA), a new assay which measures type I collagen degradation products in urine and by osteocalcin (two-site N-Mid ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(96)00201-3
更新日期:1996-09-01 00:00:00
abstract:PURPOSE:To evaluate the effects of different definitions of glucocorticoid (GC) exposure on the magnitude and pattern of fracture risk using the same dataset. METHODS:Data from patients with rheumatoid arthritis (RA) were extracted from the Clinical Practice Research Datalink, a primary care database with electronic h...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.09.004
更新日期:2018-12-01 00:00:00
abstract:INTRODUCTION:Because osteoporotic patients are prone to fractures, it must be considered whether or not patients undergoing drug therapies should discontinue treatment after sustaining a non-vertebral fracture. This study has tested the effect of novel active vitamin D3 analog, 1alpha,25-dihydroxy-2beta(3-hydroxypropox...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.07.023
更新日期:2007-01-01 00:00:00
abstract::Endochondral ossification in the growth plate of long bones involves cartilage mineralization, bone formation and the budding vasculature. Many of these processes take place in a complex and dynamic zone, the provisional ossification zone, of the growth plate. Here we investigate aspects of mineralization in 2D and 3D...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115086
更新日期:2020-01-01 00:00:00
abstract::Androgen receptor (AR) is expressed throughout the osteoblast lineage. Two different AR transgenic families (AR2.3-tg and AR3.6-tg mice) demonstrating overlapping and distinct expression profiles were employed to assess the effects of enhanced androgen sensitivity to ameliorate hypogonadal loss. Two different paradigm...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.08.111
更新日期:2012-11-01 00:00:00
abstract::Diabetes increases the risk of fracture, impairs fracture healing and causes rapid loss of the fracture callus cartilage, which was linked to increased FOXO1 expression in chondrocytes. We recently demonstrated that deletion of FOXO1 in chondrocytes blocked the premature removal of cartilage associated with endochondr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.026
更新日期:2019-06-01 00:00:00
abstract::Bisphosphonates are a unique class of drugs. As a family, they are characterized pharmacologically by their ability to inhibit bone resorption, whereas, pharmacokinetically, they are classified by their similarity in absorption, distribution, and elimination. Although all bisphosphonates have similar physicochemical p...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/8756-3282(95)00445-9
更新日期:1996-02-01 00:00:00
abstract::Selective sodium-dependent glucose co-transporter 2 inhibitors (SGLT2Is) are oral hypoglycemic medications utilized increasingly in the medical management of hyperglycemia among persons with type 2 diabetes (T2D). Despite favorable effects on cardiovascular events, specific SGLT2Is have been associated with an increas...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115254
更新日期:2020-04-01 00:00:00
abstract::The use of combined antiretroviral agents during pregnancy is important to prevent mother-to-child transmission of human immunodeficiency virus (HIV). Antiretroviral treatment (ARV) is associated with reduced bone mass and altered bone metabolism in HIV-infected patients. There are no data regarding the effect of ARV ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.10.030
更新日期:2012-01-01 00:00:00
abstract::ED-71, a novel analog of 1alpha,25-(OH)2 D3, increases bone mass to a greater extent than alfacalcidol, an 1alpha,25-(OH)2 D3 prodrug. In this study, we used a murine bone marrow ablation model to compare the effect of ED-71 on bone formation and resorption in vivo with that of 1alpha,25-(OH)2 D3. We discovered that b...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.08.017
更新日期:2007-02-01 00:00:00
abstract::The lung interfaces with atmospheric oxygen via a large surface area and is perfused by the entire venous return bearing waste products collected from the whole body. It is logical that the lung is endowed with generous anti-oxidative capacity derived both locally and from the circulation. The single-pass pleiotropic ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2017.03.047
更新日期:2017-07-01 00:00:00
abstract::It is well established that short-term clearance of an intravenous calcium load in vivo reflects bone uptake. Using results from isotope-dilution experiments with 42Ca, a 3-h test has been developed to measure a quantity, gamma, related to bone accretion. This test is proposed as a useful, clinically applicable measur...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(94)00004-2
更新日期:1995-01-01 00:00:00
abstract::Profound bone loss at weight bearing sites is a primary effect of long-duration spaceflight. Moreover, a significant increase in estimated fracture risk remains even 1 year after returning to Earth; hence, it is important to define how quickly bone integrity can recover following prolonged disuse. This study character...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.09.021
更新日期:2013-01-01 00:00:00
abstract::There are increasing evidences suggesting bone marrow adiposity tissue (MAT) plays a critical role in affecting both bone quantity and quality. However, very limited studies that have investigated the association between the composition of MAT and bone mineral density (BMD). The goal of this study was to quantify MAT ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.08.014
更新日期:2017-12-01 00:00:00
abstract::Recent studies have indicated a critical role for vascular endothelial growth factor (VEGF) during the process of endochondral ossification, in particular in coupling cartilage resorption with bone formation. Therefore, we studied the chemoattractive and proliferative properties of human VEGF-A on primary human osteob...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00690-1
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Good bone and joint health is essential for the physical tasks of daily living and poorer indicators of physical capability in older adults have been associated with increased mortality rates. Genetic variants of indicators of bone and joint health may be associated with measures of physical capability. MET...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.10.004
更新日期:2013-01-01 00:00:00
abstract::Increased levels of reactive oxygen species (ROS) are a crucial pathogenic factor of osteoporosis. Gastrodin, isolated from the traditional Chinese herbal agent Gastrodia elata, is a potent antioxidant. We hypothesized that gastrodin demonstrates protective effects against osteoporosis by partially reducing reactive o...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.12.059
更新日期:2015-04-01 00:00:00
abstract::Among vertebrates, urodele amphibians (e.g., axolotls) have the unique ability to perfectly regenerate complex body parts after amputation. The limb has been the most widely studied due to the presence of three defined axes and its ease of manipulation. Hence, the limb has been chosen as a model to study the process o...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.07.005
更新日期:2007-01-01 00:00:00
abstract::Adiponectin regulates various metabolic processes including glucose flux, lipid breakdown and insulin response. We recently reported that adiponectin receptor1 (adipoR1) activation by a small molecule reverses osteopenia in leptin receptor deficient db/db (diabetic) mice. However, the role of adiponectin in bone metab...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.08.005
更新日期:2017-12-01 00:00:00
abstract::This study describes the early interface reaction of cancellous bone to a nanocrystalline hydroxyapatite cement containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD). Cylindrical implants of HA/Coll and it...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.11.019
更新日期:2007-04-01 00:00:00