Abstract:
:The CYP11B2 gene encodes aldosterone synthase, a cytochrome P450 (P450aldo) expressed in high levels in the adrenal zona glomerulosa. While the primary physiologic regulators of aldosterone production are circulating angiotensin II (Ang II) and potassium (K+) the action of these agents on CYP11B2 gene transcription have not been examined. Because these factors increase intracellular calcium we have hypothesized that calcium signaling pathways are one mechanism controlling CYP11B2 transcription. Previously we demonstrated that increases in intracellular calcium increase P450aldo mRNA. Herein, we analyzed the role of calcium in the expression of the human CYP11B2 gene using transient transfection of a luciferase reporter construct containing 2017 bp of human CYP11B2 5'flanking DNA in mouse Y-1 and human H295R adrenocortical cell lines. When transfected into Y-1 cells, reporter gene expression was increased following treatment with ACTH or forskolin, but not with Ang II, the L-type calcium channel agonist BAYK8644, or ionomycin. In H295R cells, however, reporter gene expression was increased following treatment with Ang II, K+, BAYK8644 ionomycin or dibutyryl cAMP (Bu2cAMP). Activation of protein kinase C with TPA did not alter reporter gene expression in either cell line. These data demonstrate that both calcium and cAMP signaling pathways regulate human CYP11B2 gene expression. In addition, the H295R adrenal cell line appears to be an appropriate model to study regulation of CYP11B2 by calcium.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Clyne CD,White PC,Rainey WEdoi
10.1080/07435809609043735subject
Has Abstractpub_date
1996-11-01 00:00:00pages
485-92issue
4eissn
0743-5800issn
1532-4206journal_volume
22pub_type
杂志文章abstract::Islets derived from patients with persistent hyperinsulinemic hypoglycemia of infancy, PHHI, show a significant capacity to proliferate in vitro without the addition of growth factors. However, as with other differentiated cells, PHHI-derived islet cells show a loss of differentiated function with repeated subculture....
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229235
更新日期:2005-01-01 00:00:00
abstract::The stimulation of the zona glomerulosa phenotype by a low sodium diet is characterised by increased expression of aldosterone synthase accompanied by increases in (pro)renin, bFGF, c-fos and c-jun gene transcription. In contrast ACTH diminishes the specific glomerulosa phenotype. The EGF related transmembrane protein...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032720
更新日期:1998-08-01 00:00:00
abstract::Purpose: Limited and contradictory data on the circulating levels of glucagon-like peptide (GLP-1) and resistin in hepatitis C virus genotype-4 (HCV-4) cirrhotic patients are present. Thus, this study aimed to evaluate their concentrations and to investigate the association between total GLP-1, resistin, and insulin r...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2019.1627551
更新日期:2020-02-01 00:00:00
abstract::The action of peptide hormones from the anterior pituitary regulates transcription of a large number of genes located in most, if not all, tissues. This action is mediated through regulation of steroid hormone production in the steroidogenic factories (adrenals, gonads). These steroid hormones are transported through ...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435809609043755
更新日期:1996-11-01 00:00:00
abstract::The rat adrenal cortex possesses at least two mechanisms for uptake of extracellular lipoprotein cholesterol; one is receptor mediated endocytosis and the second is a non-endocytotic pathway. The latter was revealed by the ability of lipoproteins such as HDL which do not possess apo B or apo E to enhance steroid hormo...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435808409036509
更新日期:1984-01-01 00:00:00
abstract::Steroid 21-hydroxylase deficiency is the most frequent cause of congenital adrenal hyperplasia, an inherited inability to synthesize cortisol. Mutations causing this disorder have been characterized by hybridization analysis of patient DNA samples using cDNA and oligonucleotide probes, and by cloning and sequencing of...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808909039099
更新日期:1989-01-01 00:00:00
abstract::The liver is known to be sensitive to dietary challenge and to reduction in its functional mass. To investigate the influence of the vitamin D endocrine system on the hepatic growth response, liver DNA synthesis (S phase of cell cycle) was primed by dietary manipulation (high carbohydrate/protein-free diet x 3 d follo...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809109106818
更新日期:1991-01-01 00:00:00
abstract::The adrenal cortex is a dynamic organ in which the cells of the outer cortex continually divide. It is well known that this cellular proliferation is dependent on constant stimulation from peptides derived from the ACTH precursor proopiomelanocortin (POMC) since disruption of pituitary corticotroph function results in...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016801
更新日期:2002-11-01 00:00:00
abstract:AIM:To determine whether hyperuricemia is associated with the early phase and late phase of insulin release in apparently healthy non-obese subjects with normal glucose tolerance. MATERIALS AND METHODS:A population-based cross-sectional study was performed. Apparently healthy individuals aged 20 to 65 years were eligi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1155597
更新日期:2017-02-01 00:00:00
abstract::Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120018678
更新日期:2003-02-01 00:00:00
abstract:PURPOSE:To determine whether daily oral micronized progesterone affects bone turnover, as estimated by serum and urine biochemical markers, in postmenopausal women on long-term estrogen replacement therapy (ERT). METHODS:We recruited 14 women aged 65 or older to participate in a 9-week trial with micronized progestero...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-120022294
更新日期:2003-05-01 00:00:00
abstract::Purpose: Transthyretin (TTR) is a protein with a growing number of biological functions in addition to its well-established binding and circulatory transport of thyroxine, and indirect retinoid transport through interaction with retinol-binding protein. Misfolded and aggregated wild-type and mutant TTRs are involved i...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2019.1694538
更新日期:2020-01-01 00:00:00
abstract:INTRODUCTION:Impairments in neuroendocrine regulation of food intake and postprandial satiety are leading causes to obesity. Ghrelin peptide is a GI hormone known to increase food intake partly through induction of growth hormone. The regulation of ghrelin production is still unknown. OBJECTIVE:The aim of the current ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.799484
更新日期:2014-01-01 00:00:00
abstract::A method is described for the preparation of high yields of viable, dissociated cells from porcine theca interna and corpus luteum and from human and bovine endometrium. The tissues were dissociated by incubation at 37 degrees C in a mixture of 0.5% collagenase, 0.1% hyaluronidase and 0.1% pronase in balanced salt sol...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808409035415
更新日期:1984-01-01 00:00:00
abstract::Tumor-promoting phorbol esters are believed to affect cell functions by activating a Ca+2-and lipid-dependent protein kinase (protein kinase C). 12-0-Tetradecanoyl phorbol-13-acetate (TPA) inhibits LH stimulation of progesterone (P) accumulation in rat granulosa cells. To determine the mechanisms by which TPA inhibits...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035438
更新日期:1986-01-01 00:00:00
abstract::Adrenal computed tomography with determination of Hounsfield units has proved to be sensitive and specific in the differential diagnosis of benign vs. malign adrenal lesions. On the other hand, computed tomography may fail in patients with small adrenal masses of less than 1.0 cm. However, especially in patients with ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120017075
更新日期:2002-11-01 00:00:00
abstract::We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107168
更新日期:2001-02-01 00:00:00
abstract::Type 1 angiotensin II (AII) receptors (AT1 receptors), besides stimulation of aldosterone secretion, seem to transduce the growth factor-like activity of AII on glomerulosa cells. Although a local renin-angiotensin system and AII synthesis have been found in human adrenals and aldosteronomas, it is unclear whether ald...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030434
更新日期:1995-02-01 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) levels were found to be associated with obesity, indicating that adipocytes might influence PAI-1 plasma levels. In addition, the 4G/5G promoter polymorphism of the PAI-1 gene possibly modulates PAI-1 gene transcription and, as a consequence, PAI-1 plasma levels. Metabolic par...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200035728
更新日期:2004-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glucose tolerance progressively declines with age, and there is a high prevalence of type 2 diabetes in the elderly people. Previous studies have reported the sex differences in risk for type 2 diabetes, especially in the elderly people, whereas reasons for these sex differences remain poorly understood...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1124437
更新日期:2016-08-01 00:00:00
abstract::The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. O...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030443
更新日期:1995-02-01 00:00:00
abstract:PURPOSE:The aim of the present study was to investigate the association between plasma homocysteine (Hcy) levels and carotid, cardiac, and renal end-organ damage in newly diagnosed type 2 diabetes mellitus (T2DM) patients. METHODS:Newly diagnosed normotensive T2DM patients (n = 390) were enrolled in this study. The pa...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1171235
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1141936
更新日期:2016-11-01 00:00:00
abstract::The enzyme, 3 beta-hydroxysteroid dehydrogenase/delta 5-4 isomerase (3 beta-HSD) is an essential element in the biosynthetic pathway for potent adrenal steroid hormones that appear to regulate maturation of many tissues in utero and are critical for homeostasis after birth. The results of prior studies are suggestive ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030422
更新日期:1995-02-01 00:00:00
abstract::Y1 adrenocortical tumor cells possess amplified and overexpressed c-Ki-ras proto-oncogene, displaying chronic high levels of the c-Ki-Ras-GTP protein. Despite this oncogenic lesion, we previously reported that Y1 cells retain tight regulatory mechanisms of cell cycle control typified by the mitogenic response triggere...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043600
更新日期:2004-11-01 00:00:00
abstract::The recent cloning of a family of melanocortin receptors (MC-R) has identified five distinct G protein- and adenylate cyclase-coupled receptors. The MC2-receptor (MC2-R) preferentially binds ACTH. It is expressed in the adrenal cortex and is hence considered to be the ACTH receptor. The MC5-receptor (MC5-R) binds ACTH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032628
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND:Small dense low-density lipoprotein (sd-LDL) has been demonstrated to be associated with cardiovascular diseases (CVD). The proposed atherogenic properties of hypoadiponectinemia might be mediated through increased sd-LDL. In this study, the associations of sd-LDL with cardiovascular and other complications ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2014.913614
更新日期:2015-01-01 00:00:00
abstract::A study was conducted to provide information on the characteristics of skeletal muscle glucocorticoid receptors and to determine the response of serum cortisol and thyroxine secretion to colostrum feeding in the neonatal calf. Twenty-four calves (12 males and 12 females) were obtained immediately postpartum and random...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808809036341
更新日期:1988-01-01 00:00:00
abstract::Using Western blot analysis we found transcriptional co-repressor Sin3A to be expressed at a higher level in male mouse kidney than in females. HDAC1 (histone deacetylase 1) protein, another co-repressor forming complexes with Sin3A, was not higher in males. No sex differences in Sin3A expression were found after gona...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229243
更新日期:2005-01-01 00:00:00
abstract::Basal serum concentrations of prolactin (PRL) and the elevation of serum PRL concentrations induced by the serotonergic agents, quipazine and 5-hydroxytryptophan (5-HTP) were suppressed in rats treated acutely with dexamethasone. Acute dexamethasone treatment did not alter the stimulation of PRL release produced by th...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808709023671
更新日期:1987-01-01 00:00:00